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"ABSTRACTPenelitian ini dilakukan untuk menganalisis pembentukan DNA Adduct 8-OHdG akibat kerusakan oksidatif DNA yang disebabkan oleh paparan formaldehida dan logam Cu (II). Studi in vivo dilakukan dengan menggunakan kelompok tikus putih (Rattus norvegicus) yang diberi paparan formaldehida (82 mg/kg BB) dan Cu (II) (10 mg/kg BB) selama 28 hari. Sampel urin diambil setiap minggunya. Studi in vitro dilakukan dengan mereaksikan 2-deoksiguanosin dengan formaldehida, logam Cu (II), dan H2O2 melalui reaksi Fenton-like. Reaksi dilakukan pada suhu 37°C dengan variasi pH (7,4 dan pH 8,4) serta waktu inkubasi (7 dan 12 jam). Analisis pembentukan 8-OHdG secara in vivo dan in vitro dilakukan menggunakan instrumen LC-MS/MS dengan kromatogafi fasa terbalik. Fasa gerak yang digunakan adalah campuran amonium asetat 20 mM pH 4 dan asetonitril dengan gadien elusi. Hasil dari penelitian menunjukkan bahwa paparan formaldehida dan logam Cu (II) dapat menyebabkan terbentuknya DNA Adduct 8-OHdG. Pada studi in vivo, ditemukan kadar 8-OHdG tertinggi pada kelompok paparan formaldehida dengan Cu (II). Pada studi in vitro, terbentuk 8-OHdG dengan konsentrasi paling tinggi pada kelompok variasi formaldehida, Cu (II) dan H2O2.

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ABSTRACTThis research was conducted to analyze the formation of DNA Adduct 8-OHdG due to oxidative DNA damage caused by exposure formaldehyde and Cu (II). In vivo studies were conducted using a group of rat (Rattus norvegicus) which were exposed to formaldehyde (82 mg/kg BW) and Cu (II) (10 mg/kg BW) for 28 days. Urin samples were taken every week. In vitro studies were carried out by reacting 2-deoxyguanosine with formaldehyde, Cu (II) and H2O2 through a Fenton-like reaction. The reaction was carried out at 37°C with variation in pH (7,4 and 8,4) and incubation time (7 and 12 hours). Analysis of the formation DNA Adduct 8-OHdG with in vivo and in vitro studies using LC-MS/MS with reverse phase chromatogaphy. The mobile phase used was a mixture of 20 mM ammonium acetate pH 4 and acetonitrile with elution gadient. The results of the study show that exposure of formaldehyde and Cu (II) can cause the formation of a DNA Adduct 8-OHdG. In vivo study showed that the highest levels of 8-OHdG were found in the group that exposed to formaldehyde with Cu (II). In vitro study showed that 8-OHdG was formed with the highest concentration in the formaldehyde, Cu (II) and H2O2 variation groups."

"Senyawa 3-monokloropropan-1,2-diol (3-MCPD) merupakan senyawa yang ternyata dapat ditemukan dalam produk minyak sawit. Senyawa xenobiotik ini berbahaya karena akumulasinya dalam tubuh dapat menyebabkan masalah kesehatan yang tidak ringan. Jika dibiarkan menumpuk dalam tubuh, 3-MCPD dapat merusak struktur DNA menyebabkan mutasi sel yang berujung pada kanker. Penelitian ini bertujuan untuk menganalisis terbentuknya DNA Adduct 8-hidroksi-2′-deoksiguanosin (8-OhdG) dan malondialdehida (MDA) akibat paparan senyawa 3-MCPD pada tikus Rattus novergicus. Paparan dilakukan selama 28 hari dengan medium minyak sawit RPMO fraksi olein yang diproses dengan metode enzimatis. Sampel yang akan digunakan berupa serum darah untuk keperluan analisis menggunakan ELISA dan colorimetric assay kit dan sampel dari organ hati, ginjal, usus, dan lambung untuk keperluan analisis histopatologi. Hasil analisis menujukan kadar 8-OHdG yang paling tinggi dan kerusakan organ ginjal yang sedang pada serum darah dari paparan 3-MCPD dosis tinggi.

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3-Monochloropropan-1,2-diol (3-MCPD) is a compound that has been identified in palm oil products. This xenobiotic substance poses significant health risks due to its potential to accumulate in the human body, which can lead to various adverse health effects. If this substance accumulates in the body, 3-MCPD can damage DNA structure, potentially leading to cell mutations that may result in cancer. The objective of this study is to analyze the formation of DNA adducts 8-hydroxy-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) as a result of exposure to the 3-MCPD compound in Rattus norvegicus rats. The exposure will be conducted over a period of 28 days using an RPMO palm oil olein fraction as a medium, processed via an enzymatic method. The samples will consist of blood serum for analysis using ELISA and colorimetric assay kits, as well as tissue samples from the liver, kidneys, intestines, and stomach for histopathological analysis. The results of the analysis showed that the highest levels of 8-OHdG and moderate kidney damage were obtained in blood serum from high-dose 3-MCPD exposure."