Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
Latar belakang: Propolis memiliki efek antifungal. Universitas Indonesia sedang mengembangkan permen mengandung propolis yang diduga dapat menghambat pertumbuhan C.albicans. Tujuan: Penelitian ini bertujuan untuk mengetahui efek ekstrak propolis dan permen propolis terhadap pertumbuhan C.albicans. Metode: Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimum (KBM) ekstrak propolis diuji menggunakan metode spektrofotometri serta jumlah koloni C.albicans pasca pemaparan ekstrak dan permen propolis dihitung secara langsung. Hasil: Nilai KHM dicapai pada konsentrasi 10%, nilai KBM dicapai pada konsentrasi 15%; serta terdapat penurunan jumlah koloni jamur pasca pemaparan ekstrak dan permen propolis. Kesimpulan: Ekstrak dan permen propolis terbukti efektif menghambat pertumbuhan C.albicans. ......Background: Propolis has antifungal effect. Universitas Indonesia has been developing propolis candy which can inhibit C.albicans growth. Objective: This research was aimed to analyze effect of propolis extract and candies to C.albicans growth. Methods: After C.albicans were exposed to propolis extract and candies, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined by spectrophotometry and post-exposure colonies of C.albicans were counted. Result: MIC of propolis extract against C.albicans were determined at 10% and MBC at 15%, also the amount of C.albicans colonies were decreased after propolis extract and candies exposure. Conclusion : Propolis extract and candies were effective to inhibit C.albicans growth.

Abstrak :
Salah satu faktor virulensi Candida albicans adalah kemampuannya dalam membentuk biofilm sehingga meningkatkan resistensi terhadap agen antifungal. Fase awal merupakan prasyarat terbentuknya biofilm serta ditandai dengan adhesi dan proliferasi sel C. albicans. Temulawak merupakan tanaman khas Indonesia dan dilaporkan memiliki efek antifungal karena mengandung zat aktif yaitu xanthorrhizol. Penelitian ini dilakukan dengan MTT assay untuk mengukur viabilitas C. albicans pada biofilm setelah pemaparan ekstrak etanol temulawak secara in vitro. Hasil penelitian menunjukkan ekstrak dengan konsentrasi 35% dapat menurunkan viabilitas C. albicans setara dengan nystatin. Ekstrak etanol temulawak terbukti memiliki efek antifungal terhadap C. albicans pada biofilm fase adhesi dan proliferasi. ...... Candida albicans has the ability to form biofilm that increase resistance to antifungal agents. Early phase is a prerequisite, characterized by adhesion and proliferation. Java turmeric is an Indonesian medicinal plants and reported to have antifungal effect due to its active component, xanthorrhizol. This study was conducted using MTT assay to measure viability of C. albicans in biofilm after exposure to Java Turmeric ethanol extract. The result showed extract in 35% concentration can reduce the viability of C. albicans equal to nystatin’s capability. Java Turmeric ethanol extract has antifungal effect against C. albicans in adhesion and proliferation phase of biofilm.

Abstrak :
Latar belakang: Temulawak yang mengandung xanthorrhizol diketahui memiliki efek antijamur. Xanthorrhizol dilaporkan mampu mengeradikasi biofilm Candida albicans. Tujuan: Menganalisis korelasi antara efek hambat ekstrak etanol temulawak EET dengan perkembangan biofilm C. albicans isolat klinis pada berbagai fase, serta mengamati gambaran mikroskopis biofilm C. albicans. Metode: Uji MTT digunakan untuk menguji viabilitas C. albicans pada biofilm dan dikonversikan persen hambat ekstrak etanol temulawak KHBM50 . Efek EET terhadap gambaran mikroskopis setiap fase perkembangan biofilm C. albicans diamati dengan Scanning Electron Microscopy. Hasil: Nilai Konsentrasi Hambat Biofilm Minimal KHBM50 EET terhadap biofilm C. albicans isolat klinis pada fase awal, menengah, dan maturasi secara berturut-turut adalah 20 , 30 , dan 35 . Gambaran mikroskopis pada setiap fase perkembangan biofilm C. albicans terlihat penurunan jumlah sel dan densitas C. albicans, serta terhambatnya pembentukan filamen dibandingkan dengan kelompok tanpa perlakuan. Kesimpulan: EET mampu menghambat perkembangan fase awal, menengah, dan maturasi biofilm C. albicans isolat klinis. Semakin matur fase perkembangan biofilm, C. albicans akan semakin resisten terhadap ekstrak temulawak. Paparan ekstrak temulawak memengaruhi kemampuan C. albicans isolat klinis dalam membentuk filamen serta menurunkan jumlah sel dan densitas biofilm. ...... Background: Javanese turmeric which contains xanthorrhizol is known to have antifungal effect. Xanthorrhizol is reported to be able to eradicate Candida albicans' biofilm formation. Objective: Analyze the correlation between inhibition concentration of Javanese turmeric ethanol extract JTEE and each development phase of C. albicans' biofilm, and observing microscopic appearance of each phase of C. albicans biofilm. Method: MTT assay was used to test the viability of C. albicans towards biofilm and converted to Minimum Biofilm Inhibitory Concentration MBIC50 . JTEE' s effect on each phase of microscopic appearance of C. albicans' biofilm is observed by Scanning Electron Microscopy. Result: MBIC50 of JTEE towards development of clinical isolate of C. albicans' biofilm in the early adhesion and proliferation , intermediate, and maturation phase as follows 20, 30, and 35 respectively. The microscopic appearance on each phase of C. albicans' biofilm development shows decrease in cell number and density, as well as inhibiton of filament formation compared with control group. Conclusion: JTEE can inhibit the development phases of C. albicans' biofilm. The potency of JTEE to inhibit development of C. albicans' biofilm was decreased along with the maturation of biofilm. The JTEE' s exposure leads to changes of microscopic appearance of C. albicans' biofilm development.

Abstrak :
Salah satu peran protein saliva adalah sebagai sistem pertahanan rongga mulut. Tujuan: Menganalisis efek protein saliva spesifik dan non-spesifik C. albicans dari kelompok usia anak, dewasa, lansia dalam pembentukan biofilm E. faecalis in vitro. Metode: Uji biofilm dengan crystal violet. Hasil: Pembentukan biofilm E. faecalis pada protein spesifik C. albicans dari kelompok dewasa dan lansia mengalami penurunan saat inkubasi 18 jam (p≤0.05). Pembentukan biofilm E. faecalis pada protein non-spesifik C. albicans dari ketiga kelompok usia mengalami peningkatkan saat inkubasi 6 jam dan 18 jam (p≤0.05). Kesimpulan: Protein spesifik C. albicans pada kelompok dewasa dan lansia menurunkan pertumbuhan bakteri E. faecalis. Protein non-spesifik C. albicans meningkatkan adhesi dan pertumbuhan bakteri E. faecalis.

---

One of the function of salivary proteins, it works as the first line of defense in the oral cavity. Objectives: to analyse the effect of specific and non specific salivary protein to C. albicans from children, adults and elderly on E. faecalis biofilm formation in vitro. Methods: Crystal violet assay. Results: Biofilm formation of E. faecalis on specific salivary protein to C. albicans, from adults and elderly, decreases when incubated for 18 hours (p≤0.05). Biofilm formation of E. faecalis on non-spesific salivary protein to C. albicans, from children, adults and elderly, increases when incubated for 6 hours and 18 hours (p≤0.05). Conclusion: Spesific salivary protein to C. albicans, from adults and elderly, decreases the growth of E. faecalis. Non-spesific salivary protein to C. albicans increases the adhesion and growth of E. faecalis.

Abstrak :
ECC terjadi akibat pembentukan biofilm Mutans streptococci dan Candida albicans yang sangat dipengaruhi oleh kualitas saliva. Objektif: Menganalisis pengaruh saliva autolog stimulated yang diisolasi dari ECC terhadap pembentukan biofilm Mutans streptococci dan Candida albicans secara in vitro. Metode: Uji biofilm Mutans streptococci dan Candida albicans ATCC 10231 dengan atau tanpa saliva autolog stimulated dilakukan dengan crystal violet binding assay. Hasil: Terdapat perbedaan antara pembentukan biofilm Mutans streptococci, Candida albicans dan multi-species dengan atau tanpa saliva, namun dengan uji t-test tidak berbeda bermakna (p>0,05). Kesimpulan: Saliva autolog stimulated mempengaruhi pembentukan biofilm Mutans streptococci dan Candida albicans serta ada kecendrungan pembentukan biofilm lebih tinggi pada multi-species. ......ECC occurs as a result of Mutans streptococci and Candida albicans which is influenced by the quality of saliva. Objective: Analyze the effects of stimulated autolog saliva that isolated from ECC to the biofilm formation Mutans streptococci and Candida albicans in vitro. Methods: Mutans streptococci and Candida albicans ATCC 10231 biofilm with the application of stimulated autolog saliva was tested using crystal violet binding assay. Result: There were differences of Mutans streptococci, Candida albicans and multi-species biofilm mass formation with or without saliva, but with t-test not significantly different (p>0,05). Conclusion: Autolog saliva influences Mutans streptococci and Candida albicans biofilm formation and there is a tendency of higher multi-species biofilm formation.