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"ABSTRAKTelah dilakukan penelitian untuk mengetahui pengaruh pemberian fortifikanNaFeEDTA dalam tepung tempe dan susu kedelai terhadap kadar natrium plasmadarah tikus (Rattus norvegicus L.) jantan galur Sprague Dawley. Sebanyak 25ekor tikus dibagi ke dalam 5 kelompok: kelompok kontrol 1 (KK1) yang diberiCMC 0,5%, kelompok kontrol 2 (KK2) yang diberi tepung tempe atau susukedelai, dan tiga kelompok perlakuan (KP1, KP2, KP3) yang diberi tepung tempeatau susu kedelai dengan fortifikan NaFeEDTA dosis 1,35 mg Fe/ kgBB; 2,7 mgFe/ kgBB; 5,4 mg Fe/ kgBB selama 21 hari berturut-turut. Penentuan kadarnatrium plasma dengan alat AES (Atomic Emission Spectroscopy). Hasil ujiAnava satu arah (P > 0,05) menunjukkan tidak ada pengaruh nyata pemberianfortifikan NaFeEDTA dalam tepung tempe dan susu kedelai terhadap kadarnatrium antar kelompok perlakuan. Kadar natrium plasma pada T21 dengan bahanuji tepung tempe dan susu kedelai tetap berada pada rentang normal antara 0,456mg/ml -- 0,586 mg/ml.

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ABSTRACTThe effect of NaFeEDTA fortificant inserted in tempeh flour and soy milk intakeon plasma sodium concentration in male Sprague-Dawley rats (Rattus norvegicusL.) had been studied. Twenty five rats were divided into five groups: controlgroup 1 (KK1) was administered with CMC 0.5%, control group 2 (KK2) wasadministered with tempeh flour or soy milk; three treatment groups (KP1, KP2,KP3) were administered with tempeh flour or soy milk added with fortificantNaFeEDTA 1.35 mg Fe/kgBw; 2.7 mg Fe/kgBw; 5.4 mg Fe/kgBw consecutivefor 21 days. Plasma sodium concentration was measured by AES (AtomicEmission Spectroscopy). One way Anova test (P > 0.05) showed there is nosignificant effect of fortificant NaFeEDTA inserted in tempeh flour and soy milkintake on plasma sodium concentration in all treatment groups. Plasma sodiumconcentration on T21 which was administered with tempeh flour and soy milkremains in normal range between 0.456 mg/ml ? 0.586 mg/ml."

"ABSTRAKTelah dilakukan penelitian yang bertujuan untuk mengetahui pengaruh pemberianfortifikan Fe Fumarat dalam tepung tempe terhadap kadar zat besi plasma darahtikus (Rattus norvegicus L.) jantan galur Sprague-Dawley. Metode penelitianmenggunakan Rancangan Acak Lengkap (RAL), terdiri atas 25 ekor tikus putihjantan yang dibagi ke dalam 5 kelompok perlakuan, yaitu KK 1 yang diberilarutan CMC 0,5%; KK 2 yang diberi CMC 0,5% dan suspensi tepung tempetanpa fortifikan; dan KP 1, 2, dan 3 yang diberi CMC 0,5% dan tepung tempedengan fortifikan Fe Fumarat dosis 1,35 mg Fe/ kgBB, 2,7 mg Fe/ kg BB, dan 5,4mg Fe/ kgBB selama 21 hari berturut-turut. Pengambilan darah dilakukan padahari ke-0 dan setelah pencekokan hari ke-21. Darah ditentukan kadar zat besinyadengan AAS (Atomic Absorption Spectrophotometer). Hasil uji Anava satu arah(P < 0,05) menunjukkan perbedaan nyata pemberian fortifikan Fe Fumarat dalamtepung tempe terhadap kadar zat besi antar kelompok perlakuan. Peningkatankadar zat besi tertinggi terjadi pada KP 1 yaitu sebesar 13,21% terhadap KK 1 dan11,48% terhadap KK 2.

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ABSTRACTThe effect of Fe fumarate fortificant addition in tempeh flour on plasma ironconcentration of male Sprague-Dawley rats (Rattus norvegicus L.) had beenstudied. By using Complete Random Design (CRD), twenty five rats were dividedinto five groups, consist of normal control group (KK 1) which was administeredwith CMC 0.5%, treatment control group (KK 2) which was administered withCMC 0.5% and tempeh flour non fortificant, and three treatment groups whichwere administered with tempeh flour added with Fe Fumarate fortificant 1.35 mgFe/KgBW (KP 1); 2.7 mg Fe/KgBW (KP 2); and 5.4 mg Fe/KgBW (KP 3). All ofthe five groups were treated within 21 consecutive days. The plasma ironconcentration was measured by Atomic Absorption Spectrophotometer (AAS).One way ANOVA test ( P < 0.05) showed significant effect of Fe Fumaratefortificant addition in tempeh flour intake on plasma iron concentration in alltreatment groups. The highest increase of plasma iron concentration was detectedon KP 1 which is 13.21% to KK 1 and 11.48% to KK 2."