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Abstrak :
Kambing Saanen merupakan salah satu kambing perah penghasil susu yang baik. Guna meningkatkan efisiensi reproduksi kambing Saanen, dikembangkan teknologi inseminasi buatan (IB). Salah satu faktor pendukung keberhasilan IB yaitu tersedianya semen beku sesuai kriteria. Tujuan dari penelitian yaitu mengetahui pengencer semen yang optimal untuk semen beku kambing Saanen dengan membandingkan pengencer semen berbasis lesitin (Andromed®) dan liposom (Optixcell®) dengan penambahan maltosa 0,4%. Metode koleksi semen dilakukan dengan menggunakan vagina buatan. Semen kambing Saanen kemudian diperiksa secara makroskopis dan mikroskopis. Teknik penyimpanan semen beku dengan teknik kriopreservasi pada suhu -196ºC selama 10 menit. Evaluasi kualitas semen meliputi motilitas, viabilitas, dan membran plasma utuh (MPU). Hasil yang diperoleh diuji secara statistik dengan uji ANAVA satu faktor kemudian uji Tukey. Hasil penelitian menunjukkan tidak terdapat perbedaan nyata (P>0,05) pada nilai rata-rata persentase motilitas perlakuan Andromed® (50,83±3,76); Andromed® + Maltosa 0,4% (50,83±3,76); Optixcell® (50,83±3,76); Optixcell® + Maltosa 0,4% (48,33±6,06), nilai rata-rata persentase viabilitas Andromed® (54,67±3,50); Andromed® + Maltosa 0,4% (54,50±2,51); Optixcell® (56,50±3,45); Optixcell® + Maltosa 0,4%(52,83±5,78), dan nilai rata-rata persentase MPU spermatozoa Andromed® (56,00±3,80); Andromed® + Maltosa 0,4% (56,50±5,47); Optixcell® (53,83±5,31); Optixcell® + Maltosa 0,4% (53,33±6,06). Hasil penelitian menyimpulkan bahwa pengencer semen liposom menghasilkan kualitas yang sama baik dengan pengencer semen berbasis lesitin dan penambahan maltosa 0,4% pada pengencer semen berbasis liposom dan lesitin tidak berpengaruh terhadap kualitas semen beku kambing Saanen yang dihasilkan ......The Saanen goat is a notable domestic goat that performs nicely in dairy production, such as milk. Efforts to improve the reproduction efficiency of Saanen goats, include the development of artificial insemination (IB) technology. A significant factor that effect AI is the availability of frozen semen according to satisfactory criteria. This research aims to determine the optimal semen extender for Saanen goat frozen semen by comparing lecithin‒based extender (Andromed) and liposomes (Optixcell) when added 0.4% maltose. Semen collection utilises artificial vagina, then is examined macroscopically and microscopically. The semen storage technique used in this study was the cryopreservation technique at -196 for 10 minutes. Criteria of semen quality includes motility, viability, and intact plasma membrane. The result obtained were statistically tested with the ANAVA one way factor test then Tukey test. The result showed that there was no significant difference (P>0.05) in the average value of the motility percentage of treatments Andromed (50.83±3.76); Andromed + Maltosa 0.4% (50.83±3.76); Optixcell (50.83±3.76); Optixcell + Maltosa 0.4% (48.33±6.06), viability Andromed (54.67±3.50); Andromed + Maltosa 0.4% (54.50±2.51); Optixcell (56.50±3.45); Optixcell + Maltosa 0.4% (52.83±5.78), and intact plasma membrane Andromed® (56.00±3.80); Andromed + Maltosa 0.4% (56.50±5.47); Optixcell (53.83±5.31); Optixcell + Maltosa 0.4% (53.33±6.06). The results of the study concluded that the liposomes extender produced the same quality as the lecithin-based extender and the addition of 0.4% maltose in liposomes and lecithin‒based extender had no effect on the quality of the frozen semen Saanen goat.

Abstrak :
Kambing saanen merupakan jenis kambing penghasil susu yang potensial dikembangkan di Indonesia. Keberhasilan inseminasi buatan (IB) dengan semen beku sangat dipengaruhi oleh kualitas semen beku. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan maltosa pada tiga konsentrasi berbeda (0,2%, 0,4%, dan 0,6%) ke dalam pengencer semen berbasis lesitin (Andromed®) terhadap kualitas spermatozoa kambing saanen selama kriopreservasi. Metode penampungan semen menggunakan vagina buatan, semen kambing saanen diperiksa secara makroskopis dan mikroskopis. Teknik penyimpanan semen beku dengan teknik kriopreservasi pada suhu -196ºC selama 10 menit. Evaluasi kualitas semen meliputi viabilitas dan membran plasma utuh. Hasil yang diperoleh diuji secara statistik dengan uji ANAVA satu faktor. Hasil penelitian menunjukkan tidak terdapat perbedaan nyata (P>0,05) pada nilai rata-rata persentase viabilitas dan membran plasma utuh pascathawing. Nilai rata-rata persentase viabilitas pascathawing pada perlakuan Andromed® + Maltosa 0% (54,67±3,50%); Andromed® + Maltosa 0,2% (53,57±3,88%); Andromed® + Maltosa 0,4% (54,50±2,51%); Andromed® + Maltosa 0,6% (51,67±3,39%). Nilai rata-rata persentase membran plasma utuh pascathawing pada perlakuan Andromed® + Maltosa 0% (56,00±3,80%); Andromed® + Maltosa 0,2% (55,50±4,23%); Andromed® + Maltosa 0,4% (56,50±5,47%); Andromed® + Maltosa 0,6% (54,83±3,35%). Hasil Penelitian menyimpulkan bahwa penambahan maltosa dalam pengencer semen berbasis lesitin (Andromed®) tidak memiliki pengaruh dalam mempertahankan kualitas spermatozoa kambing saanen selama kriopreservasi. ......Saanen goat is a type of goat that produces milk that potential to be developed in Indonesia. The success of artificial insemination (IB) with frozen semen is strongly influenced by the quality of frozen semen. This study aims to determine the effect of adding maltose at three different concentrations (0.2%, 0.4%, and 0.6%) to lecithin-based cement diluent (Andromed®) on the quality of saanen goat spermatozoa during cryopreservation. The semen collection method used an artificial vagina, and the semen of the saanen goat was examined macroscopically and microscopically. The technique for frozen semen storage was cryopreservation at -196ºC for 10 minutes. Evaluation of semen quality included viability and intact plasma membrane. The results obtained were tested statistically by one factor ANOVA test. The results showed that there was no significant difference (P> 0.05) in the mean value of the percentage of viability and intact plasma membrane post-thawing. The mean value of the percentage of post-thaw viability in the Andromed® + Maltose 0% (54.67 ± 3.50%); Andromed® + Maltose 0.2% (53.57 ± 3.88%); Andromed® + Maltose 0.4% (54.50 ± 2.51%); Andromed® + Maltose 0.6% (51.67 ± 3.39%). The mean value of post-thawing intact plasma membrane percentage in Andromed® + Maltose 0% (56.00 ± 3.80%); Andromed® + Maltose 0.2% (55.50 ± 4.23%); Andromed® + Maltose 0.4% (56.50 ± 5.47%); Andromed® + Maltose 0.6% (54.83 ± 3.35%). The results of the study conclude that the addition of maltose in lecithin-based cement diluent (Andromed®) did not affect in maintaining the spermatozoa quality of the saanen goat during cryopreservation.