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"Latar Belakang: Penentuan jenis kelamin penting untuk identifikasi forensik. Salah satu metodenya berdasarkan ukuran gigi. Tujuan: Mengetahui perbedaan ukuran gigi laki-laki dan perempuan serta menentukan nilai referensi gigi molar satu rahang atas untuk penentuan jenis kelamin. Metode: 30 gigi molar satu rahang atas laki-laki dan 30 perempuan diukur lebar mesiodistal dan bukolingual dengan kaliper digital. Hasil: Perbedaan signifikan (p<0,05) ukuran gigi molar satu rahang atas laki-laki dan perempuan. Nilai referensi ukuran bukolingual 11.34 mm (kanan), 11.22 mm (kiri); ukuran mesiodistal 10.61 mm (kanan) 10.51 mm (kiri). Kesimpulan: Ukuran mahkota gigi molar satu rahang atas dapat digunakan untuk penentuan jenis kelamin. ......Background: Sex determination is an important aspect in the human identification. One of the methods is using tooth dimensions. Objective: To obtain the differences of male and female tooth size using maxillary first molar crown dimensions and to determine reference point for sex determination. Methods: 30 males and 30 females, on maxillary first molar study cast. Mesiodistal and buccolingual width were measured using digital calipers. Results: The differences between males and females in all dimensions measured were statistically significant (p<0,05). The reference point for buccolingual width was 11.34 mm (right), 11.22 mm (left); for mesiodistal width was 10.61 mm (right) and 10.51 mm (left). Conclusion: Maxillary first molar crown dimension may be used as an aid in sex determination."