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Domba merupakan salah satu ternak unggulan di Kab. Purwakarta, Jawa Barat,....

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The previous studies had shown that Indonesian thin-tailed (ITT) sheep are more resistant to infection with fasciola gigantica than the Merino sheep....

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Telah dilakukan penelitian untuk mengetahui pengaruh sari buah jeruk siam Citrus nobilis Lour. dalam pengencer tris kuning telur TKT terhadap kualitas spermatozoa domba garut Ovis aries L. 24 jam pascakriopreservasi. Semen ditampung dari lima pejantan domba garut satu kali dalam satu minggu menggunakan vagina buatan. Segera setelah dievaluasi, semen diencerkan dengan pengencer tris kuning telur yang mengandung sari buah jeruk 0 KK, 5 KP 1, 10 KP 2, 15 KP 3, dan 20 KP 4. Semen dikemas dalam straw 0,25 ml dengan konsentrasi akhir 50x106 spermatozoa/ml. Semen diekuilibrasi pada suhu 5oC selama dua jam kemudian dibekukan dan disimpan dalam tabung N2 cair. Semen dievaluasi terhadap parameter motilitas, viabilitas, integritas membran, integritas akrosom, dan abnormalitas. Hasil uji ANAVA satu arah menunjukkan terdapat perbedaan nyata antar kelompok perlakuan terhadap persentase motilitas, viabilitas, dan integritas membran p < 0,05. Hasil uji lanjut Duncan menunjukkan bahwa KP 2 memiliki perbedaan nyata terhadap semua kelompok perlakuan. Berdasarkan hasil tersebut, penambahan sari buah jeruk pada konsentrasi 10 KP 2 merupakan konsentrasi terbaik yang mampu memperkecil penurunan kualitas spermatozoa domba garut pascakriopreservasi berdasarkan persentase motilitas 58,68 0,68, viabilitas 59,73 6,47, dan integritas membran 54,87 5,58. ...... The research aimed to find out the effect of siam orange juice in extender on garut ram spermatozoa quality 24 hours postcryopreseration. Semen was collected from five rams once a week using artificial vagina. Semen sample was diluted in tris egg yolk based extender containing 0 KK, 5 KP 1, 10 KP 2, 15 KP 3 and 20 KP 4 siam orange juice. Semen was packed in 0.25 ml straw with a final concentration of 50x106 spermatozoa ml. Sperm was equilibrated at 5 C for two hours then frozen and stored in a liquid nitrogen N2 tube. Sperm parameters including motility, viability, membrane integrity, acrosome integrity, and abnormality were assessed. One way ANAVA test results showed significant differences between treatment groups on the percentage of motility, viability, and membrane integrity.

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Telah dilakukan penelitian tentang penambahan antioksidan glutathione ke dalam medium pembekuan lambat terhadap kualitas oosit domba garut (Ovis aries) pascakriopreservasi. Tujuan dari penelitian ini adalah untuk mengevaluasi pengaruh penambahan antioksidan glutathione dalam media pembekuan lambat dengan konsentrasi 0,5 mM; 1 mM; 1,5 mM melawan kualitas oosit domba garut. Penelitian dilakukan di Lab. Reproduksi, Pemuliaan dan Kultur Sel Hewan LIPI, Cibinong. Kriopreservasi oosit dari 136 oosit dilakukan menggunakan krioprotektan 10% etilen glikol dan sukrosa 0,1 M. Evaluasi oosit dilakukan setelah 7 hari penyimpanan dalam nitrogen cair (-196°C), meliputi: morfologi oosit dan viabilitas oosit menggunakan pewarna Hoechst dan propidium pewarna iodida. Berdasarkan hasil penelitian didapatkan persentase oosit normal normal pascakriopreservasi yaitu 0 mM (68,40%), 0,5 mM (66,98%), 1 mM (73,05%), dan 1,5 mM (80,58%). Persentase oosit yang layak pasca-kriopreservasi adalah 0 mM (68,40%), 0,5 mM (69,76%), 1 mM (75,55%), dan 1,5 mM (83,08%). Berdasarkan uji statistik ANOVA, didapatkan hasil bahwa tidak ada perbedaan yang signifikan antar kelompok perlakuan (P > 0,05), namun grafik persentase menunjukkan pola yang cenderung meningkat dengan penambahan konsentrasi antioksidan glutathione. Kesimpulan penelitian berdasarkan hasil yang diperoleh yaitu penambahan antioksidan glutathione dalam media pembekuan lambat tidak berpengaruh terhadap kualitas oosit domba garut pasca-kriopreservasi.
Research has been carried out on the addition of glutathione as an antioxidant in slow freezing medium on the quality of post-cryopreserved arrowroot sheep (Ovis aries) oocytes. The aim of this study was to evaluate the effect of adding the antioxidant glutathione in slow freezing medium with a concentration of 0.5 mM; 1 mM; 1.5 mM against arrowroot sheep oocyte quality. The research was conducted in the Lab. Animal Cell Reproduction, Breeding and Culture LIPI, Cibinong. Oocyte cryopreservation of 136 oocytes was performed using cryoprotectant 10% ethylene glycol and 0.1 M sucrose. Evaluation of oocytes was carried out after 7 days of storage in liquid nitrogen (-196°C), including: oocyte morphology and oocyte viability using Hoechst stain and propidium iodide dye. Based on the results, the percentage of post-cryopreserved normal oocytes was 0 mM (68.40%), 0.5 mM (66.98%), 1 mM (73.05%), and 1.5 mM (80.58%). . The percentage of viable post-cryopreservation oocytes were 0 mM (68.40%), 0.5 mM (69.76%), 1 mM (75.55%), and 1.5 mM (83.08%). Based on the ANOVA statistical test, it was found that there was no significant difference between the treatment groups (P > 0.05), but the percentage graph shows a pattern that tends to increased with the addition of the antioxidant glutathione concentration. The conclusion of the study based on the results obtained was that the addition of the antioxidant glutathione in the slow freezing medium had no effect on the post-cryopreservation quality of arrowroot sheep oocytes.

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Penelitian ini menganalisis pengaruh pemberian sukrosa pada visualisasi inti oosit domba garut (Ovis aries) pascamaturasi dan pascakriopreservasi. Koleksi ovarium domba garut dilakukan di rumah potong hewan Sentul, Jawa Barat. Slicing ovarium dilakukan untuk dikoleksi oositnya. Grading dilakukan pada oosit yang terkoleksi. Oosit dengan gradeA dan B dimaturasi menggunakan media maturasi TCM-199 dan diinkubasi selama minimal 24 jam di dalam inkubator. Total jumlah oosit yang telah mencapai metafase II (M II) setelah maturasi dibagi menjadi dua untuk dilanjutkan ke tahap kriopreservasi dalam waktu minimal satu minggu dan untuk diberi perlakuan inkubasi pada media sukrosa dengan konsentrasi 0%, 1%, 3%, dan 5% dalam waktu ±10 menit persetiap perlakuan. Setelah diberi perlakuan, oosit diamati pembengkakan pada bagian intinya dengan menggunakan mikroskop fluoresens. Hasil uji statistik Kruskal-wallis menunjukkan (P ≤ 0,05). Konsentrasi sukrosa 3% menjadi konsentrasi yang optimum untuk menginduksi pembengkakan inti oosit domba garut pascamaturasi dan pascakriopreservasi. ......This study analyzed the effect of sucrose addition on sheep oocyte (Ovis aries) for visualization of oocyte nucleus after maturation and post-cryopreservation. Sheep ovaries was collected from rumah potong hewan Sentul, Jawa Barat. Ovaries were sliced and oocyte was collected from ovaries. The collected oocytes were grading. Grade A and B oocytes were matured using maturation media TCM-199 and incubated for at least 24 hours in the incubator. The total number of oocytes that have reached metaphase II (M II) after maturation is divided into two for development to the cryopreservation stage in at least one week and to be given an incubation treatment on sucrose media with concentrations of 0%, 1%, 3%, and 5% in time ± 10 minutes per each treatment. After being treated, the oocyte was observed for swelling at its core using a fluorescence microscope. Kruskal-wallis test showed that (P ≤ 0,05). The concentration of sucrose 3% becomes the optimal concentration to induce swelling of the oocyte core of garut sheep after maturation and post-cryopreservation.

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Kuntjoro A, Sutarno, Astirin OP. 2009. Body weight and statistic vital of Texel sheep in Wonosobo District by giving the ramie hay as an additional woof. Nusantara Bioscience 1: 23-30. This research is aimed to observe the body weight and statistic vital measurement of 50 Texel sheep. Sheep are classified into five treatments of giving woof P0 (giving tree greenish woof without concentrate), P1 (giving greenish woof and concentrate without adding the ramie hay/0%) concentrate), P2 (giving greenish woof and concentrate by adding 10%) ramie hay), P3 (giving greenish woof and concentrate by adding 20%) ramie hay), P4 (giving greenish woof and concentrate by adding 30%) ramie hay), every treatment was repeated 10 times. The result shows that even it can?t yet replace the concentrate function, but adding ramie hay as much as 10%), 20%) and 30%) on sheep woof can increase the body weight?s growth respectively 186.67 g/day, 153.34 g/day dan 103.34 g/day. The addition of ramie hay 10%), 20%) and 30%) can increase the addition of statistic vital?s measurement on breast of sheep livestock 1.20 cm); 0.95 cm) and 0.90 cm); the addition of statistic vital measurement on the body length of sheep livestock 0.05 cm); 1.00 cm) and 0.75 cm) and also the addition of breast width is 1.50 cm); 0.15 cm) and 0.3 cm). Meanwhile the addition of ramie hay on livestock woof can only increase the addition of statistic vital mesurement on breast at giving 30%) as big as 0.15 cm). It is needed to know further on giving ramie hay by concentration comparasion of hay of different leaf and stem.

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Goats and sheep are valuable as they are a source of meat, milk, fleece, and other products. These livestock are also important both for agriculture and biomedical research. However, the efficient, sustainable, and profitable production of these small ruminants faces major obstacles. Hence, this review analyzes these major challenges specifically, their negative impacts on the industry, and suggests some science-based solutions to overcome them. Those challenged areas are education and training, research, translational research/biotechnology, goat and sheep health, and maintenance of an economically sustainable agribusiness. The suggested solutions include the effective teaching of goat and sheep science to the next generation and public empowerment, support for innovative and translational research, disease prevention and treatment, support for technology transfer, and development of sound agribusiness practices. This review is helpful particularly for scientists, students, and the goat and sheep producers. In general, these information on the current state of goat and sheep agriculture will also help the public to better understand and appreciate the challenges met and opportunities provided in small ruminant production enterprises.

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Penelitian telah dilakukan pada oosit domba garut dengan menambahkan α-tokoferol ke dalam media vitrifikasi oosit dengan tujuan mengevaluasi penambahan α-tokoferol di dalam media vitrifikasi terhadap kualitas oosit domba garut. Oosit dikumpulkan & dimatangkan in vitro selama 24 jam pada suhu 38,5 ° C dan 5% CO2 kemudian di vitrifikasi menggunakan 15% EG, 15% DMSO dan sukrosa 0,5 M dan dibagi menjadi 4 kelompok: kelompok tanpa penambahan α- tokoferol (KK); dan tiga kelompok dengan penambahan berbagai konsentrasi α-tokoferol: 100 μM (KP1); 150 μM (KP2) dan 200 μM (KP3). Oosit disimpan dalam LN2 (-196 ° C) selama 7 hari dan dicairkan kembali setelahnya untuk mengevaluasi mereka menggunakan fluoresensi mikroskop dengan pewarna Hoechst & PI. Oosit normal jika mereka bulat, memiliki zona pelusida utuh, sitoplasma homogen; dan layak jika mereka memiliki warna biru pendaran. Evaluasi morfologi menunjukkan bahwa 69,40% (KK); 70.28% (KP1); 76,19% (KP2); dan 74,72% (KP3) dari total oosit vitrifikasi normal. Viabilitasnya evaluasi menunjukkan bahwa 75,52% (KK); 79,44% (KP1); 80,75% (KP2); 83,61% (KP3) dari total oosit vitrifikasi dapat hidup. Hasil statistik menunjukkan bahwa penambahan α- tokoferol dalam media vitrifikasi tidak memberikan perbedaan yang signifikan kelompok perlakuan (P> 0,05). Berdasarkan persentase, oosit normal tertinggi adalah diperoleh di KP2 dan oosit tertinggi di KP3. Hasil ini menunjukkan bahwa penambahan α-tokoferol dalam media vitrifikasi tidak berpengaruh terhadap kualitas oosit domba garut (P> 0,05). ...... Research has been carried out on arrowroot oocytes by adding α-tocopherol to the oocyte vitrification media with the aim of increasing α-tocopherol funds in vitrification media on the quality of arrowroot sheep oocytes. Oocytes were collected & matured in vitro for 24 hours at 38.5 ° C and 5% CO2 then vitrified using 15% EG, 15% DMSO and 0.5 M sucrose and divided into 4 groups: groups without α-tocopherol changes (KK); and three groups contributing various α-tocopherol concentrations: 100 μM (KP1); 150 μM (KP2) and 200 μM (KP3). Oocytes are stored in LN2 (-196° C) for 7 days and thawed afterwards to return they used a fluorescence microscope with Hoechst & PI coloring. Oocytes are normal if they are round, have intact zona pellucida, homogeneous cytoplasm; and feasible if they have blue luminescence. Morphological evaluation showed that 69.40% (KK); 70.28% (KP1); 76.19% (KP2); and 74.72% (KP3) of total normal vitrified oocytes. Viability Assessment shows 75.52% (KK); 79.44% (KP1); 80.75% (KP2); 83.61% (KP3) of total vitrified oocytes can live. Statistical results showed that administration of α-tocopherol in vitrified media did not provide a significant difference in the treatment group (P> 0.05). Based on the percentage, the highest normal oocyte is obtained at KP2 and the highest oocyte is at KP3. These results indicate that opposing α-tocopherol in vitrified media has no effect on the quality of arrowroot sheep oocytes (P> 0.05).

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Penelitian mengenai potensi antioksidan alfa-tokoferol terhadap tingkat maturasi dan kualitas oosit domba garut (Ovis aries) pascakriopreservasi menggunakan metode slow freezing telah dilakukan. Sebanyak 138 oosit yang memiliki kualitas A dan B dimaturasi dalam medium TCM-199 dengan penambahan antioksidan alfa-tokoferol pada konsentrasi 0 mM, 100 mM, 150 mM, dan 200 mM. Oosit hasil maturasi in vitro tersebut, kemudian dikriopreservasi serta diamati tingkat maturasi dan kualitasnya. Pengamatan tingkat maturasi dilihat berdasarkan pembentukan badan polar I. Pengamatan kualitas oosit meliputi kondisi morfologi dan viabilitas menggunakan pewarna Hoechst dan propidium iodide. Hasil penelitian menunjukkan bahwa tidak ada perbedaan signifikan antarkonsentrasi secara statistik (P > 0,05), namun terdapat kecenderungan konsentrasi 150 mM memiliki persentase tertinggi terhadap tingkat maturasi oosit in vitro (88,57%); viabilitas (82,86%); dan kondisi morfologi (82,86%). Dengan demikian, 150 mM alfa-tokoferol merupakan konsentrasi terbaik yang mampu mempertahankan stabilitas oosit pascakriopreservasi.

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The study of the potential alpha-tocopherol antioxidants towards garut sheep (Ovis aries) oocyte maturation and quality post-cryopreservation using slow freezing method has been conducted. A total of 138 oocytes which have A and B qualities, were maturating in TCM-199 medium with the addition of alpha-tocopherol antioxidants at concentrations of 0 mM, 100 mM, 150 mM, and 200 mM. Oocytes from in vitro maturation are then cryopreserved and the maturity and quality was observed. Observation of oocyte maturation was seen based on the formation of the polar body I. Observation of oocytes quality includes morphological condition and viability by Hoechst and propidium iodide dyes. This experiment showed that there was no statistically siginificant difference between concentrations (P > 0.05), but there is a tendency of 150 mM have the highest percentage of oocyte in vitro maturation (88.57%); viability (82.86%); and morphological condition (82.86%). In conclusion, presence of 150 mM alpha-tocopherol was the optimal concentration that is able to maintain the oocyte membrane stability post-cryopreservation.

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Sebuah penelitian telah dilakukan dengan menambahkan antioksidan glutathione pada pematangan sedang oosit domba garut. Penelitian dilakukan untuk mengevaluasi penambahan glutathione antioksidan dalam medium pematangan hingga kualitas oosit hingga postavitrifikasi. Sebanyak 129 oosit A ​​dan B berkualitas matang di dalam in vitro Media TCM-199 ditambahkan dengan antioksidan glutathione dengan konsentrasi 0 mM (KK); 0,5 mM (KP1); 1 mM (KP2); dan 1,5 mM (KP3). Oosit matang kemudian di vitrifikasi menggunakan kombinasi cryoprotectant 15% etilen glikol dan 15% dimetil sulfoksida. Evaluasi oosit dilakukan setelah 7 hari penyimpanan dalam cairan nitrogen (-196 ° C), termasuk kematangan oosit berdasarkan keberadaan benda polar, morfologi oosit, dan viabilitas oosit menggunakan pewarna Hoechst dan propidium iodide. Berdasarkan hasil penelitian, persentase yang diperoleh setelah postaturasi oosit matang adalah 0 mM (53,13%); 0,5 mM (55,88%); 1 mM (59,38%); dan 1,5 mM (67,74%). Persentase normal oosit pasca-vitrifikasi yaitu 0 mM (56,25%); 0,5 mM (64,71%); 1 mM (71,88%); dan 1,5 mM (87,10%). Persentase oocytrification hidup adalah 0 mM (56,25%); 0,5 mM (67,65%); 1 mM (71,88%); dan 1,5 mM (87,10%). Hasil uji statistik ANAVA data yang diperoleh tidak berbeda secara signifikan antara kelompok (P> 0,05), namun grafik Persentase menunjukkan pola yang cenderung meningkat dengan penambahan konsentrasi antioksidan glutathione. Kesimpulan dari penelitian berdasarkan hasil yang diperoleh yaitu penambahan antioksidan glutathione ke media pematangan dapat menjaga kualitas oosit ke post-trivirifikasi walaupun tidak signifikan.

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The study has been done by adding the antioxidant glutathione to the medium maturation of arrowroot sheep oocytes. The study was conducted to evaluate the addition antioxidant glutathione in the medium of maturation to oocyte quality up to postavitrification. A total of 129 quality A and B oocytes were matured in vitro inside TCM-199 medium added with antioxidant glutathione with a concentration of 0 mM (KK); 0.5 mM (KP1); 1 mM (KP2); and 1.5 mM (KP3). A mature oocyte then vitrified using a cryoprotectant combination of 15% ethylene glycol and 15% dimethyl sulfoxide. Oocyte evaluation is carried out after 7 days of storage in nitrogen liquid (-196 ° C), including oocyte maturity based on the presence of polar bodies, morphology oocytes, and oocyte viability using Hoechst and propidium iodide dyes. Based on the results of the study, the percentage obtained after postaturation of mature oocytes is 0 mM (53.13%); 0.5 mM (55.88%); 1 mM (59.38%); and 1.5 mM (67.74%). Percentage normal post-vitrified oocytes ie 0 mM (56.25%); 0.5 mM (64.71%); 1 mM (71.88%); and 1.5 mM (87.10%). The percentage of live oocytrification was 0 mM (56.25%); 0.5 mM (67.65%); 1 mM (71.88%); and 1.5 mM (87.10%). ANAVA statistical test results the data obtained did not differ significantly between groups (P> 0.05), however Percentage graphs show patterns that tend to increase with addition concentration of antioxidant glutathione. Conclusions of the study based on the results obtained namely the addition of glutathione antioxidants to the maturation medium can maintain the quality of the oocyte to the post-trivirification although not significant.