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"ABSTRAKLatar Belakang: Streptococcus mutans adalah bakteri penyebab karies dan memiliki faktor virulensi yang tinggi sehingga dapat memicu pembentukan biofilm pada gigi. Propolis merupakan bahan alami yang memiliki sifat antibakteri. Permen propolis hisap UI diduga dapat menghambat pembentukan biofilm bakteri Streptococcus mutans yang diisolasi dari plak gigi. Tujuan: Mengetahui efek permen propolis hisap UI terhadap pembentukan biofilm Streptococcus mutans yang diisolasi dari plak gigi. Metode: Streptococcus mutans pada sampel plak subjek diambil sebelum dan setelah perlakuan. Sampel dikultur dalam medium TYS20B kemudian dilakukan kultur pada medium cair TYS Broth. Setelah itu dilakukan uji biofilm dengan crystal violet assay. Hasil: Hasil uji Wilcoxon menyimpulkan tidak terdapat perbedaan pembentukan biofilm Streptococcus mutans yang bermakna sebelum dan setelah konsumsi permen propolis (p=0,697) Kesimpulan: Setelah konsumsi selama tujuh hari, permen propolis tidak mempengaruhi pembentukan biofilm Streptococcus mutans yang diisolasi dari plak gigi.

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ABSTRACTBackground: Streptococcus mutans is one of the etiology of caries with high virulence factors which can improve its ability to form biofilm on dental surface. Propolis is a natural product which has antibacterial properties. Propolis candy made by University of Indonesia was expected to decrease the ability of Streptococcus mutans to form biofilm. Aim: To know the effect of propolis candy on biofilm formation ability of Streptococcus mutans isolated from dental plaque. Method: Streptococcus mutans specimen from dental plaque of 120 subjects were collected pre and post treatment. Plaque sample then cultured on agar medium TYS20B and then cultured on TYS Broth medium. Then, biofilm formation of Streptococcus mutans from sample evaluated by crystal violet assay. Result: The results of Wilcoxon test conclude that there is no statistically significant difference between the biofilm formation of Streptococcus mutans before and after consumption of propolis candy (p=0,697) Conclusion: After seven days of consumption, propolis candy has no effect on biofilm formation of Streptococcus mutans isolated from dental plaque."

"Latar Belakang: Silver Diamine Fluoride SDF merupakan agen antikaries yang sudah terbukti efektif. Namun Silver Diamine Fluoride dapat menyebabkan noda hitam pada permukaan gigi dan menghasilkan rasa metal. Propolis Fluoride merupakan agen antibakteri yang dapat mencegah karies tanpa menyebabkan noda hitam dan rasa metal. Tujuan: tujuan dari penelitian ini untuk mengetahui keefektifan dari Propolis Fluoride dalam menghambat pembentukan biofilm S.mutans dan S.sanguinis dan dibandingkan dengan Silver Diamine Fluoride. Metode: suspensi bakteri dikultur pada Brain Heart Infusion Broth dan agar diteteskan kedalam 96-well plates dan dipaparkan dengan Propolis fluoride lalu diinkubasi pada waktu 4 jam, 12 jam, 24 jam, dan 48 jam. Selanjutnya dipaparkan Kristal violet 0,5 pada masing-masing well plate dan dilakukan evaluasi dengan menggunakan microplate reader untuk mengetahui nilai Optical Density dengan panjang gelombang 570nm. Hasil: Propolis Fluoride efektif dalam menghambat pembentukan biofilm S.mutans dan S.sanguinis. hasil uji ANOVA dari Potensi hambat pembentukan biofilm S.mutans dan S.sanguinis pada Propolis Fluoride setara dengan Silver Diamine Fluoride 38. Kesimpulan: Propolis Fluoride memiliki daya hambat dan keefektifan yang sama dengan Silver Diamine Fluoride.Kata Kunci: Propolis Fluoride, S. mutans, S.sanguinis, Kristal violet, Silver Diamine Fluoride. ......Silver Diamine Fluoride SDF is an anti caries agent that has been proven effective. But Silver Diamine Fluoride can caused black stain on the surface of the teeth and has a metallic taste. Propolis Fluoride is an antibacterial agent that can prevent caries without causing black stain on the surface of the teeth and metallic taste. Objective: the aim of this study is to know the effectiveness of Propolis Fluoride to inhibit the biofilm formation of S.mutans and S.sanguinis in comparation to Silver Diamine Fluoride. Methods: The suspension of the bacteria were cultured in Brain Heart Infusion Broth and agar were shed on 96 well plates and combined with Propolis fluoride then incubated for 4 hours, 12 hours, 24 hours, and 48 hours. After that, Cristal Violet 0,5 were shed on each well plate and evaluated using microplate reader to know the amount of Optical Density in 570nm wavelength. Result: Propolis Fluoride is effective for inhibiting biofilm formation of S.mutans and S.sanguinis. The ANOVA result of potential to inhibit biofilm formation of S.mutans and S.sanguinis for Propolis Fluoride is equal to Silver Diamine Fluoride 38. Conclusion The effectiveness and inhibition potential of biofilm formation using Propolis Fluoride is equal to Silver Diamine Fluoride. "