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Abstrak :
Essential oil mampu membunuh bakteri penghasil VSCs penyebab bau mulut sehingga penelitian ini ingin menguji efektivitas strip dari edible-film mengandung lima macam essential oil terhadap bau mulut. Penelitian ini adalah uji klinis menggunakan desain cross-over, randomized-double-blind, dan placebo-controlled-trial dengan 15 partisipan laki-laki. Parameter yang diukur adalah tongue coating, skor organoleptik, dan VSCs. Berdasarkan hasil penelitian, strip essential oil tidak efektif menurunkan tongue coating, namun, terbukti efektif menurunkan skor organoleptik hingga 60 menit dan menurunkan VSCs hingga 30 menit. Placebo tidak efektif menurunkan ketiga parameter bau mulut. Oleh karena itu, strip essential oil lebih efektif menurunkan bau mulut dibandingkan dengan placebo.

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Essential oil could kill the VSCs-producing bacteria, so, this research was to determine the efficacy of strip made from edible-film containing five types of essential oil on oral malodor. This research was clinical trial using cross-over, randomized-double-blind, and placebo-controlled-trial design with 15 male participants. The measured parameters were tongue coating, organoleptic score, and VSCs. From the result, essential oil strip couldn`t reduce tongue coating, however, it was significantly reduce organoleptic score until 60 minutes and VSCs until 30 minutes. Placebo couldn`t reduce all three oral malodor parameters. So, essential oil strip is more effective than placebo in reducing oral malodor.

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ABSTRAK Pendahuluan: Meningkatnya jumlah pasien dewasa dengan restorasi sewarna gigi, seperti resin komposit, menyebabkan perekatan braket pada permukaan artifisial gigi menjadi suatu tantangan tersendiri karena sering terjadi kegagalan rekat. Saat ini, belum ada riset yang dilakukan untuk melihat perbedaan nilai kekuatan rekat braket (baik nilai kuat geser maupun nilai kuat tarik braket) antara pada permukaan email gigi dan resin komposit nanohibrid. Metode: 32 gigi premolar bawah dibagi menjadi 4 kelompok: kelompok A1) spesimen email gigi (uji kuat rekat geser); kelompok A2) spesimen resin komposit nanohibrid (uji kuat rekat geser); kelompok B1) spesimen email gigi (uji kuat rekat tarik); kelompok B2) spesimen resin komposit nanohibrid (uji kuat rekat tarik). Braket direkatkan pada spesimen lalu diuji dengan menggunakan Universal Testing Machine Shimazu AG-5000 dalam waktu 24 jam setelah braket direkatkan. Hasil: Rerata nilai kuat rekat geser pada kelompok A1 sebesar 10.78 ± 0.13 MPa dan pada kelompok A2 sebesar 10.63 ± 0.18 MPa. Rerata nilai kuat rekat tarik pada kelompok B1 sebesar 10.74 ± 0.15 MPa dan pada kelompok B2 sebesar 10.65 ± 0.14 MPa. Pada uji statistik tidak terdapat perbedaan bermakna secara pada nilai kuat rekat geser maupun nilai kuat rekat tarik braket metal antara pada permukaan email gigi dan resin komposit nanohibrid. Kesimpulan: Tidak terdapat perbedaan bermakna secara statistik pada nilai kuat rekat geser maupun nilai kuat rekat tarik braket metal antara pada permukaan email gigi dan resin komposit nanohibrid. Seluruh kelompok memiliki nilai rerata kuat rekat geser dan nilai rerata kuat rekat tarik yang memadai untuk keperluan klinis perawatan ortodontik.

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ABSTRACT Introduction: The increasing number of adult patients with tooth-colored restorations, such as composite resins, causes the attachment of brackets on artificial surfaces of teeth to be a challenge because of frequent adhesive failures. At present, no research has been carried out to see the difference in bracket adhesive strength values (both shear bond strength and tensile bond strength) between the enamel surface and nanohybrid composite resins surface. Methods: 32 lower premolar were divided into 4 groups: group A1) dental enamel specimens for shear bond strength test; group A2) nanohybrid composite resins specimens for shear bond strength test; group B1) dental enamel specimens for tensile bond strength test; group B2) nanohybrid composite resins specimens for tensile bond strength test. The bracket was bonded to the specimens and tested using Universal Testing Machine Shimazu AG-5000 within 24 hours after the bracket was bonded to the specimens. Results: The mean value of shear bond strength in group A1 was 10.78 ± 0.13 MPa and in group A2 was 10.63 ± 0.18 MPa. The mean value of tensile bond strength in group B1 was 10.74 ± 0.15 MPa and in group B2 was 10.65 ± 0.14 MPa. In the statistical test there was no significant difference in the shear bond strength value or the tensile bond strength value between the metal bracket bonded to the enamel surfaces and to the nanohybrid composite resin surfaces. Conclusion: There was no significant difference in the shear bond strength and the tensile bonding strength value of the metal bracket bonded to the enamel surface and the nanohybrid composite resin surfaces. All groups have an adequate bond strength value for the clinical needs of orthodontic treatment.

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Latar belakang : Konsep balanced occlusion umum digunakan pada pembuatan GTP, namun konsep canine guidance menawarkan proses yang sederhana dan mengurangi resorbsi alveolar ridge. Diperlukan penelitian mengenai perbandingan kedua konsep oklusi ini di Indonesia. Tujuan : Menganalisis efektivitas konsep canine guidance dibandingkan balanced occlusion pada pemakai gigi tiruan penuh (GTP). Metode : Sepuluh pemakai GTP berpartisipasi dalam uji klinis dengan desain menyilang, lima subjek dipilih acak untuk memakai GTP balanced occlusion kemudian canine guidance dan lima yang lain memakai GTP canine guidance kemudian balanced occlusion. Pengukuran dilakukan tiga puluh hari setelah pemakaian setiap konsep oklusi. Aktivitas elektromiograf otot masseter superfisialis dan temporalis anterior subjek direkam dengan alat EMG, dan subjek mengisi kuesioner kemampuan mastikasi. Hasil : Rerata antara aktivitas EMG otot masseter superfisialis dan otot temporalis anterior pada pemakaian GTP canine guidance berbeda bermakna (p < 0,05) dengan balanced occlusion. Rerata antara skor kuesioner kemampuan mastikasi pada pemakaian GTP canine guidance berbeda bermakna (p=0,046) dengan balanced occlusion. Terdapat korelasi yang bermakna (p = 0,045) dan kuat (r=0,642) antara aktivitas EMG otot temporalis anterior dengan skor kuesioner kemampuan mastikasi saat pemakaian GTP balanced occlusion dan antara aktivitas EMG otot masseter superfisialis dengan skor kuesioner kemampuan mastikasi (p=0,045 ; r=0,648) saat pemakaian GTP canine guidance. Kesimpulan : Aktivitas otot mastikasi saat memakai GTP canine guidance lebih rendah signifikan secara klinis dan berbeda bermakna secara statistik dengan balanced occlusion. Kemampuan mastikasi secara subjektif juga lebih baik saat memakai GTP canine guidance.

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Background : Balanced occlusion commonly used in complete denture fabrication, however, canine guidance offers a simple process and reduce alveolar ridge resorption. Comparative study of these two concepts occlusion is required in Indonesia. Objective: To analyze the effectiveness of canine guidance to the balanced occlusion in complete denture wearers. Methods: Ten denture wearers participating in cross-over clinical trials, five subject randomly selected to wear balanced occlusion followed by canine guidance, five others wearing canine guidance followed by balanced occlusion. Outcomes were measured after 30 days of each occlusal scheme. Elektromiograf activities of superficial masseter muscle and anterior temporal muscle were recorded, participants also answered a masticatory ability questionnaire. Results: There are significant differences between the EMG activity of superficial masseter muscle and the anterior temporal muscle canine guidance on canine guidance wearers and balance occlusion (p <0.05). Patients rated their masticatory ability significantly better for the canine guidance denture (p = 0.046). There are significant and strong correlation (p = 0.045 ; r = 0.642) between the EMG activity of anterior temporal muscle and masticatory ability upon wearing balanced occlusion denture, and also between the superficial EMG activity of superficial masseter muscle and masticatory ability (p = 0.043; r = 0.648) upon wearing canine guidance denture. Conclusion: EMG activity of masticatory muscles upon wearing canine guidance denture are clinically and statistically lower to balanced occlusion. Masticatory ability are also better when using canine guidance denture according to the patients.

Abstrak :
Latar Belakang: Salah satu tujuan dalam keikutsertaan Indonesia pada program pembangunan global berkelanjutan (SDGS) yaitu meningkatkan status kesehatan. Upaya tersebut tidak terlepas dari berbagai faktor pendukung seperti ketersediaan sumber daya tenaga medis, kemudahan akses manusia terhadap fasilitas kesehatan, sehingga manusia dengan mudah berkunjung dan mendapatkan perawatan di fasilitas kesehatan. Tujuan : Mengetahui hubungan antara faktor sosiodemografi dan pelayanan kesehatan tooth decay dan filling di Indonesia. Metode: Penelitian cross sectional menggunakan data sekunder Riskesdas 2018 yang diklasifikasikan berdasarkan umur WHO sebanyak 14.031 mengenai variabel karakteristik sosidemografi (umur, jenis kelamin, tempat tinggal, status pekerjaan, dan tingkat pendidikan), utilisasi frekuensi kunjungan dan total decay serta filling. Pada Rifaskes 2019 menggunakan data sebanyak 17.741 fasilitas kesehatan. Jumlah dokter gigi menggunakan data Konsil Kedokteran Indonesia hingga bulan Desember 2021 sebanyak 22.926 dokter gigi. Variabel-variabel tersebut di uji secara statistik kemudian dipetakan menggunakan QGIS. Hasil: Uji Mann-Whitney menunjukkan terdapat perbedaan bermakna (p<0,05) antara kelompok jenis kelamin, lokasi tempat tinggal dan pencarian pengobatan terhadap tooth decay dan filling. Uji Kruskal-Wallis menunjukkan terdapat perbedaan bermakna (p<0,05) antara kelompok status pekerjaan, tingkat pendidikan, dan utilisasi frekuensi kunjungan. Sedangkan Uji korelasi Spearman menunjukkan terdapat korelasi antara jumlah dan rasio fasilitas kesehatan serta dokter gigi terhadap tooth decay dan filling. Kesimpulan: Pada penelitian ini terdapat hubungan antara faktor sosidemografi dan pelayanan kesehatan yang memengaruhi tooth decay dan filling. Upaya pemerataan distribusi fasilitas kesehatan dan dokter gigi, serta intervensi dalam meningkatkan utilisasi dengan melihat aspek karakteristik sosiodemografi. ......Backgorund: One of the goals of Indonesia's participation on sustainable development goals is improving health state. Efforts made to achieve health improvement is increasing availability of health care facilities so people can easily access and get treatment for dental and oral health. Objective: This study aimed to determine relationship between sociodemographic and health services factors that affect tooth decay and filling in Indonesia. Methods: A cross sectional study using secondary data from Riskesdas 2018 as classified based WHO age as much 14.031 subject are sociodemography factors, utilization dental visit, total decay and filling. Rifaskes 2019 data’s using 17.741 healthcare facilities. Number of dentist as much 22.926 using data Indonesia Medical Council on December 2021. These variabels were tested statistically then mapping using QGIS. Results: Mann-Whitney test showed a significant difference (p<0,05) between groups of gender, place of residence towards tooth decay and filling. Kruskal-Wallis test showed a significant difference (p<0,05) between groups of occupational status, education level, and frequency utilization towards tooth decay and filling. Spearman test showed a correlation statistically (p<0,05) between amount and ratio of healthcare facilities and dentist towards tooth decay and filling. Conclusion: In this study there are relationship between sociodemographic factors and healthcare services that affect tooth decay and filling. Efforts to equitable distribution of healthcare facilities and dentist, as well as intervention to increase utilization by looking all aspect of sociodemographic characteristics.

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ABSTRAK
Penelitian ini ditujukan untuk mengetahui dan membandingkan pengaruh pasta gigi yang mengandung theobromine dan hydroxyapatite terhadap kekerasan mikro email gigi setelah direndam dalam minuman berkarbonasi. Penelitian ini menggunakan 21 spesimen mahkota gigi premolar satu rahang atas. Spesimen direndam dalam minuman berkarbonasi kemudian disikat dengan pasta gigi yang mengandung theobromine dan hydroxyapatite. Uji Kruskal-Wallis dan Mann- Whitney menunjukkan perubahan kekerasan yang signifikan setelah penyikatan selama 9 menit 20 detik menggunakan kedua pasta gigi pasca direndam dalam minuman berkarbonasi selama 10 menit. Peningkatan nilai kekerasan mikro email gigi pada kelompok hydroxyapatite lebih besar dibandingkan kelompok theobromine (p<0,05).

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ABSTRACT
This study is aimed to analyze the effect of theobromine and hydroxyapatite toothpaste to enamel microhardess after immersion in carbonated drink. This study uses 21 upper first premolar crown specimen which is immersed in carbonated drink then brushed with theobromine and hydroxyapatite containing toothpaste. Kruskal-Wallis and Mann-Whitney test shows significant difference of microhardness after brushing for 9 minutes and 20 seconds using both toothpastes after immersion in carbonated drink for 10 minutes with higher number in hydroxyapatite group than theobromine group (p<0,05).

Abstrak :
Latar Belakang: Prevalensi karies gigi di Indonesia 90% (2010). "Suku Anak Dalam" di pedalaman hutan Provinsi Jambi menganut paham animisme yang mempunyai pantangan menggunakan pasta gigi. Tujuan Penelitian: Mengidentifikasi pengetahuan, sikap, dan tindakan murid "Sokola Rimba" serta kaitannya dengan kepercayaan memelihara kesehatan gigi dan mulut terhadap risiko karies gigi. Metode: Deskriptif kualitatif etnografi dan kuantitatif cross-sectional dengan metode convenience sampling. Hasil Penelitian: Status karies gigi menurut indeks deft anak usia 5-10 tahun 5,18; indeks DMFT anak usia 5-10 tahun 4,59 dan remaja usia 12-18 tahun 16,53. Kesimpulan: Status karies gigi berkaitan dengan perilaku dan kepercayaan masyarakat "Suku Anak Dalam". ......Background: Prevalence of caries in Indonesia is 90% (2010). "Suku Anak Dalam" in the jungle of Jambi Province believed in animism which prohibits the use of toothpaste. Objective: To identify the knowledge, attitudes, and practice "Sokola Rimba" students with its relation to the belief of maintaining dental and oral health on the risk of dental caries. Methods: Descriptive qualitative ethnographic and quantitative cross-sectional with convenience sampling method. Result: Caries status according to def-t index 5,18 children 5-10 years of age; DMF-T index of 4,59 children aged 5-10 years and adolescents aged 12-18 years are 16,53. Caries severity is related to the behavior and beliefs in "Suku Anak Dalam".

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ABSTRACT
Penelitian terkait infeksi odontogenik di Indonesia masih sangat sedikit. Penelitian ini dilakukan untuk mengetahui profil infeksi odontogenik di Rumah Sakit Umum Pusat Nasional Dr. Cipto Mangunkusumo periode 1 Januari 2015 ndash; 31 Desember 2015. Penelitian ini merupakan studi deskriptif retrospektif dengan menggunakan data sekunder yang didapat dari rekam medik Rumah Sakit Umum Pusat Nasional Dr. Cipto Mangunkusumo. Analisis dilakukan pada 61 rekam medik kasus infeksi odontogenik. Profil infeksi odontogenik dilihat berdasarkan usia, jenis kelamin, dan elemen gigi penyebab. Mayoritas pasien berusia 52-61 tahun 19,67 dan berjenis kelamin perempuan 56 . Elemen gigi penyebab infeksi odontogenik terbanyak adalah gigi molar mandibular pertama kiri 16.

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ABSTRACT
Study about number of odontogenic infections in Indonesia is rarely done. This research was aimed to know the profile of odontogenic infections in Dr. Ciptomangunkusumo General Hospital Patients on January 2015 ndash December 2015. This research was a descriptive retrospective study from the medical records of Dr. Ciptomangunkusumo General Hospital Patients. 61 medical records were analyzed. Profile of odontogenic infections was analyzed concerning age, gender, and primary site of odontogenic infections. The majority of patients are in 52 61 age group 19,67 , and female were more involved than male 56 . The primary site of odontogenic infection is the first left mandibular molar 16.

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Latar Belakang: Perawatan untuk perubahan warna gigi yang banyak dipilih masyarakat saat ini ialah tooth bleaching atau pemutihan gigi. Teknik home bleaching kerap menjadi pilihan masyarakat karena lebih murah serta tidak menimbulkan efek hipersensitivitas yang tinggi. Penggunaan bahan alami seperti buah-buahan dapat dimanfaatkan pada bidang kesehatan dan kecantikan termasuk untuk tooth bleaching. Buah tomat dapat dimanfaatkan sebagai agen pemutih tambahan untuk produk home bleaching karena mengandung agen pengoksidasi yang dapat mempercepat proses pemutihan gigi. Tujuan: Membuat material home bleaching hidrogen peroksida 3% dengan penambahan jus buah tomat dan mengetahui perbedaan warna gigi setelah aplikasi bahan bleaching. Metode: Dua puluh empat gigi premolar pasca ekstrasi diberi paparan bahan home bleaching. Sampel dibagi menjadi 4 kelompok dengan masing-masing terdiri dari 6 sampel. Kelompok A dipaparkan bahan bleaching hidrogen peroksida 3%, kelompok B hidrogen peroksida 3% dengan tambahan jus tomat 30%, kelompok C hidrogen peroksida 3% dengan tambahan jus tomat 75%, dan kelompok D dipaparkan bahan home bleaching komersial opalescence whitening gel PF 10%. Setiap kelompok dipaparkan 8 jam/hari selama 7 hari. Perubahan warna diukur sebelum dan sesudah paparan menggunakan kolorimeter dengan metode CIEL*a*b. Analisis data dengan uji statistik One-Way ANOVA dan Post Hoc Bonferroni. Hasil: Penelitian menunjukkan bahwa keempat kelompok dapat memutihkan gigi. Hasil perubahan warna ∆E*ab kelompok dengan hidrogen peroksida 3% 5,89, hasil ∆E*ab kelompok hidrogen peroksida 3% dengan tambahan jus tomat 30% 27,93, hasil ∆E*ab kelompok hidrogen peroksida 3% dengan tambahan jus tomat 75% 23,27, hasil ∆E*ab kelompok opalescence whitening gel PF 10% 10,67. Hasil ∆E*ab penambahan jus tomat lebih tinggi dibandingkan bahan hidrogen peroksida 3% dan bahan home bleaching komersial opalescence whitening gel PF 10%. Hasil ∆E*ab setiap kelompok terdapat perbedaan bermakna (p<0,05). Kesimpulan: Terdapat pengaruh penambahan jus buah tomat pada bahan bleaching hidrogen peroksida 3% terhadap perubahan warna gigi yang lebih cerah. ......Background: Tooth bleaching is one of the treatment that many people choose in the management for tooth discoloration. Home bleaching technique often chosen because cheaper and do not cause high hypersensitivity effects. The use of natural ingredients such as fruits can be utilized in the health and beauty sector, including for tooth bleaching. Fruits, such as tomato can be used as an additional whitening agent for home bleaching products because it contains an oxidizing agent which can speed up the teeth whitening process. Objective: To make 3% hydrogen peroxide bleaching at home with the addition of tomato juice and find the difference in tooth colour after application of the bleaching agent. Methods: Twenty four post-extraction premolars were exposed to home bleaching agents. The sample was divided into 4 groups with 6 samples each. Group A was exposed to 3% hydrogen peroxide bleaching agent, group B was exposed to 3% hydrogen peroxide with the addition of 30% tomato juice, group C was exposed to 3% hydrogen peroxide with the addition of 75% tomato juice, and group D was exposed to commercial home bleaching agent opalescence whitening gel PF 10% . Each group was exposed 8 hours/day for 7 days. Colour changes were measured before and after exposure using a colorimeter with the CIEL*a*b method. Data analysis with One-Way ANOVA and Post Hoc Bonferroni statistical tests. Results: Research shows that all four groups can whiten teeth. Color change results in hydrogen peroksida 3% ∆E*ab 5,89, ∆E*ab % hydrogen peroxide with the addition of 30% tomato juice 27,93, ∆E*ab % hydrogen peroxide with the addition of 75% tomato juice 23,27, and ∆E*ab opalescence whitening gel PF 10% 10,67. The results of the discoloration of the ∆E*ab group with 3% hydrogen peroxide and the addition of tomato juice were higher than those of 3% hydrogen peroxide without addition of tomato juice and exposed to commercial home bleaching agents, opalescence whitening gel PF 10%. Color change results between groups significantly different (p<0,05). Conclusion: There is an effect of adding tomato juice to 3% hydrogen peroxide bleaching agent on teeth discoloration that is brighter.

Abstrak :
Latar Belakang: Setiap tahap odontogenesis yang tidak berjalan dengan baik semestinya dapat mengakibatkan terjadinya abnormalitas pada gigi, hal tersebut dipengaruhi oleh beberapa faktor atau komponen tertentu seperti gen, nutrisi, mineral, molekul, atau lainnya. Untuk memahami proses terjadinya abnormalitas serta faktor-faktor yang memengaruhinya, perlu diketahui secara spesifik setiap tahapan perkembangan gigi. Penelitian terkait perkembangan gigi pada manusia tidak memungkinkan karena diperlukan tindakan yang invasif dalam pengambilan sampel jaringan. Oleh sebab itu, digunakan hewan coba mencit C57BL/6 karena merupakan jenis inbred dan banyak digunakan dalam penelitian biomolekuler. Namun, hingga saat ini penelitian mengenai odontogenesis pada mencit C57BL/6 masih sangat terbatas. Tujuan: Menganalisis perkembangan gigi (odontogenesis) mencit strain C57BL/6 usia satu hari. Metode: Rahang mencit C57BL/6 dipotong menjadi 4 bagian. Setelah itu, dilakukan pembuatan preparat dengan potongan longitudinal (sagital) pada maksila dan mandibula kanan dan potongan koronal (frontal) pada maksila dan mandibula kiri, serta dilakukan pewarnaan hematoksilin dan eosin (H&E) pada preparat dan dilakukan pengamatan benih gigi insisif dan molar rahang atas dan rahang bawah menggunakan mikroskop. Hasil: Terlihat benih gigi insisif, molar pertama, molar kedua, dan molar ketiga pada jaringan maksila dengan potongan transversal atau axial dan terlihat adanya benih gigi molar pada preparat jaringan maksila dan mandibula dengan potongan koronal. Pada jaringan mandibula dengan potongan longitudinal atau sagital hanya terlihat adanya benih gigi insisif. Kesimpulan: Terjadi proses odontogenesis hingga tahap awal bell stage pada gigi molar maksila kiri dan mandibula kiri mencit C57BL/6 usia satu hari, sehingga mencit C57BL/6 usia satu hari dapat dijadikan alternatif objek penelitian dalam menganalisis perkembangan struktur jaringan gigi baik pada kondisi normal maupun patologis. ......Background: Disruption in the stages of odontogenesis results in teeth abnormalities. These conditions can be influenced by certain factors or components such as genes, nutrients, minerals, molecules, or others. To understand the process of abnormality and the factors that influence it, it is necessary to know specifically each stage of tooth development. Research related to the development of teeth in humans is not possible because it requires an invasive procedure in tissue sampling. Therefore, C57BL/6 mice were used as experimental animals because they are an inbred species and are widely used in biomolecular research. However, until now research on odontogenesis in C57BL/6 mice is still very limited. Objective: Analyzing tooth development (odontogenesis) in one-day-old C57BL/6 mice. Methods: The jaws of C57BL/6 mice were cut into 4 parts. After that, preparations were made with longitudinal (sagittal) sections on the right maxilla and mandible and coronal (frontal) sections on the left maxilla and mandible, and stained with hematoxylin and eosin (H&E) on the preparations and observation of the maxillary and mandibular incisors and molars using a microscope. Results: Tooth germs of incisors, first molars, second molars, and third molars were observed in the maxillary tissue with transverse or axial sections and the presence of molars in the maxillary and mandibular tissue preparations with coronal sections. In mandibular tissue with longitudinal or sagittal sections, only incisor germs were seen. Conclusion: The odontogenesis of one-day-old C57BL/6 mice in this study were observed in the early bell stage both in the left maxillary and left mandibular molars. Based on this study, one-day-old C57BL/6 mice can be used as an alternative object of research in analyzing the tooth structure that have been developed in the early bell stage, both normal and pathological conditions.

Abstrak :
ABSTRAK
Latar Belakang: Berbagai material kaping pulpa berbahan dasar kalsium silikat terus dikembangkan, diantaranya semen berbasis kalsium silikat dengan penambahan steroid. Tujuan: Menganalisis efek penambahan steroid pada semen berbasis kalsium silikat terhadap viabilitas sel fibroblas. Metode: Sel fibroblas embrio ayam direndam dalam ekstrak larutan semen berbasis kalsium silikat dengan penambahan steroid dan MTA. Viabilitas sel dihitung dengan menggunakan uji MTT. Hasil: Terdapat perbedaan bermakna (p ≤ 0,05) viabilitas sel pada kelompok semen berbasis kalsium silikat dengan penambahan steroid dibandingkan kelompok kontrol dan MTA. Kesimpulan: Penambahan steroid menurunkan viabilitas sel. Terdapat peningkatan pada 72 jam, yang menandakan terjadinya proliferasi sel.ABSTRACT
Background: Calcium silicate based materials are being developed continuously, one of them is calcium silicate based cement containing steroid. Objective: To analyze the effect of steroid addition in calcium silicate based cement on fibroblast cells viability. Methods: Chicken embryonic fibroblast cells were immersed in extract solution of calcium silicate based cement containing steroid and MTA. Viability was analyzed by MTT Assay. Results: Significant difference (p ≤ 0,05) of viability on calcium silicate based cement containing steroid group was found, compared to control and MTA group. Conclusion: Steroid addition decrease viability. There was an increase in 72 hours, marking cells proliferation.;Background: Calcium silicate based materials are being developed continuously, one of them is calcium silicate based cement containing steroid. Objective: To analyze the effect of steroid addition in calcium silicate based cement on fibroblast cells viability. Methods: Chicken embryonic fibroblast cells were immersed in extract solution of calcium silicate based cement containing steroid and MTA. Viability was analyzed by MTT Assay. Results: Significant difference (p ≤ 0,05) of viability on calcium silicate based cement containing steroid group was found, compared to control and MTA group. Conclusion: Steroid addition decrease viability. There was an increase in 72 hours, marking cells proliferation.;Background: Calcium silicate based materials are being developed continuously, one of them is calcium silicate based cement containing steroid. Objective: To analyze the effect of steroid addition in calcium silicate based cement on fibroblast cells viability. Methods: Chicken embryonic fibroblast cells were immersed in extract solution of calcium silicate based cement containing steroid and MTA. Viability was analyzed by MTT Assay. Results: Significant difference (p ≤ 0,05) of viability on calcium silicate based cement containing steroid group was found, compared to control and MTA group. Conclusion: Steroid addition decrease viability. There was an increase in 72 hours, marking cells proliferation.