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"Gen tilapia Growth Hormone (tiGH) merupakan gen pengkode hormon pertumbuhan dari ikan nila yang berperan untuk meningkatkan pertumbuhan. Penelitian bertujuan melakukan kloning dan ekspresi gen tiGH untuk memproduksi protein rekombinan hormon pertumbuhan ikan nila (Oreochromis niloticus). Penelitian meliputi tahapan isolasi gen tiGH dari pMBA_tiGH, ligasi ke dalam pETBlue-2, serta transformasi vektor rekombinan ke dalam sel inang dengan menggunakan elektroporasi. Hasil penelitian menunjukkan bahwa vektor rekombinan dapat ditransformasi ke dalam sel inang E. coli BL2 dengan efisiensi transformasi 1,12x103 cfu/μg. Ekspresi gen tiGH dilakukan menggunakan induksi IPTG 0,4 mM dan dipurifikasi menjadi protein rekombinan growth hormone dengan berat molekul sebesar 22 kDa.

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Tilapia growth hormone gene (tiGH) is a gene encoding growth hormone from the tilapia whose folr is to increase the growth. The research objective is to do cloning and expression tiGH gene to produce growth hormone recombinant proteins of tilapia (Oreochromis niloticus). Stages of research include isolation tiGH gene from pMBA_tiGH, ligation into pETBlue-2, and the transformation recombinant vector into host cells by using electroporation.

The result showed that recombinant vectors have been successfully transformed into the host cell E.coli BL21with transformation efficiency reached 1.12 x103 cfu/μg. Expression tiGH gene performed using 0.4 mM IPTG induction and purified recombinant protein growth hormone with a molecular weight of 22 kDa."

"Indonesian Journal of Dentistry 2006; Edisi Khusus KPPIKG XIV: 325-328Music is related to stress reduction and increased levels of growth-hormone. In rat, music exposure since prenatal period was found to increase body-weight of 7-days-old litters and brain cells. Somatic growth was primarily influenced by growth-hormone and supported by psycho-physic condition. The objective of this study was to analyze whether music exposure since prenatal until 35 days post-natal period could affect the weight and body-length of the rat-pups. Four pregnant Wistar rats were daily exposed to one hour classic music (Mozart) every 17.30 PM since gestation period day one until the 22 litters were 35-days-old. Controls were 5 pregnat rats and their 36 litters caged in a different room with no music. Weighing and measuring the body-length (the most anterior point of nasal-septum to the base of the tail) were conducted at day 7,25 and 35. Data were analyzed using Multivariate General-Linear-Model (a = 0.05). It was revealed that the mean weight of the experimental litters was significantly higher than those of the controls either at day 7 (p = 0.00), day 25 (p = 0.012). Conclusion: Music exposure since prenatal period has significant influences on the weight of the rat-litters aged 7,25 and 35 days and on the body-length of the 25-days-old litters."