Hasil Pencarian  ::  Simpan CSV :: Kembali

"Exfoliative cheilits is a rare chronic superficial inflammation disorder which affect vermilion border of the lips, especially lower lip. This disorder mostly happens in woman. The characteristics of this disease are a large amount production and desquamation of the keratin layers. The etiology and pathogenesis of exfoliative cheilitis is unknown. Diagnosis of exfoliative cheilitis can only be established if the conditions of the disease couldn't be connected to others factors , exfoliative cheilitis is very resistant to many treatment modalities which gave a difficulty to determine a therapy with an optimum result. Topical steroid was the most effective therapy for the disease. Antifungal therapy can be used only if there is a secondarily infection caused by Candida. This case report is about an exfoliative cheilitis on 42 years- old women with unknown etiology background. On intra-oral examination we found out that there were several focus of infections that can delay the healing proccess. The patient's conditions wereresolved after a few month of topical steroid therapy, a month of antifungal therapy and a management of the focus of infections. An exact diagnosis, an appropriate management, good corporation between sections which are related to patient condition and patient corporation are very important on the healing of exfoliative cheilitis."

"Tingginya konsumsi ayam meningkatkan pembuangan limbah bulu ayam, yang mengandung sekitar 90% keratin, menyebabkan pencemaran lingkungan. Keratin, protein struktural utama dalam jaringan hewan, berfungsi untuk berbagai aplikasi biomedis dan memerlukan ekstraksi ramah lingkungan. Penelitian ini mengoptimasi ekstraksi keratin dari bulu ayam menggunakan cairan ionik 1-Butyl-3-Methylimidazolium Asetat ([Bmim]-Asetat) dengan bantuan ultrasonic bath. Hasil penelitian menunjukkan bahwa metode panas dengan waktu 120 menit menghasilkan yield keratin tertinggi sebesar 78.20% dibandingkan metode optimasi ultrasonik dan metode ultrasonik tanpa optimasi. Karakterisasi FTIR menunjukkan spektra konsisten dengan struktur keratin, dan uji Bradford menunjukkan konsentrasi protein tertinggi pada metode panas dengan 112.42 mg/mL dibandingkan metode ultrasonik 120 menit dan optimasi ultrasonik 120 menit yang menghasilkan 12.13 mg/mL dan 8.834 mg/mL masing-masing. Analisis SDS-PAGE memastikan keberadaan protein keratin pada sampel hasil ekstraksi dengan berat molekul yang terlihat sebesar 10 kDa. Kombinasi gelombang ultrasonik dan suhu 90 oC dapat meningkatkan efisiensi ekstraksi dan pemulihan keratin dari limbah bulu ayam, menjadikannya metode yang lebih efisien dan ramah lingkungan.

---

The high consumption of chicken increases the disposal of chicken feather waste, which contains about 90% keratin, leading to environmental pollution. Keratin, the main structural protein in animal tissues, serves various biomedical applications and requires environmentally friendly extraction methods. This study optimizes the extraction of keratin from chicken feathers using the ionic liquid 1-Butyl-3-Methylimidazolium Acetate ([Bmim]-Acetate) with the assistance of an ultrasonic bath. The results show that the heat method with a duration of 120 minutes produced the highest keratin yield of 78.20%, compared to the optimized ultrasonic method and the non-optimized ultrasonic method. FTIR characterization indicated spectra consistent with the structure of keratin, and the Bradford assay revealed the highest protein concentration in the heat method at 112.42 mg/mL, compared to 12.13 mg/mL and 8.834 mg/mL in the 120-minute ultrasonic and optimized ultrasonic methods, respectively. SDS-PAGE analysis confirmed the presence of keratin protein in the extracted samples with a visible molecular weight of 10 kDa. The combination of ultrasonic waves and a temperature of 90°C can enhance the efficiency of keratin extraction and recovery from chicken feather waste, making it a more efficient and environmentally friendly method."

"Material biopolimer konvensional memiliki kelemahan, seperti tidak responsif dan tidak memiliki bioaktivitas terhadap mikoorganisme sehingga memiliki keterbatasan pada penerapannya dalam proses antibakteri dan penyembuhan luka. Oleh karena itu, pengembangan material berbasis hidrogel yang mampu mengatasi kekurangan tersebut menjadi penting untuk menunjang proses penyembuhan luka yang lebih efektif. Pada penelitian ini, telah dikembangkan film hidrogel komposit berbasis karboksimetil selulosa (KMS) dan keratin dengan asam sitrat sebagai agen pengikat silang dan gliserol sebagai plasticizer melalui metode solution casting dengan memvariasikan rasio massa KMS dan keratin. Hasil karakterisasi FTIR menunjukkan keberhasilan pembentukan hidrogel komposit KMS/keratin dengan munculnya gugus ester dan amida sebagai hasil ikatan silang antar komponen. Uji swelling menunjukkan bahwa rasio swelling menurun seiring meningkatnya rasio keratin. Sementara itu, hasil uji swelling pada berbagai pH menunjukkan bahwa hidrogel bersifat responsif terhadap pH, ditandai dengan peningkatan rasio swelling seiring dengan kenaikan pH. Hidrogel dengan kandungan keratin terendah menunjukkan rasio swelling tertinggi pada ketiga pH yang diuji. Selain itu, uji antibakteri terhadap E. coli dan S. aureus menunjukkan bahwa penambahan keratin memberikan aktivitas antibakteri yang ditunjukkan oleh pembentukan zona hambat.

---

Conventional biopolymer materials have weaknesses, such as unresponsiveness and lack of bioactivity towards mycoorganisms, which limit their application in antibacterial and wound healing processes. Therefore, the development of hydrogel-based materials that could address these shortcomings became important to support a more effective wound healing process. In this study, composite hydrogel films based on carboxymethyl cellulose (CMC) and keratin were developed using citric acid as a crosslinking agent and glycerol as a plasticizer through the solution casting method, with variations in the CMC-to-keratin mass ratio. FTIR characterization confirmed the successful formation of CMC/keratin composite hydrogels, as evidenced by the appearance of ester and amide groups resulting from crosslinking between the components. Swelling tests showed that the swelling ratio decreased as the keratin ratio increased. Meanwhile, swelling tests at different pH levels demonstrated that the hydrogels exhibited pH-responsiveness, indicated by an increase in swelling ratio with increasing pH. The hydrogel with the lowest keratin content exhibited the highest swelling ratio at all three pH levels tested. In addition, antibacterial tests against E. coli and S. aureus revealed that the incorporation of keratin provided antibacterial activity, as indicated by the formation of inhibition zones."

"Industri pemotongan unggas menghasilkan limbah dalam jumlah besar yang kaya akan keratin, yaitu protein berserat yang menyusun sekitar 90% berat kering bulu ayam. Keratin memiliki potensi tinggi untuk dimanfaatkan sebagai biomaterial atau bahan baku produk bioteknologi, namun pemanfaatannya masih terbatas akibat rendahnya kelarutan dalam pelarut umum yang disebabkan oleh banyaknya ikatan disulfida, sehingga proses ekstraksinya menjadi lebih sulit. Pada penelitian ini dilakukan ekstraksi keratin dari bulu ayam menggunakan metode Ionic Liquid 1-butil-3-metilimidazolium asetat ([Bmim]Ac) - Microwave Assisted Extraction (MAE) dengan penambahan natrium sulfit (Na2SO3) dan dibandingkan dengan metode pemanasan konvensional berbasis hotplate. Hasil penelitian menunjukkan bahwa metode IL-MAE dan penambahan Na2SO3 menghasilkan yield keratin tertinggi, yakni sebesar 83,899% dengan berat molekul 10 kDa yang diperoleh melalui SDS-PAGE. Spektrum FTIR menunjukkan adanya pita serapan khas gugus amida dan C–S, yang mengindikasikan keberadaan keratin. Selain itu, keratin hasil ekstraksi menunjukkan stabilitas termal yang paling tinggi, dengan total penurunan massa hanya 1,236% sampai suhu 500°C. Hasil XRD memperlihatkan bahwa hasil keratin didominasi oleh β-sheet, yang ditandai oleh kemunculan puncak pada sekitar 22°.

---

The poultry processing industry generates a large amount of waste rich in keratin, a fibrous protein that makes up approximately 90% of the dry weight of chicken feathers. Keratin has high potential for utilization as a biomaterial or as a precursor for biotechnological applications. However, its application remains limited due to its low solubility in common solvents, caused by the abundance of disulfide bonds, which makes the extraction process more difficult. In this study, keratin was extracted from chicken feathers using the Ionic Liquid ([Bmim]Ac)–Microwave Assisted Extraction (IL-MAE) method with the addition of Na₂SO₃, and compared with the conventional heating method using a hotplate. The results showed that the IL-MAE method with Na₂SO₃ addition produced the highest keratin yield, reaching 83.899%, with a molecular weight of 10 kDa as determined by SDS-PAGE. The FTIR spectrum exhibited characteristic absorption bands corresponding to amide and C–S functional groups, indicating the presence of keratin. In addition, the extracted keratin demonstrated the highest thermal stability, with a total mass loss of only 1.236% up to 500 °C. XRD analysis revealed that the keratin structure was predominantly composed of β-sheets, as evidenced by a distinct diffraction peak around 22°."

"Tingginya kebutuhan industri unggas menghasilkan limbah signifikan berupa bulu yang dibuang secara tidak terkendali dan menyebabkan masalah lingkungan. Bulu ayam mengandung lebih dari 90% protein dimana komponen utamanya keratin, yang memiliki potensi tinggi untuk berbagai aplikasi. Penelitian ini bertujuan untuk mengoptimalkan metode ekstraksi keratin dari bulu ayam menggunakan Ionic Liquid 1-butil-3-metilimidazolium asetat ditambahkan natrium sulfit (Na2SO3) sebagai agen pereduksi serta dibantu ultrasonic bath (IL-UAE) untuk meningkatkan efisiensi proses ekstraksi. Hasil penelitian menunjukkan bahwa metode IL-UAE dengan penambahan Na2SO3 menghasilkan yield keratin tertinggi sebesar 81,39% dibandingkan metode lainnya. Karakterisasi FTIR menunjukkan puncak serapan khas amida serta puncak C-S yang tajam. Analisis SDS-PAGE mengidentifikasi pita protein pada rentang berat molekul 10 kDa yang memastikan keberadaan keratin. Uji TGA menunjukkan bahwa metode IL-UAE dengan penambahan Na2SO3 memiliki stabilitas termal terbaik dengan penurunan massa terkecil sebesar 1,92 %. Sementara itu, hasil XRD memperlihatkan struktur keratin dalam bentuk semi-kristalin dengan intensitas puncak β-sheet pada 19-23o yang tampak lebih dominan.

---

The increasing demand in the poultry industry has led to the generation of significant waste in the form of feathers, which are often disposed of uncontrollably and cause environmental problems. Chicken feathers contain 90% protein, with keratin as the main component, which holds great potential for various applications. This study aims to optimize the extraction method of keratin from chicken feathers using the Ionic Liquid 1-butyl-3-methylimidazoilium acetate, combined with sodium sulfite as a reducing agent assisted by an ultrasonic bath (IL-UAE) to improve extraction efficiency. The results showed that the IL-UAE method with Na2SO3 addition yielded the highest keratin extraction rate of 81,39% compared to other methods. FTIR characterization revealed a sharp distinctive amide absorption peaks and C-S stretching bands. SDS-PAGE analysis identified protein bands within the molecular weight range of 10 kDa, confirming the presence of keratin. TGA analysis demonstrated that this method also provided the best thermal stability, with the lowest mass loss of only 1.92%. Meanwhile, XRD results indicated that the extracted keratin had a semi-crystalline structure, with dominant β-sheet intensity observed at 19-23o."