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"Pendahuluan : Akhir-akhir ini penelitian terkait mikrobiom kulit manusia menjadi fokus di bidang dermatologi dan kosmetik karena mikrobiota kulit yang memiliki fungsi vital dalam menjaga homeostasis kulit. Sudah banyak laporan disbiosis mikrobiom yang berhubungan dengan beberapa kondisi kulit, baik patologis maupun nonpatologis, contohnya pada penuaan atau aging. Pada kulit menua terdapat perubahan struktural dan fungsional kulit yang menyebabkan perubahan habitat mikrobiom, sehingga terjadi perubahan komposisi mikrobiota. Hal tersebut dapat menyebabkan disbiosis, sehingga dapat pula menjadi faktor predisposisi dalam proses penuaan kulit. Tujuan Penelitian : Menilai korelasi antara mikrobiom kulit dengan parameter penuaan kulit wajah perempuan Indonesia dan juga mengetahui gambaran mikrobiom pada kulit dewasa muda, lansia perempuan Indonesia, serta menilai perbedaan shannon index serta relative abundance mikrobiom kulit antara perempuan dewasa muda dan lansia. Metodologi Penelitian : Penelitian ini merupakan penelitian observasional analitik dengan desain potong lintang. Pengambilan sampel dilakukan pada bulan Februari – Maret 2023 di Poliklinik Dermatologi dan Venereologi (DV) RSCM. Subjek penelitian berjumlah 48 orang yang terdiri dari 24 orang perempuan sehat usia dewasa muda (21–37 tahun) dan 24 orang lansia (60–76 tahun) yang melewati kriteria penerimaan dan penolakan. Subjek penelitian yang terpilih melakukan kunjungan ke Poliklinik DV RSCM, dan dilakukan anamnesis, pemeriksaan klinis, pemeriksaan wajah menggunakan skin analyzer JANUS™ III, serta pengambilan apusan kulit (swab) pada kedua pipi. Hasil sampel apusan kulit kemudian dilakukan ekstraksi DNA menggunakan DNeasy PowerSoil Kit™ dan dilakukan sekuensing pada region V3-V4 16s rRNA dengan alat Next Generation Sequencing (NGS), MiSeq Illumina™. Total didapatkan 39 sampel DNA yang dapat diidentifikasi oleh alat MiSeq Illumina™. Hasil Penelitian : Abundance filum firmicutes dan genera staphylococcus secara bermakna lebih besar pada kelompok lansia. Shannon index kelompok dewasa muda lebih tinggi daripada kelompok lansia namun tidak berbeda bermakna dan hanya berkorelasi lemah terhadap usia (P>0,05). Terdapat korelasi positif antara Staphylococcus dengan usia, serta Paracoccus dengan porfirin. Terdapat korelasi negatif antara Shannon index dengan pori-pori, dan Cutibacterium dengan porfirin (P≤0,05) Kesimpulan : Hasil penelitian akhir didapatkan dari 39 sampel apusan kulit yang berhasil diidentifikasi oleh alat NGS, oleh karena itu perlu dilakukan penelitian yang membandingkan metode pengambilan sampel mikrobiom kulit wajah untuk standarisasi penelitian selanjutnya dan perlu dilakukan penelitian dengan jumlah sampel yang lebih banyak serta multi-centered untuk mewakili daerah Indonesia di pedalaman dengan iklim dan lingkungan yang berbeda dengan masyarakat urban.

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Introduction: Recently research related to the human skin microbiome has become a focus in the fields of dermatology and cosmetics because skin microbiome has a vital function in maintaining skin homeostasis. There have been many reports of microbiome dysbiosis associated with several skin conditions, both pathological and non-pathological, for example aging. In aging skin, there are structural and functional changes in the skin that cause alterations in the microbiome habitat, resulting changes in the composition of the microbiota. This condition can cause dysbiosis, and it may also be a predisposing factor for the skin aging process. Objectives: To assess the correlation between the skin microbiome and the facial aging score of Indonesian women and also to determine the description of Indonesian young adults and elderly's women microbiomes, as well as addressing the differences in Shannon index and the relative abundance of skin microbiomes between young adult and elderly women. Methods: This research is an analytical observational study with cross-sectional design. Samples were taken in February – March 2023 at Cipto Mangunkusumo Hospital (RSCM), Dermatology and Venereology (DV) clinic. The research subjects were 48 people consisting of 24 healthy young adult women (21–37 years) and 24 elderly people (60–76 years) who passed the inclusion and exclusion criteria. The selected research subjects visited DV RSCM clinic, and underwent anamnesis, clinical examination, and facial examination using the JANUS™ III skin analyzer, also took skin swabs on both cheeks. The resulting skin swab samples were subjected to DNA extraction using DNeasy PowerSoil Kit™ and sequenced at V3-V4 16s rRNA region using the NGS (Next Generation Sequencing) tool, MiSeq Illumina™. Total were 39 DNA samples were obtained which could be identified by MiSeq Illumina™. Results: Abundance of the phylum Firmicutes and the genera Staphylococcus was significantly higher in elderly group. Shannon index of the young adult group was higher than the elderly group but was not stastistically ignificant and only weakly correlated with age (P>0.05). There is positive correlation between Staphylococcus and age, as well as Paracoccus and porphyrins. There is negative correlation between Shannon index and pores, Cutibacterium with porphyrins (P≤0.05) Conclusion: The final research results were obtained from 39 skin swab samples that were successfully identified by NGS tool. It is necessary to carry out future research that compares facial skin microbiome sampling methods to standardize further skin mikrobiome research and also to carry out research with a larger number of samples and multi-centered to represent rural areas of Indonesia with different climates and environments from urban communities (Jakarta)."

"Rare-actinomycetes tersebar di berbagai habitat terutama di habitat ekstrem seperti kawasan geotermal. Penelitian mengenai rare-actinomycetes dilakukan terkait potensinya sebagai penghasil senyawa bioaktif baru yang bermanfaat dalam bidang kesehatan, industri dan farmasi. Tujuan dari penelitian ini adalah mengisolasi dan mengkarakterisasi secara fenotip dan genotip rare-actinomycetes dari sampel tanah di bawah batuan kuarsa (S3.5.3) di hutan kawasan geotermal Cisolok. Metode pengayaan sampel tanah dilakukan menggunakan medium 10% R2A (Reasoner’s 2A) cair dengan penambahan cycloheximide 100 ppm dan sodium azide 60 ppm, kemudian diinkubasi pada suhu 30°C selama 30 hari. Isolasi rare actinomycetes dilakukan dengan metode membran filter dan spread pada medium 10% R2A gellan gum yang diinkubasi pada suhu 45°C. Karakterisasi dilakukan secara genotipik (berdasarkan data sequence gen 16S rRNA, analisis homologi sequence, dan rekonstruksi pohon filogenetik dengan metode Neighbour-Joining, Maximum Likelihood, dan Minimum Evolution); dan karakterisasi fenotipik (morfologi, fisiologi, dan biokimia). Sebanyak 26 isolat diperoleh dari sampel S3.5.3. Lima isolat dengan karakter morfologi actinomycetes yang diisolasi dari suhu 45°C dengan membran filter, dipilih untuk diidentifikasi. Hasil analisis sequence gen 16S rRNA dari lima isolat menunjukkan persentase homologi sebesar 95,46-99,56% terhadap Micromonospora yasonensis DS3186T. Berdasarkan hasil analisis filogenetik dengan metode Neighbour-Joining, kelima isolat memiliki hubungan kekerabatan terdekat dengan Micromonospora yasonensis DS3186T. Kelima isolat merupakan anggota class Actinomycetes, ordo Micromonosporales, family Micromonosporaceae. Karakter fenotipik kelima isolat sesuai dengan Micromonospora yasonensis sebagai spesies terdekatnya. Kelima isolat merupakan bakteri termotoleran (tumbuh pada suhu 30-45°C dan suhu optimum 40°C), aerobik, Gram positif, menghasilkan miselium substrat tanpa adanya miselium aerial, positif katalase, dan menghasilkan soluble pigment. Penelitian ini mengungkapkan bahwa Micromonospora yasonensis dapat ditemukan di kawasan geotermal dan berasosiasi dengan batuan kuarsa.

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Rare-actinomycetes are distributed in various habitats, particularly in extreme environments such as geothermal areas. Research on rare-actinomycetes focuses on their potential as producers of new bioactive compounds beneficial in health, industrial, and pharmaceutical fields. The aims of this study were to isolate and characterize rare-actinomycetes from soil samples beneath quartz rocks (S3.5.3) in the geothermal forest of Cisolok. Soil sample enrichment was performed using 10% R2A (Reasoner’s 2A) liquid medium supplemented with 100 ppm cycloheximide and 60 ppm sodium azide, incubated at 30°C for 30 days. Rare actinomycetes isolation was carried out using the membrane filter method and spread on 10% R2A agar with gellan gum, incubated at 45°C. Characterization included genotypic analysis based on the sequence of 16S rRNA gene, supported by phenotypic characterization (morphology, physiology, and biochemistry). A total of 26 isolates were obtained from sample S3.5.3. Five isolates with actinomycetes morphology isolated at 45°C using the membrane filter method were selected for characterization. Analysis of the 16S rRNA gene sequences from these five isolates showed homology levels of 95.46-99.56% to Micromonospora yasonensis DS3186T. Based on phylogenetic analysis using the Neighbour-Joining method, the five isolates were most closely related to Micromonospora yasonensis DS3186T. These isolates belong to the class Actinomycetes, order Micromonosporales, and family Micromonosporaceae. Phenotypic characteristics of the five isolates were consistent with Micromonospora yasonensis as their closest species. These isolates are thermotolerant bacteria (growing at temperatures of 30-45°C; optimum temperature 40°C), aerobic, Gram-positive, produce substrate mycelium without aerial mycelium, positive for catalase, and produce soluble pigment. This study reveals that Micromonospora yasonensis can be found in geothermal areas associated with quartz rocks."