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"Kortisol atau hormon stress, merupakan hormon steroid yang menjadi biomarker tubuh dan disekresikan oleh kelenjar adrenal saat tubuh merepson stres yang dihadapi. Ketidakseimbangan kadar kortisol dalam tubuh dapat menyebabkan berbagai gangguan fisiologis seperti stres kronis, penyakit Addison, Cushing, hingga depresi. Untuk itu, kadar kortisol dalam tubuh penting untuk diketahui dan dideteksi. Dalam penelitian ini, dikembangkan sensor elektrokimia berbasis screen-printed carbon electrode (SPCE) yang dimodifikasi dengan nanopartikel emas (AuNPs) dan molecularly imprinted polymers (MIPs) untuk deteksi kortisol secara selektif tanpa menggunakan antibodi. MIPs disintesis melalui elektropolimerisasi dengan p-ATP, TBAP dan kortisol sebagai molekul template. Deteksi kortisol dilakukan menggunakan voltametri siklik (CV) dan differential pulse voltammetry menunjukkan sensitivitas sebesar 0,0040 μA.mL/ng, LOD 2,07 ng/mL, dan LOQ 6,26 ng/mL pada rentang konsentrasi 10-50 ng/mL. Selain itu, sensor menunjukkan arus yang tidak berubah secara signifikan pada uji keberulangan dengan %RSD sebesar 3,87% (< 5%) dan uji keterulangan dengan %RSD sebesar 0,027%.

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Cortisol or stress hormone, is a steroid hormone that is a biomarker of the body and is secreted by the adrenal glands when the body responds to stress. An imbalance in cortisol levels in the body can cause various physiological disorders such as chronic stress, Addison's disease, Cushing's, and depression. For this reason, cortisol levels in the body are important to know and detect. In this study, a screen-printed carbon electrode (SPCE) based electrochemical sensor was developed modified with gold nanoparticles (AuNPs) and molecularly imprinted polymers (MIPs) for selective cortisol detection without the use of antibodies. MIPs are synthesized through electropolymerization with p-ATP, TBAP and cortisol as template molecules. Cortisol detection was carried out using cyclic voltametry (CV) anddifferential pulse voltammetry showed a sensitivity of 0,0434 μ A.mL/ng, LOD 52,48 ng/mL, and LOQ 159 ng/mL in the concentration range of 10-50 ng/mL. In addition, the sensor showed a significantly unchanged current on the repeatability test with a %RSD of 3,87% (< 5%) and the reproducibility test with a %RSD of 0,027%."

"Molecularly Imprinted Polymers (MIPs) is a type of macromolecule formed by application of molecularly imprinting technology, which creates cavities in synthetic polymeric matrices which are highly selective to an imprinted template. MIPs were synthesized experimentally using methacrylic acid (MAA), divinylbenzene-80 (DVB-80), azobisisobutyronitrile (AIBN) and methanol as a monomer, cross-linker, initiator and porogen, respectively. The Non-Imprinted Polymer (NIP) was produced simultaneously to serve as a control polymer. The rational design approach was theoretically conducted by Ab Initio Molecular Orbital Studies using Gaussian 09 computational software package at the theoretical level of DFT B3LYP/6-31 (d,p). The basis set is used to optimize the number of monomers and their binding site with the template. In both studies, MIPs were prepared with different ratios of template to monomer form a complex of 1:3, 1:4 and 1:5. Experimentally, the MIPs synthesized via precipitation polymerization technique produced homogenous spherical beads distribution where the complex 1:3 gave the best. Theoretical studies support this experimental finding where the complex 1:3 gave the highest interaction energy between caffeine and MAA, -45.29 kJ/mol followed by 1:4, -43.52 kJ/mol and 1:5, -43.11 kJ/mol."