Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
Latar belakang: Trietylene glycol dimethacrylate (TEGDMA) merupakan salah satu monomer yang terkandung dalam resin komposit. Jika polimerisasi resin komposit tidak sempurna, TEGDMA dapat terlepas ke dalam rongga mulut dalam beberapa menit hingga jam dan dapat berpenetrasi mencapai pulpa. TEGDMA dilaporkan bersifat toksik terhadap sel dan jaringan rongga mulut. Tujuan: Mengetahui efek TEGDMA terhadap sel-sel pulpa gigi ditentukan berdasarkan viabilitas dan profil protein sel pulpa (in vitro). Metode: Sel-sel pulpa berasal dari jaringan pulpa gigi sehat yang baru diekstraksi, kemudian dikultur dalam DMEM (37o C, 5% CO2) sampai confluent (± 2 malam). Selanjutnya dilakukan subkultur dengan kondisi yang sama selama 1 malam pada 24-wellplate. Kemudian pada kelompok perlakuan dipaparkan TEGDMA dengan konsentrasi 4 mM, 8 mM dan 12 mM selama 24 jam; sedangkan pada kelompok kontrol tidak dipaparkan TEGDMA. Viabilitas sel diukur dengan menggunakan MTT assay dan hasilnya dibaca dengan microplate reader (490 nm), sedangkan gambaran profil protein dideteksi dengan menggunakan SDS-PAGE dan diinterpretasikan dengan menggunakan Gel Doc. Hasil: Rerata optical density (OD) ± SD kelompok perlakuan TEGDMA 4 mM (1,71 ± 0,08); 8 mM (1,59 ± 0,11); dan 12 mM (1,50 ± 0,16) lebih rendah dibandingkan dengan kelompok kontrol (1,81 ± 0,11). Uji statistik One Way ANOVA menunjukkan bahwa nilai rerata OD kelompok TEGDMA 8 mM dan 12 mM berbeda bermakna dengan kelompok kontrol (p<0.05). Profil protein sel mengalami perubahan setelah pemaparan TEGDMA. Kesimpulan: Pada penelitian ini viabilitas sel menurun dan terjadi perubahan profil protein sel setelah pemaparan TEGDMA.

---

Background: Trietylene glycol dimethacrylate (TEGDMA) is one of monomer contained in composite resin. If the polimerized was incomplete TEGDMA could bereleased into oral cavity in minutes to hours and could penetrate to the dental pulp. Itwas reported that TEGDMA has cytotoxic effects to cells and tissues in oral cavity. Objectives: To determine the toxic effect of TEGDMA on dental pulp cells culture based on cell viability and Protein Cell Profile. Methods: The pulp cells were isolated from the pulp tissue of the freshly extracted teeth, cultured in DMEM (37o C, 5% CO2) until confluent (± 2 nights). Afterwards, subcultured with the same condition overnight in 24-wellplate. Then, the treatment groups were treated with TEGDMA 4 mM, 8 mM, dan 12 mM for 24 hours, whereas in control group without TEGDMA exposure. The optical density of cell viability was measured by MTT assay then it was read with microplate reader in 490 nm. The protein cell profile was identified by SDS-PAGE method and analyzed by Gel Doc. Results: Mean optical density ± SD of TEGDMA treatment group 4mM (1,71 ± 0,08), 8mM (1,59 ± 0,11), and 12 mM (1,50 ± 0,16) were lower than the control group (1,81 ± 0,11). One Way ANOVA analysis showed that TEGDMA treatment group 8 mM and 12 mM had significant differences compared with the control group (p<0,05). The protein profile of cells was altered after TEGDMA exposure. Conclusion: In this research the cell viability was decreased and the protein profile of cells was altered after TEGDMA exposure.

Abstrak :
Latar belakang: Trietylene glycol dimethacrylate (TEGDMA) merupakan salah satu monomer yang terkandung dalam bahan tambal resin komposit. Jika polimerisasi tidak sempurna, TEGDMA dapat dengan mudah terlepas ke dalam rongga mulut beberapa menit hingga jam setelah penambalan, dan dapat berpenetrasi ke dalam pulpa. TEGDMA yang terlepas dilaporkan bersifat toksik terhadap jaringan dan sel tubuh. Tujuan: menentukan efek TEGDMA terhadap protein total dan profil protein sel-sel pulpa gigi. Metode: sel-sel pulpa didapat dari jaringan pulpa gigi sehat yang baru diekstraksi, kemudian dikultur pada medium kultur DMEM. Setelah sel kultur tampak confluent (±2 malam), dilakukan subkultur dari kultur primer tersebut, yang kemudian diinkubasi kembali pada suhu 37° C dan 5% CO2 selama 1 malam pada 24- well plate. Kemudian dilakukan pemaparan TEGDMA dengan konsentrasi 4mM, 8mM dan 12mM, selama 24 jam, sedangkan pada kelompok kontrol tidak dilakukan pemaparan TEGDMA. Penentuan konsentrasi protein total sel pulpa dilakukan dengan menggunakan Bradford protein assay. Profil protein sel pulpa diidentifikasi dengan teknik SDS-PAGE. Analisa berat molekul protein sampel dilakukan dengan menggunakan Gel Doc (Band Analysis Quick Guide). Hasil: Rerata konsentrasi protein total sel (µg/ml ± SD) pada kelompok perlakuan dengan TEGDMA 4mM (22762,27±3385,87) dan 8mM (20268,44±1701,14) memiliki nilai dibawah kelompok kontrol (24253,77±3072,88). Sedangkan kelompok perlakuan dengan TEGDMA 12mM (23706,51±3214,52) memiliki nilai konsentrasi protein total sel di atas kelompok 4mM dan 8mM, namun masih tetap di bawah kelompok kontrol. Berdasarkan uji statistik dengan one way ANOVA, hanya kelompok TEGDMA 8mM yang memiliki perbedaan rerata konsentrasi protein total sel yang bermakna (p=0.037) terhadap kelompok kontrol. Selanjutnya dari gambaran profil protein yang terbentuk pada gel elektroforesis, tampak perubahan profil protein sel pada setiap kelompok setelah paparan TEGDMA. Kesimpulan: Pada penelitian ini terjadi penurunan konsentrasi protein total sel dan perubahan profil protein sel pulpa gigi setelah pemaparan TEGDMA dengan konsentrasi 4mM, 8mM, dan 12mM.

---

Background: Trietylene glycol dimethacrylate (TEGDMA) is one of monomer that contained in composite resin restoration. When polymerization is incomplete, this compound could leache into oral cavity in minutes to hours after the restoration, and could penetrate the dental pulp. TEGDMA has been reported to be cytotoxic to the tissues and cells. Objective: to determine the effects of TEGDMA on total protein and protein profile of the dental pulp cells. Methods: the dental pulp cells were collected from the dental pulp tissues of the freshly extracted teeth, and cultured in culture medium of DMEM. After the growth of cultured cells was confluent (±2 nights), the cells were subcultured then incubated (37°C, 5% CO2) overnight in 24-well plate. Afterwards, the cells were exposed to TEGDMA with concentrations of 4mM, 8mM, and 12mM, for 24 hours, meanwhile in control group without TEGDMA exposure. The concentration of total cell protein was measured by Bradford protein assay. The protein profile of the dental pulp cells were identified by SDS-PAGE. The molecular weight of sample protein was analyzed by Gel Doc (Band Analysis Quick Guide). Results: The mean of total cell protein concentration (µg/ml ± SD) on treatment groups of 4mM TEGDMA (22762,27±3385,87) and 8mM (20268,44±1701,14) were lower than the controls (24253,77±3072,88). Whereas, the total cell protein concentration of treatment group of TEGDMA 12mM (23706,51±3214,52) was higher than the treatment groups with TEGDMA 4mM and 8mM, but it was still lower than the controls. According to one way ANOVA statistic test, only the treatment group with TEGDMA 8mM was significantly lower than the controls (p=0.037). Furthermore, the protein profile identified by electrophoresis gel, showed the profile alteration after TEGDMA exposure. Conclusion: In this research the total cell protein concentration was decreased and the protein profile of the dental pulp cells was altered after exposure with TEGDMA 4mM, 8mM, and 12mM.

Abstrak :
Latar belakang: Trietylene glycol dimethacrylate (TEGDMA) merupakan salah satu monomer yang terkandung dalam bahan tambal resin komposit. Jika resin komposit mengalami polimerisasi tidak sempurna, TEGDMA dengan mudah terlepas ke dalam rongga mulut dalam beberapa menit hingga jam setelah penambalan, kemudian berpenetrasi mencapai pulpa. TEGDMA yang terlepas dilaporkan bersifat sitotoksik. Tujuan: Menentukan efek TEGDMA (4 mM, 8 mM, dan 12 mM) terhadap protein total dan profil protein medium kultur sel-sel pulpa gigi. Metode: Sel-sel pulpa didapat dari jaringan pulpa gigi sehat yang baru diekstraksi, kemudian dikultur pada medium kultur DMEM. Setelah sel kultur tampak confluent (±2 malam), dilakukan subkultur yang kemudian digunakan sebagai sampel pada penelitian ini. Selanjutnya, kultur sel-sel pulpa gigi pada kelompok perlakuan masing-masing dipapar TEGDMA 4 mM, 8 mM, dan 12 mM. dan diinkubasi pada suhu 37°C, 5% CO2 selama 24 jam. Sedangkan pada kelompok kontrol tidak dipaparkan TEGDMA. Konsentrasi protein total medium kultur diukur menggunakan Bradford protein assay lalu dibaca dengan microplate reader pada panjang gelombang 655 nm. Kemudian, identifikasi profil protein medium kultur dilakukan dengan metode SDS PAGE. Hasil: Nilai rerata konsentrasi protein total medium kultur (µg/ml ± SD) kelompok perlakuan TEGDMA 4 mM, 8 mM, dan 12 mM berturut turut adalah 28635.85 ± 2373.39, 35288.41 ± 3469.47, dan 38199.79 ± 2752.47. Nilai-nilai tersebut lebih tinggi daripada kelompok kontrol 27073.83 µg/ml ± 2772.46. Analisis statistik one way ANOVA menunjukan terdapat perbedaan bermakna antara kelompok perlakuan TEGDMA 8 mM dan 12 mM dengan kelompok kontrol (p<0,05). Hasil identifikasi profil protein medium kultur menunjukan tampak perubahan profil protein pada setiap kelompok setelah pemaparan TEGDMA 4 mM, 8 mM, dan 12 mM. Kesimpulan: Pada penelitian ini konsentrasi protein total medium kultur meningkat dan profil protein medium kultur mengalami perubahan setelah pemaparan TEGDMA 4 mM, 8 mM, dan 12 mM pada sel-sel pulpa.

---

Background: Trietylene glycol dimethacrylate (TEGDMA) is one of monomer that contained in composite resin restoration. TEGDMA could be released from composite resins to the oral cavity following incomplete polymerization in a few minutes to hours after the placement of restoration, and then the monomers of TEGDMA could penetrate the dental pulp. TEGDMA that released was reported has cytotoxic effects. Objectives: To determine the effect of TEGDMA (4 mM, 8 mM, dan 12 mM) on total protein and protein profile of culture medium of the dental pulp cells. Methods: The pulp cells were isolated from the pulp of the freshly extracted teeth, and cultured in culture medium of DMEM. After the cells visually confluent (±2 nights), subcultured to be used as samples. Afterwards, the cells culture in treatment group were treated with TEGDMA 4 mM, 8 mM, dan 12 mM and incubated at 37°C, 5% CO2 for 24 hours. Whereas, in control group without TEGDMA exposure. The concentration of total protein in culture medium was measured by Bradford protein assay then were read by microplate reader in 655 nm. Then, the protein profile of culture medium was identified by SDS PAGE method. Result: The mean of total protein of culture medium (µg/ml ± SD) on treatment groups of TEGDMA 4 mM, 8 mM, dan 12 mM were 28635.85 ± 2373.39, 35288.41 ± 3469.47, and 38199.79 ± 2752.47 were higher than the controls 27073.83 ± 2772.46. One way ANOVA statistic analysis showed that treatment group of TEGDMA 8 mM and 12 mM were significant different compared with the control group (p<0,05). The protein profile of culture medium was altered after TEGDMA exposure. Conclusion: In this research the total protein of culture medium was increased and its protein profile was altered after exposure of TEGDMA 4 mM, 8 mM, and 12 mM to dental pulp cells.