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"ABSTRAKAchyranthes aspera atau dalam Bahasa Indonesia biasa disebut Sangketan merupakan tumbuhan liar yang sering digunakan sebagai obat tradisional. Akar Achyranthes aspera ini dapat berkhasiat sebagai penyembuh luka dengan melibatkan peran arginase, arginin, dan metabolitnya yaitu nitrit oksida yang memengaruhi secara langsung proses penyembuhan luka tersebut. Penelitian ini bertujuan untuk mengetahui potensi dari ekstrak akar Achyranthes aspera dalam menghambat aktivitas arginase. Simplisia diekstraksi secara bertingkat dengan pelarut n-heksana, etil asetat, dan metanol dengan metode ultrasound-assisted extraction. Ekstrak yang dihasilkan dari masing-masing pelarut kemudian diuji penghambatannya terhadap aktivitas arginase menggunakan metode kolorimetri dengan microplate, lalu dilakukan penetapan kadar fenol total dan kadar flavonoid total. Uji penghambatan aktivitas arginase oleh ekstrak n-heksana, etil asetat, dan metanol pada konsentrasi 100 g/ml secara berurutan adalah 9,56; 17,58; dan 29,77; kandungan fenol total secara berurutan adalah 3,91; 4,83; dan 11,18 mgGAE/gram sampel serta kandungan flavonoid total secara berurutan adalah 0,29; 0,80; dan 0,88 mgQE/gram sampel. Dari penelitian ini dapat disimpulkan bahwa ekstrak akar Achyranthes aspera memiliki potensi penghambatan aktivitas arginase yang rendah.

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ABSTRACTAchyranthes aspera, or commonly called as Sangketan in Indonesian is a wild plant that is used as a traditional medicine. The roots of Achyranthes aspera can be used as a wound healer by involving the role of arginine and its metabolites, nitric oxide, that directly affect the wound healing process itself. The aim of this study was to determine the potential of Achyranthes aspera roots extract in inhibiting arginase activity. The simplicia is extracted using ultrasound assisted extraction method with n hexane, ethyl acetate, and methanol solvent. Each extract from different solvents were tested for the inhibition of arginase activity using colorimetric method with microplate, determination of total phenolic concentration, and total flavonoid concentration. The results of inhibition test of arginase activity by n hexane, ethyl acetate, and methanol extract in sequence are 9.56, 17.58 and 29.77 at concentration of 100 g/ml the total phenol concentration in sequence are 3.91 4.83 dan 11.18 mgGAE gram of sample and the total flavonoid concentration in sequence are 0.29 0.80 and 0.88 mgQE gram of sample. From this research it can be concluded that Achyranthes aspera roots extract had low potency of arginase inhibitory activity."

"Tanaman sangketan Achyranthes aspera L. merupakan tanaman yang memiliki banyak khasiat. Dalam upaya pengembangan obat tradisional, proses penjaminan mutu dan keamanan obat perlu dilakukan yakni dengan standardisasi. Standardisasi dilakukan terhadap simplisia dan ekstrak etanol akar dan daun sangketan yang berasal dari tiga daerah yakni Sragen, Klaten, dan Boyolali. Proses ekstraksi menggunakan metode maserasi dengan pelarut etanol 70. Hasil pengujian terhadap parameter spesifik simplisia akar sangketan yakni kadar sari larut etanol 3,07-4,28 ; kadar sari larut air 6,16-7,48 ; pola kromatogram menggunakan standar -sitosterol, serta kadar fenol total 1,58-1,82 mgGAE/gram simplisia. Hasil pengujian parameter non spesifik simplisia akar sangketan yakni susut pengeringan 5,25-7,28 ; kadar abu total 12,12-17,62 dan kadar abu tidak larut asam 0,87-1,32. Parameter spesifik ekstrak akar sangketan yakni kadar fenol total 33,90-36,88 mgGAE/gram ekstrak. Parameter non spesifik ekstrak akar sangketan antara lain kadar abu total 6,26-9,28 ; kadar abu tidak larut asam 0,10-0,12 ; dan kadar air 5,29-6,75. Sementara itu hasil pengujian terhadap parameter spesifik simplisia daun sangketan yakni kadar sari larut etanol 5,83-9,36 ; kadar sari larut air 10,25-15,44 ; pola kromatogram menggunakan standar -sitosterol. Kadar fenol total 0,93-1,15 mgGAE/g simplisia. Parameter non spesifik simplisia daun sangketan antara lain susut pengeringan 15,25-15,91 ; kadar abu total 14,58-20,79 dan kadar abu tidak larut asam 1,75-2,19. Parameter spesifik ekstrak daun sangketan yakni kadar fenol total 6,94-7,68 mgGAE/gram ekstrak. Parameter non spesifik ekstrak daun sangketan kadar abu total 13,18-14,52 ; kadar abu tidak larut asam 0,14-0,29 ; dan kadar air 10,42-11,16. ......Sangketan Achyranthes aspera L. is one of many plants which have many efficacies. In the recent development of the traditional medicine, there is a need to have quality assurance and drug security process by standardization. Standardization was carried out on simplicial and ethanolic extract of roots and leaves of sangketan which come from three different regions Sragen, Klaten and Boyolali. Extraction method was maceration with ethanolic 70 as its solvent. The result of specific parameters of roots simplicia of sangketan plant showed ethanol soluble extract 3.07 4.28 water soluble extract 6.16 7.48, chromatogram pattern used sitosterol as standard, and total phenolic content 1.58 1.82 mgGAE g simplicia. The results of non specific parameters of roots simplicia of sangketan plant showed loos on drying 5.25 7.28 total ash value 12.12 17.62 acid insoluble ash 0.87 1.32. The result of specific parameters of roots rsquo extract of Sangketan plant showed total phenolic content of 33.90 36.88 mgGAE g extract. The result of non specific parameters of roots extract showed total ash value of 6.26 9.28 acid insoluble ash value of 0.10 0.12 and moisture content of 5.29 6.75. Meanwhile, the result of specific parameters of leaves rsquo simplicia of sangketan plant showed ethanol soluble extract 5.83 9.36 water soluble extract 10.25 15.44 chromatogram pattern used sitosterol as standard, and total phenolic content 0.93 1.1,5 mgGAE g simplicia. The result of non specific parameters of leaves rsquo simplicia of sangketan plant showed loss on drying of 15.25 15.91 total ash value of 14.58 20.79 and acid insoluble ash value of 1.75 2.19. The result of specific parameters of leaves extract of sangketan plant showed total phenolic content of 6.94 7.68 mgGAE g extract. The result of non specific parameters of leaves rsquo extract of sangketan plant showed total ash value of 13.18 14.52 acid insoluble ash value of 0.14 0.29 and moisture content of 10.42 11.16."