Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
Tujuan penelitian ini adalah untuk mengetahui efek in vitro kalium iodida (KI) pada produksi fosfatase asam dengan mengkarakterisasikan secara lengkap strain S.schenckii yang diisolasi dari seorang pasien dengan sporotrichosis kulit. Enzim fosfatase asam diukur selama 3 fase pertumbuhan S. schenckii, dengan dan tanpa KI yang ditambahkan pada medium kultur dengan 3 konsentrasi berbeda. Pada kontrol dan bahan uji dengan konsentrasi KI yang berbeda, tidak dijumpai efek samping KI pada produksi fosfatase asam, pada fase awal dan “mid-log” pertumbuhan jamur. Sedangkan pada fase eksponensial dari bahan uji tampak penurunan produksi enzim yang bermakna secara statistik dengan kadar KI 0,8% dan 3,2%. Aktivitas yang rendah pada kadar KI 0,8% dan 3,2% menunjukkan bahwa KI mempunyai efek hambatan pertumbuhan S.schenkii dan menyebabkan penurunan aktivitas enzim. (Med J Indones 2003; 12: 65-8)

---

The present study was undertaken to find out the in-vitro effect of potassium iodide (KI) on the production of acid phosphatase by fully characterized strain of S.schenckii isolated from a patient of Cutaneous Sporotrichosis. The enzyme acid phosphatase was estimated during the 3 phases of growth of S.schenckii, without and with three concentrations of KI incorporated in the culture medium. In the control and in the test proper, with various concentrations of KI, no adverse effect of KI was observed on the production of acid phosphatase in early and mid log phase of fungal growth. Whereas in the exponential phase in test proper, there was a statistical significant decrease in the enzyme production with 0.8% and 3.2% of KI. The low activity at 0.8% and 3.2% KI indicates that KI has inhibitory effect on the growth of S.schenckii and has led to decrease in the activity of the enzyme. (Med J Indones 2003; 12: 65-8)

Abstrak :
Fosfatase asam merupakan enzim yang dapat digunakan untuk teknik ELISA - untuk mengukur kadar suatu zat dalam cairan tubuh yang jumlahnya sangat kecil. Salah satu sumber enzim tersebut adalah air kelapa (Cocos nucifera Linn). Penelitian yang dilakukan oleh Sadavisan (1951), Wilson et al. (1952), Anna Istiqomah (1994) dan Emilia Aisjah (1996) menunjukkan adanya aktivitas enzim fosfatase dalam daging buah dan air kelapa. Penelitian ini adalah mengisolasi dan pemurnian enzim fosfatase asam dari air kelapa. Untuk pemurnian enzim digunakan kromatografi gel dengan Sephadex G-75 dan kromatografi afinitas dengan Gel immobilized p-aminobenzyl phosphonic acid sepharose. Kemurnian enzim diperiksa dengan elektroforesis gel poliakrilamid. Aktivitas enzim secara kuantitatif ditentukan dengan spektrofotometer, dan secara kualitatif dapat dilihat dengan pewarnaan substrat. Kadar protein diukur dengan spektrofotometer pada panjang gelombang 280 nm. Hasil.penelitian ini menunjukkan bahwa dalam air kelapa terdapat enzim fosfatase asam yang ditunjukan oleh satu pita pada elektroforesis gel poliakrilamid.

---

Isolation And Purification Of Acid Phosphatase From Coconut Water (Cocos nucifera Linn)Acid phosphatase was detected in coconut. More than fourty years ago, early investigators found this enzyme solely in coconut milk. It is not until recently that the enzyme was also found in coconut water. Recent studies also reported some characteristics of the enzyme from both sources. The purpose of this study is to isolate and to purity acid phosphatase from coconut water. Firstly, the enzyme was precipitated with etanol. After the dialysis in a cellophane bag, the precipitate was redissolved in 0,9% NaCl. The enzyme was separated with Sephadex 0-75, using 0,9% NaCl as eluent. Fractions of 5 mL were collected and each was analysed for the protein contents and for acid phosphatase activities. The fractions of high enzymes activity were pooled and purified, using an affinity chromatography technique with benzoyl phosphonic acid, a competitive inhibition, as a stationary immobilized ligand. Retained enzymes were eluted with 0,2 M NaHHPO4 and fractions of 2 mL were collected. During the study, protein contents were measured with A 280 technique and enzymes activities were assayed with p-nitrophenyl phosphate (p-NPP) as a substrate. In both steps, the purity of the pooled fractions were analysed with a polyacrylamide gel electrophoresis, stained with Coomassie Blue as well as p-NPP-ammonium sulfide, lead acetat for revealing the enzyme.It is found that acid phosphatase could be isolated and purified, as indicated by increasing specific activity and by PAGE analysis, from coconut water, by gel filtration and affinity chromatography technique.

Abstrak :
Pendekatan sistem imun pada pejamu M. tuberculosismerupakan salah satu pilihan dalam pengembangan terapi tuberkulosis, terutama pada kasus tuberkulosis (TB)  resisten obat. Tujuan penelitian ini adalah untuk menganalisis perbedaan fungsi makrofag pada penderita TB resisten obat dibandingkan dengan kontak erat yang terinfeksi laten dan sehat. Sel Monosit Darah Tepi (SMDT) diisolasi dan dikultur selama 7 hari. Fagositosis dinyatakan jika terdapat minimal satu sel darah merah domba tampak melekat pada membran makrofag. Kemampuan lisosom diperiksa dengan uji aktivitas enzim fosfatase asam. Enam pasien TB-RO dan 18 kasus kontak erat (8 TB laten;10 sehat) di RS Universitas Indonesia direkrut sebagai subjek penelitian. Hasil menunjukkan bahwa aktivitas fagositosis kelompok infeksi laten lebih tinggi dibandingkan kelompok sehat dan TB RO (one-way ANOVA, p<0,05). Aktivitas enzim fosfatase asam lebih tinggi pada kelompok TB RO. Perbedaan fungsi makrofag ini diharapkan dapat menjadi referensi selanjutnya dalam terapi TB RO ataupun terapi pencegahan.  ......The immune system approach to the host of M. tuberculosis is an option in developing tuberculosis therapy, especially in drug-resistant tuberculosis (DR-TB) cases. This study aimed to analyze the differences in macrophage function in drug-resistant TB patients compared to close contacts who were latently infected and healthy. Peripheral Blood Mononuclear Cell (PMBC) was isolated and cultured for seven days. Phagocytosis is expressed when at least one sheep red blood cell appears attached to the macrophage membrane. The ability of lysosomes was examined by testing the activity of the acid phosphatase enzymes. Six DR-TB patients and 18 close contact cases (8 LTBI; 10 healthy) at Universitas Indonesia Hospital were recruited as research subjects. The results showed that the phagocytosis activity of the latent infection group was higher than that of the healthy and TB RO groups (one-way ANOVA, p<0.05). Acid phosphatase activity was higher in the DR-TB group. The difference in macrophage function is expected to be a further reference in DR-TB treatment or preventive therapy.

Abstrak :
Latar belakang. Spermatozoa harus mempunyai motilitas yang baik agar dapat tercapainya fertilisasi alami. Gangguan pada kelenjar assesori pria merupakan salah satu penyebab astenozoospermia, namun pemeriksaan pada kelenjar assesori pria jarang dilakukan. Kadar asam sitrat dalam plasma seminalis paling besar bila dibandingkan hasil sekresi kelenjar assesori lainnya, hal ini mendasari peneliti untuk melakukan penelitian terhadap asam sitrat dan produk utama yang dihasilkan kelenjar prostat lainnya yaitu fosfatase asam. Asam sitrat diduga berperan dalam proses viskositas, pH semen sehingga dapat mempengaruhi motilitas sperma, fosfatase asam diduga mempengaruhi pula motilitas sperma serta turut berperan dalam menjaga keseimbangan pH semen. Metodologi. Penelitian ini menggunakan desain potong lintang pada 57 sampel seminal plasma. Didapatkan 30 sampel seminal plasma kelompok motilitas normal dan 27 sampel seminal plasma kelompok astenozoospermia. Pemeriksaan pada analisa semen standar didapatkan viskositas, pH , volume, motilitas dan konsentrasi spermatozoa. Pemeriksaan kadar asam sitrat pada plasma seminalis dengan metode Flint, dan pemeriksaan aktivitas fosfatase asam pada seminal plasma menggunakan metode spektrofotometri. Hasil. Hasil perbandingan volume semen, konsentrasi sperma dan kadar asam sitrat pada sampel plasma seminalis dengan astenozoospermia lebih rendah dibandingkan sampel plasma seminalis dengan motilitas normal, sebaliknya hasil pebandingan viskositas dan aktivitas fosfatase asam pada sampel plasma seminalis dengan astenozoospermia lebih tinggi dibandingkan sampel plasma seminalis dengan motilitas normal. Hasil pemeriksaan pH pada kedua kelompok sampel menunjukkan kecenderungan karakteristik yang sama pada kedua kategori. Hasil perbandingan nilai rerata kadar asam sitrat pada semua kategori konsentrasi sperma menunjukkan kadar lebih rendah pada sampel plasma seminalis dengan astenozoospermia dibandingkan dengan kelompok motilitas normal, sebaliknya pada hasil perbandingan nilai rerata aktivitas fosfatase asam pada semua kategori konsentrasi sperma menunjukkan kadar lebih tinggi pada sampel plasma seminalis dengan astenozoospermia dibandingkan dengan kelompok motilitas normal. Kesimpulan. Kadar asam sitrat, volume dan konsentrasi dalam plasma seminalis pada sampel astenozoospermia lebih rendah dibandingkan sampel dengan motilitas normal, perbedaan ini signifikan secara statistik. Begitupun pada semua tingkat konsentrasi sperma nilai rerata kadar asam sitrat pada sampel astenozoospermia memiliki kecenderungan lebih rendah dibandingkan sampel dengan motilitas normal, perbedaan ini tidak signifikan secara spesifik. Aktivitas fosfatase asam dan viskositas pada sampel plasma seminalis dengan astenozoospermia lebih tinggi dibandingkan sampel dengan motilitas normal, perbedaan ini signifikan secara statistik, pada semua tingkat konsentrasi sperma aktivitas fosfatase asam pada plasma seminalis dengan astenozoospermia memiliki kecenderungan lebih tinggi dibandingkan dengan motilitas normal namun perbedaan ini tidak signifikan secara statistik. Penelitian ini menunjukkan kadar asam sitrat dan aktivitas fosfatase asam tidak mempengaruhi spermatogenesis.

---

Background. Spermatozoa should have good motility in order to achieve a natural fertilization. Assesori male gland disorders are one of the causes astenozoospermia, but examination of the gland assesori rare Citric acid levels in the seminal plasma of the most substantial when compared to the results of other assesori gland secretions, it is the underlying researchers to conduct research on citric acid and primary products other prostate gland that is acid phosphatase. Citric acid is thought to play a role in the process viscosity, pH cement that can affect sperm motility, acid phosphatase is also thought to affect sperm motility as well as play a role in maintaining the pH balance of the semen. Methodology. This study used a cross-sectional design of the 57 samples of seminal plasma. Obtained 30 samples of seminal plasma of normal motility group and 27 samples of seminal plasma astenozoospermia group. Examination of the standard semen analysis obtained viscosity, pH, volume, motility and concentration of spermatozoa. Examination of citric acid levels in seminal plasma by the method of Flint, and examination of acid phosphatase activity in seminal plasma using spectrophotometric method. Result. The results of the comparison semen volume, sperm concentration and citric acid levels in seminal plasma samples with astenozoospermia lower than the seminal plasma samples with normal motility, otherwise Comparing the results of viscosity and acid phosphatase activity in seminal plasma samples with astenozoospermia higher than the seminal plasma samples with normal motility. PH probe results in both sample groups showed a trend similar characteristics in both categories. The results of comparison of the average levels of citric acid in all categories sperm concentration showed lower levels in seminal plasma samples with astenozoospermia compared with normal motility, whereas the mean value of the comparison results of acid phosphatase activity in all categories sperm concentration showed higher levels in seminal plasma samples with astenozoospermia compared with normal motility. Conclusion. Citric acid content, volume and concentration in seminal plasma on astenozoospermia sample was lower than samples with normal motility, this difference was statistically significant. Likewise at all levels of sperm concentration of citric acid levels mean value in astenozoospermia samples have a lower propensity than samples with normal motility, this difference was not significant specifics. Acid phosphatase activity and viscosity in the seminal plasma samples with astenozoospermia higher than samples with normal motility, these differences are statistically significant, at all levels of sperm concentration of acid phosphatase activity in seminal plasma with astenozoospermia have a higher tendency than normal motility, but this difference was not statistically significant. This study showed levels of citric acid and acid phosphatase activity does not affect spermatogenesis.