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Abstrak :
[ABSTRAK Kanker payudara merupakan salah satu penyakit mematikan di dunia. Pengembangan ekstrak kunyit sebagai tanaman yang memiliki aktivitas antiproliferasi dan toksisitas rendah dilakukan guna meminimalisasi efek samping terapi kanker. Ekstrak kunyit dienkapsulasi dengan liposom, sebuah vesikel lipid bilayer yang berfungsi sebagai pembawa obat kanker dalam tubuh. Penelitian ini bertujuan untuk mengetahui pengaruh enkapsulasi ekstrak etanol kunyit terhadap aktivitas antiproliferasi sel kanker payudara T47D secara in vitro. Liposom dibuat dengan metode lapis tipis dan dikecilkan ukuran partikelnya dengan ekstrusi. Bahan yang digunakan adalah fosfatidilkolin, kolesterol, dan ekstrak kunyit. Optimasi liposom dibuat dalam tiga formulasi dengan perbedaan jumlah ekstrak. Formulasi paling optimal adalah formulasi dengan jumlah ekstrak paling sedikit, dilihat dari parameter fisik, yaitu endapan paling halus dan waktu pengendapan paling lama. Liposom dievaluasi ukuran partikel dan zeta potensialnya dengan DLS, morfologinya dengan TEM, dan efisiensi penjerapannya dengan dialisis. Formulasi paling optimal diuji aktivitas antiproliferasinya dan dibandingkan dengan ekstrak yang tidak dienkapsulasi liposom dengan metode MTT. Hasilnya terdapat pengaruh penurunan aktivitas antiproliferasi ekstrak yang dienkapsulasi liposom. IC50 liposom ekstrak adalah 45,762 ppm dan IC50 ekstrak adalah 36,399 ppm. Ukuran partikel liposom adalah di bawah 445 nm. Zeta potensial liposom adalah -7,51 mV. Morfologi liposom adalah LUV dan MVV. Efisiensi penjerapan liposom adalah 63,80%.

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ABSTRACT , Breast cancer is one of deadliest diseases in world. Development turmeric extract as plants that have antiproliferative activity and low toxicity have done to minimize cancer therapy side effects. Turmeric extract encapsulated with liposome, a vesicle lipid bilayer that have function as cancer drug carrier in body. This research aimed to determine encapsulation effect of turmeric ethanol extract against antiproliferative activity in T47D breast cancer cells through in vitro assay. Liposomes was made using thin layer method and particle size reduced by extrusion. Materials was used phosphatidylcholine, cholesterol, and turmeric extract. Optimization liposomes was made in three formulations with different numbers of extracts. Most optimal formulation was formulation with minimum extracts, judging from physical parameters which have smallest precipitates and longest settling time. Evaluation liposome particle size and zeta potential was used DLS, morphology was used TEM, and entrapment efficiency was used dialysis. Most optimal formulation was tested their antiproliferative activity compared with not encapsulated extracts used MTT method. There was decreased antiproliferative activity of encapsulated extracts. IC50 encapsulated extracts was 45,762 ppm and IC50 extracts was 36,399 ppm. Liposome particle size was below 445 nm. Zeta potential was -7,51 mV. Morphology was LUV and MVV. Entrapment efficiency was 63,80%.]

Abstrak :
[ABSTRAK Tanaman sambiloto (Andrographis Paniculata Nees ) memiliki banyak manfaat dalam pengobatan, salah satunya sebagai obat antikanker. Liposom merupakan salah satu perkembangan dari sistem penghantaran obat yang telah diteliti dapat digunakan sebagai pembawa obat-obat, protein, dan zat?zat molekuler lain. Penelitian ini bertujuan untuk mengembangkan obat dengan bahan dasar ekstrak herba sambiloto yang dienkapsulasi liposom dan diuji aktivitas antiproliferasinya terhadap sel kanker payudara T47D. Metode yang digunakan dalam pembuatan liposom ini adalah metode hidrasi lapis tipis. Pengecilan ukuran partikel dilakukan dengan cara ekstrusi. Evaluasi yang dilakukan dalam penelitian ini adalah distribusi ukuran partikel dan zeta potensial dengan alat DLS, efisiensi penjerapan dengan alat dialisis, morfologi ukuran dengan alat TEM. Uji antiproliferasi sel kanker menggunakan metode MTT. Hasil yang diperoleh pada TEM yang menggambarkan model liposom OLV dengan ukuran ±50 nm; distribusi ukuran partikel liposom sebesar 452,5 dan 43,82 nm serta zeta potensial sebesar -11,3 mV; efisiensi penjerapan sebesar 61,63 %. IC50 dari liposom ekstrak dan larutan ekstrak berturut-turut adalah 11,997 ppm dan 27,488 ppm. Hasil ini menunjukkan bahwa enkapsulasi ekstrak herba sambiloto dengan liposom memberikan pengaruh terhadap aktivitas antiproliferasi sel kanker payudara T47D.

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ABSTRACT ;A Sambiloto?s Plant (Andrographis paniculata Nees) has many benefits in the medicals treatment, one of them as an anticancer drug. Liposomes are one of the development of drug delivery systems that have been studied can be used as carriers of drugs, proteins, and other molecular substances. This research aims to develop drugs with a basis of extract of bitter herbs are encapsulated with liposomes for comparison their antiproliferation activity against T47D breast cancer cells. The method which used in the manufacture of liposomes is thin layer hydration method. Reduction of particle size liposomes is done by extrusion. Evaluations were performed in this study is the particle size distribution and zeta potential by DLS, entrapment efficiency by dialisis, morphology size by TEM. Antiproliferation cancer cells test using MTT method. Results obtained at the TEM depicting the model OLV liposomes with a size of about ±50 nm; liposome particle size distribution of 441 and 45.3 nm, and zeta potential of -11.3 mV; The entrapment efficiency of 61.69%. Showed IC50 of liposomes extract and extract solution are respectively 11,997 ppm and 27,488 ppm. This result showed that the extract of bitter herbs encapsulation with liposomes give effect to the antiploriferative activity of T47D breast cancer cells. ;A Sambiloto?s Plant (Andrographis paniculata Nees) has many benefits in the medicals treatment, one of them as an anticancer drug. Liposomes are one of the development of drug delivery systems that have been studied can be used as carriers of drugs, proteins, and other molecular substances. This research aims to develop drugs with a basis of extract of bitter herbs are encapsulated with liposomes for comparison their antiproliferation activity against T47D breast cancer cells. The method which used in the manufacture of liposomes is thin layer hydration method. Reduction of particle size liposomes is done by extrusion. Evaluations were performed in this study is the particle size distribution and zeta potential by DLS, entrapment efficiency by dialisis, morphology size by TEM. Antiproliferation cancer cells test using MTT method. Results obtained at the TEM depicting the model OLV liposomes with a size of about ±50 nm; liposome particle size distribution of 441 and 45.3 nm, and zeta potential of -11.3 mV; The entrapment efficiency of 61.69%. Showed IC50 of liposomes extract and extract solution are respectively 11,997 ppm and 27,488 ppm. This result showed that the extract of bitter herbs encapsulation with liposomes give effect to the antiploriferative activity of T47D breast cancer cells. , A Sambiloto’s Plant (Andrographis paniculata Nees) has many benefits in the medicals treatment, one of them as an anticancer drug. Liposomes are one of the development of drug delivery systems that have been studied can be used as carriers of drugs, proteins, and other molecular substances. This research aims to develop drugs with a basis of extract of bitter herbs are encapsulated with liposomes for comparison their antiproliferation activity against T47D breast cancer cells. The method which used in the manufacture of liposomes is thin layer hydration method. Reduction of particle size liposomes is done by extrusion. Evaluations were performed in this study is the particle size distribution and zeta potential by DLS, entrapment efficiency by dialisis, morphology size by TEM. Antiproliferation cancer cells test using MTT method. Results obtained at the TEM depicting the model OLV liposomes with a size of about ±50 nm; liposome particle size distribution of 441 and 45.3 nm, and zeta potential of -11.3 mV; The entrapment efficiency of 61.69%. Showed IC50 of liposomes extract and extract solution are respectively 11,997 ppm and 27,488 ppm. This result showed that the extract of bitter herbs encapsulation with liposomes give effect to the antiploriferative activity of T47D breast cancer cells. ]