Hasil Pencarian  ::  Simpan CSV :: Kembali

"Telah dilakukan penelitian mengenai aktivitas antagonistis 52 isolat actinomycetes terhadap 2 isolat Ganoderma TB3 dan TB4. Isolat actinomycetes tersebut berasal dari pesisir Pulau Pramuka, Kepulauan Seribu, DKI Jakarta. Isolat Ganoderma berasal dari hutan kota Wales Timur serta kawasan hijau seberang Stadion Universitas Indonesia, Depok. Penapisan aktivitas antagonistis dilakukan dengan metode plug dan metode cross streak. Hasil penapisan diperoleh satu isolat actinomycetes SM 12 yang menghambat pertumbuhan kedua isolat Ganoderma. Uji lanjutan untuk mengetahui aktivitas antibiosis isolat SM 12 dilakukan dengan mencampurkan filtrat medium pertumbuhan SM 12 terhadap medium pertumbuhan Ganoderma TB3 dan TB4. Uji filtrat medium pertumbuhan pada hari ke-13 dan hari ke-16 inkubasi menunjukkan penghambatan pertumbuhan Ganoderma TB3 dan TB4. Sementara itu, aktivitas antagonistis dalam medium serbuk gergaji (bag log) tidak menunjukkan adanya hambatan pertumbuhan GanodermaTB3 dan TB4. ......Research about antagonistic activity of 52 isolates of actinomycetes against 2 isolates of Ganoderma TB3 and TB4 has been carried out. These actinomycetes were obtained from the of Pramuka Island Coast, Kepulauan Seribu, DKI Jakarta. Ganoderma isolates were obtained from Hutan Kota Wales Timur and green areas opposite Stadion Universitas Indonesia, Depok. Screening of antagonistic activity was carried out by plug method and cross streak method. The results of screening showed one actinomycetes isolate, SM 12, which able to inhibit two Ganoderma isolates. Further test to determine the antibiosis activity of SM 12 was carried out by mixing the growth filtrate of SM 12 on the growth media of Ganoderma TB3 and TB4. The test of the growth filtrate after 13 and 16 days of incubation showed inhibition of the growth of Ganoderma TB3 and TB4. Meanwhile, the antagonistic activity test on the sawdust media (bag log) did not show any inhibition of the growth of Ganoderma TB3 and TB4."

"ABSTRAKPenelitian tentang potensi antagonis bakteri Gram-positif Bacillus siamensis LDR terhadap pertumbuhan 2 isolat Asp. niger ABP dan ART memiliki selesai. Uji antagonis dilakukan dengan menggunakan metode kultur ganda yang berbeda, yaitu: kultur cakram ganda dan kultur piring tuang. Hasil pengujian pada metode kultur cakram ganda menunjukkan bahwa B. siamensis LDR mampu menghambat pertumbuhan diameter Asp. niger ART (44,83%) dan ABP (39,95%). Sedangkan pada metode pelat tuang, kultur, B. siamensis LDR mampu menghambat pertumbuhan Asp. niger ART (87,98%) dan ABP (92,43%). Fermentasi senyawa antijamur dari B. siamensis telah dilakukandengan metode fermentasi menggunakan media Potato Dextrose Broth untuk inkubasi 10, 12 dan 14 hari. Filtrat hasil fermentasi digunakan sebagai pelarut untuk medium medium PDA dan PDB dalam uji antibiotik. Uji antibiotik menunjukkan bahwa efektivitas terbesardiperoleh dari filtrat fermentasi 14 hari. Hasil pengujian pada media PDA berdasarkan diameter pertumbuhan koloni menunjukkan terhambatnya pertumbuhan Asp. niger ART (74,53%) dan ABP (62,13%). Sedangkan penghambatan pertumbuhan pada medium PDB yang ditentukan berdasarkan produksi biomassa menunjukkan penurunan sebesar 79,80% pada Asp. niger ART dan 51,22% pada Asp. niger ABP versus kontrol. Uji antibiosis dengan metode difusi cakram pada waktu inkubasi media fermentasi hari ke 14 juga selesai. Hasil pengujian pada metode difusi cakram menunjukkan bahwa penghambatan pertumbuhan Asp. niger ART dan ABP pada konsentrasi 2.500 ppmhingga 20.000 ppm.ABSTRACTResearch on the potential antagonist of Gram-positive bacteria Bacillus siamensis LDR to the growth of 2 isolates of Asp. niger ABP and ART have finished. The antagonist test was carried out using different multiple culture methods, namely: double disc culture and pour plate culture. The test results on the double disc culture method showed that B. siamensis LDR was able to inhibit the growth of Asp diameter. niger ART (44.83%) and ABP (39.95%). While in the pour plate method, culture, B. siamensis LDR was able to inhibit the growth of Asp. niger ART (87.98%) and ABP (92.43%). Fermentation of antifungal compounds from B. siamensis has been carried outby fermentation method using Potato Dextrose Broth media for incubation 10, 12 and 14 days. The fermented filtrate was used as a solvent for PDA and PDB medium in the antibiotic test. Antibiotic tests show that the greatest effectiveness obtained from 14 days fermentation filtrate. The test results on PDA media based on the diameter of the colony growth showed inhibition of Asp growth. niger ART (74.53%) and ABP (62.13%). Meanwhile, the inhibition of growth in the GDP medium which was determined based on biomass production showed a decrease of 79.80% in Asp. niger ART and 51.22% on Asp. niger ABP versus control. Antibiosis test using the disc diffusion method at the incubation time of the 14th day of fermentation media was also completed. The test results on the disc diffusion method showed that the inhibition of the growth of Asp. niger ART and ABP at a concentration of 2,500 ppmup to 20,000 ppm."

"

Bacillus spp. berpotensi sebagai agen biokontrol untuk meminimalisir kontaminasi mikroorganisme. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas antagonistis sel dan filtrat Bacillus siamensis LDR terhadap beberapa spesies kapang. Purifikasi dan karakterisasi morfologi dilakukan pada medium PDA. Uji aktivitas antagonistis dilakukan dengan metode dual kultur dan uji antibiosis dilakukan dengan menggunakan filtrat fermentasi B. siamensis LDR yang diperoleh dari panen hari ke-12 dan ke-14. Persentase inhibisi ditentukan berdasarkan perhitungan pertumbuhan radial dan biomassa kapang yang diberi perlakuan, dibandingkan dengan kontrol. Hasil uji antagonistis menunjukkan bahwa Fusarium oxysporum, Aspergillus flavus AHM, dan Aspergillus clavatus ABH berhasil dihambat sebesar 35,92%, 42.75%, 27.18% pada teknik dual disc dan sebesar 92.94%, 87.15%, 85.45% pada teknik pour plate disc. Hasil uji antibiosis menunjukkan inhibisi F. oxysporum, A. flavus AHM, dan A. clavatus ABH lebih tinggi pada filtrat 14 hari, yaitu 41.84—41,94%, 34,83—36,04%, 63.27—63,81% pada medium PDA dan 53,15—76,37%, -151— -11.01, 88.87—90.36% pada medium PDB. Sel dan filtrat B. siamensis LDR memiliki aktivitas antagonistis terhadap Fusarium oxysporum, Aspergillus flavus AHM, dan Aspergillus clavatus ABH dengan ketidakstabilan aktivitas antibiosis terhadap A. flavus AHM, secara in vitro.

 

---

Bacillus spp. are potential biocontrol agent to reduce crop contamination by microorganism. Aim of this research is to screen antagonistic activity of B. siamensis LDR cells and filtrate against few species of filamentous fungi. Purification and morphological characterization were done on PDA. Screening of antagonistic activity was done by antagonistic assay using dual culture method and antibiosis assay using 12 and 14 days fermentation filtrate of B. siamensis LDR. Percentage of inhibition were determined by comparing radial growth and biomass of treated fungi to untreated fungi as control. Result of antagonistic assay showed Fusarium oxysporum, Aspergillus flavus AHM, and Aspergillus clavatus ABH were inhibited for 35,92%, 42.75%, 27.18% by using dual disc technique and 92.94%, 87.15%, 85.45% by using pour plate disc technique, respectively. Result of antibiosis assay showed the highest inhibition activity against F. oxysporum, A. flavus AHM, A. clavatus ABH was found in 14 days fermentation filtrate, which were 41.84—41,94%, 34,83—36,04%, 63.27—63,81% on PDA and 53,15—76,37%, -151— -11.01, 88.87—90.36% on PDB, respectively. Therefore, B. siamensis LDR cell and filtrate has antagonistic activity against Fusarium oxysporum, Aspergillus flavus AHM and Aspergillus clavatus ABH, despite of unstable antibiosis activity against A. flavus AHM, in vitro.

 

"