Hasil Pencarian  ::  Simpan CSV :: Kembali

"ABSTRAK Minyak kemiri dapat diperoleh dari daging buah biji kemiri (Aieuritesmoluccana) dengan cara ekstraksi sinambung menggunakan peralatanekstraksi soxhlet dan pelarut yang digunakan adalah n-heksana sebesar45,47% dari berat serbuk kering biji kemiri.Dari penelitian ini dapat ditentukan sifat fisiko-kimia dari minyak bijikemiri. Untuk analisis dengan Gas Chromatography (GC), Jarutan minyakditransesterifikasi dengan metanoi-KOH dan Japisan n-heksana diinjeksikanke dalam peralatan GC yang dilengkapi dengan kolom kapiler AT-1000,dapat ditentukan komposisi asam l'emak penyusun trigliserida dari minyak bijikemiri, terdiri dari: asam kaprat (0,202%), asam palmitat (6,32%), asamstearat (2,31%), asam oleat(26,93%), asam linoleat (38,52%), dan asam,linolenat (25,25%)."

"Minyak goreng menjadi bahan yang umum digunakan daiam prosespengoiahan berbagai jenis makanan, karena praktis sekaiigus makananmenjadi mudah dlcerna dan memberikan rasa gurih bagi makanan yangdigoreng tersebut. Peneiitian ini bertujuan untuk memanfaatkan minyakgoreng bekas pakai dari restoran siap saji, yaitu sebagai bahan bakupembuatan amida asam iemak. Awainya, diiakukan penentuan sifat fisikokimiadari minyak goreng belum pakai dan bekas pakai, hasilnyamenunjukkan bahwa kualitas minyak goreng bekas pakai dari restoran siapsaji masih cukup baik. Pada penentuan komposisi asam lemak penyusuntrigiiserida dengan kromatografi gas, hasilnya menunjukkan bahwa minyakgoreng yang digunakan pada peneiitian ini adalah benar minyak sawit, sertakandungan terbesarnya adalah asam cleat (50,74%) dan asam paimitat(27,13%). Kemudian diiakukan sintesis amida asam lemak dari minyakgoreng sawit bekas pakai, dengan cara menghidrolisis dahulu trigiiserida dariminyak sehingga menghasilkan asam lemak sebanyak 94,73% dengan titikleieh 36,8-37,6X. Kemudian melalui amonolisis klorida asam, diperolehamida asam iemak sebanyak 81,12% dengan titik ieleh 97,2-99,4°C."

"[ABSTRAKMinyak ikan dikenal sebagai sumber polyunsaturated fatty acids (PUFA) yang baik dan digunakan untuk tujuan farmasetika dan suplemen pangan secara luas. Dalam penelitian ini, bahan baku ikan sidat (Anguilla bicolor bicolor) dikarakterisasi dan minyak ikan diekstraksi dari tulang ikan sidat dengan metode Bligh & Dyer dan wet rendering. Komposisi asam lemak dari minyaknya dianalisa dan dikuantifikasi menggunakan kromatografi gas. Hasil yang diperoleh menunjukkan, total by-product pengolahan ikan sidat (Anguilla bicolor bicolor) mencapai 26,38%, kandungan lemak tulang ikan sidat 17,33 ± 0,58 g/100 g. Rendemen minyak ikan sidat yang diekstraksi dengan metode Bligh dan Dyer adalah 17,12%. Berdasarkan hasil analisis asam lemak minyak ikan sidat, diperoleh kandungan SFA 19,87%, MUFA 25,84%, PUFA 13,84%. Komposisi asam lemak utama minyak ikan sidat adalah asam palmitat 13,58%, asam oleat 20,94%, asam linoleat 4,01%, EPA 1,57% dan DHA 4,84%. Rendemen tertinggi ekstraksi minyak ikan dari tulang ikan sidat menggunakan metode wet rendering adalah sebesar 6,95% yang didapat pada pada suhu 80ºC, waktu perebusan 60 menit. Penambahan waktu perebusan menjadi 90 menit pada suhu yang sama, tidak berpengaruh nyata terhadap persentase rendemen. Kondisi ekstraksi metode wet rendering terbaik berdasarkan persentase rendemen dan nilai hasil uji mutu dari minyak ikan adalah suhu perebusan 60°C, waktu perebusan 60 menit dengan persentase rendemen sebesar 5,53%, asam lemak bebas 0,47% , bilangan asam 9.277,55 mg KOH/kg, bilangan peroksida 38,35 meq/kg, bilangan anisidin 25,84 meq/kg dan total oksidasi 102,55 meq/kg. Hanya kandungan asam lemak bebas saja yang mempunyai nilai sesuai standar yang diperbolehkan.

---

ABSTRACTFish oils have been recognized as good sources of polyunsaturated fatty acids (PUFA) which are widely used for pharmaceutical purposes and as food supplements. In this study, eels (Angguilla bicolor bicolor) as a raw material was characterized and fish oil from eels bone were extracted using Bligh & Dyer and wet rendering method. The fatty acid composition of the oil was analyzed and quantified using gas chromatography. Results showed that the total yield of by-product of eel reached 26,38 %, the lipid content of eels bone was 17.33 ± 0.58 g/100 g. Yield of eel bone oil extracted by Bligh and Dyer method was 17.12%. In the fatty acid analysis of eel bone oil, it was discovered that SFA was 19.87%, MUFA was 25.84%, and PUFA was 13.84%. The Composition of major fatty acids in the oil from the bone were palmitic acid (13.58%), oleic acid (20.94%), linoleic acid (4.01%), EPA (1.57%), and DHA (4.84%). The highest yield of fish oil from eel bone with wet rendeering exctraction method was 6.95% on temperatur 80ºC and boiling time 60 minutes. At the same temperatur, an additional time to 90 minutes was not significantly different to percentage of yield. The best condition for wet rendering exctraction method is boiling temperature 60°C, boiling time 60 minute with value of yield, free fatty acid, acid value, peroxide value, anisidin value and total oxidation is 5,53%, 0,47%, 9.277,55 mg KOH/kg, 38,35 meq/kg, 25,84 meq/kg and 102,55 meq/kg respectively. Result showed that only free fatty acid value which meet the standar requirement.;Fish oils have been recognized as good sources of polyunsaturated fatty acids (PUFA) which are widely used for pharmaceutical purposes and as food supplements. In this study, eels (Angguilla bicolor bicolor) as a raw material was characterized and fish oil from eels bone were extracted using Bligh & Dyer and wet rendering method. The fatty acid composition of the oil was analyzed and quantified using gas chromatography. Results showed that the total yield of by-product of eel reached 26,38 %, the lipid content of eels bone was 17.33 ± 0.58 g/100 g. Yield of eel bone oil extracted by Bligh and Dyer method was 17.12%. In the fatty acid analysis of eel bone oil, it was discovered that SFA was 19.87%, MUFA was 25.84%, and PUFA was 13.84%. The Composition of major fatty acids in the oil from the bone were palmitic acid (13.58%), oleic acid (20.94%), linoleic acid (4.01%), EPA (1.57%), and DHA (4.84%). The highest yield of fish oil from eel bone with wet rendeering exctraction method was 6.95% on temperatur 80ºC and boiling time 60 minutes. At the same temperatur, an additional time to 90 minutes was not significantly different to percentage of yield. The best condition for wet rendering exctraction method is boiling temperature 60°C, boiling time 60 minute with value of yield, free fatty acid, acid value, peroxide value, anisidin value and total oxidation is 5,53%, 0,47%, 9.277,55 mg KOH/kg, 38,35 meq/kg, 25,84 meq/kg and 102,55 meq/kg respectively. Result showed that only free fatty acid value which meet the standar requirement.;Fish oils have been recognized as good sources of polyunsaturated fatty acids (PUFA) which are widely used for pharmaceutical purposes and as food supplements. In this study, eels (Angguilla bicolor bicolor) as a raw material was characterized and fish oil from eels bone were extracted using Bligh & Dyer and wet rendering method. The fatty acid composition of the oil was analyzed and quantified using gas chromatography. Results showed that the total yield of by-product of eel reached 26,38 %, the lipid content of eels bone was 17.33 ± 0.58 g/100 g. Yield of eel bone oil extracted by Bligh and Dyer method was 17.12%. In the fatty acid analysis of eel bone oil, it was discovered that SFA was 19.87%, MUFA was 25.84%, and PUFA was 13.84%. The Composition of major fatty acids in the oil from the bone were palmitic acid (13.58%), oleic acid (20.94%), linoleic acid (4.01%), EPA (1.57%), and DHA (4.84%). The highest yield of fish oil from eel bone with wet rendeering exctraction method was 6.95% on temperatur 80ºC and boiling time 60 minutes. At the same temperatur, an additional time to 90 minutes was not significantly different to percentage of yield. The best condition for wet rendering exctraction method is boiling temperature 60°C, boiling time 60 minute with value of yield, free fatty acid, acid value, peroxide value, anisidin value and total oxidation is 5,53%, 0,47%, 9.277,55 mg KOH/kg, 38,35 meq/kg, 25,84 meq/kg and 102,55 meq/kg respectively. Result showed that only free fatty acid value which meet the standar requirement., Fish oils have been recognized as good sources of polyunsaturated fatty acids (PUFA) which are widely used for pharmaceutical purposes and as food supplements. In this study, eels (Angguilla bicolor bicolor) as a raw material was characterized and fish oil from eels bone were extracted using Bligh & Dyer and wet rendering method. The fatty acid composition of the oil was analyzed and quantified using gas chromatography. Results showed that the total yield of by-product of eel reached 26,38 %, the lipid content of eels bone was 17.33 ± 0.58 g/100 g. Yield of eel bone oil extracted by Bligh and Dyer method was 17.12%. In the fatty acid analysis of eel bone oil, it was discovered that SFA was 19.87%, MUFA was 25.84%, and PUFA was 13.84%. The Composition of major fatty acids in the oil from the bone were palmitic acid (13.58%), oleic acid (20.94%), linoleic acid (4.01%), EPA (1.57%), and DHA (4.84%). The highest yield of fish oil from eel bone with wet rendeering exctraction method was 6.95% on temperatur 80ºC and boiling time 60 minutes. At the same temperatur, an additional time to 90 minutes was not significantly different to percentage of yield. The best condition for wet rendering exctraction method is boiling temperature 60°C, boiling time 60 minute with value of yield, free fatty acid, acid value, peroxide value, anisidin value and total oxidation is 5,53%, 0,47%, 9.277,55 mg KOH/kg, 38,35 meq/kg, 25,84 meq/kg and 102,55 meq/kg respectively. Result showed that only free fatty acid value which meet the standar requirement.]"

"Sintesis senyawa ester gliserol asam lemak hasil hidrolisis minyak sawit maupun palmitat dapat dilakukan secara enzimatik menggunakan lipase Candida rugosa dalam pelarut n-heksana. Reaksi esterifikasi dilakukan dengan menggunakan variasi perbandingan mmol antara asam lemak dengan gliserol 1:1; 1:2; 1:3; dan 1:4 mmol agar produk yang terbentuk mono-digliserida. Hasil FTIR ester gliserol asam lemak menunjukkan adanya puncak serapan gugus C=O ester pada bilangan gelombang 1741 cm-1 pada ester gliserol asam lemak hasil hidrolisis minyak sawit dan 1736 cm-1 ester gliserol palmitat. Ester yang dihasilkan diuji kemampuannya sebagai emulsifier menggunakan campuran minyak dan air.Hasil pengujian menunjukkan bahwa ester gliserol asam lemak hasil hidrolisis minyak sawit maupun palmitat dapat berperan sebagai emulsifier tipe minyak dalam air. Emulsi yang terbentuk stabil hingga 24 jam. Pada ester yang terbentuk juga dilakukan uji antimikroba menggunakan bakteri Propionibacterium acnes dan Staphylococcus epidermidis. Hasil penelitian menunjukkan bahwa ester gliserol asam lemak hasil hidrolisis maupun ester gliserol palmitat tidak memiliki aktivitas antibakteri tehadap Propionibacterium acnes dan Staphylococcus epidermidis.

---

Synthesis of glycerol ester hydrolized palm oil fatty acid and palmitic acid was conducted enzymatically using Candida rugosa in n hexane solvent. Esterification reaction using mmol ratio between fatty acids and glycerol were varied on 1 1 1 2 1 3 and 1 4 in order for the product to be formed mono diglyceride. glycerol ester hydrolized palm oil fatty acid and palmitic acid were characterized using FT IR. The results of FT IR analysis showed absorp tion peak that were related to the stretching of C O ester group at 1741 cm 1 for glycerol ester hydrolized palm oil fatty acid and at 1736 cm 1 for glycerol ester of palmitic acid. Esters were tested for their ability as emulsifiers using a mixture of oil and water.

The test results show that glycerol ester hydrolyzed palm oil and glycerol ester of palmitic acid can act as emulsifier type oil in water. The emulsion is stable for up to 24 hours. In the esters also performed an antimicrobial test using bacteria Propionibacterium acnes and Staphylococcus epidermidis. The results showed that glycerol ester hydrolyzed palm oil and glycerol ester of palmitic acid showed no antibacterial activity against Propionibacterium acnes and Staphylococcus epidermidis."

"Sintesis ester asam lemak karbohidrat pada penelitian ini dilakukan melalui reaksi esterifikasi antara glukosa dengan asam lemak hasil hidrolisis minyak kelapa. Reaksi esterifikasi dilakukan secara enzimatis menggunakan lipase Candida rugosa dengan pelarut organik (n-heksana) serta kandungan air yang relatif sedikit. Produk hasil sintesis diuji dengan uji emulsi dan diketahui memiliki sifat sebagai emulsifier. Hasil identifikasi produk menggunakan FT-IR menunjukkan serapan gugus ester pada bilangan gelombang 1737 cm-1. Pada imobilisasi lipase dalam matriks gel Ca-alginat, diperoleh kondisi optimum pada konsentrasi alginat 1% dan menghasilkan efisiensi imobilisasi sebesar 61,66%. Optimasi kondisi esterifikasi dengan lipase imobil dilakukan pada beberapa parameter seperti variasi suhu inkubasi, rasio substrat, waktu inkubasi, dan berat molecular sieve. Kondisi optimum reaksi esterifikasi diperoleh pada suhu 35o C, rasio mol asam lemak dengan glukosa 60:1, waktu inkubasi 16 jam, dan tanpa penambahan molecular sieve, dengan persen konversi sebesar 4,10% ......In this study, the synthesis of fatty acid ? carbohydrate esters used glucose (G) and coconut oil fatty acid (FA). The esterification reaction was carried out enzymatically using Candida rugosa lipase in the present of organic solvent (nhexane). The synthesized product was then examined by simple emulsion test and was proved to be an emulsifier. The characterization of synthesized product with FT-IR showed that product exhibit the absorbtion of ester functional group at 1737 cm-1. Lipase immobilization on Ca-alginate gel beads was also carried out to ease the separation of the enzyme from the system. The optimum condition for immobilization is by using Na-alginate 1%, resulting 61,66% immobilization yield. Some parameters in the esterification reaction such as temperature, the molar ratio between glucose and fatty acid, time for incubation, and the weight of molecular sieve were also optimized. The optimum conditions for esterification using immobilized lipase on Ca-alginate were at 35o C, the molar ratio 1:60 (G/FA), 16 hours incubation time and 0 gr molecular sieve. The acid convertion at optimum conditions was up to 4,10%."

"ABSTRAKEster sukrosa adalah emulsifier ramah lingkungan, yang dapat disintesis melalui reaksi esterifikasi antara sukrosa dengan asam lemak secara kimiawi maupun secara enzimatik. Penelitian sebelumnya menunjukkan bahwa sintesis ester sukrosa secara enzimatik dapat dilakukan dengan menggunakan lipase dalam kondisi terdapat pelarut organik dan kandungan air yang sedikit. Pada penelitian ini, ester sukrosa disintesis melalui reaksi esterifikasi antara sukrosa dengan asam lemak hasil hidrolisis minyak sawit menggunakan lipase Candida rugosa dengan pelarut heksana. Optimasi reaksi esterifikasi dilakukan pada beberapa parameter seperti waktu inkubasi, suhu, dan rasio substrat untuk menghasilkan persentase konversi tertinggi. Kondisi optimum diperoleh pada waktu inkubasi 12 jam, suhu reaksi 30 0C, dan rasio mol asam lemak dengan sukrosa 64:1. Identifikasi produk menggunakan instrumen FT-IR memberikan serapan gugus ester pada bilangan gelombang 1739 cm-1. Pada uji emulsi sederhana, produk yang dihasilkan dapat bertindak sebagai emulsifier. Pada hasil analisis menggunakan HPLC, diperoleh puncak monoester sukrosa pada waktu retensi 5,39; 5,71; 5,98; 6,11; 6,42 menit, puncak diester sukrosa pada waktu retensi 7,49 dan 7,85 menit, puncak triester sukrosa pada waktu retensi 12,93 menit, dan puncak tetraester sukrosa pada waktu retensi 18,12 menit. Analisis kuantitatif HPLC menunjukkan bahwa komposisi tertinggi yang terbentuk merupakan ester sukrosa dengan diester sukrosa, yaitu sebesar 77,22%.

---

ABSTRACTSucrose esters are biodegradable emulsifiers, that may be synthesized by numerous methods which involve the use of either chemical or enzymatic esterification between fatty acids and sucrose. A previous study showed that enzymatic synthetis of sucrose ester could be carried out in organic media and minimum amount of water with lipase as biocatalyst. In this study, sucrose esters were synthesized by enzymatic esterification between hydrolyzed palm oil fatty acids and sucrose using lipase from Candida rugosa with hexane as solvent. Optimization of esterification reaction including incubation time, temperature reaction, and substrate molar ratio for a high conversion of sucrose ester. The optimum conditions were achieved at incubation time 12 h, temperature 30 0C, substrate fatty acid to sucrose molar ratio 64:1. Identification of products using FT-IR instrument gave an ester band at wave number 1739 cm-1. In a simple emulsion test, the synthesized product could be act as emulsifier. Analysis using HPLC gave sucrose monoester peak at the retention time of 5.39; 5.71; 5.98; 6.11; 6.42 minutes, sucrose diester peak at retention time 7.49 and 7.85 min, peak sucrose triester at retention time 12.93 minutes, and peak tetraester sucrose at retention time 18.12 minutes. Quantitative analysis using HPLC, the highest composition formed was diester sucrose, that is equal to 77.22%."

"Ester asam lemak kelapa sawit sukrosa dapat disintesis melalui reaksi esterifikasi enzimatis menggunakan lipase Candida rugosa. Pada penelitian ini, digunakan pelarut isoamil alkohol dan isobutanol. Reaksi esterifikasi menggunakan lipase Candida rugosa E.C.3.1.1.3 bebas maupun terimobilisasi pada nanopartikel Fe3O4-kitosan. Imobilisasi lipase Candida rugosa pada nanopartikel Fe3O4-kitosan menggunakan metode ikat silang dengan glutaraldehida sebagai agen pengikat silang. Terhadap hasil imobilisasi dilakukan uji aktivitas dan persen loading. Pada peggunaan enzim 200 ppm didapatkan persen loading imobilisasi lipase sebesar 31,4% dengan aktivitas hidrolisis lipase terimobilisasi sebesar 3,470 U/mL, aktivitas spesifik 0,991 U/mg, serta efisiensi imobilisasi sebesar 23,25 %. Sementara itu, pada penggunaan 350 ppm enzim didapatkan persen loading imobilisasi lipase sebesar 64,4 % dengan aktivitas hidrolisis lipase terimobilisasi sebesar 2,245 U/mL, aktivitas spesifik 0,64 U/mg, serta efisiensi imobilisasi sebesar 14,88%. Kondisi rasio substrat optimum diperoleh pada rasio 1 : 90 (sukrosa : asam lemak). Hasil uji emulsifer menunjukkan hasil positif. Pelarut terbaik untuk sintesis ester -sukrosa dengan lipase terimobilisasi adalah isobutanol.

---

Palm oil fatty acid-sucrose can be synthesized via enzymatic esterification using Candida rugosa lipase. The solvents used in this study were isoamyl alcohol and isobutanol. Esterification reaction was carried out enzymatically using free lipase and immobilized Candida rugosa lipase E.C. 3.1.1.3 on Fe3O4-chitosan nanoparticles. The immobilized lipase were examined to determine the loading percentage and the hydrolytic activity. The loading percentage obtained while using 200 ppm lipase for immobilization was 31,4%, with hydrolytic activity of immobilized lipase was 3,47 U/mL, the specific activity of 0,99 U/mg and the immobilization efficiency of 23,25%. Meanwhile by using 350 ppm lipase the loading percentage obtained was 64,4% with hydrolytic activity of 2,25 U/mL, the specific activity of 0,64 U/mg and the immobilization efficiency of 14,8 %. From this optimization study of esterification, the highest conversion came from sugar and fatty acid ratio 1 : 90. The esterification product gave positive test on emulsifier test. The best solvent to synthesize ester-sucrose using immobilized lipase is isobutanol."

"Ester asam lemak sakarida dapat diperoleh melalui reaksi esterifikasi antara maltosa dengan asam lemak minyak sawit menggunakan pelarut organik. Reaksi esterifikasi dilakukan secara enzimatik menggunakan katalis lipase Candida rugosa E.C.3.1.1.3 terimobilisasi pada nanopartikel Fe3O4-Kitosan, dengan pelarut isoamil alkohol dan iso butanol. Imobilisasi lipase Candida rugosa pada nanopartikel Fe3O4-Kitosan menggunakan metode ikat silang dengan glutaraldehida sebagai agen pengikat silang. Imobilisasi dilakukan menggunakan larutan lipase dengan konsentrasi awal enzim yaitu 200 dan 350 ppm. Variasi yang dilakukan menggunakan perbandingan rasio mol substrat gula: asam lemak, yaitu 1:30 ; 1:60 ; dan 1:90. Hasil yang lebih baik diperoleh menggunakan konsentrasi awal lipase 200 ppm, dengan nilai persen loading imobilisasi lipase yang diperoleh adalah 31,28 % dan aktivitas hidrolisis lipase terimobilisasi sebesar 2,88 U/mL dan aktivitas spesifiknya sebesar 1,23 U/mg. Hasil optimasi perbandingan rasio molar gula dan asam lemak menunjukkan kondisi optimum diperoleh pada rasio 1 : 30 menggunakan pelarut iso butanol, dengan % konversi 5,0 %. Hasil uji emulsifier menunjukkan bahwa produk esterifikasi dapat berfungsi sebagai emulsifier.

---

Sugar esters can be produced with esterification between maltose and palm oil fatty acid in organic solvents. Esterification reaction was carried out enzymatically using immobilized Candida rugosa lipase EC.3.1.1.3 on Fe3O4--chitosan nanoparticles, with isoamyl alcohol and isobutanol as the solvents. Candida rugosa lipase was immobilized on Fe3O4-chitosan nanoparticles using cross-linking method with glutaraldehyde as cross linker. Immobilization was carried out with two initial enzyme concentrations of 200 and 350 ppm. The variations of molar substrate ratio of sugar : fatty acid used in this study were 1:30, 1:60, and 1:90. The optimal results was obtained using initial concentration of 200 ppm lipase. The loading percentage of immobilized lipase was 31,28 % with hydrolytic activity of immobilized lipase was 2,88 U/mL and the specific activity was 1,23 U/mg. The highest % conversion in esterification obtained in this study was 5.0 %, using molar ratio 1:30 in iso bthanol as solvent. The ester product obtained was then examined by simple emulsion test and can be used as an emulsifying agent."