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"ABSTRAKIonizing radiation (IR) presents a risk to human health via DNA damage even when administered at low doses, such as those used in panoramic radiography. Objectives: This study used the comet assay to assess DNA damage in buccal mucosa cells consequent to X ray radiation from panoramic radiography. Methods: Twenty participants were recruited from among patients who underwent panoramic examinations at Prof. Soedomo Dental Hospital, Universitas Gadjah Mada, and divided into two groups of 10. Buccal mucosa cells were collected from all participants before exposure to IR and at 30 min or 24 h after exposure in groups 1 and 2, respectively, and subjected to a comet assay to assess DNA damage. Assay output images were analyzed using OpenComet software. Double-strand breaks (DSBs) were assessed by comparing the percentages of tail DNA in output images obtained before and after X ray exposure. Results: A statistically signifcant (p 0.014) increase in the percentage of tail DNA was observed at 30 min after exposure, but not at 24 h (p 0.29). Conclusion: Panoramic X ray radiation may induce DSBs in human buccal mucosal cells within 30 min after exposure. "

"ABSTRAKPenelitian ini bertujuan mengetahui perbedaan nilai mAgNOR antara kelompok perokok kretek dan bukan perokok dan mengetahui hubungan karakteristik perokok dengan nilai mAgNOR. Metode penelitian analitik komparatif dengan pendekatan potong lintang. Pengambilan spesimen menggunakan sikat oral pada mukosa bukal tampak normal dari 21 laki-laki usia 26-35 tahun pada masing-masing kelompok. Hasilnya, nilai mAgNOR pada kelompok perokok kretek (2,9543±0,35537) lebih tinggi daripada kelompok bukan perokok (2,6971±0,43732), secara statistik signifikan. Tidak terdapat hubungan antara nilai mAgNOR dengan karakteristik intensitas merokok, lama tahun kebiasaan merokok dan lama terpapar rokok. Terdapat hubungan antara mAgNOR dengan karakteristik lama menghabiskan 1 batang rokok (p=0,030, r=0,475).

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ABSTRACTThe aim of this study is to determine the difference between mAgNOR counts of kretek smokers and nonsmokers, and determine the relationship of smokers characterstics with mAgNOR counts. This comparative analytical research with cross sectional approach. Specimen using oral brush on the buccal mucosa appears normal of 21 men aged 26-35 years old in each group. As a result, mAgNOR counts of the smokers group (2,9543±0,35537) is higher than nonsmokers group (2,6971±0,43732), statistically significant. There was no correlation between mAgNOR counts with intensity of smoking, years and exposure time to kretek smoke. There was a correlation between mAgNOR counts with timing spend per smoke (p=0,030, r=0,475)."