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Aktivitas sitotoksik ekstrak etanol daun aglaia elliptica blume terhadap galur sel kanker serviks (HeLa)
"Cytotoxicity tests on ethanol extract of aglalia elliptica blume leaves and its fractions on cervical cencer cell line (HeLa) were carried out. Dried powder of Aglaia elliptica leaves was extracted using ethanol and then fractionated by n-hrxane, ethyl acetate and buthanol extraxt solvents. The cytotoxicity tets using MTT method (prolipheration inhibition tests on HeLa cell lines were conducted for ethanol extract, n-hexana ethyl acetate and buthanol fractions. The results showed that the ethanol extraxt of aglaia elliptica and its fractions have inhibition effect againts HeLa Cell line with IC50 of 282.44 ppm,177.64 ppm, 16.03 ppm,211.77 ppm, 181.33 ppm. 181.33 for ethanol extracts ,n-hexane(Heal extract , ethyl acceate, buthanol,and water fractions respecively."
Artikel Jurnal  Universitas Indonesia Library
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Susanto
Pengaruh Iradiasi Gamma Terhadap Kandungan Kuersetin dan Khasiatnya pada Ekstrak Etanol Bunga Rosela (Hibiscus sabdariffa L.) = The Effect of Gamma Irradiation on Quercetin and Efficacy in Ethanol Extract of Roselle (Hibiscus sabdariffa L.)
"Iradiasi gamma banyak digunakan oleh beberapa industri obat herbal sebagai metode pengawetan yang efisien dalam mengurangi kontaminasi mikroorganisme. Tujuan dari penelitian ini adalah untuk mengevaluasi pengaruh iradiasi gamma (0; 5; 7.5; and 10 kGy) pada ekstrak etanol H. sabdariffa (EEHS) terhadap uji total mikroorganisme, total kandungan fenol dan flavonoid, aktivitas antioksidan, TLC profiling, total kuersetin dan bobot molekul, aktivitas penghambatan terhadap enzim α-glukosidase dan in-vitro analisis terhadap galur sel kanker manusia (A-549,HUT-78, dan MCF-7). Iradiasi dosis 5 kGy menunjukkan bahwa tidak adanya kapang yang tumbuh dan terjadi penurunan jumlah total bakteri, lebih lanjut iradiasi pada dosis 10 kGy tidak terjadi pertumbuhan bakteri. Analisis total fenol, dan flavonoid, serta aktivitas antioksidan menunjukkan adanya penurunan sebesar 5-11% setelah diiradiasi pada dosis 5 kGy. Analisis profil TLC dan HPLC menunjukkan bahwa salah satu senyawa dalam EEHS adalah kuersetin yang ditunjukan dengan adanya [M+H]+ pada m/z 303,04 dari hasil analisis LC-Ms/Ms. EEHS juga memiliki penghambatan terhadap aktivitas enzim α-glukosidase dengan nilai penghambatan 4,75-7,55%. Uji aktivitas anti kanker terhadap galur sel kanker manusia menunjukkan bahwa EEHS memiliki kemampuan menginhibisi sel kanker sangat kuat dengan nilai IC50 < 20 µg/mL. Lebih lanjut, khasiat anti kanker paling kuat terhadap HUT-78, dengan nilai IC50 10,51 µg/mL, diikuti terhadap MCF-7 (IC50 13,39 µg/mL), dan  A-549 (IC50 14,19 µg/mL). Diketahui pula bahwa iradiasi dosis 5-10 kGy mampu menurunkan aktivitas anti kanker, namun penurunan tersebut tidak menghilangkan aktivitasnya yang ditandai nilai IC50 < 20 µg/mL. Berdasarkan data yang diperoleh, dapat disimpulkan bahwa iradiasi gamma dapat digunakan sebagai pengawetan pada ekstrak etanol H. sabdariffa Linn.
Gamma irradiation is widely used by many herbal medicine industries as an efficient preservative method in reducing microorganism contamination. The purpose of this study was to evaluate the effect of gamma irradiation (0; 5; 7.5; and 10 kGy) on the ethanol extract of H. sabdariffa (EEHS) toward the total microorganism test, total phenol and flavonoid contents, antioxidant activity, TLC profile, total quercetin and its molecular weight, inhibitory activity against α-glucosidase enzyme , as well as in-vitro bioassay against human cancer cell lines (A-549, HUT-78, and MCF-7). Irradiation at a dose of 5 kGy showed that no mold grew and there was a decrease in the total number of bacteria, moreover at a dose of 10 kGy there was no bacterial growth. Analysis of total phenols and flavonoids, as well as antioxidant activitiy showed a decrease of 5-11% after irradiation at a dose of 5 kGy. TLC and HPLC profile analysis showed that one of the compounds in the H. sabdariffa extract was quercetin which was indicated by the presence of [M+H]+ at m/z = 303.04 from the LC-MS/MS analysis. EEHS also had inhibitoty  activity against α-glucosidase enzyme with the inhibition value of 4.75-7.55%. Bioassay anticancer against human cancer cell lines showed that EEHS had a very strong ability to inhibit cancer cells with the IC50 value < 21 µg/mL. Furthermore, the anti-cancer properties were strongest against HUT-78 with the IC50 value of 10.51 µg/mL, followed by MCF-7 (IC50 13.39 µg/mL), and A-549 (IC50 14.19 µg/mL). It is also known that irradiation doses of 5-10 kGy could reduce anti-cancer activity, however the decrease did not eliminate its activity which was the IC50 values still lower than 20 µg/mL. Based on the data obtained, it can be concluded that gamma irradiation can be used as a preservative method for ethanol extract of H. sabdariffa Linn."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2023
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UI - Tesis Membership  Universitas Indonesia Library
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Azzahra Fauzia Hanaum
Studi Efektivitas BIX01294 dalam Menurunkan Ekspresi Gen EHMT2 pada Sel Kanker Paru A549 = Effectiveness Study of Inhibitor BIX01294 in Reducing the Expression of the EHMT2 Gene by using A549 Lung Cancer Cell Line
"Gen EHMT2 dapat meregulasi metilasi protein histon yang berdampak pada disregulasi epigenetik dan terganggunya proses transkripsi. Hal tersebut dapat menjadi faktor yang memengaruhi metastasis tumor pada kanker paru. Salah satu inhibitor spesifik yang dapat digunakan untuk menghambat aktivitas dan menurunkan ekspresi gen EHMT2 adalah BIX01294. Sejauh ini, konsentrasi inhibitor BIX01294 yang digunakan untuk penelitian ekspresi gen EHMT2 pada kanker paru berkisar pada 2–10 μM. Oleh karena itu, perlu dilakukan penelitian lanjutan terkait efektivitas inhibitor BIX01294 untuk menurunkan ekspresi gen EHMT2 dengan konsentrasi >10 μM. Penelitian ini bertujuan untuk mengetahui efektivitas dari inhibitor BIX01294 dalam menurunkan ekspresi gen EHMT2 pada sel kanker paru A549 melalui empat konsentrasi inhibitor yang berbeda, yakni 12,5 μM, 15 μM, 17,5 μM, dan 20 μM. Metode yang digunakan adalah RT-qPCR. Hasil penelitian menujukkan bahwa inhibitor BIX01294 dapat memengaruhi konfluensi dan viabilitas sel kanker paru A549. Sampel sel kanker paru A549 yang tidak diberi perlakuan dengan inhibitor BIX01294 memiliki konfluensi sel dan nilai viabilitas yang lebih tinggi dibandingkan dengan sampel seri inhibitor BIX01294. Selain itu, inhibitor BIX01294 juga dapat menurunkan ekspresi gen EHMT2 sel kanker paru A549 secara dose - dependant . Dengan demikian, dapat disimpulkan bahwa inhibitor BIX01294 dengan konsentrasi 12,5 μM; 15 μM; 17,5 μM; dan 20 μM dapat menurunkan ekspresi gen EHMT2 dan viabilitas pada sel kanker paru A549 secara dose-dependant.
The EHMT2 gene has the ability to control the methylation of histone proteins, which can lead to disruptions in epigenetic regulation and the transcription process. This can be a factor influencing tumor metastasis in lung cancer. A specific EHMT2 gene inhibitor, BIX01294, has been identified as an effective agent in inhibiting the activity of the EHMT2 gene. Previous research on lung cancer used BIX01294 concentrations between 2–10 μM. As a result, additional studies are needed to evaluate the efficacy of the BIX01294 inhibitor in reducing EHMT2 gene expression at concentrations greater than 10 μM. The objective of this study is to evaluate the potency of the BIX01294 inhibitor in reducing EHMT2 gene expression within A549 lung cancer cells at four different concentrations: 12.5 μM, 15 μM, 17.5 μM, and 20 μM. The method used in this study is RT-qPCR. The study’s findings reveal that the BIX01294 inhibitor can impact the confluence and viability of A549 lung cancer cells. The A549 lung cancer cell samples that were not subjected to the BIX01294 inhibitor exhibited higher cell confluence and viability values compared to those that were treated with the inhibitor. Furthermore, the BIX01294 inhibitor can also decrease the expression of the EHMT2 gene in A549 lung cancer cells in a dose-dependent manner. Therefore, it can be concluded that the BIX01294 inhibitor, at concentrations of 12.5 μM, 15 μM, 17.5 μM, and 20 μM, demonstrates a dose-dependent reduction in EHMT2 gene expression and the viability of A549 lung cancer cells."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2024
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UI - Skripsi Membership  Universitas Indonesia Library
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Megandhita Sharasti
Uji Fitokimia dan Efek Penghambatan Ekstrak Orange Cup Coral (Tubastraea coccinea) Terhadap Cell Line Kanker Paru A549 = Phytochemical Compound Assessment and Tumour Cell Inhibitory Effect of Orange Cup Coral (Tubastraea coccinea) against Lung Cancer Cell Line A549
"Latar belakang: Prevalensi kanker paru adalah salah satu yang tertinggi di Indonesia. Namun, resistensi dan penurunan efektivitas obat terus terjadi. Tingginya kekayaan alam di Indonesia membuka peluang untuk bahan alam dapat dimanfaatkan sebagai alternatif pengobatan kanker paru. Salah satunya adalah Tubastraea coccinea yang merupakan spons laut di daerah Indo-Pasifik yang berpotensi dikembangkan menjadi obat kanker.
Metode Sampel T. coccinea diekstraksi dengan teknik maserasi menggunakan pelarut etil asetat, etanol, dan n-heksana. Uji fitokimia ekstrak T. coccinea secara kualitatif dengan menguji tannin, saponin, flavonoid, triterpenoid, steroid, alkaloid, dan glikosida, kemudian dilakukan uji kromatografi lapis tipis. Uji sitotoksisitas ekstrak T. coccinea terhadap sel kanker paru A549 dilakukan dengan menggunakan metode MTT.
Hasil: Uji fitokimia ekstrak etanol, etil asetat, dan n-heksana T. coccinea positif mengandung flavonoid dan triterpenoid. Uji kromatografi lapis tipis menunjukkan ekstrak etanol T. coccinea memiliki komponen senyawa dengan Rf= 0,30 dan 0,85; ekstrak n-heksana T. coccinea memiliki komponen senyawa dengan Rf= 0,88 dan 0,97; ekstrak etil asetat T. coccinea memiliki Rf= 0,25; 0,67; 0,86. Uji MTT ekstrak etanol T. coccinea terhadap sel kanker paru A549 memiliki IC50 rata-rata 1,36 µg/ml, ekstrak dengan etil asetat memiliki IC50 sebesar 21,12 µg/ml, ekstrak dengan n-heksana memiliki IC50 sebesar 77,01 µg/ml.
Kesimpulan: Ketiga ekstrak T.coccinea positif mengandung flavonoid dan triterpenoid, ketiga ekstrak T. coccinea memiliki kemampuan sitotoksisitas terhadap sel paru A549. Ekstrak etanol T. coccinea menunjukkan aktivitas sitotoksik tertinggi terhadap sel paru A549 dan lebih baik dibandingkan dengan kontrol positif doxorubicin.
Introduction: Lung cancer prevalence in Indonesia is one of the highest. However, drug resistance and decline in drug effectivity is increasing. Indonesia’s natural diversity opens opportunities for natural ingredients to be used as an alternative treatment for lung cancer. One of them is Tubastraea coccinea which is a marine sponge spread across the Indo-Pacific region with a lot of pharmaceutical potentials.
Method Samples of T. coccinea were extracted by maceration technique and then dissolved with ethyl acetate, ethanol, and n-hexane. Phytochemical assessments were carried out qualitatively by assessing tannins, saponins, flavonoids, triterpenoids, steroids, alkaloids, and glycosides, and then assessed by Thin-Layer Chromatography. MTT assessment used for testing the extract’s cytotoxicity against cell line A549.
Result: The phytochemical assessment of T. coccinea n-hexane, ethanol, and ethyl acetate extract was positive for containing flavonoids and triterpenoids. The thin-layer chromatography test revealed that the T. coccinea ethanol extract had Rf= 0.30 and 0.85 ; T. coccinea n-hexane extract had Rf= 0.88 and 0.97; T. coccinea ethyl acetate extract had Rf= 0.25; 0.67; 0.86. The MTT assay of T. coccinea extract against cell line A549 for T. coccinea ethanol extract IC50=1.36 µg/ml, for T. coccinea ethyl acetate extract IC50=  21.12 µg/ml, for T. coccinea n-hexane extract IC50= 77.01 µg/ml.
Conclusion: ethanol, ethyl acetate, and n-hexane extract of T. coccinea were positive for flavonoids and triterpenoids, all of them also had cytotoxicity effect against cell line A549 with T. coccinea ethanol extract having the highest cytotoxic activity against cell line A549 and better than doxorubicin."
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2021
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UI - Skripsi Membership  Universitas Indonesia Library