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"The bioetanol development from biomass bases of lignocellulose like bagasse is one of alternative energy which has potential to be applied in Indonesia. Beside of raw material source that is so many in our country, the process is also environmentally friendly. Conversion of bagasse becomes etanol using Simultaneous Sacharification and Fermentation (SSH technology by cellulose and cellobiase enzyme had been done on this research. Sacharification process or hydrolysis process, cellulose enzyme will break cellulose polymer becomes glucose whereas cellobiose enzyme will break cellobiose becomes glucose.

Then, glucose through fermentation is changed to etanol by using yeast Saccharomyces cerevisiae. The variations include pH of system that is pH 4' ; 4,5 and 5, HCI addition low concentrated HCI at pH 5 with variation of concentration that is 0,5 % and I %, also variation of sample at pH 5 where bagasse without pretreatment is compared with bagasse which had been done pretreatment by using fungi Lentinus edodes for 4 weeks.

The result shows that the use of cellulose and cellobiase enzyme with system optimum condition pH 5 produce etanol concentration is higher than using only cellulose enzyme at the same pH condition. For substrate concentration 50 g/L, on the use of cellulose and cellobiase, the highest etanol concentration which is produced bagasse without pretreatment is 5,62 g/L or li,24 % from bagasse. On HCI addition, the highest etanol concentration is produced by concentration HCI i % with amount 6,52 g/L or 13,04 % from bagasse. With bagasse L. edodes and P. ostreatus 6 weelts, the highest etanol concentration that is 6 86 g/L and 6,50 g/L or 13, 72% and l2,99% from bagasse. It also shows that HCl addition low concentrated and pretreatment by white rot fungi L. edodes and P. ostreatus can increase the etanol quantity that is produced from bagasse conversion."

"Penggunaan bahan bakar fosil oleh manusia menimbulkan ancaman serius, yaitu jaminan ketersediaan bahan bakar fosil untuk beberapa dekade mendatang dan polusi akibat emisi pembakaran bahan bakar fosil ke lingkungan. Kesadaran terhadap ancaman tersebut telah mengintensifkan berbagai riset yang bertujuan menghasilkan sumber-sumber energi alternatif yang berkelanjutan dan lebih ramah lingkungan. Salah satu energi alternatif yang relatif murah ditinjau aspek produksinya dan relatif ramah lingkungan adalah pengembangan bioetanol dari limbah-limbah perkotaan yang mengandung banyak lignoselulosa seperti kertas (limbah kertas). Kertas mengandung sekitar 85% selulosa, 8% hemiselulosa, 5% lignin dan sisanya berupa senyawa abu. Pada penelitian ini telah dilakukan percobaan untuk membuat etanol dari limbah kertas dengan proses Sakarafikasi dan Fermentasi Serentak (SSF). Kombinasi enzim selulase + selobiase dan yeast Saccharomyces cerevisiae digunakan untuk hidrolisis dan fermentasi dalam proses SSF tersebut. Variasi kertas yang digunakan adalah koran, HVS tinta, HVS kosong dengan masing-masing pH 5. sedangkan variasi enzim yang digunakan adalah 0,2 gr ;0,3 gr ;0,5 gr. Proses SSF dilakukan dengan waktu inkubasi selama 6,12, 24, 36, 48, 72, 96 jam. Perlakuan dengan enzim 0,2 gr , 0,3 gr , 0,5 gr yang masing - masing pH 5 menghasilkan konsentrasi etanol tertinggi berturut-turut 831,6 ppm pada HVS kosong, 831,56 ppm pada HVS kosong, 1531,33 ppm pada HVS tinta. Kandungan etanol yang terbaik dihasilkan oleh HVS tinta pada 0,5 gr enzim dengan kadarnya sebesar 5,17 %. ......The use of fossil fuel by human can cause seriously threat likes available of fossil fuel for further decade and pollution to the environment by emission from fossil fuel. Consider of that threats, caused intensify many researches to produce sustainability alternative energy resources and more environments friendly. One of the sustainable alternative energy is relatively cheap production and environment friendly was development bio ethanol from waste residue. It does contain many lignocelluloses like paper (waste paper residue). Paper contains approximately 85% cellulose, 8% hemicelluloses, 5% lignin and the other such as ash compound. This research deals with ethanol production from sugar cane paper using Simultaneous Saccharification and Fermentation (SSF) process. Cellulase and Cellobiose combination enzyme and Saccharomyces cerevisiae was used to hydrolyse and fermentation in SSF process. Variation of waste paper was used such as newspaper, ink HVS, and blank HVS. Which each other used pH 5, with it does used enzyme like 0,2 gr ; 0,3 gr ; 0,5 gr . The SSF process was done with 24, 48, 72, and 96 hour's incubation time for fermentation. As a treatment enzyme 0,2 gr ; 0,3 gr ; 0,5 gr which its for pH 5 can be produce merely following is 831,6 ppm blank HVS, 831,56 ppm blank HVS , 1531,33 ppm pada ink HVS. The best result from them is ink HVS for 0,5 enzym, with percentage is 5,17%"