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"ABSTRAKPrevious study shows that tryptone-yeast extract-cystine-sucrose is choice of the culture media for isolating and growing S. mutans serotype c, but the affectiveness in stepping up the recovery growth of the bacteria has never been compared with that of the blood agar added to yeast extract and sucrose. This study was come to find out the growth of S. mutans serotype c with blood agar-yeast extract-sucrose. S. mutans INA99 serotype c is identified, sliced, and cultured into blood agar-yeast extract-sucrose, brucella agar blood-yeast extract-sucrose, blood agar and tryptone-yeast extract-cystine-sucrose, each 4 slices. The average amount of blood agar colonies that grows after an incubation of 2x24 hours in an anaerob condition of 37 C, diluted by 40 time is 4.890. In blood agar-yeast extract-sucrose the average colony is 145.600, diluted 1.600 time. In brucella agar blood-yeast extract-sucrose, the average colony is 103.500, diluted 40 times. In tryptone-yeast extract-cystine-sucrose the average colony is 2.430, diluted 40 time. Statistical result shows that, there are significant difference (p=0.000) between the total colonies of S. mutans INA99 serotype c which grows on the different types of culture media. The largest amount of colony is present on blood agar-yeast extract-sucrose, then followed by brucella agar blood-yeast extract-sucrose, blood agar and tryptone-yeast extract-cystine-sucrose. Conclusion: blood agar which added yeast extract and sucrose is the best culture media for S. mutans INA99 serotype c growth."

"This review article gives a brief history of the classical experiments that led to the development of the embryo culture medium and in vitro embryo culture. It proposes that, in view of the outstanding and significant pioneering contributions of Wesley Kingston Whitten to the development of embryo culture medium, he be considered the “Father of Embryo Culture Medium”. Furthermore, it describes the nutritional requirements of early embryos and how these requirements with specific references to carbohydrates, amino acids, phosphates, growth factors, etc, have been utilized to formulate increasingly more complex embryo culture media. This has led to the development of progressively more efficacious embryo culture media including the formulation of completely defined and synthetic protein-free embryo culture medium. The review also describes physical factors, growth factors, insemination methods for the fertilization of oocytes and culture methods affecting embryo growth, development, metabolism, oxygen embryotoxicity and survival. In procedural terms, the review also summarizes the evolution of embryo culture techniques from tube culture to, microdrop culture under oil to co-culture to ultra microdrop culture techniques. It includes techniques of in vitro maturation and for the selection of potentially viable embryos of various developmental stages. "

"Music is often our companion when dealing with the incomprehensibility of loss. This edited collection speaks to the multifarious and complex ways in which music accompanies, supplements, and complements aspects of death and dying, whether this is the death of a loved one, or a celebrity from popular culture."

"Reality television is shown worldwide, features people from all walks of life and covers everything from romance to religion. It has not only changed television, but every other area of the media. So why has reality TV become such a huge phenomenon, and what is its future in an age of streaming and social media?"

"Tulisan ini bertujuan mengungkap kondisi perempuan indonesia dalam kelompok ethis dan agama melalui metaanalisis atas pelbagai penelitian yang dituangkan dalam bentuk tesis dan disertasi. Penelitiannya sebagaian besar perempuan, baik warga negara indonesia maupun warga negara asing. Kelompok etnis yang diteliti cukup banyak dan terdapat di pelbagai pulau. hasilnya menunjukkan bahwa telah terjadi perubahan status dan peran perempuan dalam budaya dan agama yang berbeda ke arah yang menguntungkan perempuan dan laki-laki. Perubahan itu tampak khususnya dalam hubungan perempuan dengan agama, denan perkawinan dan dengan sektor usaha. Meskipun demikian, ketimpangan gender belum sepenuhnya hilang karena nilai-nilai budaya ataupun keagamaan yang hidup dalam pebagai masyarakat itu tidak seluruhnya mendorong perubahan. "

"This review article gives a brief history of the classical experiments that led to the development of the embryo culture medium and in vitro embryo culture. It proposes that, in view of the outstanding and significant pioneering contributions of Wesley Kingston Whitten to the development of embryo culture medium, he be considered the “Father of Embryo Culture Medium”. Furthermore, it describes the nutritional requirements of early embryos and how these requirements with specific references to carbohydrates, amino acids, phosphates, growth factors, etc, have been utilized to formulate increasingly more complex embryo culture media. This has led to the development of progressively more efficacious embryo culture media including the formulation of completely defined and synthetic protein-free embryo culture medium. The review also describes physical factors, growth factors, insemination methods for the fertilization of oocytes and culture methods affecting embryo growth, development, metabolism, oxygen embryotoxicity and survival. In procedural terms, the review also summarizes the evolution of embryo culture techniques from tube culture to, microdrop culture under oil to co-culture to ultra microdrop culture techniques. It includes techniques of in vitro maturation and for the selection of potentially viable embryos of various developmental stages"

"“Cancel culture” has become one of the most charged concepts in contemporary culture and politics, but mainstream critiques from both the left and the right provide only snapshots of responses to the phenomenon. Takinga media and cultural studies perspective, this book traces the origins of cancel practices and discourses, and discusses their subsequent evolution within celebrity and fan cultures, consumer culture, and national politics in the U.S. and China. Moving beyond popular press accounts about the latest targets of cancelling or familiar free speech debates, this analysis identifies multiple lineages for both cancelling and criticisms about cancelling, underscoring the various configurations of power associated with “cancel culture” in particular cultural and political contexts. "

"Latar Belakang: Asam hialuronat (AH) merupakan glikosaminoglikan dan salah satu komponen penting dari matriks ekstraseluler pada lingkungan biologis mikro dentin.  Pada pulpa terinflamasi, ketika lingkungan biologis mikro kondusif maka terjadi rekrutmen sel punca pulpa (human dental pulp stem cells/hDPSCs) yang akan berdiferensiasi menjadi odontoblast like cell membentuk dentin reparatif dan terdapat ekspresi dentin sialophosphoprotein (DSPP).Tujuan: Mengetahui potensi berbagai konsentrasi AH pada media kultur hDPSCs terhadap ekspresi DSPP dengan waktu observasi  7 hari dan 14 hari. Metode: hDPSCs yang didapatkan dari bahan baku tersimpan pada pasase ke-3 dan 4 yang telah mengalami serum starvation selama 24 jam, diberikan AH dengan konsentrasi 10 mg/mL , 20 mg/mL , 30 mg/mL dan  kontrol positif pada medium osteogenik. Selanjutnya dilakukan observasi waktu selama 7 hari dan 14 hari untuk melihat ekspresi DSPP dari tiap kelompok secara kuantitatif (uji ELISA) dan potensi mineralisasi secara kualitatif (uji alizarin red) pada hari ke-21. Uji statistik menggunakan one way anova. Hasil: Terdapat perbedaan potensi berbagai konsentrasi  AH (10mg/mL, 20  mg/mL, 30 mg/mL)  (p<0.05) pada media kultur hDPSCs terhadap ekspresi DSPP dengan waktu observasi 7 hari dengan konsentrasi 30 mg/mL yang paling berpotensi meningkatkan DSPP dan dikonfirmasi dengan nodul yang pekat pada uji alizarin red di hari ke-21. Kesimpulan: Asam hialuronat (AH) memiliki potensi untuk meningkatkan ekspresi DSPP, konsentrasi AH 30 mg/ml merupakan konsentrasi yang optimum bagi hDPSCs. 

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Background: Hyaluronic acid (HA) is glycosaminoglycan and one of important factors in extracellular matrix located at dentin niche biology.  In an inflamed pulp, when niche biology is conducive, the recruitment of human dental pulp stem cells (hDPSCs) will take place and it will differentiate into odontoblast like cell that will create reparative dentin and expressing dentin sialophosphoprotein (DSPP). 

Objective: To analyze the potential of HA conditioned media in various concentration towards hDPSCs differentiation via expression of DSPP at day 7 and 14. 

Methods: hDPSCs culture were obtained from previous research (ethical approval attached) at P3 and P4. After 24 hours incubation of hDPSCs, culture media were supplemented with osteogenic media. Cells were then starved for 24 hours. hDPSCs then planted into 96 well plate and HA 10 mg/ml, 20 mg/ml, 30 mg/ml added into each particular well. DSPP expression is analysed using elisa reader at day 7 and 14 and qualitative result is analysed using alizarin red at day 21. Data was analysed using one way anova.

Result: At day 7 there is a statistically significant different potential of natural HA conditioned media in various concentration (10mg/mL, 20  mg/mL, 30 mg/mL) (p<0.05) towards hDPSCs differentiation via expression of DSPP with HA 30 mg/mL being the most potential concentration to increase DSPP expression, which can be confirmed by bold nodules presented with alizarin red at day 21. 

Conclusion: HA have the potential to increase odontoblast differentiation process via expression of DSPP, with HA 30 mg/mL being the optimum concentration for hDPSCs."