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"[Tesis ini membahas tentang budaya produksi dalam newsroom TVONE pada pilpres 2014. Budaya produksi media, berkaitan dengan komposisi dan struktur sosial internal dari para pekerja media dan hubungan yang dipelihara. Penelitian ini merupakan penelitian kualitatif dengan menggunakan studi kasus di dalam program “Presiden Pilihan Rakyat”. Untuk mengetahui budaya produksi dalam newsroom TVONE pada pilpres 2014, peneliti menggunakan observasi dan wawancara mendalam terhadap jurnalis TVONE. Hasil penelitian menunjukanbahwa individual level, level rutinitas media, level organisasi, level ekstra media serta level ideologi berperan dalam budaya produksi pada newsroom TVONE di pilpres 2014.;This research focused on the cultural production in newsroom TVONE at the 2014 presidential election. Media production culture, with regard to the composition and internal social structure of the media workers and relationships are maintained. This is a qualitative research that using case study on Presiden Pilihan Rakyat programme. To find out cultural production in newsroom TVONEat the 2014 presidential election, therefore the author use case study on Presiden Pilihan Rakyat TV programme. In order to find out the cultural production in newsroom TVONE at the 2014 presidential election, the author observed and interview the TVONE journalist. The results showed that individual level, routines media level, organization level, extra media level and ideology play a role in the cultural level of production in the newsroom of TVONE at the 2014 presidential election, This research focused on the cultural production in newsroom TVONE at the2014 presidential election. Media production culture, with regard to thecomposition and internal social structure of the media workers and relationshipsare maintained. This is a qualitative research that using case study on PresidenPilihan Rakyat programme. To find out cultural production in newsroom TVONEat the 2014 presidential election, therefore the author use case study on PresidenPilihan Rakyat TV programme. In order to find out the cultural production innewsroom TVONE at the 2014 presidential election, the author observed andinterview the TVONE journalist. The results showed that individual level,routines media level, organization level, extra media level and ideology play arole in the cultural level of production in the newsroom of TVONE at the 2014presidential election]"

"Tujuan dari penelitian ini adalah untuk menilai terjadinya aktivasi endotel sebagai mekanisme palagenesis pada preeklampsia, dengan melihat pengaruh pajanan serum penderita preeklampsia pada kultur sel endotel vena umbilikalis terhadap produksi VCAM-1.RANCANGAN PENELITIAN: Penelitian ini merupakan studi eksperimental. Kultur sel endotel yang padat (confluent) dipajankan dengan medium yang mengandung 20% serum dad wanita preeklampsia (n = 12) atau wanita hamil normal (n = 12) dengan usia, usia kehamilan dan paritas yang tidak berbeda berrnakna secara statistik. Setelah pajanan selama 24 jam, diukur kadar VCAM-1 terlarut (sVCAM-1) dalam supematan kultur sel endotel dari ke-2 populasi tersebut. Jumlah sel endotel hidup dari kultur sel endotel pasca pajanan dihitung, baik pasca pajanan serum preeklampsia maupun wanita hamil normal. Diukur juga kadar sVCAM-1 dalam medium yang mengandung 20% serum dari wanita yang sama, yang tidak dipajankan dengan preeklampsia.HASIL: Kadar sVCAM-1 dalam supematan kultur setelah pajanan pada 10.000 set endotel selama 24 jam dengan serum preeklampsia (1.366 + 0.714 ng/ml) lebih tinggi secara bermakna (P < 0.05) dibandingkan setelah pajanan dengan serum wanita hamil normal (0.735 + 0.372 nglml). Jumlah sel endotel dari kultur sel endotel setelah pajanan dengan serum preeklampsia (9.00 x 104 + 3.77 x 104) lebih rendah dibandingkan setelah pajanan dengan serum wanita hamil normal (12.67 x 104 + 6.23); tetapi perbedaannya secara statistik tidak bermakna (P > 0.05). Kadar sVCAM-1 dalam medium kuttur yang mengandung 20% serum preeklampsia yang tidak dipajankan pada kultur sel endotel (11.0516 } 5.404 ng/lml) lebih tinggi dibandingkan serum wanita hamil normal (10.417 + 6.870 ng/ml); tetapi perbedaannya secara statistik tidak bermakna (P > 0,05)KESIMPULAN: Pajanan serum penderita preeklampsia pada kultur sel endotel vena umbilicalis menyebabkan terjadinya peningkatan produksi VCAM-1 oleh sel endotel, sehingga dapat disimpulkan bahwa pads preeklampsia terjadi aktivasi endotel akibat adanya suatu zat dalam serum penderita preeklampsia. Kata kunci: Preeklampsia, aktivasi endotel, kultur set endotel vena umbilikalis, Vascular Cell Adhesion Molecule-1.

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The Effect Of Preeclamptic Sera Exposure To Human Umbilical Vein Endothelial Cell Culture To The Production Of Vascular Cell Adhesion Molecul-1 (VCAM-1)OBJECTIVE: To determine endothelial activation as a pathogenic mechanism of preeclampsia, by identifying the effect of preeclamptic sera exposure to human umbilical vein endothelial cell culture on the production of VCAM-1.STUDY DESIGN: The study was an experimental study. Confluent endothelial cell culture exposed to medium with 20 % preeclamptic sera (n=12) on women with normal pregnancy sera (n=12) with the same age, gestational age and parity. After 24-hour of exposure, cultured media were removed for measurement of VCAM-1. The concentration of sVCAM-1 in medium with 20 % sera from the same women that was not exposed to cultured endothelial cell were also measured.RESULTS : The concentration of sVCAM-1 from 10.000 cultured endothelial cells media after 24-hour exposure with preeclamptic sera (1.366 + 0.714 nglml) was significantly higher than exposure with normal pregnant women sera (0.735 + 0.372). The amount of cultured endothelial cells after exposure to preeclamptic sera (9.00 x 104 + 3.77 x 104) was lower than after exposure to normal pregnant women sera (12.67 x 104 + 6.23); but the difference was not statistically significant (P a 0.05). Without exposure to cultured endothelial cells, the concentration of sVCAM-1 in the medium with 20 % preeclamptic sera was higher (11.0516 + 5.404 nglml) than in the medium with 20% sera from normal pregnant women (10.417 + 6.870 nglml), although the difference was not statistically significant (P > 0.05).CONCLUSIONS: Exposure of human umbilical vein endothelial cell culture to preeclamptic sera increased the production of VCAM-1 by the endothelial cells. It was concluded that there was endothelial activation in preeclampsia caused by factor or factors in preeclamptic sera."