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"The definitive endodontics reference, Cohen's Pathways of the Pulp is known for its comprehensive coverage of leading-edge information, materials, and techniques. It examines all aspects of endodontic care, from preparing the clinician and patient for endodontic treatment to the role the endodontist can play in the treatment of traumatic injuries and to the procedures used in the treatment of pediatric and older patients. Not only does Hargreaves and Cohen's 10th edition add five chapters on hot new topics, it also includes online access! As an Expert Consult title, Cohen's Pathways of the Pulp lets you search the entire contents of the book on your computer, and includes five online chapters not available in the printed text, plus videos, a searchable image collection, and more. For evidence-based endodontics research and treatment, this is your one-stop resource!"

"Latar belakang: xylitol merupakan gula alkohol (polyols) dengan 5 ikatan rantai karbon yang dilaporkan bermanfaat bagi kesehatan manusia. Dalam bidang kedokteran gigi, xylitol memiliki peran sebagai bahan antikaries gigi karena dapat menghambat pertumbuhan bakteri Streptococcus mutans. Namun, efek xylitol terhadap sel-sel pulpa gigi belum diketahui. Pulpa gigi merupakan jaringan yang sensitif terhadap paparan benda asing. Pada pulpa gigi yang terbuka, xylitol dapatmenimbulkan efek biologik sel. Tujuan: untuk mendeteksi efek paparan xylitol terhadap protein total dan profil protein medium kultur sel-sel pulpa gigi. Metode: sel-sel pulpa gigi didapat dari jaringan pulpa gigi sehat yang baru diekstraksi, kemudian dikultur dalam medium DMEM (37ºC, 5% CO2) hingga confluent. Kemudian dilakukan subkultur dengan kondisi yang sama selama semalam. Kelompok perlakuan dipaparkan xylitol dengan konsentrasi 2%, 4%, 8%, dan 16%, tetapi kelompok kontrol tidak dipapar xylitol. Konsentrasi protein total medium kultur sel-sel pulpa gigi diukur dengan menggunakan Bradford protein assay pada panjang gelombang 655 nm. Sedangkan profil protein medium kultur sel-sel pulpa gigi dianalisis dengan teknik SDS PAGE. Hasil: rerata konsentrasi protein total (µg/ml ± SD) medium kultur sel-sel pulpa gigi pada kelompok perlakuan xylitol 2% (24.253,71 ± 2.363,29), xylitol 4% (21.925,42 ± 1.001,38), xylitol 8% (25.456,51 ± 4.569,45), dan xylitol 16% (26.306,66 ± 5.550,82) secara statistik dengan Oneway ANOVA lebih rendah bermakna (p<0,05) dibandingkan dengan kontrol (33.395,64 ± 4.209,08). Dari hasil SDS PAGE, ternyata tidak terjadi perubahan profil protein medium kultur sel-sel pulpa gigi setelah pemaparan xylitol. Simpulan: konsentrasi protein total medium kultur sel-sel pulpa gigi menurun setelah pemaparan dengan xylitol, namun profil protein medium kultur sel-sel pulpa gigi tidak mengalami perubahan.

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Background: xylitol is one of sugar alcohol (polyols) with 5 carbon atoms which is reported to have benefits to our health. In dentistry, xylitol has anti-caries effect as the growth of Streptococcus mutans could be inhibited. However, the xylitol effects on dental pulp have not been known yet. Dental pulp tissue is sensitive to foreign substances. Xylitol could penetrate the exposed dental pulp and induce the biological response of the cells.

Objective: to detect the effects of xylitol on dental pulp cells determined by total protein and protein profile of culture medium of the dental pulp cells (in vitro).

Methods: dental pulp cells were obtained from healthy and freshly extracted teeth. Then, they were cultured in DMEM medium (37ºC, 5% CO2) until confluent approximately 2 days. Subsequently they were subcultured and used as samples. The treatment groups were treated with xylitol 2%, 4%, 8%, and 16% and incubated at 37°C, 5% CO2 for overnight, while the control groups without xylitol. The total protein of culture medium was determined by Bradford protein assay in 655 nm. Whereas, the protein profile of culture medium were analized by SDS PAGE method.

Results: the mean of total protein? concentration (µg/ml ± SD) of culture medium in treatment groups with xylitol 2% (24.253,71 ± 2.363,29), xylitol 4% (21.925,42 ± 1.001,38), xylitol 8% (25.456,51 ± 4.569,45), dan xylitol 16% (26.306,66 ± 5.550,82) were lower than control group (33.395,64 ± 4.209,08). The comparison between the controls and treatment groups were analysed by Oneway ANOVA. All the treatment groups were signifcantly different compared with the controls (p<0,05). By SDS PAGE, the protein profile of culture medium in all treatment groups was not altered.

Conclusion: the total protein? concentration of culture medium of the dental pulp cells were decreased after treated with xylitol. However, the protein profile of culture medium of dental pulp cells was not altered."

"Tujuan dari penelitian ini adalah untuk menginvestigasi efektivitas dental pulp stem cells DPSCs dalam menginduksi proses regenerasi jaringan pada defek tulang kelinci New Zealand dengan menilai kadar alkaline phosphatase ALP dan gambaran histologis. Defek kritis dibuat pada tulang femur kelinci dan transplantasi DPSCs dilakukan terhadap kelompok perlakuan, sedangkan defek pada kelompok kontrol dibiarkan kosong. Pada minggu ke-2 dan ke-4 pasca tindakan operatif, dilakukan pengukuran kadar ALP dalam serum menggunakan colorimetric assay. Setelah 4 minggu, kelinci dikorbankan dan dilakukan analisis terhadap gambaran histologis. Hasil penelitian menunjukkan bahwa pada minggu ke-2, kelompok kelinci yang diberi perawatan dengan DPSCs memiliki kadar ALP yang lebih tinggi 157,925 ?U daripada kelompok kontrol 155,361 ?U dan peningkatan terjadi di minggu ke-4 dengan nilai yang lebih besar pada kelompok DPSCs 169.750 ?U dibandingkan dengan kelompok kontrol 160.406 . Evaluasi histologis menunjukkan bahwa sejumlah lamela tulang dan osteosit mengisi area defek dari kelompok DPSCs. Dengan demikian, dapat disimpulkan bahwa transplantasi DPSCs efektif dalam menginduksi dan mempercepat progresivitas regenerasi jaringan.

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This study was aimed to investigate the effectiveness of dental pulp stem cells DPSCs to induce bone regeneration in New Zealand rabbits by assessing the level of alkaline phosphatase ALP and histological view. The critical defect was created in the left femoral bone of the rabbits and transplantation of DPSCs was conducted to the treated group while the defect in the control group was left empty. In 2nd week and 4th week postoperative, ALP level in rabbits serum were measured using colorimetric assay. After 4 weeks, the rabbits were sacrificed and analyzing of histological views were conducted.

The results showed that in the 2nd week, rabbit treated DPSCs group had higher level of ALP 157,925 U than the control group 155,361 U and increasing occured in the 4th week with greater score in DPSCs group 169.750 U compared to the control group 160.406 U . Histological evaluation revealed that the amount of bone lamellae and osteocytes filled the defect area of DPSCs group. Therefore, transplantation of DPSCs are effective to induce and accelerate bone regeneration by raising ALP level and forming new bone tissue."

"Latar belakang: xylitol adalah gula alkohol berantai karbon lima (polyol) yang banyak digunakan sebagai pemanis alami dalam bentuk permen karet untuk mencegah karies gigi. Xylitol memiliki efek antikaries karena dapat menghambat pertumbuhan S. mutans yang merupakan salah satu agen utama penyebab karies gigi, menurunkan pembentukan plak dan meningkatkan remineralisasi gigi. Pulpa gigi berperan penting bagi vitalitas gigi. Pada pulpa gigi yang terbuka, xylitol dapat berpenetrasi dan menimbulkan efek biologik pada sel. Tujuan: untuk mendeteksi efek xylitol terhadap viabilitas dan profil protein sel-sel pulpa gigi (in vitro). Metode: sel-sel pulpa gigi didapat dari gigi sehat yang baru diekstraksi, dan dikultur dalam medium kultur DMEM (37°C, 5% CO2) hingga confluent. Selanjutnya sel-sel tersebut disubkultur pada kondisi yang sama selama semalam di 24-wellplate. Setelah itu kelompok perlakuan dipaparkan xylitol dengan konsentrasi 2%, 4%, 8% dan 16%. Sedangkan pada kelompok kontrol tidak diberi xylitol. Viabilitas sel diukur dengan MTT assay. Sedangkan profil protein dianalisis dengan SDS PAGE. Hasil: rerata optical density (OD) kelompok xylitol 2% (1,784 ± 0,052), 4% (2,465 ± 0,057), 8% (2,168 ± 0,162), dan 16% (1,912 ± 0,148) lebih tinggi dibandingkan dengan kelompok kontrol (1,566 ± 0,069). Uji statistik Oneway ANOVA menunjukkan bahwa seluruh kelompok perlakuan berbeda bermakna dengan kontrol (p<0,05). Persentase viabilitas sel diperoleh dari rerata optical density. Viabilitas sel kelompok xylitol 2% (113,92%), 4% (157,40%), 8% (138,44%), dan 16% (122,09%) lebih tinggi dibandingkan dengan kelompok kontrol (100%). Dari hasil SDS PAGE, tampak perubahan profil protein sel-sel pulpa gigi. Simpulan: terdapat peningkatan viabilitas sel dan perubahan profil protein sel-sel pulpa gigi setelah pemaparan xylitol. ......Background: xylitol is five carbon sugar alcohol (polyol) which is used as natural sweetener in chewing gum to prevent dental caries. Xylitol has anticaries effect as it can inhibit the growth of S. Mutans, one of the main etiology of dental caries, decrease plaque formation, and increase tooth remineralization. Dental pulp has an important role in dental vitality. In exposed dental pulp, xylitol can penetrate and induce biological response of the cells. Objective: to detect the effects of xylitol to cell viability and protein profile of dental pulp cells (in vitro). Method: dental pulp cells were obtained from healthy and freshly extracted teeth, and were cultured in DMEM (37°C, 5% CO2) until confluent. Subsequently, they were subcultured in same condition overnight on 24-well plate. Afterwards, the treatment groups were exposed by 2%, 4%, 8%, and 16% xylitol. Whilst, the control group was not exposed by xylitol. Cell viability was measured by MTT assay. Whereas, the protein profile was analized by SDS PAGE. Results: the mean of optical density of treatment group with xylitol 2% (1,784 ± 0,052), 4% (2,465 ± 0,057), 8% (2,168 ± 0,162), and 16% (1,912 ± 0,148) were higher than control group (1,566 ± 0,069). Statistical test Oneway ANOVA showed that all the treatment groups were significantly different compared with the control (p<0,05). The percentage of cell viability was obtained from the mean of optical density. The cell viability of xylitol 2% (113,92%), 4% (157,40%), 8% (138,44%), dan 16% (122,09%) were higher than control group (100%). From SDS PAGE, there was protein profile alteration. Conclusion: there was an increased of cell viability and the alteration of protein profile of dental pulp cells after treated with xylitol."

"Latar belakang: Trietylene glycol dimethacrylate (TEGDMA) merupakan salah satu monomer yang terkandung dalam resin komposit. Jika polimerisasi resin komposit tidak sempurna, TEGDMA dapat terlepas ke dalam rongga mulut dalam beberapa menit hingga jam dan dapat berpenetrasi mencapai pulpa. TEGDMA dilaporkan bersifat toksik terhadap sel dan jaringan rongga mulut. Tujuan: Mengetahui efek TEGDMA terhadap sel-sel pulpa gigi ditentukan berdasarkan viabilitas dan profil protein sel pulpa (in vitro). Metode: Sel-sel pulpa berasal dari jaringan pulpa gigi sehat yang baru diekstraksi, kemudian dikultur dalam DMEM (37o C, 5% CO2) sampai confluent (± 2 malam). Selanjutnya dilakukan subkultur dengan kondisi yang sama selama 1 malam pada 24-wellplate. Kemudian pada kelompok perlakuan dipaparkan TEGDMA dengan konsentrasi 4 mM, 8 mM dan 12 mM selama 24 jam; sedangkan pada kelompok kontrol tidak dipaparkan TEGDMA. Viabilitas sel diukur dengan menggunakan MTT assay dan hasilnya dibaca dengan microplate reader (490 nm), sedangkan gambaran profil protein dideteksi dengan menggunakan SDS-PAGE dan diinterpretasikan dengan menggunakan Gel Doc. Hasil: Rerata optical density (OD) ± SD kelompok perlakuan TEGDMA 4 mM (1,71 ± 0,08); 8 mM (1,59 ± 0,11); dan 12 mM (1,50 ± 0,16) lebih rendah dibandingkan dengan kelompok kontrol (1,81 ± 0,11). Uji statistik One Way ANOVA menunjukkan bahwa nilai rerata OD kelompok TEGDMA 8 mM dan 12 mM berbeda bermakna dengan kelompok kontrol (p<0.05). Profil protein sel mengalami perubahan setelah pemaparan TEGDMA. Kesimpulan: Pada penelitian ini viabilitas sel menurun dan terjadi perubahan profil protein sel setelah pemaparan TEGDMA.

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Background: Trietylene glycol dimethacrylate (TEGDMA) is one of monomer contained in composite resin. If the polimerized was incomplete TEGDMA could bereleased into oral cavity in minutes to hours and could penetrate to the dental pulp. Itwas reported that TEGDMA has cytotoxic effects to cells and tissues in oral cavity.

Objectives: To determine the toxic effect of TEGDMA on dental pulp cells culture based on cell viability and Protein Cell Profile.

Methods: The pulp cells were isolated from the pulp tissue of the freshly extracted teeth, cultured in DMEM (37o C, 5% CO2) until confluent (± 2 nights). Afterwards, subcultured with the same condition overnight in 24-wellplate. Then, the treatment groups were treated with TEGDMA 4 mM, 8 mM, dan 12 mM for 24 hours, whereas in control group without TEGDMA exposure. The optical density of cell viability was measured by MTT assay then it was read with microplate reader in 490 nm. The protein cell profile was identified by SDS-PAGE method and analyzed by Gel Doc.

Results: Mean optical density ± SD of TEGDMA treatment group 4mM (1,71 ± 0,08), 8mM (1,59 ± 0,11), and 12 mM (1,50 ± 0,16) were lower than the control group (1,81 ± 0,11). One Way ANOVA analysis showed that TEGDMA treatment group 8 mM and 12 mM had significant differences compared with the control group (p<0,05). The protein profile of cells was altered after TEGDMA exposure.

Conclusion: In this research the cell viability was decreased and the protein profile of cells was altered after TEGDMA exposure."

"Latar belakang: xylitol adalah gula alkohol dengan 5 ikatan rantai karbon yang memiliki banyak manfaat bagi kesehatan manusia. Dalam bidang kedokteran gigi, xylitol memiliki peran sebagai antikaries gigi karena dapat menghambat pertumbuhan bakteri Streptococcus mutans penyebab karies gigi. Namun belum diketahui efek pemaparan xylitol terhadap sel-sel pulpa gigi. Pulpa gigi merupakan jaringan yang sensitif terhadap paparan benda asing. Pada pulpa gigi yang terbuka, xylitol dapat menimbulkan efek biologik. Tujuan: untuk mendeteksi efek paparan xylitol dalam beberapa konsentrasi terhadap protein total dan profil protein sel-sel pulpa gigi secara in vitro. Metode: sampel penelitian berasal dari sel-sel pulpa gigi sehat (tanpa karies) yang baru diekstraksi. Selanjutnya dikultur selama semalam dan dilanjutkan dengan subkultur selama semalam. Kemudian kelompok perlakuan xylitol dipaparkan xylitol dengan konsentrasi 2%, 4%, 8%, dan 16%, sedangkan kelompok kontrol tidak diberi paparan xylitol. Protein total sel-sel pulpa gigi diukur dengan menggunakan metode Bradford assay dan profil protein sel-sel pulpa gigi dianalisis dengan menggunakan metode SDS PAGE. Hasil: rerata konsentrasi protein total (µg/ml ± SD) sel-sel pulpa gigi kelompok perlakuan xylitol 2% (23031,305 ± 1636,87), kelompok perlakuan xylitol 4% (26380,865 ± 3278,0), kelompok perlakuan xylitol 8% (23192,574 ± 1441,39), dan kelompok perlakuan xylitol 16% (21498,481 ± 2633,37) memiliki rerata konsentrasi protein total sel-sel pulpa gigi yang lebih tinggi dibandingkan kelompok kontrol (19013,045 ± 2188,51) dan memiliki perbedaan bermakna berdasarkan uji statistik Oneway ANOVA. Namun, antar kelompok perlakuan xylitol 2%, 4%, 8% dan 16% tidak terdapat perbedaan bermakna (p<0,05). Pada gambaran profil protein, tampak terjadi perubahan profil protein pada kelompok perlakuan xylitol 2% dan 8%. Simpulan: pada penelitian ini terjadi peningkatan konsentrasi protein total dan perubahan profil protein selsel pulpa gigi setelah pemaparan xylitol.

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Background: xylitol is sugar alcohol with 5 carbon atom in the molecule which has many benefits for human health. In dentistry, xylitol is an anti-cariogenic agent as it can inhibit Streptococcus mutans growth. Nevertheless, the effect of xylitol exposure to dental pulp cells has not been known yet. Dental pulp is a sensitive tissue toward exposure of several agents. In the exposed dental pulp, xylitol can cause biological effects.

Objectives: the effect of xylitol with several concentrations determined to total protein and protein profile of the dental pulp cells culture.

Methods: the dental pulp cells were obtained from healthy and freshly extracted teeth (non-caries). Furthermore, dental pulp cells were cultured overnight and then subcultured another overnight. Afterwards, xylitol treatment group was exposured by 2%, 4%, 8%, and 16% xylitol, while control group was not exposured by xylitol. Total protein cells was measured by Bradford assay method and protein profile was analized by SDS PAGE.

Results: the mean of total protein (µg/ml ± SD) cells concentration? of 2% xylitol group (23031,305 ± 1636,87), 4% xylitol group (26380,865 ± 3278,0), 8% xylitol group (23192,574 ± 1441,39), and 16% xylitol group (21498,481 ± 2633,37) were statistically higher than the control group (19013,045 ± 2188,51). However, there were not significant differences between 2%, 8%, and 16% xylitol groups. From the result of SDS PAGE, it was shown that there was altered protein profile in 2% and 8% xylitol group.

Conclusions: in this research, the concentration of total protein cells were increased and the cells protein profile was altered in the dental pulp cells after xylitol exposured."

"Latar belakang: Trietylene glycol dimethacrylate (TEGDMA) merupakan salah satu monomer yang terkandung dalam bahan tambal resin komposit. Jika polimerisasi tidak sempurna, TEGDMA dapat dengan mudah terlepas ke dalam rongga mulut beberapa menit hingga jam setelah penambalan, dan dapat berpenetrasi ke dalam pulpa. TEGDMA yang terlepas dilaporkan bersifat toksik terhadap jaringan dan sel tubuh.Tujuan: menentukan efek TEGDMA terhadap protein total dan profil protein sel-sel pulpa gigi.Metode: sel-sel pulpa didapat dari jaringan pulpa gigi sehat yang baru diekstraksi, kemudian dikultur pada medium kultur DMEM. Setelah sel kultur tampak confluent (±2 malam), dilakukan subkultur dari kultur primer tersebut, yang kemudian diinkubasi kembali pada suhu 37° C dan 5% CO2 selama 1 malam pada 24- well plate. Kemudian dilakukan pemaparan TEGDMA dengan konsentrasi 4mM, 8mM dan 12mM, selama 24 jam, sedangkan pada kelompok kontrol tidak dilakukan pemaparan TEGDMA. Penentuan konsentrasi protein total sel pulpa dilakukan dengan menggunakan Bradford protein assay. Profil protein sel pulpa diidentifikasi dengan teknik SDS-PAGE. Analisa berat molekul protein sampel dilakukan dengan menggunakan Gel Doc (Band Analysis Quick Guide).Hasil: Rerata konsentrasi protein total sel (µg/ml ± SD) pada kelompok perlakuan dengan TEGDMA 4mM (22762,27±3385,87) dan 8mM (20268,44±1701,14) memiliki nilai dibawah kelompok kontrol (24253,77±3072,88). Sedangkan kelompok perlakuan dengan TEGDMA 12mM (23706,51±3214,52) memiliki nilai konsentrasi protein total sel di atas kelompok 4mM dan 8mM, namun masih tetap di bawah kelompok kontrol. Berdasarkan uji statistik dengan one way ANOVA, hanya kelompok TEGDMA 8mM yang memiliki perbedaan rerata konsentrasi protein total sel yang bermakna (p=0.037) terhadap kelompok kontrol. Selanjutnya dari gambaran profil protein yang terbentuk pada gel elektroforesis, tampak perubahan profil protein sel pada setiap kelompok setelah paparan TEGDMA.Kesimpulan: Pada penelitian ini terjadi penurunan konsentrasi protein total sel dan perubahan profil protein sel pulpa gigi setelah pemaparan TEGDMA dengan konsentrasi 4mM, 8mM, dan 12mM.

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Background: Trietylene glycol dimethacrylate (TEGDMA) is one of monomer that contained in composite resin restoration. When polymerization is incomplete, this compound could leache into oral cavity in minutes to hours after the restoration, and could penetrate the dental pulp. TEGDMA has been reported to be cytotoxic to the tissues and cells.Objective: to determine the effects of TEGDMA on total protein and protein profile of the dental pulp cells.Methods: the dental pulp cells were collected from the dental pulp tissues of the freshly extracted teeth, and cultured in culture medium of DMEM. After the growth of cultured cells was confluent (±2 nights), the cells were subcultured then incubated (37°C, 5% CO2) overnight in 24-well plate. Afterwards, the cells were exposed to TEGDMA with concentrations of 4mM, 8mM, and 12mM, for 24 hours, meanwhile in control group without TEGDMA exposure. The concentration of total cell protein was measured by Bradford protein assay. The protein profile of the dental pulp cells were identified by SDS-PAGE. The molecular weight of sample protein was analyzed by Gel Doc (Band Analysis Quick Guide).Results: The mean of total cell protein concentration (µg/ml ± SD) on treatment groups of 4mM TEGDMA (22762,27±3385,87) and 8mM (20268,44±1701,14) were lower than the controls (24253,77±3072,88). Whereas, the total cell protein concentration of treatment group of TEGDMA 12mM (23706,51±3214,52) was higher than the treatment groups with TEGDMA 4mM and 8mM, but it was still lower than the controls. According to one way ANOVA statistic test, only the treatment group with TEGDMA 8mM was significantly lower than the controls (p=0.037). Furthermore, the protein profile identified by electrophoresis gel, showed the profile alteration after TEGDMA exposure.Conclusion: In this research the total cell protein concentration was decreased and the protein profile of the dental pulp cells was altered after exposure with TEGDMA 4mM, 8mM, and 12mM."

"Latar Belakang: Ekstrak jintan putih Cuminum cyminum memiliki potensi efektivitas antibakteri dan anti jamur serta tidak toksik terhadap sel fibroblas tikus. Belum terdapat penelitian yang meneliti toksisitas ekstrak jintan putih terhadap Dental Pulp Stem Cells DPSCs . Tujuan: Mengetahui efek ekstrak jintan putih konsentrasi 0,1 mg/ml, 0,4 mg/ml, 0,7 mg/ml, dan 1,0 mg/ml terhadap viabilitas DPSCs. Metode: Menggunakan uji MTT dengan menghitung nilai absorbansi menggunakan microplate reader, dengan hasil akhir berupa nilai optical density OD yang dipersentasekan terhadap kelompok kontrol. Hasil: Terdapat perbedaan viabilitas DPSCs yang bermakna ......Introduction The extract of cumin Cuminum cyminum has the potential antibacterial and antifungal activity and it was not toxic for mouse fibroblasts. However, there have been no research investigating the toxicity of cumin extract on Dental Pulp stem Cells DPSCs . Aims To compare viability DPSCs of Cuminum cyminum extract 0,1 mg ml, 0,4 mg ml, 0,7 mg ml, and 1.0 mg ml . Methods Cell viability was analyzed using MTT Assay by calculating absorbance value using microplate reader, with optical density OD as the final result. Results There were significant differences statistically in viability on DPSCs p"

"Objective: To compare the importance of storage time and the tooth type for isolation of dental pulp cells (DPCs) from extracted human teeth.Methods: 35 human teeth were used in this study. The teeth were stored in phosphate buffered saline (PBS) after extraction and divided into two groups randomly according to the time elapsed between extraction and isolation. In group one, the isolation was performed within 2 hours and in the other group it was performed 24 hours after extraction.Results: No significant differences between isolation time and total cell counts (p 0.483) and between isolation time and viable cells (p 0.341). No significant differences between the first molar and the premolar related cell counts and viable cells, but both teeth groups showed significant higher viability and had higher total cell amounts than third molars after isolation. Statistically significant correlations were found between age of donors and viable cells and viability after 24 hours isolation time.Conclusion: The immediate isolation of DPCs is not necessary after the tooth extraction. The tooth can be stored in PBS at room temperature up to twenty four hours after the extraction without a significant reduction in cell viability and counts. The cells obtained from younger donors might have more chance for more viability even if storage time was extended. Premolars and first molars were better donors than the third molars for DPCs isolations and the high number of success revascularization rate in premolars with necrotic immature premolars might be because of their high cell viability potentials."