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Abstrak :
Metarhizium majus UICC 295 adalah kapang entomopatogen. Penelitian bertujuan membuat dan menguji formula M. majus UICC 295 dengan substrat beras (Oryza sativa) terhadap larva Oryctes rhinoceros, serta mengetahui viabilitas konidia/hifa di dalam formula selama penyimpanan 30 hari pada suhu ruang dan 4° C. Formula dibuat dengan menginokulasikan M. majus UICC 295 10% (berat/berat) pada beras. Aplikasi kontak langsung M. majus UICC 295 dengan jumlah konidia/hifa (0,69--1,63)x106 CFU/ml menyebabkan kematian larva 100% dalam 9--13 hari. Pengujian formula dengan jumlah konidia/hifa (0,82--1,7)x106 CFU/ml menyebabkan kematian larva 100% dalam 7--11 hari. Penyimpanan formula selama 30 hari pada suhu 27° C dan 4° C menyebabkan penurunan persentase viabilitas konidia berturut-turut sebesar 93,85% dan 90,95%. ......Metarhizium majus UICC 295 is an entomopathogenic fungus. This research investigated the use of rice (Oryza sativa) for formulation of M. majus UICC 295, formula application on Oryctes rhinoceros larvae, the effect of temperature and time of storage on viability of conidia/hyphae in the formula. Formulation was carried out by inoculation of 10% (w/w) fungal biomass into rice. Application of direct contact of conidia/hyphal suspension (0.69--1.63)x106 cfu/ml caused 100 % larval mortality in 9--13 days. Application of the formula containing conidia/hyphal suspension (0.82--1.7)x106 cfu/ml caused 100% larval mortality in 7--11 days. The conidia/hyphae viability in the formula was decreased 93.85% and 90.95% after storage for 30 days at 27° C and 4° C, respectively.

Abstrak :
Metarhizium majus UICC 295 adalah kapang entomopatogen. Penelitian bertujuan menguji kemampuan M. majus UICC 295 pada medium Sabouraud Dextrose with Yeast Extract Agar (SDYA) dengan penambahan tepung kulit udang 10% (b/v) dalam menginfeksi larva Oryctes rhinoceros serta mengetahui pengaruh preservasi metode freezing pada suhu -80o C menggunakan protektan gliserol 10% (v/v) dan gliserol 10% (v/v) dengan penambahan sukrosa 5% (b/v). Metarhizium majus UICC 295 pada SDYA mampu membunuh larva 3,33%--100% dalam 7--11 hari dan dengan penambahan tepung kulit udang 10% membunuh larva 6,67%--40% dalam waktu 12--30 hari. Metarhizium majus UICC 295 pada medium SDYA tetap memiliki viabilitas setelah dipreservasi pada suhu -80o C menggunakan gliserol 10% dan gliserol 10% dengan penambahan sukrosa 5%. Metarhizium majus UICC 295 pada SDYA dengan penambahan kulit udang 10% kehilangan viabilitasnya setelah dipreservasi pada suhu -80o C. Metarhizium majus UICC 295 pada kadaver larva O. rhinoceros tetap memiliki viabilitas setelah dipreservasi pada suhu -80o C. ......Metarhizium majus UICC 295 is an entomopathogenic fungus. This research investigated the pathogenicity of M. majus UICC 295 from Sabouraud Dextrose Agar with Yeast Extract (SDAY) medium added with 10% (w/v) shrimp shell powder to infect Oryctes rhinorecos larvae, and to determine the effect of preservation with freezing method at -80o C with 10% (v/v) glycerol and 10% (v/v) glycerol with addition of 5% (w/v) sucrose as protectants. Application of M. majus UICC 295 from SDYA caused 3.33%--100% larval mortality within 7--11 days, whereas addition of 10% shrimp shell powder caused 6.67%--40% larval mortality within 12--30 days. Metarhizium majus UICC 295 from SDYA was viable after being preserved at -80o C with 10% glycerol and 10% glycerol with addition of 5% sucrose as cryoprotectant, M. majus UICC 295 from SDYA with addition of 10% shrimp shell powder lost its viability after being preserved at -80o C with both cryoprotectants. Metarhizium majus UICC 295 on O. rhinoceros cadaver was viable after being preserved at -80o C.