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"Tujuan : Untuk mengetahui gambaran status vitamin B-12, asam folat, nutrisi dan profit lipid serum pada lanjut usia, agar dapat dimanfaatkan untuk pertimbangan pencegahan dan terapi penyakit jantung ; coroner (PJK) dan aterosklerosis.Tempat : Sepuluh puskesmas kecamatan di Jakarta Selatan,Cara : Studi cross-sectional pada lanjut usia z 60 tahun, subjek dipilih secara acak pada tingkat puskesmas. Data yang dikumpulkan meliputi sosio-demografi; pola makan; asupan energi, karbohidrat, lemak, protein, kolesterol, vitamin B-12, asam folat; kadar vitamin B-12; asam folat dan lipid serum, indeks massa tubuh (A.fl) dan rasio LPe-LPa.Hasil : Prevalensi kekurangan vitamin B-12 serum 34,6% dan kekurangan asam folat serum 34,0%. Konsentrasi vitamin B-12 serum dan asam folat serum pada pria lebih rendah dari wanita. Pada pria dan wanita vitamin B-12 serum kelompok umur z 70 tahun lebih rendah dibanding kelompok umur 60-69 tahun. Prevalensi hiperkolesterolemia dan kolesterol LDL serum yang tinggi (z 160 mg/dL) adalah, 42,6% dan 24,1%. Pada pria dan wanita kolesterol total serum pada kelompok umur z 70 tahun lebih rendah dibanding kelompok umur 60-69 tahun. Di lain pihak kolesteroI HDL serum pada pria dan wanita kelompok umur Z 70 tahun lebih tinggi dibanding kelompok umur 60-69 tahun. Rata-rata IMl' untuk pria 23,9 dan wanita 24,1 dan rata-rata rasio LPe-LPa untuk pria 0,93 dan wanita 0,85. Pada lanjut usia dengan konsentrasi vitamin B-I2 serum < 350 pg/mL berkorelasi positif dengan kolesterol HDL serum (r = 0,29; P = 0,03), tetapi tidak berkorelasi dengan kolesterol total serum, kolesterol LDL serum, rasio kolesterol total/ kolesterol HDL dan rasio kolesterol LDLlkolesterol HDL. Di lain pihak lanjut usia dengan konsentrasi vitamin B-I2 serum Z 350 mg/mL tidak berkorelasi dengan lipid serum.Kesimpulan : Melalui pendekalan faktor resiko PiK, prevalensi kekurangan vitamin B-12 dan kekurangan asam folat di Indonesia relatif tinggi dan sesuai dengan penelitan-penelitian yang telah dilakukan di negara-negara maju. Interaksi antara vitamin B-12 serum dan lipid serum belum dapat ditentukan sebagai interaksi yang linier tanpa adanya informasi mengenai homosistein serum. Kecukupan vitamin B-12 serum untuk lanjut usia sangatlah esensial untuk memperkecil terjadinya dislipidemia sebagai salah satu faktor resiko PJK.

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Objective : To determine vitamin B-l2, folic acid, anthropometric and serum lipid profiles of the Indonesian elderly which are considered to be important in the prevention and treatment of coronary atherosclerosis.

Place : Ten PHC in the district of South Jakarta.

Methods : A cross-sectional study on the elderly (z 60 year) was carried out in 10 PHCs, Subjects were drawn randomly at the PHC levels. Data collected were sosio-demography; food habits; intakes of energy, carbohydrate, fat, protein, cholesterol, vitamin B-12, and folic acid; serum vitamin B-12, serum folic acid, serum lipids; anthropometry [body mass index (BMI) and waist-hip ratio].

Results : The prevalence of biochemical vitamin B-12 and folic acid deficiencies were 34.6% and 34.0% respectively. Serum vitamin B-12 and folic acid concentrations of the elderly men were lower than those of the elderly women. Serum vitamin B-12 of both elderly men and women aged Z 70 years was lower than their younger counterparts aged 60-69 years. The prevalence of hypercholesterolemia and high serum LDL cholesterol (z 160 mg/di.) was 42.6% and 24.1% respectively. Mean serum total cholesterol of both elderly men and women aged z 70 years was lower than those aged 60-69 years old. On the other hand, serum HOL cholesterol of both elderly men and women aged z 70 years was higher than their younger counterparts aged 60-69 years. Mean BMI values were 23.9 kglm2 for the elderly men and 24,1 kglrn2 for the elderly women. Mean waist-hip ratios for the elderly men and women were 0.93 and 0.85 respectively, In the elderly subjects with low serum vitamin B-12 (< 350 pg/mL), positive correlations were found between serum vitamin B-12 and serum HDL cholesterol (r 0.29; P = 0.03), but not with any of serum total cholesterol, serum LDL cholesterol, total cholesterol/HDL cholesterol ratio, and LDLIHDL ratio. On the other hand, in the elderly subjects with normal and high serum vitamin B-12 (z 350 pglmL), there were no correlations between serum vitamin B-I2 and serum lipids.

Conclusions: Using the CHD-risk approach, the prevalence of biochemical vitamin B-12 and folic acid deficiencies of the Indonesian elderly was relatively high and comparable with existing studies in developed countries. Without information on serum homocysteine concentration, the interactions between serum vitamin B-12 and lipids were not linear. Clearly, adequacy of serum vitamin B-12 for the elderly is essential to minimize disorder of lipid metabolism as one amongst other CHD risk factors."

"Tujuan: Mengetahui status asam folat dan perilaku tentang asupan asam folat pada akseptor KB, sehingga dapat dipertimbangkan perlu tidaknya suplementasi asam folat pada akseptor KB, khususnya akseptor pil. Tempat: RW 014 Kelurahan Pisangan Baru, Kecamatan Matraman, Jakarta Timur. Bahan dan Cara: Penelitian crows-sectional dan subjek penelilian adalah semua akseptor KB yang memenuhi kriteria penelitian. Data yang dikumpulkan meliputi data sosio demografi, pola makan, asupan giri (makro matrial dan asam folat), status kadar asam folat serum dan sel darah merah (SDM). Hasil: Tidak didapatkan kadar asam folat serum < 3 ag/ml. Status asam folat serum subjek tidak berhubungan dengan pemakaian pil, karakteristik demografi, asupan gizi dan status gizi. Persentase kadar asam folat SDM < 160 mg/ml. adalah 3,5%. Kadar asam folat SDM subjek pil tidak berbeda dengan subjek bukan pil, namun ada kecenderungan kadar asam folat SDM subjek pil lebih rendah dari subjek bukan pil. Secara bermakna kadar asam folat SDM subjck bcrhubungan dengan kebiasaan mengkonsumsi sayur hijau dan kelompok umur. Perbedaan dalam mengolah sayur menyebabkan perbedaan bermakna pada jumlah subjek yang mempunyai kadar asam folat SDM < 160 mg/ml. Rata-rata asupan zat-zat gizi subjck penelitian di bawah AKG yang dianjurkan. Subjek pil mempunyai rata-rasa asupan asam folat lebih rendah bermakna dari subjek bukan pil, namun tidak didapatkan korelasi antara asupan asam folat dengan kadar asam folat serum dan SDM subjek penelitian. Kcsimpulan: Status asam folat serum dan SDM subjek penelitian tidak berhubungan dengan pemakaian pil dan bukan pil, sehingga belum diperlukan suplementasi pada subjek penelitian khususnya subjek pil.

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Objective: To determine the folic acid slates and the behavior of folic acid intake in contraceptive users. This is in order to decide whether folic acid supplemeration is necessary, especially for pill users.

Location: RW 014 Kelurahan Pisangan Baru, Kecamatan Matraman, Jakarta Timur.

Methods: cross-sectional study and subjects were all contraceptive users who fulfill study criteria. Data ' collected were socio-demographic, eating paucrn, nutritional intake (macro nutrient and folic acid), nutritional status, serum and RISC folic acid level.

Results: Serum folic acid level of < 3 mg/ml, was not found, Scrum folic acid level was not associated with using pill or non pill, socio-demographic, eating pattern, nutritional intake and nutritional status. The percentage of RISC folic acid level of N 160 mg/ml, was 3.5%. RISC folic acid level was not associated with using pill or non-pill, but there was a tendency Chef ItBC folic acid level in pill users was lower than non-pill users, There was a significant correlation between R13C folic acid level wish the frequency of green vegetables consumption and age group, 'Ihe difference in the manner of cooking significantly associated with the number of subject with RBC folic acid level of 160 mg/ml. Nutritional intake was under RDl level in almost subjects. Pill users had in average significant lower folic acid intake compare to non-pill users, but there was no correlation between folic acid intake with scrum and RBC folic acid level.

Conclusions: Serum and RHC folic acid stains is not associated with using pill or non-pill. From this study it is still not necessary for folic acid supplementation, especially for pill users."

"Latar Belakang. Penelitian ini bertujuan untuk membakukan teknik pengukuran asam folat serum menggunakan protein ikat folat PIF yang diisolasi dan dimurnikan secara utuh dari susu sapi segar dengan teknik enzyme labeled protein ligand binding assay ELPLBA . Metode. Metode yang digunakan adalah metode eksperimen yang menguji validitas dan perbandingan teknik ELPLBA untuk pembakuan teknik pengukuran folat serum.Hasil. Hasil isolasi dan pemurnian PIF menghasilkan kadar 3 tiga mg/mL. Identifikasi SDS-PAGE dan Western blot menunjukkan 3 tiga pita protein yang diperiksa adalah protein ikat folat. Validitas teknik uji keterulangan menunjukkan nilai yang dapat diterima CV.

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Background. This study was aimed to standarized technique of folic acid level serum measurment using folate binding protein FBP that isolated and purified from fresh bovine milk with enzyme labeled protein ligand binding assay ELPLBA technique. Method in this study, we performed an experiment research validated dan compared measurement techniques of ELPLBA with competitive ELISA for the standardization of the serum folic acid measurement technique. Results. FBP concentration yielded from isolated and purification was resulted 3 mg mL. SDS PAGE and western blot result showed 3 three protein bands that was confirmed to be FBP. Validity test repeatability indicate an acceptable CV 10 , whereas reproducible test showed poor results over a 5 day period. The results of the accuracy test of the enzyme labeled protein ligand binding assay technique showed good accuration. Linearity test of two samples showed quite linear results. Comparison of folic acid level measurement in serum between ELPLBA and ELISA technique showed there is no difference between both technique based on independent test T test at 95 confidence level. Conclusion. the enzyme labeled protein ligand binding assay technique on serum folic acid measurements were quite valid dan equivalent to the results obtained by competitive ELISA techniques."