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Ditemukan 13 dokumen yang sesuai dengan query
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D.P. Rahayu
The effect of gamma radiation on microbial content and curcuminoids of curcuma amada roxb. rhizomes / D.P. Rahayu, F.C. Saputri, D. Darwis
Abstrak :
The microbial contamination in the rhizomes of medicinal plants including Curcuma amada rhizomes is generally high. This due to the fact that rhizomes are the bottom parts that grow in the soil. Based on the Regulation of Head of the Indonesian National Agency of Drug and Food Control Number HK.00.06.1.52.4011, the limits of microbial contamination in herbal/medicinal plants are 106 cfu/g for the total microbial and 2×104 cfu/g for the total yeast and mold. Gamma irradiation is one of the methods to reduce microbial contamination in medicinal plants. In this research, the effectiveness of gamma irradiation in microbial reduction and its effects to curcuminoid contents was determined by irradiating Curcuma amada rhizomes at doses of 5 and 10 kGy. The initial contamination in this rhizome was 8.78×107 cfu/g and 5×101 cfu/g for the total microbial and for the total yeast and mould, respectively. The result indicates that at 5 kGy, the microbial contamination and the mould and yeast contamination were reduced from 8.78×107 cfu/g and 5×101 cfu/g to 1.39×104 cfu/g and under 1×101 cfu/g, respectively. Meanwhile the comparison of curcuminoids between the irradiated and non irradiated samples was performed by HPLC method and was found to actually increase from 0.26% to 0.36% after the 5-kGy irradiation. It can be concluded that an irradiation dose of 5 kGy is effective to reduce the content of microorganisms without lowering curcuminoids. Gamma radiation could be used as decontamination method in medicinal plants.
Center for Informatics and Nuclear Strategic Zone Utilization, 2016
607 AIJ 42:2 (2016)
Artikel Jurnal  Universitas Indonesia Library
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Muhammad Imam Surya
Pengaruh radiasi sinar gamma terhadap keragaman genetic sorgum manis (sorghum bicolor L.)
Abstrak :
ABSTRAK
Sweet sorghum is a kind of sorghum that contains high content of sugar in its stem. Sweet sorghum has a big potential to be developed in Indonesia owing to its wide adaptation and the fact that it can be used as raw material for liquid sugar, syrup, ethanol, and also as animal feed. Sweet sorghum has not been developed in Indonesia because of lack of a sweet sorghum variety. Improvement of available sweet sorghum genotype can be done among others through plant breeding program. First step on the plant breeding program is to increase the plant genetic variability. This might be done by introduction of varieties or by breeding to create new varieties. Induced mutation using Gamma irradiation can be used to increase the genetic variability of sweet sorghum. Mutation breeding using Gamma irradiation in sweet sorghum was aimed at improving the yield and quality of sweet sorghum. This research was conducted to study the effect of Gamma irradiation on sweet sorghum growth in the M1 generation, and to estimate the optimal dose range suitably for the breeding program. Beside, the objective of this research was to evaluate the genetic variability for the purpose of plant selection in the M2 generation. Plant materials consisted of 2 sweet sorghum lines introduced from ICRISAT namely line No. 79 and No. 83. Non-saccharin sorghum of local variety Fiigari was used as a control. The doses of Gamma irradiation treatment were 0, 100, 200, 300, 400, 500, 600, 700, 800, 900, and 1000 Gy. The Ml plants were sown in greenhouse at PATIR-BATAN Jakarta, and then were transplanted in the experimental field at Balitbiogen, Bogor. The M2 plants were grown in the experimental field at Lubang Buaya, Jakarta. Important agronomic traits such as plant height, spike length, stem diameter, and grain weight/spike were observed. The results indicated that sorghum lines gave different response to Gamma irradiation, and all measured variables were significantly affected. Irradiation gave morphology and physiology damages on sorghum like abnormality, sterility, and lethality in the Ml generation. The increase of irradiation doses increased physiological damage. Effective doses of Gamma irradiation for sweet sorghum was to be around 400-500 Gy, and the lethal doses 50% of sweet sorghum was around 800-1000 Gy. Putative mutation sometimes could be observed in the M2 generation. The treatment of Gamma increased genetic variability of plant height, spike length, stem diameter, and grain weight/spike. The highest genetic variability was found in the dose treatment of 200-300 Gy. Within this interval dose, there might be high probability to find desirable mutants for further breeding purpose. A number of 38 plants had been selected from the M2 population as putative mutants.
2007
T20182
UI - Tesis Membership  Universitas Indonesia Library
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Muhammad Imam Surya
Pengaruh radiasi sinar gama terhadap keragaman genetik sorgum manis (srghum bicolor L.)
Abstrak :
ABSTRAK
Sweet sorghum is a kind of sorghum that contains high content of sugar in its stem. Sweet sorghum has a big potential to be developed in Indonesia owing to its wide adaptation and the fact that it can be used as raw material for liquid sugar, syrup, ethanol, and also as animal feed. Sweet sorghum has not been developed in Indonesia because of lack of a sweet sorghum variety. Improvement of available sweet sorghum genotype can be done among others through plant breeding program. First step on the plant breeding program is to increase the plant genetic variability. This might be done by introduction of varieties or by breeding to create new varieties. Induced mutation using Gamma irradiation can be used to increase the genetic variability of sweet sorghum. Mutation breeding using Gamma irradiation in sweet sorghum was aimed at improving the yield and quality of sweet sorghum. This research was conducted to study the effect of Gamma irradiation on sweet sorghum growth in the M1 generation, and to estimate the optimal dose range suitably for the breeding program. Beside, the objective of this research was to evaluate the genetic variability for the purpose of plant selection in the M2 generation. Plant materials consisted of 2 sweet sorghum lines introduced from ICRISAT namely line No. 79 and No. 83. Non-saccharin sorghum of local variety Higari was used as a control. The doses of Gamma irradiation treatment were 0, 100, 200, 300, 400, 500, 600, 700, 800, 900, and 1000 Gy. The M1 plants were sown in greenhouse at PATIR-BATAN Jakarta, and then were transplanted in the experimental field at Balitbiogen, Bogor. The M2 plants were grown in the experimental field at Lubang Buaya, Jakarta. Important agronomic traits such as plant height, spike length, stem diameter, and grain weight/spike were observed. The results indicated that sorghum lines gave different response to Gamma irradiation, and all measured variables were significantly affected. Irradiation gave morphology and physiology damages on sorghum like abnormality, sterility, and lethality in the M1 generation. The increase of irradiation doses increased physiological damage. Effective doses of Gamma irradiation for sweet sorghum was to be around 400?500 Gy, and the lethal doses 50% of sweet sorghum was around 800?1000 Gy. Putative mutation sometimes could be observed in the M2 generation. The treatment of Gamma increased genetic variability of plant height, spike length, stem diameter, and grain weight/spike. The highest genetic variability was found in the dose treatment of 200?300 Gy. Within this interval dose, there might be high probability to find desirable mutants for further breeding purpose. A number of 38 plants had been selected from the M2 population as putative mutants.
2007
T20182
UI - Tesis Membership  Universitas Indonesia Library
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Susanto
Pengaruh Iradiasi Gamma Terhadap Kandungan Kuersetin dan Khasiatnya pada Ekstrak Etanol Bunga Rosela (Hibiscus sabdariffa L.) = The Effect of Gamma Irradiation on Quercetin and Efficacy in Ethanol Extract of Roselle (Hibiscus sabdariffa L.)
Abstrak :
Iradiasi gamma banyak digunakan oleh beberapa industri obat herbal sebagai metode pengawetan yang efisien dalam mengurangi kontaminasi mikroorganisme. Tujuan dari penelitian ini adalah untuk mengevaluasi pengaruh iradiasi gamma (0; 5; 7.5; and 10 kGy) pada ekstrak etanol H. sabdariffa (EEHS) terhadap uji total mikroorganisme, total kandungan fenol dan flavonoid, aktivitas antioksidan, TLC profiling, total kuersetin dan bobot molekul, aktivitas penghambatan terhadap enzim α-glukosidase dan in-vitro analisis terhadap galur sel kanker manusia (A-549,HUT-78, dan MCF-7). Iradiasi dosis 5 kGy menunjukkan bahwa tidak adanya kapang yang tumbuh dan terjadi penurunan jumlah total bakteri, lebih lanjut iradiasi pada dosis 10 kGy tidak terjadi pertumbuhan bakteri. Analisis total fenol, dan flavonoid, serta aktivitas antioksidan menunjukkan adanya penurunan sebesar 5-11% setelah diiradiasi pada dosis 5 kGy. Analisis profil TLC dan HPLC menunjukkan bahwa salah satu senyawa dalam EEHS adalah kuersetin yang ditunjukan dengan adanya [M+H]+ pada m/z 303,04 dari hasil analisis LC-Ms/Ms. EEHS juga memiliki penghambatan terhadap aktivitas enzim α-glukosidase dengan nilai penghambatan 4,75-7,55%. Uji aktivitas anti kanker terhadap galur sel kanker manusia menunjukkan bahwa EEHS memiliki kemampuan menginhibisi sel kanker sangat kuat dengan nilai IC50 < 20 µg/mL. Lebih lanjut, khasiat anti kanker paling kuat terhadap HUT-78, dengan nilai IC50 10,51 µg/mL, diikuti terhadap MCF-7 (IC50 13,39 µg/mL), dan  A-549 (IC50 14,19 µg/mL). Diketahui pula bahwa iradiasi dosis 5-10 kGy mampu menurunkan aktivitas anti kanker, namun penurunan tersebut tidak menghilangkan aktivitasnya yang ditandai nilai IC50 < 20 µg/mL. Berdasarkan data yang diperoleh, dapat disimpulkan bahwa iradiasi gamma dapat digunakan sebagai pengawetan pada ekstrak etanol H. sabdariffa Linn. ......Gamma irradiation is widely used by many herbal medicine industries as an efficient preservative method in reducing microorganism contamination. The purpose of this study was to evaluate the effect of gamma irradiation (0; 5; 7.5; and 10 kGy) on the ethanol extract of H. sabdariffa (EEHS) toward the total microorganism test, total phenol and flavonoid contents, antioxidant activity, TLC profile, total quercetin and its molecular weight, inhibitory activity against α-glucosidase enzyme , as well as in-vitro bioassay against human cancer cell lines (A-549, HUT-78, and MCF-7). Irradiation at a dose of 5 kGy showed that no mold grew and there was a decrease in the total number of bacteria, moreover at a dose of 10 kGy there was no bacterial growth. Analysis of total phenols and flavonoids, as well as antioxidant activitiy showed a decrease of 5-11% after irradiation at a dose of 5 kGy. TLC and HPLC profile analysis showed that one of the compounds in the H. sabdariffa extract was quercetin which was indicated by the presence of [M+H]+ at m/z = 303.04 from the LC-MS/MS analysis. EEHS also had inhibitoty  activity against α-glucosidase enzyme with the inhibition value of 4.75-7.55%. Bioassay anticancer against human cancer cell lines showed that EEHS had a very strong ability to inhibit cancer cells with the IC50 value < 21 µg/mL. Furthermore, the anti-cancer properties were strongest against HUT-78 with the IC50 value of 10.51 µg/mL, followed by MCF-7 (IC50 13.39 µg/mL), and A-549 (IC50 14.19 µg/mL). It is also known that irradiation doses of 5-10 kGy could reduce anti-cancer activity, however the decrease did not eliminate its activity which was the IC50 values still lower than 20 µg/mL. Based on the data obtained, it can be concluded that gamma irradiation can be used as a preservative method for ethanol extract of H. sabdariffa Linn.
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2023
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UI - Tesis Membership  Universitas Indonesia Library
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Linda Lidwina
Pengaruh iradiasi gamma pada mutu cabe merah kering (Capsium annuum L.)
Abstrak :
Cabe merah (Capsicum annuurn L ) mempunyai rasa pedas yang sangat disukai dan mempunyai warna merah yang menarik, oleh karena itu masyarakat sudah tidak asing lagi menggunakan cabe merah dalam kehidupan sehari—harinya. Cabe merah banyak dipergunakan antara lain untuk memberi cita rasa pada makanan. Mutu cabe merah kering ditentukan oleh kepedasan dan warnanya Kepedasan disebabkan karena adanya kandungan kapsalsin, sedang warna merah disababkan karena adanya pigmen kapsantin. Untuk meningkatkan mutu cabe kering, telah dilakukan penelitian penggunaan perlakuan iradiasi dan pencelupan dalam larutan natrium bisulfit.
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 1985
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UI - Skripsi Membership  Universitas Indonesia Library
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Ernawati
Efek iradiasi gamma terhadap kadar senyawa bioaktif, aktivitas antioksidan dan cemaran mikroba daun beluntas (Pluchea indica (L.) Less.) = Effect of gamma irradiation on the bioactive content, antioxidant activity and microbial contamination of beluntas leaves (Pluchea indica (L.) Less.)
Abstrak :
Daun beluntas atau Pluchea indica (L.) Less telah lama dimanfaatkan sebagai makanan dan obat tradisional. Iradiasi sinar gamma sering digunakan untuk dekontaminasi bahan pangan maupun herbal, namun diperlukan dosis iradiasi yang tepat agar efektif dalam membunuh mikroorganisme dengan tetap memaksimalkan kadar senyawa bioaktif dan aktivitas biologisnya. Penelitian ini bertujuan untuk mengevaluasi pengaruh iradiasi gamma 60Co terhadap cemaran mikroba, kadar senyawa turunan asam kafeoil quinat, dan aktivitas antioksidan daun beluntas. Serbuk daun beluntas diiradiasi dengan sinar gamma 60Co dengan dosis serap 0, 2,5, 5, 7,5, dan 10 kGy. Angka lempeng total bakteri aerob dan angka kapang khamir diuji menggunakan metode Petrifilm pada 0 dan 3 bulan setelah iradiasi. Setelah proses maserasi dengan etanol 70%, kadar 6 senyawa turunan asam kafeoil quinat dalam ekstrak daun beluntas ditentukan dengan Kromatografi Cair Kinerja Tinggi. Aktivitas antioksidan ekstrak daun beluntas diukur dengan metode DPPH, ABTS dan ferric reducing antioxidant power (FRAP). Iradiasi gamma dosis 2,5–10 kGy efektif menurunkan angka lempeng total dan angka kapang khamir serbuk daun beluntas (p < 0,05). Kadar senyawa turunan asam kafeoil quinat dalam ekstrak daun beluntas meningkat signifikan pada dosis 5–10 kGy (p < 0,05). Sementara aktivitas antioksidan menunjukkan kenaikan signifikan pada dosis 2,5–10 kGy (p < 0,05). Diantara dosis iradiasi tersebut, dosis 10 kGy menunjukkan hasil yang terbaik. Dengan demikian, iradiasi gamma dosis 10 kGy bermanfaat dalam menurunkan cemaran mikroba sekaligus meningkatkan kadar senyawa asam kafeoil kuinat dan aktivitas antioksidan daun beluntas. ......Pluchea indica (L.) Less or beluntas leaf has long been used as food and in traditional medicine. Gamma irradiation is widely used as a decontamination method of foodstuffs and herbs. However, the appropriate dose of gamma irradiation is necessary to reduce microbial contamination while maximizing the plant’s bioactive constituents and biological activities. This study investigated the effect of gamma 60Co irradiation on the microbial burden, caffeoylquinic acid derivatives content, and antioxidant capacity of beluntas leaf. Beluntas leaf powder was exposed to gamma rays from 60Co at the absorbed dose of 0, 2.5, 5, 7.5, and 10 kGy. The total aerobic bacteria count and total yeast and mold count were investigated using the Petrifilm method at 0 and 3 months after irradiation. After a maceration of beluntas leaf with 70% ethanol, the content of six caffeoylquinic acid derivatives (CQAs) was assayed using high-performance liquid chromatography. The antioxidant capacity of the extract was determined using the DPPH, ABTS, and ferric reducing antioxidant power (FRAP) methods. Gamma irradiation at 2.5–10 kGy effectively reduced bacteria, yeast, and mold in beluntas leaf powder (p < 0.05). The levels of CQAs in beluntas leaf extract were significantly increased at 5–10 kGy (p < 0.05). Meanwhile, the antioxidant activity was enhanced significantly at 2.5–10 kGy (p < 0.05). Among the irradiation doses, 10 kGy showed the best results. Thus, gamma irradiation at 10 kGy is useful in reducing microbial contamination as well as increasing caffeoylquinic acid derivatives content and antioxidant capacity of beluntas leaf.
Depok: Fakultas Farmasi Universitas Indonesia, 2021
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UI - Tesis Membership  Universitas Indonesia Library
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Aulia Nova Kusumaningtyas
Studi Pengaruh Iradiasi Sinar Gamma Terhadap Kualitas dan Aktivitas Antiinflamasi Ekstrak Etanol 70% Jahe Merah (Zingiber officinale Roscoe var. rubrum) = Study of the Irradiation Gamma Ray Effect on the Quality and Anti-Inflammatory Activity of 70% Ethanol Extract of Red Ginger (Zingiber officinale Roscoe var. rubrum)
Abstrak :
Jahe merah telah lama dimanfaatkan sebagai salah satu obat tradisional untuk mengurangi peradangan atau inflamasi. Namun, herbal rimpang seperti jahe merah dilaporkan mengandung cemaran mikroorganisme yang relatif tinggi. Sehingga diperlukan metode sterilisasi untuk mendekontaminasi cemaran mikroorganisme tersebut. Iradiasi gamma sering menjadi pilihan untuk dekontaminasi, tetapi belum diketahui dosis yang efektif membunuh mikroorganisme dan tidak mempengaruhi kadar senyawa bioaktif serta aktivitas biologis jahe merah. Penelitian ini bertujuan mengevaluasi pengaruh iradiasi gamma terhadap cemaran mikroorganisme, kadar senyawa 6-gingerol, 8-gingerol,10-gingerol, dan 6-shogaol serta aktivitas antiinflamasi ekstrak etanol 70% jahe merah. Serbuk rimpang jahe merah terlebih dahulu diekstraksi dengan metode maserasi menggunakan etanol 70% 1:5. Ekstrak jahe merah kemudian diiradiasi dengan dosis 0, 5, 7,5, 10, dan 15 kGy. Evaluasi cemaran mikroba angka lempeng total dan angka kapang khamir dilakukan dengan metode tuang. Evaluasi kadar bioaktif dianalisis dengan metode Kromatografi Cair Kinerja Tinggi. Aktivitas antiinflamasi diukur dengan metode penghambatan denaturasi protein BSA dengan spektrofotometer UV-Vis. Efektivitas iradiasi sinar gamma dalam menurunkan angka cemaran mikroorganisme meningkat seiring meningkatnya dosis iradiasi (p<0,05). Kadar bioaktif 6-gingerol menunjukkan kadar yang paling tinggi diantara tiga senyawa bioaktif lainnya. Namun, secara keseluruhan dosis iradiasi gamma yang diberikan tidak berpengaruh signifikan terhadap kandungan 6-gingerol,8-gingerol,10-gingerol dan 6-shogaol dalam ekstrak etanol 70% jahe merah (p>0,05). Aktivitas antiinflamasi ekstrak etanol 70% jahe merah juga tidak berbeda signifikan setelah di iradiasi dengan dosis 0-15 kGy (p>0,05). ......Red ginger has long been used as a traditional medicine to reduce inflammation. However, rhizome herbs such as red ginger are reported to contain relatively high levels of contamination by microorganisms. So a sterilization method is needed to decontaminate the microorganisms. Gamma irradiation is often an option for decontamination, but it is not yet known which dose is effective in killing microorganisms and does not affect the levels of bioactive compounds or the biological activity of red ginger. This study aims to evaluate the effect of gamma irradiation on contamination of microorganisms, levels of compounds 6-gingerol, 8-gingerol, 10-gingerol, and 6-shogaol, and the anti-inflammatory activity of a 70% ethanol extract of red ginger. Red ginger rhizome powder was first extracted by the maceration method using 70% ethanol at 1:5. The red ginger extract was then irradiated at doses of 0, 5, 7.5, 10, and 15 kGy. The evaluation of microbial contamination of the total plate count and yeast mold count was carried out by the pouring method. The evaluation of bioactive levels was analyzed using the high-performance liquid chromatography method. Anti-inflammatory activity was measured by inhibiting BSA protein denaturation with a UV-Vis spectrophotometer. The effectiveness of gamma irradiation in reducing the number of microorganisms contaminating the environment increased with increasing irradiation dose (p 0.05). The level of bioactive 6-gingerol showed the highest level among the other three bioactive compounds. However, overall, the dose of gamma irradiation given had no significant effect on the content of 6-gingerol, 8-gingerol, 10-gingerol, and 6-shogaol in the 70% ethanol extract of red ginger (p > 0.05). The anti-inflammatory activity of the 70% ethanol extract of red ginger was also not significantly different after irradiation with dose 0-15 kGy (p > 0.05).
Depok: Fakultas Farmasi Universitas Indonesia, 2023
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UI - Tesis Membership  Universitas Indonesia Library
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Fitria Ramadhani
Pengaruh iradiasi sinar gamma pada herba suruhan (peperomia pellucida (L.) kunth) terhadap penghambatan aktivitas angiotensin converting enzyme (ACE) = Effect of gamma irradiation in (peperomia pellucida (L.) kunth) on inhibition of angiotensin converting enzyme (ACE)
Abstrak :
Iradiasi sinar gamma merupakan teknologi efektif yang dapat digunakan untuk mengurangi kontaminan pada simplisia. Peperomia pellucida (L.) Kunth termasuk suku piperaceae yang memiliki aktivitas sebagai ACE inhibitor. Penelitian ini bertujuan untuk menganalisis pengaruh iradiasi sinar gamma terhadap jumlah mikroba, aktivitas ACE inhibitor dan perubahan komponen kimia dari herba suruhan (Peperomia pellucida (L.) Kunth). Herba suruhan diiradiasi dengan berbagai dosis 0;2,5; 5;7,5 dan 10 kGy kemudian diekstraksi menggunakan metode refluks dengan etanol 80% selama 30 menit. Jumlah mikroba dihitung menggunakan petrifilm aerobic count plate. Penghambatan aktivitas ACE diukur menggunakan ACE kit WST dan perubahan kimia dilihat melalui profil kromatografi lapis tipis menggunakan lempeng silica gel f254 sebagai fase diam dan campuran diklorometana:metanol (90:10) sebagai fase gerak. Iradiasi sinar gamma hingga dosis 7,5 kGy mengurangi cemaran mikroba hingga 2x102±0,00 koloni/gram. Aktivitas ACE inhibitor meningkat secara signifikan (p<0,05) setelah diiradiasi sinar gamma, namun iradiasi sinar gamma menyebabkan degradasi pada senyawa flavonoid yang terdapat pada herba suruhan.
Gamma irradiation is an effective technique can be used to reduce contaminants in herbal products. Peperomia pellucida (L.) Kunth belongs to piperaceae family has activity as ACE inhibitor. The aimed of this research were to determine the effects of gamma irradiation on microbial load, ACE inhibition activity and change of chemical compounds of P. pellucida (L.) Kunth). Sample was irradiated at various dose of 0, 2.5, 5, 7.5 and 10 kGy and extracted by reflux method using 80% ethanol for 30 minutes. Microbial load was counted by using petrifilm aerobic count plate method. The ACE inhibition activity was tested by using ACE kit WST and change of chemical compounds, was examined by using thin layer chromatography profiling, used silica gel f254 plate as stationary phase and dichloromethane-methanol (90:10) mixture as eluent. Gamma irradiation up to 7,5 kGy reduced microbial load up to 2x102±0,00 cfu/g. ACE inhibition activity significantly increased (p<0,05) after irradiation treatment, but gamma irradiation caused degradation of flavonoid content in Peperomia pellucida (L.) Kunth.
Depok: Fakultas Farmasi Universitas Indonesia, 2016
S65249
UI - Skripsi Membership  Universitas Indonesia Library
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Aghnianditya Kresno Dewantari
Pengembangan uji serologi igm antibody-capture enzyme linked immunosorbent assay mac elisa untuk virus japanese encephalitis strain nakayama dan virus chikungunya isolat csb04010 = Development of igm antibody-capture enzyme linked immunosorbent assay mac elisa for japanese encephalitis virus strain nakayama and chikungunya virus isolate csb04010 / Aghnianditya Kresno Dewantari
Abstrak :
ABSTRAK
Arbovirus (arthropode-borne virus) yang timbul dan timbul kembali telah memengaruhi berbagai aspek kehidupan manusia. Infeksi arbovirus terbanyak di Indonesia: dengue, Japanese encephalitis (JE) dan chikungunya (CHIK) menyebabkan kasus luar biasa tiap tahun. Ketersediaan metode deteksi JE dan CHIK sangat terbatas di Indonesia. Pengembangan in-house IgM antibody-capture Enzyme Linked Immunosorbent Assay (MAC ELISA) dengan antigen local terinaktivasi akan meningkatkan deteksi dan pemantauan dengan meningkatkan spesifisitas dan sensitivitas. Antigen diproduksi dalam kultur sel dengan sel BHK-21 dan sel Vero kemudian diinaktivasi dengan gamma-irradiasi dan 0,01% beta-propiolakton. Kinerja Antigen dievaluasi dengan uji MAC ELISA dan titer virus dihitung dengan uji plak. Virus Japanese encephalitis dan chikungunya terinaktivasi pada 20 kGy gamma- irradiasi dan 0,01% BPL. In-house MAC ELISA telah dioptimisasi dengan inkubasi 2 jam. Kit in-house MAC ELISA yang telah dikembangkan berguna untuk deteksi dan pemantauan JE dan Chik dengan fasilitas terbatas.
ABSTRACT
The emerging and re-emerging arthropod-borne viruses (arboviruses) have effected many aspects of human existence. Three major arbovirus infection in Indonesia: dengue, Japanese encephalitis (JE) and chikungunya (CHIK) causes numerous outbreaks each year. However, availability of detection methods for JE and CHIK are very limited in Indonesia. Development of in-house IgM antibody-capture Enzyme Linked Immunosorbent Assay (MAC ELISA) with inactivated local antigen will improve detection and surveillance capability across Indonesia by increasing its specificity and sensitivity. Antigens were produced in cell culture using BHK-21 cells and Vero cells then inactivated using gamma-irradiation and 0.01% beta-propiolactone (BPL). Antigen performance was evaluated using MAC ELISA and virus titer were calculated using plaque assay. Japanese encephalitis virus and chikungunya virus was inactivated at 20 kGy with 0.01% BPL. Optimized in-house MAC ELISA protocol using these antigen has been developed. Developed in-house MAC ELISA kit will be beneficial for detection and surveillance of JE and CHIK with limited facility.
2016
S65193
UI - Skripsi Membership  Universitas Indonesia Library
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Agung Kristiyanto
Pengaruh iradiasi gama pada biji melinjo (gnetum gnemon l.) terhadap aktivitas penghambatan HMG-KoA reduktase dan angka lempeng total = Effect of gamma irradiation on HMG-CoA reductase inhibitory activity and total plate count of melinjo (gnetum gnemon l.) seeds
Abstrak :
Ekstrak biji melinjo Gnetum gnemon L. diketahui berperan dalam aktivitas penghambatan HMG-KoA reduktase. Sedangkan iradiasi adalah salah satu metode dalam sterilisasi bahan alam. Studi ini bertujuan untuk mengetahui pengaruh iradiasi gama terhadap aktivitas penghambatan HMG-KoA reduktase dan angka lempeng total pada biji melinjo. Biji melinjo diiradiasi dengan berbagai dosis yaitu 0, 2,5, 5, 7,5, dan 10 kGy. Ekstraksi dilakukan dengan menggunakan metode refluks dengan pelarut etanol. Angka lempeng total ALT ditentukan menggunakan metode aerobic count plate melalui pengenceran suspensi serbuk biji melinjo. Sementara uji aktivitas penghambatan HMG-KoA reduktase menggunakan assay kit HMG-KoA reduktase. Dalam uji aktivitas penghambatan HMG-KoA reduktase, diperoleh persentase penghambatan oleh standar pravastatin sebesar 97,41 dengan IC50 76,70 nM. Ekstrak biji melinjo berbagai dosis iradiasi diuji aktivitas penghambatannya dan diperoleh persentase penghambatan tertinggi sebesar 97,30 pada dosis iradiasi 2,5 kGy. Pada penetapan ALT, serbuk biji melinjo berbagai dosis iradiasi menunjukkan tidak adanya pertumbuhan mikroba. Berdasarkan penelitian ini, dapat disimpulkan bahwa biji melinjo berada dalam kondisi steril dan iradiasi 2,5 kGy adalah dosis optimum untuk menghambat HMG-KoA reduktase secara signifikan.
Melinjo Gnetum gnemon L. seed extract is known to play a role in the inhibitory activity of HMG CoA reductase. Irradiation is a method to sterilize natural products. This study aimed to determine the effect of gamma irradiation on the inhibitory activity of HMG CoA reductase and total plate count TPC . Melinjo seeds were irradiated with various doses of 0, 2.5, 5, 7.5, and 10 kGy. The extraction was carried out by ethanol using reflux method. TPC was determined by aerobic count plates method using stock dilution of melinjo seeds powder suspensions. HMG CoA inhibitory activity was determined using HMG CoA reductase assay kit. In determination of HMG CoA reductase, the inhibitory percentage of pravastatin standard was 97.41 and the IC50 was 76.70 nM. Irradiated melinjo seed extracts were tested for inhibitory activity and the highest inhibition percentages were 97.30 of 2,5 kGy. In the determination of TPC, the powder of melinjo seeds for all irradiation doses showed no microbial growth. Based on this research, it can be concluded that non irradiated and irradiated melinjo seeds were free from microbial growth and the gamma irradiation dose of 2.5 kGy was optimum dose to inhibit HMG CoA reductase, significantly.
Depok: Fakultas Farmasi Universitas Indonesia, 2017
S69840
UI - Skripsi Membership  Universitas Indonesia Library
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