Hasil Pencarian  ::  Simpan CSV :: Kembali

"Banyak orang berusaha untuk menurunkan maupun menjaga agarberat badan ideal. Tujuan penelitian ini untuk mengetahui pengaruhpemberian jamu pelangsing "SF" pada tikus putih selama 90 hari terhadaporgan ginjal ditinjau dari kadar urea dan histologis ginjal. Hewan coba yangdigunakan tikus putih jantan dan betina galur Sprague-Dawley berumur 3-4bulan dengan berat badan 200-300 g. Pada hari ke-91 tikus diambil darahnyamelalui mata dan dibedah untuk diambil ginjalnya. Selanjutnya dilakukanpengukuran kadar urea plasma dengan metode kolorimetri serta histologisginjal. Kadar rata-rata urea plasma kelompok perlakuan berkisar 31-37 mg/dl dankelompok kontrol 34-37 mg/dl. Diameter rata-rata glomerulus ginjal kelompokperlakuan berkisar 0,88-0,96 μm dan kelompok kontrol 0,93 μm. Jarak ruangrata-rata antara glomerulus dengan kapsula Bowman berkisar 0,09-0,11 μm dankelompok kontrol 0,1 μm. Hasil uji Oneway ANOVA pada
=0,05 terhadap kadarurea plasma, diameter glomerulus ginjal dan jarak ruang antara glomerulusdengan kapsula Bowman tidak menunjukkan perbedaan bermakna baik antarkelompok perlakuan maupun dengan kelompok kontrol. Penggunaan sediaanjamu pelangsing "SF" selama 90 hari tidak mempengaruhi fungsi organ ginjaltikus putih."

"This study was carried out to investigate the effect of 4NQO oral induction in oesophagus of male rat. Sixteen male Sprague Dawley rats were divided into three experimental groups and one untreated group as control. The experimental groups were applied with 0.5% 4-nitroquinoline 1-oxide on the dorsal mucosa of tongue thrice weekly for 8, 16 or 24 weeks, one brush stroke per application. At the end of the 36th week, all rats were sacrificed and the tongue and oesophagus were excised and fixed in 10% buffed formalin for 24 hours. The H&E sections were prepared for histological examination. The microscopial assessment showed that all rat tongues whether applied with 4NQO for 8, 16 or 24 weeks were identified having Squamous Cell Carcinoma (SCC). Microscopial examination of oesophagus indicated that 75% or the rats applied with 4NQO for 16 weeks showed hyperkeratosis, and 80% and 20% of the rats applied with 4NQO for 24 weeks showed malignancy changes and hyperkeratosis, respectively. No histological changes were detected either in the tongue or the oesophagus of the control rats. It was concluded that the effect of carcinogenic induction in oral mucosa caused malignant changes in oesophagus."

"Abstract:"Covering the science behind the disease a comprehensive approach to modern caries management. This systematic approach to modern caries management combines new, evidence-based treatment techniques with the scientific underpinnings of caries formation providing an in-depth review for both clinicians in daily practice and students advancing in the field. Beginning with patho-anatomic changes in the dental hard tissues, Dental Caries: Science and Clinical Practice goes on to cover non-invasive, minimally invasive, and more aggressive interventions based on each stage of the disease. From microbiology and histology to visual, tactile and radiographic diagnosis, risk assessment, preventive measures, and tooth preservation and treatment strategies, the book is packed with valuable clinical information for all dental practitioners. Succinctly covers the science behind the disease, with recommendations for treatments based on assessment starting at the microscopic level Written by a team of leading worldwide authorities on caries treatment and managementand utilizing the International Caries Detection and Assessment System (ICDAS) standard throughout Covers the newest treatment techniques, including adhesion technology, fissure sealing and infiltration, caries removal, tooth-colored restorations, and more Demonstrates step-by-step caries procedures in striking, full-color illustrations of adult and pediatric cases Offers the newest thinking on early prevention and behavioral changes in oral health promotion, including the role of diet and nutrition, biofilm management, fluoride use, population-based approaches, and more. Shifting to the new paradigm of heal and seal rather than the more invasive drill and fill, this beautifully illustrated text puts scientific principles into clinical action for the best results. It is an essential resource for a complete, proactive approach to caries detection, assessment, treatment, management, and prevention in contemporary dental practice"--Résumé de l'éditeur."

"LS drug is a synthetic of statin group that has been used as an antihyperlipidemia. The LS drug has been used long-term repeated administration must be assessing especially for liver function of rat through alanine aminotransferase (ALT) activity plasma and histological structure. This study used Sprague Dawley rats that is divided into 4 groups, each one consist of 10 male and female rats. Group I, II, III were given statin with 1,8; 3,6; 7,2mg/ 200g bw dosage as a study groups, while group IV was given CMC 0,5% orally as a control group. The studies have been finished for 90 days and 91st day, the blood samples were taken from orbital sinus of eye, and the liver was taken to observe its histology. The ALT plasma activities were measured with colorimetric method (Reitman-Frankel) and histological structure were colored by paraffin method and Haematoxylin-Eosin stain by further central vein diameters was measured. One way varian analysis of ALT plasma activities and central vein diameter (α = 0,05) showed that there was no significant differences between studies groups and control group. The result was indicated that given LS drug for 90 days did not have sigificant effect on the liver function.

---

Obat LS merupakan obat sintesis golongan statin yang digunakan sebagai antihiperlipidemia. Obat LS digunakan berulang dan dalam jangka panjang sehingga perlu diketahui pengaruhnya terhadap fungsi hati, dengan menilai aktivitas Alanin aminotransferase (ALT) plasma dan gambaran histologi hati. Penelitian menggunakan tikus putih jantan dan betina galur Sprague Dawley yang dibagi ke dalam empat kelompok masing-masing 10 ekor. Kelompok I, II, III adalah kelompok perlakuan yang diberi larutan uji dengan dosis berturut-turut 1,8; 3,6; dan 7,2mg/ 200g bb perhari. Kelompok IV adalah kelompok kontrol yang diberikan larutan CMC 0,5%. Penelitian dilakukan selama 90 hari dan pada hari ke-91 sampel darah tikus diambil melalui sinus orbital mata kemudian dibedah untuk diambil hatinya. Pengukuran aktivitas ALT plasma menggunakan metode kolorimetri (Reitman-Frankel) serta histologi hati dengan metode parafin dan pewarnaan hematoksilin-eosin kemudian dilakukan pengukuran terhadap diameter vena sentralis. Hasil ANAVA (α=0,05) terhadap ALT plasma dan pengukuran diameter vena sentralis tidak menunjukkan perbedaan bermakna antar kelompok perlakuan maupun kelompok kontrol. Hasil menunjukkan bahwa pemberian obat LS selama 90 hari tidak mempengaruhi fungsi hati."

"ABSTRAKPendahuluan: Fusi spinal posterolateral adalah prosedur sering dilakukan dalam tindakan arthrodesis spinal yang menggunakan autograft dari krista iliaka. Penggunaan autograft memiliki keterbatasan dan komplikasi. Tingkatpseudoarthrosis berkisar antara 5-35%. Penggunaan platelet rich plasma (PRP) sebagai faktor osteoinduktif memiliki dasar ilmiah. Platelet dengan cepatmenempel pada permukaan tandur tulang dan mengalami degranulasi, kemudian melepaskan faktor-faktor pertumbuhan yang memicu penyembuhan tulang dan inkorporasi tandur. Studi ini bertujuan untuk mengetahui efek pemberian PRP terhadap tingkat fusi pada tindakan fusi spinal pada kelinci putih New Zealand Metode: Fusi posterolateral dilakukan pada 16 ekor kelinci putih New Zealand yang terbagi dalam 2 kelompok, yaitu kelompok fusi dengan autograft dan fusi dengan autograft + PRP. PRP diambil dari vena perifer lalu disentrifugasi, kemudian dilanjutkan dengan tindakan fusi posterolateral, pengambilan tandur krista iliaka, dan aplikasi PRP. Observasi dilakukan selama 8 minggu. Evaluasi dinilai secara radiologis dengan modifikasi skor Bridwell dan histologis dengan skor Huo/Friedlaender.Hasil: Secara radiologis, pada kelompok perlakuan fusi spinal dengan autograftdidapatkan 5 sampel definite fusion, 1 sampel probable fusion, dan 2 sampelnonunion. Sementara itu, pada kelompok perlakuan fusi spinal dengan autograft + PRP terdapat 5 sampel definite fusion, 3 sampel probable fusion, dan tidak ada nonunion. Namun, tidak terdapat perbedaan radiologis yang bermakna. Kelompok fusi spinal dengan autograft + PRP memiliki rerata dan median skor histologis yang lebih tinggi dibandingkan kelompok fusi spinal dengan autograft saja secara bermakna (8 vs 6,5).Simpulan: Pemberian PRP bersama dengan autograft pada fusi spinalposterolateral memiliki pengaruh terhadap tingkat fusi pada fusi spinal padakelinci putih New Zealand. Pemberian PRP dapat dipertimbangkan dan ditelitilebih lanjut sebagai faktor osteoinduktif alternatif dalam fusi spinal posterolateral

---

ABSTRACTIntroduction: Posterolateral spinal fusion is a common procedure in spinal arthrodesis using autograft from iliac crest. Autografts utilization possess limitations and complication with pseudoarthrosis ranging between 5-35%. Platelet rich plasma (PRP) usage as an osteoinductive factors is based on scientific reasons. Platelets can quickly adhere to the surface of the bone graft and degranulate, releasing growth factors afterwards and inducing bone healing and graft incorporation. This study aim to establish the effect of PRP administration on fusion rate for spinal fusion on New Zealand white rabbit.Method: Posterolateral fusions were done on 16 white New Zealand rabbits divided into two groups: fusion with autograft and fusion with autograft + PRP. PRP was taken from peripheral vein and centrifuged. Posterolateral fusion and graft harvesting from iliac crest were followed by PRP application. Observation was done for 8 weeks. Evaluation was done radiologically with modified Bridwell score and histologically with Huo/Friedlaender score.Results: Radiologically, for spinal fusion with autograft group, the result were 5 definite fusion, 1 probable fusion, and 2 nonunion fusion. For spinal fusion with autograft + PRP, there was 5 definite fusion, 3 probable fusion, and no nonunion. There was no significant difference radiologically. Spinal fusion with autograft + PRP had higher mean and median for histological score compared to spinal fusion with autograft (8 vs 6.5).Conclusion: PRP administration with autograft for posterolateral spinal fusion affected fusion rate for spinal fusion on New Zealand white rabbit. PRP administration can be considered and studied further as an alternative for osteoinductive factors for posterolateral spinal fusion"

"ABSTRAKPendahuluan: Alfa fetoprotein AFP merupakan penanda tumor yang mengalami peningkatan pada karsinoma hepatoselular KHS namun dapat juga normal pada 40 kasus. Peningkatan AFP dikatakan menghasilkan diferensiasi tumor yang buruk. Kasus KHS yang datang ke RSCM cenderung lanjut dengan karakteristik tersendiri. Penelitian ini bertujuan menilai korelasi AFP terhadap derajat diferensiasi KHS di RSCM.Metode: Data 32 kasus KHS di RSCM yang dilakukan hepatektomi dikumpulkan secara retrospektif dari 2010-2016. Data dasar karakteristik pasien dinilai berdasarkan nilai AFP dan derajat diferensiasi. Lalu dilakukan analisis untuk melihat korelasi nilai AFP dengan derajat diferensiasi KHS.Hasil: Nilai rata-rata AFP adalah 20183 ng/mL, usia 51,75 tahun, 81 kasus terjadi pada laki-laki, 59,4 berdiferensiasi buruk, 50 berukuran >5-10 cm, 50 kasus sirosis, dan 68,8 terdapat invasi pembuluh darah. Diferensiasi buruk 42 pada laki-laki, 47,4 pada HbsAg positif, 50 pada Anti HCV positif, 31,2 sirosis, dan 40,9 mengalami invasi pembuluh darah. Nilai AFP 20 ng/mL 42,1 berdiferensiasi buruk. Pada analisis orelasi didapatkan r=0,203 dengan p>0,05.Kesimpulan: AFP tidak dapat memprediksi derajat diferensiasi karsinoma hepatoseluler pada karakteristik kasus KHS di tempat kami.

---

ABSTRACT<>br>Background AFP is a tumor marker which is increased in HCC, but might be found normal in 40 cases. Increased AFP implies a worsen tumor differentiation. Correlation between AFP with HCC managed in RSCM Ciptomangunkusumo hospital remains unclear. This study aimed to find the correlation between histological differentiation grade of HCC and AFP serum level.Method A total of 32 of HCC cases following hepatectomy in RSCM during 2010 2016 were enrolled in a retrospective study. Subject characteristics, AFP levels and histological differentiation grade were the variables in this study, and subjected to statistical analysis. Significancy found if p 5 10cm in diameter, 50 subjects were cirrhotic, and 68.8 subjects with microvascular invasion. AFP level found in range of 0.5 400000 ng mL 20183 SD75580.08 . Among all the subjects 12.5 were well differentiated, 28.1 were moderate differentiated, and 59.4 were poorly differentiated. AFP level 20ng mL was 42.1 . Correlation analysis revealed an r 0.203 with p 0.05.Conclusion There is no correlation between AFP serum level and histological differentiation grade of HCC in our study"

"Coconut is a very important plant for the livelihood of people in tropical countries. It is also used as an icon of tropical region. Coconut fruit is very heavy and can cause injuries if the fruit falls down and hits somebody who happens to be underneath a coconut tree. In order to avoid the accident,  the coconut fruits have to be regularly cut off. Coconut tree originated from in vitro cultured endosperm is a triploid plant that produces seedless fruit (without endocarp). Coconut fruit without endocarp is not heavy. The objective of this study was to investigate plant regeneration of fresh and in vitro cultured coconut endosperms. The fresh and developed in vitro cultured coconut endosperms were observed using histological analysis. Solid endosperm of seven month-old postanthesis coconut from “Samoan Dwarf†cultivar was freshly picked up and cultured in vitro on modified Branton & Blake formula. Histological study of fresh coconut endosperm showed that the endosperm consisted of parenchyma cells, which were relatively uniform in shape and size, with some nuclei consisted of 1 – 5 nucleoli. Three month-old calli of in vitro grown coconut endosperm in semi solid media showed that its cells varied in shape and size, characterized by high nucleus to cytoplasm ratio, high starch, protein and lipid contents which underwent many divisions. Seven month-old calli of in vitro grown coconut endosperm in liquid media showed embryogenic cells which resembled proembryos. Fourteen month-old bud-like structure of coconut endosperm in semi solid media showed a meristematic layer, tunica-corpus structure, cortex-like region and tracheids of xylem. These results indicated that the bud-like structure was an early stage of shoot bud formation in coconut endosperm. This is the first report of early stage of shoot bud formation occurring on coconut endosperm cultured in vitro."

"Telah dilakukan penelitian laboratorium untuk mengetahui pengaruh pencekokan zat warna C.I. Food Yellow 4 terhadap gejala klinik dan perubahan struktur histologi organ ginjal mencit (Mus musculus L.). Pencekokan dilakukan terhadap 24 ekor mencit yang dibagi dalam 4 kelompok perlakuan dengan dosis : 0,00 (1 ); 5,25 (11); 7,25 (III) dan 9,25 (IV ) gram C.I. Food Yellow 4/kg berat badan mencit dengan pelarut aquabidestilata. Setelah perlakuan gejala klinik yang timbul diamati setiap hari, Pada hari ke-6 semua mencit dikorbankan dengan cara dislokasi serviks dan diambil organ ginjalnya untuk dibuat preparat Histologi. Hasil pengamatan terhadap gejala klinik memperlihatkan bahwa pada semua kelompok perlakuan terjadi diare dan perubahan berat badan yang tidak stabil. Hasil pengujian Kruskal - Wallis pada jurnlah rata-rata kerusakan glomerulus (α = 0,05) menunjukkan adanya perbedaan nyata pada kelompok kontrol dengan semua kelompok perlakuan. Hasil pemeriksaan mikroskopik organ ginjal mencit perlakuan kelompok (II ) tampak kerusakan ringan pada bagian glomerulus. Sedangkan pada kelompok mencit perlakuan ( III ) dan (IV ) terlihat kerusakan glomerulus semakin meningkat berupa lisisnya sel-sel lapis parietal dan viseral yang mangakibatkan terjadinya atropi dan pelebaran ruang antar Bowman. Kerusakan organ ginjal tampak jelas meningkat seiring dengan kenaikan dosis zat warna C.I. Food Yellow 4 yang dicekokan.

---

A study of the effects. of a food color substance, C.I. Food Yellow 4, on clinical symptoms of mice (Mus musculus L.) and their kidneys were conducted in the laboratory. Each of four equal groups of six mice was individually fed with an artificial diet, impregnated with C.I. Food Yellow 4, at doses of 0.00, 5.25, 7.25, and 9.25 g I kg of body wieght on a daily basis.

Clinical symptoms of mice were observed every day before scarifying the mice by using a cervix dislocation technique on 6 th day of the experiment. The results showed that all treated mice underwent diarrhea and their weights fluctuated. Kruskal - Wallis test indicated that there was a significant difference (a = 0.05) of glomerulus damage between control and treated mice.

Histological examinations of kidney tissue of mice treated with 5.25 g of food color substance per kg of body weight showed that there was a slightly damage on glomeruli. Mice treated with 7.25 and 9.25 g 1 kg caused heavy damages in glomeruli. Such as parietal and visceral cells lyses which increased distance between two Bowman capsule walls. The level of kidney damage apparently increased with the increase of doses of C.I. Food Yellow 4 given."

"Background: Some studies suggested that colorectal cancer at young age had a distinct biological characteristic: more advanced stage at time of diagnosis, poorer differentiated, and consisted of large proportion of mucin producing tumors.Aim of the study: To analyze clinical and histopathological differences between young aged colorectal cancer patients (< 40 years old) and the older patientsMethods: A cross-sectional retrospective study was conducted among our colorectal cancer patients in a general hospital between 1999-2004, using C.I8, C.I9, C20 ICD X code in medical record searching. It was requested that the patients had surgical treatment in Dr. Sardjito General Hospital. An inconclusive clinical staging and/or histological data were among the exclusion criteria. Chi-square, Fisher's exact test, T-test, and Mann Whitney U-test was performed to analyze the difference between patients < 40 years old and 40 years old, in respect to diagnostic staging, histological type, histological grade (differentiation), CEA level, hemoglobin, albumin, tumor location, and chief complain. With p < 0.05 was considered as significant.Results: Sixteen young aged (< 40 years old) and 72 older patients had been identified. No differences in gender proportion and mean of symptoms to diagnosis period between two groups. No statistical differences between young aged and the older patients in diagnostic staging, histological grade and type, CEA level, and hemoglobin. Young aged patients had higher albumin value at presentation (p = 0.014), all had left sided tumors (p = 0.035), more complain of anal pain (p < 0.001), and less change of bowel habits complain (p = 0.009)Conclusion: The study results had failed to confirm the difference in respect to diagnostic staging, histological type and grade, CEA level, and hemoglobin. Most of our young aged patients had left sided tumors with chief complain of anal pain, and less complain of change of bowel habits"

"ABSTRAKPenelitian ini bertujuan untuk mendapatkan data pengaruh penghentian pajanan monosodium glutamat terhadap gambaran histologis tubuli seminiferi testis pada tikus putih (Rattus norvegicus) dewasa. Penelitian ini adalah penelitian eksperimental in vivo prospektif, menggunakan tikus putih (Rattus norvegicus) dewasa strain Sprague Dawley yang diberi msg dosis 4 g/kgBB dan 6 g/kgBB tiap hari selama 30 hari. Msg diberikan dengan dosis 4 g/kgBB dan 6 g/kgBB yang dilarutkan dalam aquades, masing-masing pada satu kelompok besar. Selain itu digunakan sekelompok besar tikus sebagai kelompok perlakuan yang hanya diberikan aquades. Perlakuan dilakukan dengan pemberian menggunakan sonde 1,5 ml aquades atau aquades 1,5 ml yang merupakan larutan msg sesuai dosis. Sehari, 14 hari dan 28 hari pasca penghentian pajanan, tikus disakrifikasi dan dilakukan pengamatan. Pengamatan dilakukan pada tubuli seminiferi testis, yaitu pada diameter tubuli seminiferi, jumlah spermatogonia, jumlah spermatosit dan jumlah spermatid. Penelitian ini menyimpulkan adanya perubahan (regenerasi) pada tubuli seminiferi, meskipun belum sampai pada keadaan seperti kontrol perlakuan. Data ini diharapkan dapat memberi informasi dan membuka kesempatan penelitian lanjut yang terkait dengan mekanisme regenerasi pasca kerusakan oleh monosodium glutamat.

---

ABSTRACTThe aim of this study was to analize histological appearance of testis after termination of msg exposure. Msg was given 4 g/kg BW and 6 g/kg BW soved ini aquadest. Each those treatment was given in two group of rats. This study also used a group of treatment control rat. The treatment was done by giving with sonde 1,5 ml aquadest or 1,5 ml aquadest with msg according the doses. The testis were isolated one day, 14 days and 28 days after termination of msg exposure; the rat were sacrificed and were examined the histological appearances of the testis. Spesificaly, the examination was done to tubuli seminiferi diameter, the amount of the spermatogonia, spermatocyts and spermatids. This study concluded that there was changed (regeneration) of the tubuli seminiferi, although not yet reach the situation in the control groups."