Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
Tujuan: Mengetahui pengaruh suplementasi vitamin B12 400 µg/hari selama enam minggu terhadap kadar vitamin B12 dan homosistein serum pada adventis vegan dewasa. Tempat: Gereja Masehi Advent Hari Ketujuh, Jakarta Barat. Metadologi: Penelitian pro dan pasca perlakuan pada 27 orang subjek, berusia 20-60 tahun. Setiap subjek mengkonsumsi suplemen vitamin B12 400 µg/hari dosis tunggal selama 42 hari. Data yang dikumpulkan meliputi data demografi, antropometri pra dan pasca perlakuan, asupan nutrisi (energi, karbohidrat, protein, dan lemak) dengan metode recall 1 x 24 jam dua kali seminggu pra, pertengahan dan pasca perlakuan. Data asupan vitamin B6, B12 dan asam folat dengan FFQ semi kuantitatif serta data laboratorium meliputi kadar vitamin B12, asam folat, dan homosistein serum pra dan pasta perlakuan. Hasil: Data demografi menunjukkan sebagian besar (81,5%) subjek berpendidikan tinggi dan semua subjek berpenghasilan di atas garis kemiskinan. Data antropometri pada pra dan pasca perlakuan menunjukkan seluruh subjek mempunyai IMT dalam batas normal. Asupan nutrisi selama perlakuan yang meliputi asupan energi, karbohidrat, lemak dan protein tidak mengalami perubahan. Bila dibandingkan dengan AKG tahun 1988 asupan energi, sebagian besar subjek termasuk cukup, asupan karbohidrat, dan protein termasuk kurang; asupan lemak termasuk lebih, Asupan vitamin B6 pada akhir perlakuan tidak menunjukkan penurunan secara signifikan (p=0,6874), sebaliknya dengan asupan vitamin B12 yang menunjukkan peningkatan signifikan (p = 0,021) dan asam folat yang menunjukkan penurunan signifikan (p = 0,0001). Hasil pemeriksaan laboratoriurn pada akhir perlakuan menunjukkan peningkatan signifikan pada kadar vitamin B12 (p = 0,0000) sebesar 202,6%, dari median 127 (58,0-193,0) pg/mL menjadi 376 (183,0-1168,0) pg/mL dan penurunan kadar homosistein yang signifikan (p = 0,0000) sebesar 39% dari median 14,50 (11,1-34,2) } µmol/L menjadi 9,50 (5,6-24,8) µmol/L. Kadar asam folat tidak mengalami penurunan bermakna (p = 0,2960). Kesimpulan: Suplementasi vitamin B12 sebanyak 400 µg/hari selama 42 hari pada vegan terbukti meningkatkan kadar vitamin B12 dan menununkan kadar homosistein. ...... The Effect Of Vitamin B12 Supplementation On Homocysteine Level Of Adult VegansObjective: To investigate the effect of 400 µg /day vitamin B12 supplementation for 42 days on serum vitamin B12, and homocysteine levels of 27 adult vegan subjects. Location: Seventh Day Adventists Church, West Jakarta. Method: A pre and post test design study was carried out on 27 subjects, aged 20-60 years, who fulfilled the criteria of the selection. Subjects were given 400 µg/day vitamin B12 single dose supplementation for 42 consecutive days. Data collected were demographic, anthropometric, nutritional, and laboratory. The data of energy, carbohydrate, protein, and fat intake were collected using 1 x 24 recall method twice a week at the beginning, within, and the end of the study; whilst vitamin B6, B12 and folk acid intake were obtained with FF0 semi-quantitative method at the beginning and the end of the study. Laboratory data were collected before and after study including serum vitamin B12, folk acid and homocysteine Results: Demographic data showed that most of the subjects had high formal education level (81.5%) and all subjects had income above the poverty line. Anthropometric data showed that BMI at the beginning and in the end of the study were in normal range. Dietary intake estimation including energy, carbohydrate, protein, and fat, were not significantly changed. Compared to Indonesian RDA 1998, intake of energy was considered adequate, carbohydrate and protein were low, and fat was high. Vitamin B6 intake did not decrease significantly (p = 0.6874) However vitamin B12 intake increased (p = 0.021) and folic acid intake decreased significantly (p = 0.0001). Median value of serum vitamin B12 after supplementation increased significantly (p = 0,0000) by 202.6% from 127 (58.0-193.0) pg/mL to 376 (183.0-1168.0) pg/mL. There was no significant difference in the serum level of folic acid (p = 0.2960). Median value of homocysteine after supplementation decreased significantly (p = 0.0000) by 39% from 14.50 (3.8-34.2) man, to 9.50 (5.6-24.8) µmol/L. Conclusion: Supplementation of single dose 400 µg vitamin B12 for 42 consecutive days on adult vegan subjects was proven to elevate the level of serum vitamin B12 and decrease the level of homocysteine.

Abstrak :
Latar belakang: Akne vulgaris (AV) merupakan kelainan kulit akibat peradangan kronik folikel pilosebasea yang sering dijumpai pada remaja dan dewasa muda. Pada pasien perempuan didapatkan prevalensi AV yang lebih tinggi dan dampak psikososial yang lebih berat. Beberapa studi meneliti terdapat hubungan antara peningkatan kadar homosistein dengan derajat keparahan AV, namun peran homosistein dalam patogenesis AV masih belum jelas. Kadar homosistein ditentukan oleh multifaktor sehingga temuan di Indonesia dapat berbeda dibandingkan penelitian terdahulu. Secara fisiologis, kadar homosistein pada perempuan lebih rendah dari laki-laki. Tujuan: Mendapatkan data kadar homosistein plasma pada pasien perempuan dengan AV ringan (AVR), AV sedang (AVS), dan AV berat (AVB) serta mengetahui korelasi kadar homosistein plasma dengan berbagai derajat keparahan AV. Metode: Studi potong lintang dilakukan terhadap 46 subjek penelitian (SP), direkrut secara consecutive sampling, yang terdiagnosis AV berdasarkan kriteria Lehmann pada bulan April-Juni 2019. Setiap SP akan diambil darahnya untuk dilakukan pemeriksaan kadar homosistein plasma dengan metode chemiluminescent microparticle immuno assay (CMIA). Hasil: Pada pasien perempuan dengan AV didapatkan rerata kadar homosistein plasma kelompok AVR, AVS, dan AVB yaitu 7,39 (1,84) μmol/L, 7,14 (1,73) μmol/L, dan 6,95 (1,14) μmol/L. Terdapat korelasi negatif lemah yang tidak bermakna antara kadar homosistein plasma dengan derajat keparahan AV (r=-0,0964, p=0,524). Kesimpulan: Kadar homosistein plasma ditemukan lebih rendah pada kelompok AVS dan AVB. Dari hasil penelitian didapatkan bahwa semakin rendah kadar homosistein plasma, maka semakin berat derajat keparahan AV. ......Background: Acne vulgaris (AV) is a skin disorder caused by chronic inflammation of pilosebaceus that is primarily found in adolescents and young adults. In female patient, there is a higher prevalence of AV and more severe psychosocial impact. Several studies have investigated association between the levels of serum homocysteine and severity of AV, but the role of homocysteine in AV is not clearly understood. Homocysteine levels are thought to be affected by varying factors, so it is assumed that homocysteine levels in Indonesian people will yield a different results. Physiologically, female has a lower homocysteine levels. Objective: This study aims to know the levels of homocysteine plasma in female patients suffering from mild, moderate, and severe AV, also its correlation with the degree of AV severity. Methods: This cross-sectional study included 46 subjects, recruited by consecutive sampling, who have been diagnosed with AV based on Lehmann criteria on April-June 2019. Blood sample will be taken from each subject to measure homocycsteine plasma levels by using chemiluminescent microparticle immuno assay method (CMIA). Results: In female patients, the mean plasma homocycteine levels of mild, moderate, and severe groups were respectively 7,39 (1,84) μmol/L, 7,14 (1,73) μmol/L, and 6,95 (1,14) μmol/L. There was no significant corelation between plasma homocysteine levels and the degree of acne severity (r=-0,0964, p=0,524). Conclusion: Levels of plasma homocysteine was found lower on moderate and severe AV groups. The lower the levels of plasma homocysteine, the more severe the the degree of acne severity.

Abstrak :
Homosistein (Hcy) adalah asam amino yang mengandung tiol dan memiliki potensi sebagai penanda biologis dari komplikasi terkait diabetes melitus tipe 2, seperti makrovaskular dan mikrovaskular. Tujuan dari penelitian ini adalah untuk mengetahui perbandingan homosistein serum pada pasien diabetes melitus tipe 2 dengan terapi metformin dan kombinasi metformin-glimepirid. Penelitian dilakukan dengan desain potong lintang dengan metode consecutive sampling di Puskesmas Kecamatan Pasar Minggu dan Puskesmas Depok Jaya. Sampel darah subjek penelitian dikumpulkan untuk pengukuran HbA1c dan kadar homosistein serum. Kadar homosistein serum diukur menggunakan Axis® Homocysteine EIA Kit. Total 125 partisipan dibagi menjadi dua kelompok yaitu pengguna terapi metformin (n=57) dan kombinasi metformin-glimepirid (n=68). Tidak terdapat perbedaan yang bermakna secara statistik pada karakteristik dasar dan klinis kedua kelompok kecuali regimen terapi (metformin) (p=0,003). Tidak terdapat perbedaan yang bermakna (p=0,163) pada hasil pengukuran kadar homosistein serum pada kelompok metformin (12,03±3,45) dan kombinasi metformin-glimepirid (13,08±4,69). Hiperhomosisteinemia (µmol/L) lebih banyak ditemukan pada kelompok kombinasi metformin-glimepirid dibandingkan dengan kelompok metformin, namun tidak bermakna secara statistik (p=0,113). Terdapat faktor yang dapat memengaruhi kadar homosistein yaitu jenis kelamin, durasi menderita DMT2, rutinitas olahraga, dan regimen terapi (glimepirid). Oleh karena itu dapat disimpulkan bahwa tidak terdapat perbedaan kadar homosistein serum pada kelompok metformin dan kombinasi metformin-glimepirid. ......Homocysteine (Hcy) is an amino acid that contains thiols and has the potential to be a biological marker of complications related to type 2 diabetes mellitus, such as macrovascular and microvascular. The purpose of this study was to determine the comparison of serum homocysteine in type 2 diabetes mellitus patients with metformin and metformin-glimepiride combination therapy. The study was conducted with a cross-sectional design with a consecutive sampling method at the Pasar Minggu Subdistrict Health Center and the Depok Jaya Health Center. Blood samples of the research subjects were collected for measurements of HbA1c and serum homocysteine levels. Serum homocysteine levels were measured using the Axis® Homocysteine EIA Kit. A total of 125 participants were divided into two groups, users of metformin therapy (n=57) and metformin-glimepiride combinations (n=68). There were no statistically significant differences in baseline and clinical characteristics among groups except for therapeutic regimen (metformin) (p=0,003). There was no significant difference (p=0,163) in the measurement results of serum homocysteine levels in the metformin (12,03±3,45) and metformin-glimepiride combination (13,08±4,69). Hyperhomocysteinemia (μmol/L) was more commonly found in the metformin-glimepiride combination group compared to the metformin group, but was no statistically significant difference (p=0.113). Gender, duration of T2DM, regular exercise, and therapeutic regimen (glimepiride) are factors that can affect homocysteine levels. Therefore, it can be said that there was no difference in serum homocysteine levels in the metformin and metformin-glimepiride groups.

Abstrak :
Tujuan : Mengetahui pengaruh suplementasi vitamin B6, B12 dan asam folat terhadap kadar homosistein plasma pada lansia dalam rangka mengurangi risiko terjadinya aterosklerosis. Tempat : Panti werdha Santa Anna - Jakarta. Bahan dan cara : penelitian eksperimental pra dan pasca suplementasi vitamin B6 (10 mg), B12 (400 µg) dan asam folat (1 mg) yang diberikan per oral, sekali sehari selama 6 minggu, terhadap 10 subyek lansia (60 tahun) yang telah memenuhi kriteria penerimaan dan penolakan. Data yang dikumpulkan meliputi data non nutrisi, data nutrisi, data antropametri, status vitamin B6, kadar vitamin B12 serum dan asam folat serum dan kadar homosistein plasma. Hasil : Pada pra suplementasi, diketahui prevalensi subyek dengan hiperhomosisteinemia tipe ringan sebesar 70%. Prevalensi defisiensi vitamin B6 (KA ASATE>I,40), 812 (<258 pmol/L) dan asam folat (<15 nmo/L) adalah 30%,30% dan 90%. Prevalensi defisiensi vitamin B6, B12 dan asam folat pada subyek dengan hiperhomosisteinemia adalah 14%, 43% dan 85%. Pada pasca suplementasi didapatkan perbaikan pada seluruh hasil pemeriksaan laboratorium secara bermakna (p<0,05) yaitu penurunan KA ASATE 11,68%, kenaikan kadar vitamin B12 serum 111,75%, kenaikan kadar asam folat serum 139,05% dan penurunan kadar homosistein plasma 36,68%. Kesimpulan : Suplementasi vitamin B6, B12 dan asam folat terbukti secara efektif dan elision dapat memperbaiki status vitamin dan menurunkan kadar homosistein plasma secara bermakna pada seluruh subyek penelitian.

---

Objective : To identify the effect of vitamin B6, B12 and folate supplementation to plasma homocysteine concentration of elderly people in respect of minimizing atherosclerosis risks. Place :Panri werdha Santa Anna - Jakarta. Materials and Methods :Experimental study of pre and post oral supplementation of vitamin B6 (10 mg), B12 (400 }1g) and folate (1 mg), once a day for 6 weeks continuously applied to 10 elderly subjects NO years) passing through pre-defined inclusion criteria. Relevant information and data was collected through questionnaire, field observation and laboratory measurement which comprise of ages, sex, education, anthropometrics, dietary intake, food frequency amount, food habits, vitamin B6, B12 and folate status and finally plasma homocysteine concentration. Results :During pre-supplementation, 70% of subjects was classified as moderate hyperhomocysteinemia. Cut off points to define deficiency vitamin status are erytrocyte aspartate aminotransferase activity coefficient (EAST-AC) >1,40 for vitamin B6 , serum vitamin B12 and folate concentrations were <258 pmol/L and <15 nmol/L respectively. The overall prevalence of deficiencies vitamin B6, B12 and folate status were 30%, 30% and 90% respectively. The prevalence of deficiencies vitamin B6, B12 and folate status in hyperhomocysteinemia subjects were 14%, 43% and 85% respectively. During post supplementation, no more vitamins deficiencies subjects was detected. Post supplementation laboratory measurement indicate the following significant improvement (p<0,05) on EAST-AC reduction 11,68%, serum vitamin B12 concentration improvement to 111,75%, serum folate concentration improvement to 139,05% and reduction of plasma homocysteine concentration of 36,68%. Conclusion :Supplementation of vitamin B6, B12 and folate are effectively and significantly improve both vitamin status and plasma homocysteine concentration level of all subjects.

Abstrak :
Latar Belakang : Peningkatan kadar homosistein merupakan faktor risiko terjadinya aterosklerosis dan trombogenesis. Baik faktor genetik maupun lingkungan mempunyai pengaruh terhadap kadar plasma homosistein. Pada penelitian ini, kami meneliti gambaran dari homosistein pada populasi PJK di Jakarta dan Malang serta hubungannya dengan enzim MTHFR, Vit. B6, Vil. B12, dan asam folat. Metode dan Hasil : Penelitian deskriptif ini melibatkan 30 pasien PJK di Jakarta dan 12 pasien di Malang. Subyek yang direkrut di Malang lebih muda, tetapi tidak ada perbedaan dalam jenis kelamin, 1MT, diabetes, dan merokok. Tidak ada perbedaan pada profil lipid diantara dua populasi. Subyek di Malang mempunyai kadar homosistein lebih tinggi (median 18 mmol/dL vs 9,1 mmol/dL; p < 0,001), kadar MTHFR yang lebih rendah (median 0,105 IU vs 0,157 IU; p = 0,019) kadar asam folat yang lebih rendah (median 7,1 vs 11,2 ng/mL; p = 0,005), kadar vit. B12 yang lebih rendah (median 273 ng/mL vs 429,5 ng/mL; p = 0,032). Tidak ada perbedaan pada kadar vit B6. Analisis dari hubungan menunjukkan hubungan yang terbalik antara homosistein dan pit. B12 (r = - 0,43, p = 0,004) dan asam folat (r = -0,39,p = 0,01). Kesimpulan : Tidak ada perbedaan kadar homosistein, MTHFR, asam folat, dan vitamin B12 pada populasi PJK (Jakarta dan Malang). Terdapat hubungan yang terbalik antara homosistein dan vit. B12 serta asam folat.

---

Background : Increased homocysleine level is a risk factor for atherosclerosis and thrombogenesis_ Both genetic and environmental factors influence plasma level of homocysteine. In this study, we examine the distribution of homocysteine in population of CAD in Malang and Jakarta and the association between homocysteine, enzyme MTHFR, Vit. B6, Vii. B12, and folic acid. Methods and Results : This is a descriptive study including 30 CAD patients in Jakarta and 12 in Malang. Subjects recruited in Malang is younger, but no difference in gender, BMI, smoking and diabetes. No difference in lipid profile between both populations. Subjects in Malang have higher level of homocysteine (median 18 mmol/dL vs 9.1 mmol/dL; p <0.001), lower level of MTHFR (median 0.105 IU vs 0.157 IU; p = 0.019), lower level of Folic acid (median 7.1 vs 11.2 ng/mL; p = 0.005), lower level of Vit. B12 (median 273 ng/mL vs 429.5 ng/mL; p = 0.032). There is no difference in level of Vit. B6. Analysis of association showed inverse relationship between homocysteine and vit B12(r - -0.43, p = 0.004) and folic acid (r = -0.39,p =0.01). Conclusion : There is difference in level of homocysteine, MTHFR, folic acid and vii. B12 between populations coronary artery disease ( Jakarta and Malang). There is inverse relationship between homocysteine and vit B12 and folic acid.