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"ABSTRAKPemeriksaan IMLTD merupakan pengolahan darah untuk memastikan darah yang diberikan telah aman. Darah reaktif harus diperiksa ulang dengan menggunakan reagen yang sama dan in duplicate. Jika hasil RR maka darah harus dimusnahkan. Donor diberitahukan untuk tidak menyumbangkan darah dan melakukan uji diagnostik di RS. Sering terjadi perbedaan hasil antara uji saring UTD dengan uji diagnostik. Konfirmasi diperlukan pada kasus dimana terjadi perbedaan hasil. Western Blot (WB) adalah uji konfirmasi untuk mendeteksi antibodi terhadap virus. Saat ini juga terdapat metode immunokromatografi yang memiliki spesifisitas sama dengan WB. Tujuan penelitian mengetahui uji konfirmasi metoda imunokromatografi menjamin keamanan darah terhadap HIV. Desain penelitian deskriptif analitik dan uji diagnostik dengan 77 sampel yang memenuhi nilai inklusi. sampel berupa darah lengkap dengan volume tiga ml sebanyak 6 tabung. Hasil menunjukkan perbandingan WB dengan immunokromatografi didapatkan 5 sampel reaktif WB maupun immunokromatografi, 5 sampel non reaktif WB dan reaktif immunokromatografi. 67 sampel non reaktif WB maupun immunokromatografi. Kesimpulan terdapat perbedaan hasil reaktif dari metode ChLIA dengan hasil pemeriksaan diagnostik menggunakan RDT, WB dan imunokromatografi dan diferensiasi Ab HIV 1 dan 2 dan ketepatan konfirmasi Imunokromatografi memiliki kesesuaian hasil HIV 1 dengan WB.

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ABSTRACTIMLTD examination is a blood treatment to ensure that the blood given is safe. Reactive blood must be re-examined using the same reagent and in duplicate. If the RR results, the blood must be destroyed. Donors were told not to donate blood and carry out diagnostic tests at the hospital. There are often differences in the results between blood centers test and the diagnostic test. Confirmation is needed in cases where there are differences in results. Western Blot (WB) is a confirmation test for detecting antibodies to the virus. At present there are also immunochromatographic methods that have the same specificity as WB. The aim of the study was to determine the confirmation test of the immunochromatographic method to ensure blood safety against HIV Descriptive analytic research design and diagnostic test with 77 samples that meet the inclusion value. samples in the form of complete blood with a volume of three ml as many as 6 tubes. The results showed a comparison of immunocromatographic WB with 5 reactive WB samples as well as immunochromatography, 5 non-reactive WB samples and immunochromatographic reactive. 67 WB non-reactive samples and immunochromatography. Conclusion there are differences in the reactive results of the ChLIA method with the results of diagnostic examinations using RDT, WB and immunochromatography and differentiation of Ab HIV 1 and 2 and the accuracy of confirmatory immunochromatography that matches HIV 1 results with WB."

"Latar belakang: Aspergilosis paru kronik (APK) merupakan komplikasi yang sering menyebabkan munculnya sekuela respiratori pada pasien bekas tuberkulosis (TB) paru. Diagnosis APK dapat dilakukan dengan pemeriksaan serologi IgG spesifik Aspergillus. Metode tersebut memerlukan waktu tertentu, sumber daya, dan fasilitas khusus, sehingga sulit diterapkan di fasilitas kesehatan dengan sumber daya terbatas. Metode baru Immunocromatography Test (ICT) LD Bio Aspergillus dilaporkan lebih mudah digunakan, cepat dan murah; tetapi akurasi diagnostiknya belum diketahui di Indonesia. Tujuan: Penelitian ini bertujuan untuk mengetahui akurasi diagnostik LD Bio ICT Aspergillus dibandingkan dengan IgG spesifik Aspergillus pada pasien bekas TB. Metode: Penelitian dengan desain potong lintang ini dilakukan dari April 2019 – Oktober 2020. Perekrutan subjek dilakukan di RSUP Persahabatan dan prosedur pemeriksaan mikologi dilakukan di Laboratorium Parasitologi FKUI. Serum pasien bekas TB diperiksa menggunakan LD Bio ICT Aspergillus dan IgG spesifik Aspergillus Dynamiker ELISA. Hasil kedua pemeriksaan dibandingkan untuk melihat akurasi diagnosis LD Bio ICT. Hasil: Dari 82 pasien yang sesuai dengan kriteria inklusi, terdapat 57 pasien (69,5%) laki-laki, rerata usia pasien 51,27±12,55 tahun. Median IMT 18,67 (10,38-31,18). Sebanyak 40 pasien (48,7%) menunjukkan hasil positif IgG spesifik Aspergillus. Adapun hasil positif LD Bio ICT Aspergillus didapatkan pada 35 pasien (42,7%). Sensitivitas dan spesifisitas LD Bio ICT dibandingkan dengan pemeriksaan IgG spesifik Aspergillus adalah 50,0% dan 64,3%, sedangkan nilai duga positif dan negatifnya adalah 57,1% dan 57,5%. Simpulan: LD Bio ICT dapat digunakan untuk mendiagnosis APK pada pasien bekas TB Paru di fasilitas kesehatan dengan sumber daya terbatas.

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Background: Chronic pulmonary aspergillosis (CPA) is a common complication following prior pulmonary tuberculosis (TB) causing respiratory sequelae. Although CPA may lead to worse prognosis, it is still underdiagnosed. Serology test such as Aspergillus-specific IgG is the recommended test for CPA diagnosis. However, this diagnostic procedure is time-consuming, require a lot of resources and certain skills, making this procedure not always easy to implement in limited facilities. The LDBio Diagnostic introduced a novel, affordable, and easy to use serology test, LD Bio Immunocromatography Test (ICT). Nevertheless, LD Bio ICT’s diagnostic accuracy in Indonesia is still unknown.

Study aims: This study aimed to determine the diagnostic accuracy of LD Bio ICT with Aspergillus-specific IgG as comparison in previous pulmonary TB patients.

Methods: This cross-sectional study was conducted in April 2019 – October 2020. Subject recrutment was done in National Referral Centre Persahabatan Hospital and serological test was conducted in the Parasitology Laboratory, Faculty of Medicine Universitas Indonesia. Eighty two sera of previous pulmonary TB patients were serologically tested using LD Bio ICT Aspergillus (France) and Aspergillus-specific IgG was tested using Dynamiker ELISA kit. Results of both tests were then compared to determine the diagnostic accuracy of LD Bio ICT.

Results: Of 82 patients met the inclusion criteria, 57 patients (69.5%) were men, the mean age was 51.27±12.55 years old. The BMI median was 18.67 (10.38-31.18). Forty patients (48.7%) showed positive Aspergillus-specific IgG Dynamiker results. Meanwhile, 35 patients (42.7%) showed positive results of LD Bio ICT Aspergillus. Compared to this finding, LD Bio ICT sensitivity and specificity were 50.0% and 64.3% respectively. In addition, the positive and negative prediction value of LD Bio ICT in this study were 57.1% and 57.5%.

Summary: LD Bio ICT is useful for the diagnosis of CPA in previous pulmonary TB patients in resource-limited settings."