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Abstrak :
ABSTRACT
Telah dilakukan penelitian untuk mengetahui pengaruh penambahan asam amino sistein dan agen pengkelat EDTA dalam larutan Tris-buffer terhadap integritas DNA dan morfometri kepala spermatozoa sapi friesian holstein (Bos taurus) pascapengeringbekuan. Semen dikoleksi seminggu sekali selama enam minggu. Sampel semen sapi diencerkan dengan larutan Tris-buffer yang ditambahkan dengan asam maino sistein dan EDTA. Kelompok perlakuan terbagi menjadi empat, yaitu kelompok larutan pengencer Tris-buffer tanpa ditambahkan asam amino sistein atau EDTA (KK), larutan pengencer Tris-buffer ditambahkan asam amino sistein (KP1), larutan pengencer Tris-buffer ditambahkan dengan EDTA (KP2), larutan pengencer Tris-buffer ditambahkan asam amino sistein dan EDTA (KP3). Hasil integritas DNA spermatozoa sapi friesian holstein pascapengeringbekuan pada semua kelompok perlakuan 100% stabil dan tidak mengalami kerusakan. Hasil analisis varians (ANAVA) menunjukkan bahwa pemberian asam amino sistein 10 mM dan EDTA 50 mM terhadap panjang dan area morfometri kepala spermatozoa sapi friesian holstein (Bos taurus) pascapengeringbekuan tidak berbeda nyata antar kelompok (P>0,05), sedangkan terhadap lebar morfometri kepala spermatozoa sapi friesian holstein pascapengeringbekuan berbeda nyata antar kelompok (P<0,05).

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ABSTRACT
The research was conducted to assess the effect of cysteine and EDTA chelating agent on DNA integrity and head morphometry of friesian holstein (Bos taurus) cattle spermatozoa after freeze-drying. Semen was collected every once a week for six weeks. The control group, semen diluted in Tris-buffer solution without cysteine or EDTA chelating agent, while in treatment groups semen diluted in Tris-buffer solution with addition of 10 mM cysteine, addition of 50 mM EDTA chelating agent, then the last group with addition of 10 mM cysteine and 50 mM EDTA chelating agent.  Based on the result, DNA integrity of freeze dried spermatozoa, showed that 100% DNA stablized in control and all groups. Result of variances (ANAVA) one factor test, showed that addition of cysteine 10 mM and EDTA chelating agent 50 mM were not significantly different in length and area morphometry  between groups parameter (P > 0,05), while addition of 10 mM cysteine and 50 mM EDTA chelating agent were significantly different in width morphometry between  groups parameter (P < 0,05).

Abstrak :
Latar Belakang: Infertilitas adalah ketidakmampuan pasangan untuk hamil setelah 1 tahun berhubungan seksual secara teratur tanpa kontrasepsi. Infertilitas merupakan masalah kesehatan reproduksi yang cukup marak terjadi dan sekitar 50 dari kasus infertilitas disebabkan oleh faktor laki-laki saja atau gabungan antara faktor laki-laki dan perempuan. Buruknya vitalitas dan integritas DNA spermatozoa merupakan faktor yang berhubungan dengan infertilitas pada laki-laki. Tujuan: Mengetahui korelasi antara vitalitas dengan integritas DNA spermatozoa pada laki-laki infertil di Klinik Yasmin RSCM Kencana. Metode: Jenis desain penelitian yang digunakan adalah potong lintang. Subjek penelitian adalah 96 laki-laki infertil. Data diambil dari rekam medis pasien Klinik Yasmin RSCM Kencana, Jakarta. Pemeriksaan vitalitas spermatozoa menggunakan pewarnaan eosin, sedangkan pemeriksaan integritas DNA spermatozoa menggunakan uji sperm chromatin dispersion SCD . Data variabel vitalitas dan integritas DNA spermatozoa pertama-tama diuji normalitas sebarannya, kemudian dianalisis dengan uji korelasi Spearman. Hasil: Penelitian menunjukkan terdapat korelasi negatif yang signifikan dengan kekuatan korelasi sedang antara vitalitas spermatozoa dengan indeks fragmentasi DNA spermatozoa p=0,000, r=-0,505. Kesimpulan: Semakin tinggi vitalitas spermatozoa maka semakin rendah indeks fragmentasi DNA spermatozoa yang artinya semakin baik integritas DNA spermatozoa. Adanya korelasi dengan kekuatan korelasi sedang menunjukkan bahwa vitalitas spermatozoa tidak dapat dipergunakan sebagai prediktor integritas DNA spermatozoa, sehingga diperlukan uji fragmentasi DNA spermatozoa selain uji vitalitas spermatozoa untuk mengevaluasi infertilitas laki-laki. ......Background: Infertility is the inability of sexually active couple to conceive after one year of unprotected sexual intercourse in a reasonable frequency. Infertility is a common reproductive health problem and approximately 50 of infertility cases are caused by factors from male only or a combination of male and female. Both poor sperm vitality and DNA integrity are associated with male infertility. Objective: To analyze the correlation between sperm vitality and DNA integrity in infertile men at Yasmin Clinic RSCM Kencana. Methods: This study was performed with cross sectional method. The subjects of research were 96 infertile men. Data were obtained from medical record at Yasmin Clinic, Cipto Mangunkusumo General Hospital, Jakarta. The method used for sperm vitality assessment was eosin staining, while the method used for sperm DNA integrity assessment was sperm chromatin dispersion test SCD. Sperm vitality and DNA integrity data were tested for normality, and then analyzed by Spearman correlation test. Results: There was statistically significant moderate negative correlation between sperm vitality and DNA fragmentation index p 0,000, r 0,505. Conclusion: Higher value of sperm vitality correlates with lower value of DNA fragmentation index which means that the better the sperm DNA integrity. This moderate negative correlation indicates that sperm vitality cannot be used as a predictor of sperm DNA integrity, therefore in addition to conventional semen analysis and sperm vitality test, sperm DNA fragmentation is also needed to evaluate male infertility.

Abstrak :
ABSTRAK
Penelitian telah dilakukan untuk mengetahui pengaruh penambahan asam amino glutamin dan agen pengkelat EDTA (asam etilen diamin tetraasetat) dalam larutan Tris-buffer pada integritas DNA dan morfometri kepala spermatozoa sapi Friesian Holstein (Boss taurus) pascabeku. Semen dikumpulkan seminggu sekali untuk enam minggu. Sampel semen diencerkan dengan pengencer tris-buffer dan ditambah asam amino glutamin dan EDTA. Kelompok perlakuan dibagi menjadi empat grup hanya berisi larutan penyangga Tris (KK), grup larutan penyangga Tris dengan penambahan EDTA (KP1), kelompok larutan penyangga Tris dengan penambahan asam amino glutamin (KP2), dan kelompok larutan penyangga Tris dengan penambahan EDTA dan asam amino glutamin (KP3). Hasil integritas DNA spermatozoa sapi Friesian Holstein (Bos taurus) setelah pengeringan beku pada semua perlakuan stabil 100% dan tidak rusak. Hasil analisis varians (ANAVA) menunjukkan bahwa pemberian asam amino glutamin dan EDTA pada morfometri kepala spermatozoa Sapi Friesian Holstein (Bos taurus) pasca pengeringan beku pada semua perlakuan antara kedua kelompok tidak menunjukkan perbedaan yang signifikan (P > 0,05). Hasil analisis membran plasma utuh sperma menunjukkan penambahan kombinasi asam amino amino glutamin dan EDTA memiliki efek signifikan dalam menjaga membran membrane plasma (P < 0,05).
ABSTRACT
This study was conducted to determine the effect of adding the amino acid glutamine and the chelating agent EDTA (ethylene diamine tetraacetic acid) in Tris-buffer solution on DNA integrity and sperm head morphometry of Friesian Holstein cattle (Boss taurus) post-frozen. Semen is collected once a week for six weeks. The semen sample was diluted with tris-buffer diluent and the amino acids glutamine and EDTA were added. The treatment group was divided into four groups containing only Tris buffer solution (KK), Tris buffer solution group with the addition of EDTA (KP1), Tris buffer solution group with the addition of amino acid glutamine (KP2), and Tris buffer solution group with the addition of EDTA and amino acids. glutamine (KP3). Friesian bovine spermatozoa DNA integrity results Holstein (Bos taurus) after freeze drying in all treatments was 100% stable and undamaged. The results of the analysis of variance (ANOVA) showed that the administration of amino acids glutamine and EDTA to the spermatozoa head morphometry of Holstein Friesian Cattle (Bos taurus) after freeze drying in all treatments between the two groups did not show a significant difference (P > 0.05). Analysis result intact plasma membrane of sperm showed that the addition of the amino acid combination of glutamine and EDTA had a significant effect in maintaining the plasma membrane (P < 0.05).

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ABSTRAK
Penelitian telah dilakukan untuk mengetahui pengaruh pemberian berbagai konsentrasi glisin terhadap kualitas spermatozoa sapi sumba ongole Bos indicus SO . Seekor sapi SO dijadikan sebagai donor semen. Semen dikoleksi setiap satu minggu sekali selama enam minggu untuk memenuhi pengulangan yang dibutuhkan. Sampel semen sapi SO dibagi menjadi empat kelompok, yaitu: Kelompok kontrol KK dimana semen diencerkan dalam tris kuning telur TKT dan kelompok perlakuan dimana semen diencerkan dalam TKT dengan penambahan glisin konsentrasi 5 mM, 15 mM, dan 25 mM KP1, KP2, dan KP3 . Semen yang telah diencerkan diekuilibrasi dan dibekukan dengan nitrogen cair. Parameter kualitas spermatozoa yang dievaluasi meliputi motilitas, viabilitas, membran plasma utuh MPU , dan integritas DNA. Hasil uji analisis variansi ANAVA satu faktor menunjukkan bahwa nilai rata-rata persentase motilitas, viabilitas, dan MPU spermatozoa sapi SO pascakriopreservasi berbeda nyata.

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ABSTRAK
The present study was conducted to assess the effect of glycine in various concentration on spermatozoa quality of sumba ongole Bos indicus SO cattle postcryopreservation. One SO cattle was used as semen donor. The semen of SO cattle was collected once a week for six weeks to fulfill the required repetition. The semen sample was divided into four groups, consisting of control group KK which is semen diluted in tris citrate fructose egg yolk TCFY extender and treatment group which is semen diluted in TCFY with glycine additives at concentration 5 mM, 15 mM, and 25 mM KP1, KP2, and KP3 . Diluted semen was equilibrated and freezed in liquid nitrogen. Parameters of spermatozoa quality include percentage of motility, viability, membrane integrity, and DNA integrity were assessed. One factor analysis of variance ANOVA test showed that average value of motility, viability, and membrane integrity of postcryopreservation spermatozoa were significantly differed between glycine additives group as compared to control group P 0,05. The DNA integrity of postcryopreservation spermatozoa were stable in treatment group.

Abstrak :
ABSTRAK
Telah dilakukan penelitian untuk mengetahui pengaruh pemberian berbagai konsentrasi asam amino sistein 3 mM, 5 mM, dan 7 mM terhadap kualitas spermatozoa sapi sumba ongole Bos indicus pascakriopreservasi. Seekor sapi sumba ongole SO dijadikan sebagai donor semen. Semen dikoleksi setiap satu minggu sekali selama enam minggu untuk memenuhi pengulangan yang dibutuhkan. Sampel semen sapi SO diencerkan menggunakan pengencer Tric Citrite Fructose Yolk TCFY dan penambahan sistein. Kelompok kontrol 0 mM , semen diencerkan dalam TCFY tanpa penambahan asam amino, sedangkan pada kelompok perlakuan semen diencerkan dalam TCFY dengan penambahan sistein sebesar 3 mM; 5 mM; dan 7 mM. Semen yang telah diencerkan diekuilibrasi dan dibekukan dalam nitrogen cair. Parameter kualitas spermatozoa yang dievaluasi meliputi motilitas, viabilitas, membran plasma utuh MPU , dan integritas DNA. Berdasarkan hasil penelitian, terjadi peningkatan persentase motilitas, viabilitas, dan MPU pada kelompok perlakuan berbagai konsentrasi sistein 3 mM; 5 mM; dan 7 mM jika dibandingkan dengan 0 mM. Hasil uji ANAVA satu faktor menunjukkan pemberian berbagai konsentrasi asam amino sistein memiliki nilai rata-rata persentase motilitas, viabilitas, dan MPU yang berbeda nyata.

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ABSTRACT
The research was conducted to assess the effect of cysteine in various concentration 3 mM, 5 mM, and 7 mM on spermatozoa quality of sumba ongole Bos indicus cattle postcryopreservation. Sumba ongole SO cattle serve as donors of semen. Semen was collected every once a week for six weeks to meet the repetition needed. The semen samples were diluted in Tric Citrite Fructose Yolk TCFY extender and the addition of cysteine. The control group 0 mM semen diluted in TCFY without cysteine, while in the treatment group, semen diluted in TCFY with the addition of cysteine 3 mM 5 mM and 7 mM. Semen has been diluted equilibrated and frozen in liquid nitrogen. The parameters of quality of spermatozoa are evaluated include motility, viability, membrane plasma integrity and DNA integrity. Based on the result, the increase in the percentage of motility, viability, and membrane plasma integrity compared to just added extender TCFY 0 mM . The one factor ANOVA showed that various concentrations of cysteine were significantly different between treatment group and control group.

Abstrak :
ABSTRAK
Penelitian telah dilakukan untuk mengetahui pengaruh pemberian berbagai konsentrasi glisin terhadap kualitas spermatozoa sapi sumba ongole Bos indicus SO . Seekor sapi SO dijadikan sebagai donor semen. Semen dikoleksi setiap satu minggu sekali selama enam minggu untuk memenuhi pengulangan yang dibutuhkan. Sampel semen sapi SO dibagi menjadi empat kelompok, yaitu: Kelompok kontrol KK dimana semen diencerkan dalam tris kuning telur TKT dan kelompok perlakuan dimana semen diencerkan dalam TKT dengan penambahan glisin konsentrasi 5 mM, 15 mM, dan 25 mM KP1, KP2, dan KP3 . Semen yang telah diencerkan diekuilibrasi dan dibekukan dengan nitrogen cair. Parameter kualitas spermatozoa yang dievaluasi meliputi motilitas, viabilitas, membran plasma utuh MPU , dan integritas DNA. Hasil uji analisis variansi ANAVA satu faktor menunjukkan bahwa nilai rata-rata persentase motilitas, viabilitas, dan MPU spermatozoa sapi SO pascakriopreservasi berbeda nyata P

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ABSTRACT
The present study was conducted to assess the effect of glycine in various concentration on spermatozoa quality of sumba ongole Bos indicus SO cattle postcryopreservation. One SO cattle was used as semen donor. The semen of SO cattle was collected once a week for six weeks to fulfill the required repetition. The semen sample was divided into four groups, consisting of control group KK which is semen diluted in tris citrate fructose egg yolk TCFY extender and treatment group which is semen diluted in TCFY with glycine additives at concentration 5 mM, 15 mM, and 25 mM KP1, KP2, and KP3 . Diluted semen was equilibrated and freezed in liquid nitrogen. Parameters of spermatozoa quality include percentage of motility, viability, membrane integrity, and DNA integrity were assessed. One factor analysis of variance ANOVA test showed that average value of motility, viability, and membrane integrity of postcryopreservation spermatozoa were significantly differed between glycine additives group as compared to control group P 0,05 . The DNA integrity of postcryopreservation spermatozoa were stable in treatment group.

Abstrak :
ABSTRAK
Telah dilakukan penelitian untuk mengetahui pengaruh pemberian berbagai konsentrasi asam amino glutamin 5 mM, 15 mM, dan 25 mM terhadap kualitas spermatozoa sapi sumba ongole Bos indicus pascakriopreservasi. Semen diperoleh dari satu ekor sapi SO yang dikoleksi setiap satu minggu sekali selama enam kali pengulangan. Sampel semen diencerkan menggunakan pengencer tris kuning telur TKT dan penambahan glutamin. Kelompok kontrol KK , semen diencerkan dalam TKT tanpa penambahan asam amino, sedangkan pada kelompok perlakuan semen diencerkan dalam TKT dengan penambahan glutamin sebesar 5mM; 15mM; dan 25mM KP1, KP2, dan KP3 . Semen yang telah diencerkan kemudian diekuilibrasi selama 2 jam dan masuk ke tahap pembekuan menggunakan nitrogen cair. Parameter kualitas spermatozoa meliputi presentase motilitas, viabilitas, integritas membran plasma utuh, dan integritas DNA. Hasil uji analisis varians ANAVA satu faktor menunjukkan bahwa nilai rata-rata persentase motilitas, viabilitas, dan MPU spermatozoa sapi SO pascakriopreservasi berbeda nyata antara kelompok perlakuan dengan kelompok kontrol P

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ABSTRACT
The research was conducted to assess the effect of glutamine in various concentration 5mM, 15 mM, and 25 mM on spermatozoa quality of sumba ongole cattle Bos indicus postcryopreservation. Semen was obtained from one SO cattle and was collected once a week for six repetitions. The semen samples were diluted in tris citric frutose egg yolk TCFY extender and the addition of glutamine. The control group KK semen diluted in TCFY without glutamine, while in the treatment group, semen diluted in TCFY with the addition of glutamine 5mM KP1 15mM KP2 and 25 mM KP3 . Diluted semen was equilibrated for 2 hours and enters the freezing stage using liquid nitrogen. Parameters of spermatozoa quality include precentage of motility, viability, membran integrity, and DNA integrity. One factor analysis of variance ANOVA test showed that the mean value of motility, viability, and membrane integrity of SO cattle spermatozoa postcryopreservation were significantly different between treatment group and control group.

Abstrak :
ABSTRAK
Penelitian telah dilakukan untuk mengetahui pengaruh penambahan ethylenediamine tetraacetic acid EDTA dengan konsentrasi sebesar 0,5 mM, 1 mM, dan 1,5mM terhadap kualitas spermatozoa sapi peranakan ongole PO pascapengeringbekuan.Semen diperoleh dari dua ekor sapi PO, dikoleksi satu minggu sekali untuk masingmasingsapi PO selama tiga minggu untuk memenuhi pengulangan yang dibutuhkan.Sampel semen diencerkan ke dalam medium pengencer Tris kuning telur TKT 20. Sampel semen dibagi ke dalam empat kelompok meliputi kelompok kontrol KK dankelompok perlakuan KP1, KP2, dan KP3. Kelompok kontrol KK, semen diencerkanke dalam medium TKT 20 tanpa penambahan EDTA. Kelompok perlakuan, semendiencerkan ke dalam medium TKT 20 dengan penambahan 0,5 mM KP1, 1 mM KP2, dan 1,5 mM EDTA KP3. Semen yang telah diencerkan diekuilibrasi pada suhu5 C selama tiga jam, dibekukan dengan nitrogen cair, dikeringbekukan menggunakanmesin freeze-dryer dengan suhu -60 C dan tekanan 0,011 mBar selama 24 jam, dandisimpan pada suhu 5 C selama dua hari. Parameter kualitas spermatozoa sapi PO yangdievaluasi meliputi persentase viabilitas, integritas membran, abnormalitas, danintegritas DNA. Hasil uji analisis variansi ANAVA satu faktor P < 0,05 menunjukkan bahwa nilai rata-rata persentase integritas membran spermatozoa sapi POpascapengeringbekuan berbeda nyata. Hasil uji perbandingan berganda Tukeymenunjukkan bahwa terdapat perbedaan yang nyata antarkelompok perlakuan KKdengan KP2 terhadap persentase integritas membran spermatozoa sapi POpascapengeringbekuan. Hasil evaluasi integritas DNA spermatozoa PO menunjukkanbahwa kelompok perlakuan KP1, KP2, dan KP3 memiliki kecenderungan lebih baikdalam mempertahankan integritas DNA pascapengeringbekuan dibandingkan dengankelompok kontrol KK.

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ABSTRACT
The research was conducted to assess the effect of Ethylene Diamine Tetraacetic Acid EDTA in various concentration 0,5 mM, 1 mM, and 1,5 mM on post freeze dryingspermatozoa quality of ongole crossbreed cattle. The semen was collected from twoongole crossbreed cattle, once a week for each ongole crossbreed cattle. The semensamples were diluted in Tris egg yolk extender. The semen samples were divided intofour groups consist of the control group KK and the treatment groups KP1, KP2, andKP3. Control groups KK semen diluted in 20 Tris egg yolk extender withoutEDTA. Treatment groups semen diluted in 20 Tris egg yolk extender with 0,5 mM KP1, 1 mM KP2, and 1,5 mM EDTA KP3. The diluted semen samples wereequilibrated at 5 C for three hours, frozen in liquid nitrogen, freeze dried in the freezedryermachine with 60 C and 0,011 mBar for 24 hours, then stored in 5 C for twodays. Parameters of spermatozoa quality include the percentage of viability, membraneintegrity, abnormality, and DNA integrity. One factor analysis of variance ANOVA test P 0.05 showed that the mean value of membrane integrity of ongole crossbreedcattles post freeze drying spermatozoa was significantly different. Tukis honestsignificance test P 0.05 showed that significant differences between KK along withKP2 on the percentage of membrane integrity of ongole crossbreed cattles post freezedryingspermatozoa. The result of evaluated DNA integrity showed that the treatmentgroups KP1, KP2, and KP3 were better at maintaining DNA integrity of ongolecrossbreed cattles post freeze drying spermatozoa than the control group KK.