Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
Penelitian bertujuan untuk memperoleh informasi keanekaragaman khamir dari bunga Jatropha integerrima Jacq. di kampus Universitas Indonesia, Depok. Khamir diisolasi dari 30 bunga (15 bunga jantan dan 15 bunga betina). Hasil elektroforesis menunjukkan terdapat polimorfisme panjang (300--700 pb) daerah ITS rDNA 26 isolat khamir representatif yang mengindikasikan adanya keragaman genetik di antara isolat tersebut. Berdasarkan data sequence, 26 isolat khamir representatif tersebut secara taksonomi beragam, yaitu terdiri dari 13 species yang berasal dari phylum Ascomycota (class Hemiascomycetes (Candida)) dan dari phylum Basidiomycota (class Urediniomycetes (Sporidiobolus), Ustilaginomycetes (Pseudozyma & Ustilago), dan Hymenomycetes (Bullera & Cryptococcus)).

Abstrak :
Penelitian ini bertujuan untuk mengidentifikasi 15 strain kapang dari lima manuskrip kuno berbahan kertas daluang asal perpustakaan Fakultas Ilmu Pengetahuan Budaya Universitas Indonesia (FIB UI) dan melakukan deskripsi morfologi kapang-kapang tersebut. Identifikasi dilakukan berdasarkan analisis sekuens daerah Internal Transcribed Spacers (ITS) rDNA dan pengamatan morfologi kapang dilakukan pada Czapek’s Dox Agar (CDA). Primer forward ITS1 dan primer reverse ITS4 digunakan untuk amplifikasi daerah ITS rDNA. Hasil elektroforesis produk PCR menunjukkan panjang daerah ITS dari 15 strain kapang tersebut bervariasi antara 500 pb--900 pb. Sebelas strain kapang memiliki homologi ITS rDNA dengan spesies terdekat Aspergillus clavatus Desm. (1 strain), Aspergillus flavus group (1 strain), Aspergillus niger van Tieghem (1 strain), Penicillium citrinum Thom (6 strain), Penicillium janthinellum Biourge (1 strain), dan Penicillium oxalicum Currie & Thom (1 strain) dan termasuk anggota ordo Eurotiales, kelas Plectomycetes, dari filum Ascomycota. Satu strain memiliki homologi ITS rDNA dengan spesies terdekat Pseudocercospora sp. (1 strain) dan termasuk anggota ordo Capnodiales, kelas Dotthideomycetes, dari filum Ascomycota. Tiga strain kapang (Penicillium sp. FIB.PRI.6.1, Fraseriella sp. FIB.PRI.6.2, dan mycelia sterilia FIB.PRII.3) belum berhasil diidentifikasi hingga tingkat spesies. ......The aims of this research were to identify 15 mould strains from five old manuscripts of daluang paper from the library of Fakultas Ilmu Pengetahuan Budaya Universitas Indonesia (FIB UI) and to describe their morphology. Identification was carried out based on analysis of Internal Transcribed Spacers (ITS) region of rDNA sequence. Observation of the mould’s morphology was carried out on Czapek’s Dox Agar (CDA). Forward primer ITS1 and reverse primer ITS4 were used to amplify the ITS region of rDNA. Gel electrophoresis results showed that the lengths of ITS region from 15 mould strains were on the range of 500 bp--900 bp. Eleven strains showed ITS rDNA sequence similarities to Aspergillus clavatus Desm. (1 strain), Aspergillus flavus group (1 strain), Aspergillus niger van Tieghem (1 strain), Penicillium citrinum Thom (6 strains), Penicillium janthinellum Biourge (1 strain), Penicillium oxalicum Currie & Thom (1 strain). The strains belong to order Eurotiales, Class Plectomycetes, phylum Ascomycota. One strain showed ITS rDNA sequence similarity to Pseudocercospora sp. (1 strain). The strain belongs to order Capnodiales, class Dotthideomycetes, phylum Ascomycota. Three strains (Penicillium sp. FIB.PRI.6.1, Fraseriella sp. FIB.PRI.6.2, and mycelia sterilia FIB.PRII.3) were unable to be identified to species level.

Abstrak :
Recently the problem of garbage in Indonesia metropoliss become important attention,because effect of which is generated from management of bad garbage can generate various accident like floods stuffed up aqueducts by thrown garbage to river and aqueducts,various disease able to be generated by happing of garbage in any place....

Abstrak :
An effective and real-time traffic information network is highly important and could contribute to decreasing traffic volume and costs by reducing fuel consumption and saving time for drivers in reaching their destinations. This study provides an extensive analysis regarding the drivers? acceptance levels of the current implementation of the Intelligent Transport System (ITS) in Kuala Lumpur. A proposed model from the literature review based on the known Technology Acceptance Model (TAM) is introduced. The ITS system characteristics, information quality, system quality, and service quality were investigated as external variables. The resulting analysis showed that information quality is the highest influential factor followed by system quality. The results also revealed that service quality had no effect on acceptance levels.

Abstrak :
This paper introduces a holistic view to intelligent transport systems (ITS) by providing four different perspectives: the technological, economic, system performance and market views. These perspectives are essential for a thorough understanding of ITS systems, which extend beyond traditional transportation engineering. ITS require management tools related to systems engineering (i.e. architectures); unique features are related to transport economics, and they differ from traditional transport engineering solutions. ITS technologies can improve transport system performance in numerous ways; furthermore, ITS is an important business segment for many technology companies and an industry by its own right. Different types of expertise and management are required for this meta-system, which is comparable to the entire transport system in terms of organisational objectives.

Abstrak :
An effective and real-time traffic information network is highly important and could contribute to decreasing traffic volume and costs by reducing fuel consumption and saving time for drivers in reaching their destinations. This study provides an extensive analysis regarding the drivers’ acceptance levels of the current implementation of the Intelligent Transport System (ITS) in Kuala Lumpur. A proposed model from the literature review based on the known Technology Acceptance Model (TAM) is introduced. The ITS system characteristics, information quality, system quality, and service quality were investigated as external variables. The resulting analysis showed that information quality is the highest influential factor followed by system quality. The results also revealed that service quality had no effect on acceptance levels.

Abstrak :
Penelitian ini bertujuan untuk mengidentifikasi kapang dari dua manuskrip Cina lama yang mengalami deteriorasi asal plot 1 Ruang Naskah PP-UI Depok berdasarkan data sekuens daerah internal transcribed spacers ribosomal DNA ITS rDNA . Pengambilan sampel kapang dari manuskrip dengan metode swab dan isolasi kapang dengan metode culture-dependent. Amplifikasi daerah ITS rDNA dan DNA sequencing menggunakan primer forward ITS5 dan primer reverse ITS4. Pencarian homologi sekuens daerah ITS rDNA menggunakan program basic local alignment search tool BLAST. Pembuatan pohon filogenetik menggunakan metode Neighbor Joining, model dua parameter Kimura dan bootstrap sebanyak 1.000 kali replikasi. Lima isolat kapang terpilih diperoleh berdasarkan tipe morfologi yang berbeda dengan kapang dari manuskrip Cina lama asal plot 2, 4, 5, dan 6. Hasil elektroforesis gel produk PCR daerah ITS rDNA menunjukkan lima strain memiliki ukuran fragmen ITS rDNA dengan kisaran 500--700 pb dan DNA sequencing menunjukkan panjang daerah ITS rDNA berkisar 579--610 pb. Lima strain UICC merupakan anggota dari dua kelas Class Eurotiomycetes dan Dothideomycetes , dua ordo Order Eurotiales dan Capnodiales serta tiga famili Family Aspergillaceae, Cladosporiaceae dan Trichocomaceae. Strain UICC 1099 dan UICC 1102 memiliki homologi 99,4 dan 99,8 dengan type strain Aspergillus pseudodeflectus NRRL 6135T. Strain UICC 1103 memiliki homologi 99,7 dengan type strain Cladosporium colocasiae ATCC 200944 T. Strain UICC 1101 memiliki homologi 99,8 dengan type strain Penicillium coffeae NRRL 35363T. Strain UICC 1100 memiliki homologi 99,4 dengan type strain Penicillium mallochii DAOM 239917T. Lima strain UICC merupakan fungi anamorf dan bersifat xerofilik. ......The objective of this study was to identify moulds isolated from two deteriorated old Chinese manuscripts from plot 1 Ruang Naskah Central Library Universitas Indonesia Depok based on sequence data of internal transcribed spacer regions of ribosomal DNA ITS rDNA . Sterile cotton swab was used to obtain samples and culture dependent method was used to isolate moulds. Forward primer ITS5 and reverse primer ITS4 were used to amplify ITS rDNA region and sequencing the DNA. Basic Local Alignment Search Tool BLAST program was used to determine the sequence homology of ITS rDNA region. A phylogenetic tree was constructed by Neighbor Joining method with Kimura rsquo s two parameter model and bootstrap with 1,000 replicates. Five selected mould isolates were obtained based on the morphological type differences compared to moulds from old Chinese manuscripts from plot 2, 4, 5, and 6. Gel electrophoresis showed that the fragment lengths of ITS rDNA region from five strains were on the range of 500 700 bp and DNA sequencing showed that the length variations of ITS DNA fragments were 579 to 610 bp. The five UICC strains belonged to two classes Class Eurotiomycetes and Dothideomycetes , two orders Order Eurotiales and Capnodiales and three families Family Aspergillaceae, Cladosporiaceae and Trichocomaceae. UICC 1099 and UICC 1102 strains showed 99.4 and 99.8 homologies with their type strain Aspergillus pseudodeflectus NRRL 6135T. UICC 1103 strain has 99.7 homology with its type strain Cladosporium clocasiae ATCC 200944T. UICC 1101 strain has 99.8 homology with its type strain Penicillium coffeae NRRL 35363T. UICC 1100 strain has 99.4 homology with its type strain Penicillium mallochii DAOM 239917T. The five UICC strains are anamorphic and xerophilic fungi.