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Abstrak :
[ABSTRAK
Penelitian mengenai peran siput Terebralia dalam mengurai serasah mangrove dan menyimpan karbon telah dilakukan pada bulan November hingga Desember 2013. Hasil penelitian kepadatan, Terebralia palustris memiliki nilai kepadatan yang paling tinggi yaitu 25 individu/m2. Sedangkan Terebralia sulcata memiliki nilai kepadatan yang lebih rendah yaitu 15 individu/m2. Kandungan karbon yang tersimpan dalam tubuh T. palustris berkisar antara 16,27?18,89% dengan rata-rata sebesar 17,45%. Sedangkan karbon yang tersimpan dalam tubuh T. sulcata berkisar antara 15,98?17,62% dengan rata-rata 16,87%. Potensi penyimpanan karbon oleh T.palustris sebesar 4374 gr C/m2 dan T. sulcata, berpotensi menyimpan karbon sebesar 2609 gr C/m2. Berdasarkan analisis statistik, terdapat korelasi antara panjang maupun berat T. palustris terhadap total karbon dengan masing-masing P= 0,001 dan P= 0,002. Sebaliknya, pada T.sulcata tidak terdapat korelasi antara panjang maupun berat cangkangnya terhadap total karbon yang dikandungnya dengan masing-masing P= 0,071 dan P= 0,289. Penelitian mengenai penguraian seresah dilakukan hanya menggunakan Terebralia dewasa yaitu, Terebralia palustris lebih dari 5 cm dan Terebralia sulcata lebih dari 3 cm. Dalam pengambilan data selanjutnya, dibuat 30 titik kuadran dengan ukuran 0,25 x 0,25 m yang ditentukan secara acak. Rata-rata persentase penguraian serasah oleh T. palustris adalah sebesar 3,48% ± 0,18 /hari untuk R. apiculata dan 8,28% ± 0,13 /hari untuk R. stylosa. Sedangkan T. sulcata mengurai serasah rata-rata sebesar 4,07% ± 0,12 /hari untuk R. apiculata dan 4,93 % ± 0,15 /hari untuk R. stylosa. Hasil penelitian juga menunjukkan bahwa tidak ditemukan korelasi antara panjang dan berat T.palustris dengan persentase penguraiannya. Begitu pula dengan T.sulcata, tidak ditemukan korelasi antara berat dengan laju penguraiannya. Sebaliknya, terdapat korelasi antara panjang T. sulcata dengan persentase penguraiannya.

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ABSTRACT
Research on the role of Terebralia snails in mangrove leaf litter removal and store carbon was held in November and December 2013. Density of research results, Terebralia palustris has the highest density value of 400 individu/m2 . While Terebralia sulcata has a lower density value is 240 individu/m2. Carbon content stored in the body T. palustris ranged from 16.27 to 18.89 % with an average of 17.45 % . While the carbon stored in the body of T. sulcata ranged from 15.98 to 17.62 % with an average of 16.87 %. Potential carbon storage by T. palustris is 4374 g C/m2, whereas T. sulcata potentially storing carbon at 2609 g C/m2. Measurement of litter removal activity was carried out in mangrove dominated by Rhizophora sp. The research location was located in the intertidal mangrove affected part tide. Research conducted using only adult Terebralia such as, Terebralia palustris more than 5 cm and Terebralia sulcata more than 3 cm. In subsequent data collection, we made 30 points quadrant with 0.25 x 0.25 m size was determined at random. The average percentage of leaf litter removal by T. palustris is equal to 3,48% ± 0,18 / day for R. apiculata and 8,28% ± 0,13/ day for R. stylosa . While T. sulcata litter parse an average of 4,07% ± 0,12 / day for R. apiculata and 4,93 % ± 0,15 / day for R. stylosa . The results also show that there were no correlation was found between the length and weight percentage T.palustris with decay. Similar with T.sulcata, no correlation was found between the weight of the rate of decay. Instead, there is a correlation between the length of T. sulcata with the percentage of decay.;Research on the role of Terebralia snails in mangrove leaf litter removal and store carbon was held in November and December 2013. Density of research results, Terebralia palustris has the highest density value of 400 individu/m2 . While Terebralia sulcata has a lower density value is 240 individu/m2. Carbon content stored in the body T. palustris ranged from 16.27 to 18.89 % with an average of 17.45 % . While the carbon stored in the body of T. sulcata ranged from 15.98 to 17.62 % with an average of 16.87 %. Potential carbon storage by T. palustris is 4374 g C/m2, whereas T. sulcata potentially storing carbon at 2609 g C/m2. Measurement of litter removal activity was carried out in mangrove dominated by Rhizophora sp. The research location was located in the intertidal mangrove affected part tide. Research conducted using only adult Terebralia such as, Terebralia palustris more than 5 cm and Terebralia sulcata more than 3 cm. In subsequent data collection, we made 30 points quadrant with 0.25 x 0.25 m size was determined at random. The average percentage of leaf litter removal by T. palustris is equal to 3,48% ± 0,18 / day for R. apiculata and 8,28% ± 0,13/ day for R. stylosa . While T. sulcata litter parse an average of 4,07% ± 0,12 / day for R. apiculata and 4,93 % ± 0,15 / day for R. stylosa . The results also show that there were no correlation was found between the length and weight percentage T.palustris with decay. Similar with T.sulcata, no correlation was found between the weight of the rate of decay. Instead, there is a correlation between the length of T. sulcata with the percentage of decay.;Research on the role of Terebralia snails in mangrove leaf litter removal and store carbon was held in November and December 2013. Density of research results, Terebralia palustris has the highest density value of 400 individu/m2 . While Terebralia sulcata has a lower density value is 240 individu/m2. Carbon content stored in the body T. palustris ranged from 16.27 to 18.89 % with an average of 17.45 % . While the carbon stored in the body of T. sulcata ranged from 15.98 to 17.62 % with an average of 16.87 %. Potential carbon storage by T. palustris is 4374 g C/m2, whereas T. sulcata potentially storing carbon at 2609 g C/m2. Measurement of litter removal activity was carried out in mangrove dominated by Rhizophora sp. The research location was located in the intertidal mangrove affected part tide. Research conducted using only adult Terebralia such as, Terebralia palustris more than 5 cm and Terebralia sulcata more than 3 cm. In subsequent data collection, we made 30 points quadrant with 0.25 x 0.25 m size was determined at random. The average percentage of leaf litter removal by T. palustris is equal to 3,48% ± 0,18 / day for R. apiculata and 8,28% ± 0,13/ day for R. stylosa . While T. sulcata litter parse an average of 4,07% ± 0,12 / day for R. apiculata and 4,93 % ± 0,15 / day for R. stylosa . The results also show that there were no correlation was found between the length and weight percentage T.palustris with decay. Similar with T.sulcata, no correlation was found between the weight of the rate of decay. Instead, there is a correlation between the length of T. sulcata with the percentage of decay., Research on the role of Terebralia snails in mangrove leaf litter removal and store carbon was held in November and December 2013. Density of research results, Terebralia palustris has the highest density value of 400 individu/m2 . While Terebralia sulcata has a lower density value is 240 individu/m2. Carbon content stored in the body T. palustris ranged from 16.27 to 18.89 % with an average of 17.45 % . While the carbon stored in the body of T. sulcata ranged from 15.98 to 17.62 % with an average of 16.87 %. Potential carbon storage by T. palustris is 4374 g C/m2, whereas T. sulcata potentially storing carbon at 2609 g C/m2. Measurement of litter removal activity was carried out in mangrove dominated by Rhizophora sp. The research location was located in the intertidal mangrove affected part tide. Research conducted using only adult Terebralia such as, Terebralia palustris more than 5 cm and Terebralia sulcata more than 3 cm. In subsequent data collection, we made 30 points quadrant with 0.25 x 0.25 m size was determined at random. The average percentage of leaf litter removal by T. palustris is equal to 3,48% ± 0,18 / day for R. apiculata and 8,28% ± 0,13/ day for R. stylosa . While T. sulcata litter parse an average of 4,07% ± 0,12 / day for R. apiculata and 4,93 % ± 0,15 / day for R. stylosa . The results also show that there were no correlation was found between the length and weight percentage T.palustris with decay. Similar with T.sulcata, no correlation was found between the weight of the rate of decay. Instead, there is a correlation between the length of T. sulcata with the percentage of decay.]

Abstrak :
Telah dilakukan penelitian aktivitas antibakteri 38 isolat actinomycetes hasil isolasi dari serasah pada ekosistem bakau di Pulau Pramuka, Kepulauan Seribu, DKI Jakarta. Penapisan dengan plug method menunjukkan bahwa isolat SRM 2 merupakan isolat terunggul dengan Indeks Aktivitas IA antibakteri terhadap Kocuria rhizophila NBRC 12708 1,60, Staphylococcus aureus NBRC 100910 1,18, dan Escherichia coli NBRC 3301 0,71. Produksi senyawa antibakteri dari isolat SRM 2 dilakukan dengan metode still culture pada dua medium berbeda yaitu Cross Streak Media CSM broth dan Production Medium IV PM4 selama 3, 6, dan 9 hari inkubasi. Hasil uji antibakteri dari filtrat medium fermentasi menggunakan diffusion method menunjukkan bahwa medium CSM broth yang diinkubasi selama 6 hari memiliki IA tertinggi terhadap bakteri uji. Ekstraksi senyawa antibakteri dilakukan dengan pelarut etil asetat dan ekstrak kasar diuji pada konsentrasi 20 mg/mL. Hasil uji menunjukkan adanya IA antibakteri hanya terhadap K. rhizophila 0,45 0,08. Sementara hasil uji filtrat medium setelah ekstraksi dengan etil asetat menunjukkan adanya aktivitas antibakteri terhadap K. rhizophila IA: 1,75 0,16, S. aureus IA: 1,18 0,36, Bacillus subtilis NBRC 13719 IA: 0,16 0,05, E. coli IA: 0,79 0,03, dan Pseudomonas sp. IA: 0,10 0,04. Hal tersebut mengindikasikan bahwa isolat SRM 2 menghasilkan senyawa antibakteri bersifat sangat polar dan semi polar. Senyawa sangat polar terlarut dalam filtrat medium, sedangkan senyawa semi polar terekstraksi dengan etil asetat. Hasil analisis dengan High Performance Liquid Chromatography HPLC menunjukkan masih adanya senyawa antibakteri dalam filtrat medium setelah diekstraksi dengan etil asetat. ...... Research about antibacterial activity of 38 actinomycetes isolates from leaf litter of mangrove ecosystem in Pramuka island, Kepulauan Seribu, DKI Jakarta was carried out. Screening of antibacterial activity by plug method showed isolate SRM 2 was the most potential isolate which Activity Index AI against Kocuria rhizophila NBRC 12708 1.60, Staphylococcus aureus NBRC 100910 1.18, and Escherichia coli NBRC 3301 0.71. Production of antibacterial compound from isolate SRM 2 was done by using still culture method on Cross Streak Media CSM broth and Production Medium IV PM 4 for 3, 6, and 9 days incubation. Antibacterial test using filtrate medium by diffusion method showed CSM broth incubated for 6 days has the highest AI against bacterial tested. Extraction of antibacterial compound was done by ethyl acetate solvent and 20 mg ml extracts were tested. Antibacterial test showed AI of antibacterial against K. rhizophila AI 0.45 0.08. Meanwhile antibacterial test using filtrate medium after extraction showed antibacterial activity against K. rhizophila AI 1.75 0.16, S. aureus AI 1.18 0.36, Bacillus subtilis NBRC 13719 AI 0.16 0.05, E. coli AI 0.79 0.03, and Pseudomonas sp. AI 0.10 0.04. Antibacterial coumpounds from isolate SRM 2 were highly polar and semi polar. Highly polar compound dissolved in filtrate medium, while semi polar compound extracted with ethyl acetate. Analysis using High Performance Liquid Chromatography HPLC proved antibacterial compund contained in filtrate medium after extraction by ethyl acetate.