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Putu Indah Paramita Adi Putri
Mikoremediasi air limbah sungai Sentiong, Jakarta dengan isolat jamur ligninolytic inaCC LIPI Cibinong = Micoremediation of Sentiong river wastewater, Jakarta with mushroom isolates ligninolytic inaCC LIPI Cibinong
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kontaminasi dari polutan bandel seperti dimethyl-silanediol sebagai oximemethoxyphenyl. Skrining kemampuan ligninolitik dilakukan pada padatan dan cairan minimal media lignin untuk mendapatkan indeks pertumbuhan dan data aktivitas enzim. Remediasi dilakukan oleh menggunakan konsorsium jamur yang terdiri dari isolat ligninolitik. Mikorediasi diamati dalam skala laboratorium dengan mengukur pH, total karbon (TC), biomassa, dan aktivitas enzim parameter. Komposisi senyawa organik yang mudah menguap dianalisis dengan GC-MS metode. Jamur ligninolitik terbaik di antara lima isolat (IN001, Cyathus striatus IN005, Agrocybe pediades InaCC F1012, Microporus ochrotinctus InaCC F1017, dan Trametes polyzona InaCC F1019) adalah Agrocybe pediades InaCC F1012. Konsorsium diperoleh terdiri dari lima isolat seperti yang disebutkan sebelumnya. Kemampuan mikoremediasi isolat tunggal dan konsorsium dianggap memiliki kemampuan yang sama karena nilai parameter dan hasil analisis GC-MS mendeteksi senyawa yang serupa seperti: dimethyl-silanediol dan oxime-methoxyphenyl. Isolat jamur ligninolitik dapat menjadi solusi untuk remediasi Sungai Sentiong, Jakarta dengan tujuan mengurangi konsentrasi bandel beracun senyawa xenobiotik
Ligninolytic fungi can degrade various Laccase xenobiotic compounds, Manganese Peroxidase (MnP), and Lignin Peroxidase (LiP). The mushroom group is able to be a solution of pollution in the Sentiong River, Jakarta, under conditions contamination from recalcitrant pollutants such as dimethyl-silanediol as oximemethoxyphenyl. Ligninolytic ability screening is done on solids and minimal lignin media to obtain growth index and enzyme activity data. Remediation is done by using a mushroom consortium consisting of ligninolytic isolates. Mycorediation was observed on a laboratory scale by measuring pH, total carbon (TC), biomass, and enzyme activity parameters. The composition of volatile organic compounds was analyzed by GC-MS method. The best ligninolytic fungus among five isolates (IN001, Cyathus striatus IN005, Agrocybe pediades InaCC F1012, Microporus ochrotinctus InaCC F1017, and Trametes polyzone InaCC F1019) is Agrocybe pediades InaCC F1012. The consortium was obtained consisting of five isolates as mentioned earlier. The mycoremediation ability of a single isolate and consortium is considered to have the same ability because the parameter values ​​and the results of GC-MS analysis detect similar compounds such as: dimethyl-silanediol and oxime-methoxyphenyl. Ligninolytic mushroom isolates can be a solution for the remediation of Sentiong River, Jakarta with the aim of reducing the concentration of recalcitrant poisonous xenobiotic compounds."
2019
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UI - Skripsi Membership  Universitas Indonesia Library
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Husnun Hamidah Abbas
Seleksi kapang pendegradasi dioksin dengan pendekatan penghilangan warna remazol brilliant blue R dan poly S-119 serta pengukuran aktivitas enzim ligninolitik = Selection of fungi for dioxin degrading by decolorization of remazol brilliant blue R and poly S-119 also measurement ligninolytic activities
"[Dioksin merupakan senyawa berbahaya yang dapat menyebabkan gangguan kulit, hati, hingga menimbulkan kanker. Degradasi dioksin dapat dilakukan oleh mikroorganisme seperti kapang yang menghasilkan enzim ligninolitik. Penelitian bertujuan untuk mendapatkan kapang yang memiliki enzim ligninolitik sehingga berpotensi dalam mendegradasi dioksin. Aktivitas enzim ligninolitik terlihat dari penghilangan warna pada Remazol Brilliant Blue R (RBBR) dan Poly S-119. Metode penelitian meliputi seleksi pada medium padat dan cair, pengukuran aktivitas enzim ligninolitik, serta identifikasi isolat. Seleksi kapang pada medium padat dilakukan dengan medium yang mengandung RBBR dan Poly S-119. Seleksi cair dilakukan dengan mengukur degradasi warna dan aktivitas enzim ligninolitik (lakase, mangan peroksidase, dan lignin peroksidase). Isolat hasil
seleksi diidentifikasi molekular 28S rRNA menggunakan primer NL-1 dan NL-4. Hasil seleksi padat menunjukkan sembilan isolat dengan zona degradasi, yaitu FIG- KT-540.1; F-IG-KT-539.2; F-IG-PT-6.3; F-IG-PT 1.16; F-IG-PT-2.14; F-IGPT- 2.5; F-IG-PT-2.7; F-IG-PT-3.1; dan F-IG-PT-2.11. Hasil seleksi cair menunjukkan dua isolat memiliki kemampuan mendegradasi warna tinggi yaitu FIG- KT-540.1 sebesar 59% mendegradasi warna RBBR dan F-IG-PT 1.16 sebesar 85% mendegradasi warna Poly S-119. Isolat F-IG-KT-540.1 dan F-IG-PT 1.16 memiliki aktivitas MnP yang tinggi sebesar 0,0132 dan 0,0186 ΔOD/ml sampel/menit. Identifikasi kedua isolat menunjukkan isolat F-IG-KT-540.1 adalah Aspergillus oryzae dengan nilai bootstrap 99 dan isolat F-IG-PT 1.16 adalah Penicillium charlesii dengan nilai bootstrap 98. Kesimpulan yaitu isolat F-IG-KT-
540.1 dan F-IG-PT 1.16 yang memiliki kemampuan tinggi mendegradasi warna berpotensi mendegradasi dioksin. Penelitian lebih lanjut perlu dilakukan untuk mengetahui sinergi antara kedua isolat dalam mendegradasi dioksin.
Dioxins are harmful compounds which can damage skin, liver, and cause cancer. It can be degraded by microorganisms such as fungi with its ligninolytic enzymes. The research aim was to obtain fungi that has ligninolytic enzymes which potentially degrade dioxin. Activity of ligninolytic enzymes was showed from decolorization of Remazol Brilliant Blue R and Poly S-119 dye. Methods of the research include selection on solid medium and liquid medium, measurement of ligninolytic activity, and identification of fungal isolates. Selection on solid medium was carried out using RBBR and Poly S-119 dye. Selection on liquid medium was carried out through measurement on the color degradation and activity of ligninolytic enzymes (laccase, manganese peroxidase, and lignin peroxidase). The potential isolates in liquid selection medium were identified on 28S rRNA with NL-1 and NL-4 primers. The result showed that nine isolates have
the degradation zone in a solid medium. They were F-IG-KT-540.1; F-IG-KT- 539.2; F-IG-PT-6.3; F-IG-PT 1:16; F-IG-PT-2:14; F-IG-PT-2.5; F-IG-PT-2.7; FIG- PT-3.1; and F-IG-PT-2.11. In liquid selection medium, F-IG-KT-540.1 and FIG-
PT 1.16 isolates showed high capability to degrade dyes. Percentage of RBBR degradation in isolate F-IG-KT-540.1 was 59% and percentage of Poly S-119 degradation in isolate F-IG-PT-1.16 was 85%. Both F-IG-KT-540.1 and F-IG-PT 1.16 isolate have high activity of MnP. Activity of MnP of those isolate were 0,0132 and 0,0186 ΔOD/ml/minutes respectively. The result of identification showed that F-IG-KT-540.1 isolate was Aspergillus oryzae with value of
bootstrap 99 and F-IG-PT-1.16 isolate was Penicillium charlesii with value of bootstrap 98. From this research, F-IG-KT-540.1 and F-IG-PT 1.16 isolates which have capability to degrade dyes potential for degrading dioxin. Further research is needed to determine the synergy between isolates F-IG-KT-540.1 and F-IG-PT- 1.16 to degrade dioxin., Dioxins are harmful compounds which can damage skin, liver, and cause cancer.
It can be degraded by microorganisms such as fungi with its ligninolytic enzymes.
The research aim was to obtain fungi that has ligninolytic enzymes which
potentially degrade dioxin. Activity of ligninolytic enzymes was showed from
decolorization of Remazol Brilliant Blue R and Poly S-119 dye. Methods of the
research include selection on solid medium and liquid medium, measurement of
ligninolytic activity, and identification of fungal isolates. Selection on solid
medium was carried out using RBBR and Poly S-119 dye. Selection on liquid
medium was carried out through measurement on the color degradation and
activity of ligninolytic enzymes (laccase, manganese peroxidase, and lignin
peroxidase). The potential isolates in liquid selection medium were identified on
28S rRNA with NL-1 and NL-4 primers. The result showed that nine isolates have
the degradation zone in a solid medium. They were F-IG-KT-540.1; F-IG-KT-
539.2; F-IG-PT-6.3; F-IG-PT 1:16; F-IG-PT-2:14; F-IG-PT-2.5; F-IG-PT-2.7; FIG-
PT-3.1; and F-IG-PT-2.11. In liquid selection medium, F-IG-KT-540.1 and FIG-
PT 1.16 isolates showed high capability to degrade dyes. Percentage of RBBR
degradation in isolate F-IG-KT-540.1 was 59% and percentage of Poly S-119
degradation in isolate F-IG-PT-1.16 was 85%. Both F-IG-KT-540.1 and F-IG-PT
1.16 isolate have high activity of MnP. Activity of MnP of those isolate were
0,0132 and 0,0186 ΔOD/ml/minutes respectively. The result of identification
showed that F-IG-KT-540.1 isolate was Aspergillus oryzae with value of
bootstrap 99 and F-IG-PT-1.16 isolate was Penicillium charlesii with value of
bootstrap 98. From this research, F-IG-KT-540.1 and F-IG-PT 1.16 isolates which
have capability to degrade dyes potential for degrading dioxin. Further research is
needed to determine the synergy between isolates F-IG-KT-540.1 and F-IG-PT-
1.16 to degrade dioxin.]"
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2015
S61564
UI - Skripsi Membership  Universitas Indonesia Library
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Merianda Ramadhian Putri
Studi Mengenai Perbandingan Enzim Ligninolitik dari Jamur Pelapuk Putih dan Bakteri untuk Proses Biodelignifikasi = Study of the Comparison of Ligninolytic Enzymes from White Rot Fungi and Bacteria for the Biodelignification Process
"Keberadaan lignin yang dapat menjadi masalah dalam produksi biofuel dapat diatasi dengan cara delignifikasi. Proses delignifikasi menggunakan mikroorganisme telah menjadi perhatian akhir-akhir ini. Mikroorganisme yang berperan adalah jamur pelapuk putih dan bakteri. Dalam melakukan proses biodelignifikasi, kedua mikroorganisme ini menghasilkan enzim ligninolitik. Enzim ligninolitik antara jamur pelapuk putih dan bakteri menghasilkan persentase delignifikasi dan aktivitas enzim yang berbeda.  Artikel review ini meninjau ulasan mengenai proses biodelignifikasi menggunakan enzim ligninolitik dari jamur pelapuk putih dan bakteri yang akan dibandingkan antara hasil delignifikasi dan aktivitas enzim.  Penulis berharap dapat memberikan gambaran terkait perbandingan antara enzim ligninolitik dari kedua mikroorganisme tersebut.
The existence of lignin which can be a problem in biofuel production can be overcome by delignification. The delignification process using microorganisms has become a concern lately. The microorganisms that play a role are white rot fungi and bacteria. In carrying out the process of biodelignification, these two microorganisms produce ligninolytic enzymes. Ligninolytic enzymes between white rot fungi and bacteria produce different percentages of delignification and enzyme activity. This review article reviews a review of the biodelignification process using ligninolytic enzymes from white rot fungi and bacteria to be compared between the results of delignification and enzyme activity. The author hopes to provide an overview related to the comparison between ligninolytic enzymes of the two microorganisms."
Depok: Fakultas Farmasi Universitas Indonesia, 2020
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UI - Skripsi Membership  Universitas Indonesia Library
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Jessica Jane Judono
Pretreatment Biologis Biomassa Lignoselulosa Menggunakan Jamur Pelapuk Putih = Biological Pretreatment of Lignocellulosic Biomass using White Rot Fungi
"Secara umum, lignoselulosa terdiri dari selulosa, hemiselulosa, dan lignin yang membentuk struktur kompleks yang sulit dihancurkan. Pretreatment bertujuan untuk mendegradasi hemiselulosa dan lignin dari biomassa lignoselulosa serta meningkatkan aksesibilitas enzim ke selulosa yang merupakan bahan baku untuk proses konversi lebih lanjut menjadi produk bernilai tambah. Bahan biomassa memiliki komposisi lignoselulosa yang berbeda-beda yang dapat mempengaruhi proses pretreatment. Masing-masing strategi pretreatment memiliki kelebihan dan keterbatasan tersendiri. Pretreatment biologis merupakan metode yang ramah lingkungan dan hemat energi karena menggunakan mikroorganisme untuk mengatasi sifat rekalsitran biomassa lignoselulosa. Jamur pelapuk putih mampu mendegradasi lignin melalui produksi enzim ligninolitiknya, berupa lakase, lignin peroksidase (LiP), dan mangan peroksidase (MnP). Tujuan penulisan ini adalah memberikan rangkuman penelitian terkait pretreatment biologis menggunakan jamur pelapuk putih dan mekanismenya sebagai mikroorganisme yang dapat mendegradasi lignin. Selain itu, dibahas juga berbagai faktor yang mempengaruhi proses biodelignifikasi. Perlu penelitian lebih lanjut terkait optimalisasi berbagai parameter kondisi kultur agar dapat meningkatkan efisiensi proses pretreatment biologis.
Lignocellulosic biomass mainly consists of cellulose, hemicellulose, and lignin which form complex structures that are difficult to destroy. Pretreatment is significance for the degradation of hemicelluloses and lignin from the lignocellulosic biomass to make cellulose more accessible for further enzymatic process in its conversion into value-added products. Biomass materials have different lignocellulosic compositions which can affect the pretreatment process and requires certain strategy for effective treatment. While each pretreatment strategy has its own strengths and limitations. Biological pretreatment is considered to be an environmentally friendly process with low energy input and low disposal costs for it utilizes lignin-degrading microorganisms to reduce the recalcitrance of lignocellulosic biomass. White rot fungus are able to degrade lignin by producing ligninolytic enzymes, such as laccase, lignin peroxidase (LiP), and manganese peroxidase (MnP). The purpose of this paper is to presents an overview of studies related to biological pretreatment using white rot fungi and its mechanism as a lignin degrading microorganism. In addition, various factors affecting biodelignification process are also discussed. Further research related to parameters optimization of culture conditions is needed in order to increase the efficiency of the biological pretreatment process."
Depok: Fakultas Farmasi Universitas Indonesia, 2020
S70481
UI - Skripsi Membership  Universitas Indonesia Library