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"Latar Belakang: Tulang terus memperbaharui jaringannya dengan mengandalkan keseimbangan resorpsi tulang oleh osteoklas dan deposisi tulang oleh osteoblas. Namun, hal ini dapat terhambat pada kondisi kerusakan tulang yang rumit dan ekstensif. Bone tissue engineering telah berpotensi menjadi alternatif dalam mengatasi masalah regenerasi tulang, salah satunya menggunakan material hidroksiapatit dan gelatin yang bersifat biokompatibel dan osteokonduktif. Pada studi ini, penulis ingin mengetahui pengaruh penambahan propolis pada material hidroksiapatit-gelatin terhadap sekresi protein sel osteoblas.Tujuan: Menganalisis total dan profil protein pada medium kultur sel osteoblas setelah pajanan elusi hidroksiapatit, gelatin, dan propolis untuk melihat aktivitas sel osteoblas.Metode: Medium kultur sel osteoblas diberikan pajanan berupa elusi hidroksiapatit-gelatin-propolis 6% dan diambil sampelnya pada hari ke- 3, 7, 14, dan 21. Kemudian dilakukan uji Bradford untuk melihat total protein dan uji SDS-PAGE untuk melihat profil protein.Hasil: Terdapat perbedaan total protein pada semua kelompok dan ditemukan adanya profil protein berupa COL1A1, bone sialoprotein, RUNX2, dan osteonectin.Kesimpulan: Hasil penelitian menunjukkan terdapat perbedaan antara total dan profil protein medium kultur sel osteoblas pada pemberian elusi hidroksiapatit-gelatin-propolis 6% dibanding kelompok kontrol pada hari ke- 3, 7, 14, dan 21 setelah pajanan.......Background: Bone continue to renew their tissue during life that relies on the correct balance between resorption by osteoclasts and deposition by osteoblasts. However, this can be hindered in conditions of complex and extensive bone destruction. Bone tissue engineering has potential to be an alternative in overcoming the problem of bone regeneration, one of which uses hydroxyapatite and gelatin materials that are biocompatible and osteoconductive. The authors wanted to determine the effect of adding propolis to the hydroxyapatite-gelatin material on the protein secretion of osteoblast cells.Objectives: To analyse total and profile protein on medium culture of osteoblast cells after exposure to elution of hydroxyapatite, gelatin, and propolis to see the activity of osteoblast cells. Methods: Osteoblast cell culture medium was exposed to hydroxyapatite-gelatin-propolis 6% elution and samples were taken on days 3, 7, 14, and 21. Then the Bradford test was performed to see the total protein and SDS-PAGE test to see the protein profile. Results: There were differences of total protein in all groups and found the presence of profile protein, such as COL1A1, bone sialoprotein, RUNX2, and osteonectin.Conclusion: The results showed that there was a difference between the total and protein profile of the osteoblast cell culture medium in the administration of hydroxyapatite-gelatin-propolis 6% elution compared to the control group on days 3, 7, 14, and 21 after exposure."

"Latar belakang. Metode penilaian viabilitas embrio yang masih kurang akurat menjadi tantangan dalam peningkatan keberhasilan fertilisasi in vitro. Kualitas embrio dipengaruhi oleh umur kronologis dan regulator ekspresi gen. Salah satu regulator ekspresi gen tersebut merupakan MicroRNA-135b. MicroRNA-135b sangat potensial untuk dikembangkan lebih lanjut sebagai biomarker kualitas embrio fertilisasi in vitroyang bersifat non-invasif. MicroRNA-135b terekspresi secara stabil di medium kultur embrio. Selain itu, terjadi peningkatan ekspresi MicroRNA-135b di medium kultur embrio aneuploidi dibandingkan medium kultur embrio euploidi pada fertilisasi in vitro. Kondisi aneuploidi pada embrio memiliki korelasi positif dengan umur kronologis dari pasien fertilisasi in vitro. Oleh karena itu, akan diteliti apakah terdapat korelasi antara umur kronologis dan ekspresi MicroRNA-135b pada medium kultur embrio pasien fertilisasi in vitro.Tujuan. 1) Mengetahui sebaran umur kronologis dan ekspresi MicroRNA-135b pada pasien fertilisasi in vitro. 2) Mengetahui korelasi umur kronologis dan ekspresi MicroRNA-135b di medium kultur embrio pasien fertilisasi in vitro. Metode. Studi ini merupakan sebuah studi cross-sectional yang dilakukan pada pasien fertilisasi in vitro Klinik Yasmin RSCM Kencana. Data umur kronologis pasien diperoleh dari data rekam medis pasien. Sampel medium kultur embrio diambil di hari ke-5 prosedur kultur embrio. Selain itu, dilakukan juga pengambilan sampel medium basal sebagai kelompok kontrol. Sampel yang telah diambil akan diperiksa nilai ekspresi MicroRNA-135b dengan analisis kuantitatif real time PCR. Analisis data dilakukan dengan IBM SPSS Statistics 25. Hasil. Total sampel penelitian adalah sebanyak 31 medium kultur embrio dari 11 orang pasien. Umur kronologis dan ekspresi MicroRNA-135b tersebar secara tidak normal. Dari penelitian ini, ditemukan bahwa terdapat korelasi positif bermakna dengan kekuatan statistik sedang antara umur kronologis dengan ekspresi MicroRNA-135b di medium kultur embrio pasien fertilisasi in vitro. Selain itu, ditemukan pula peningkatan relatif ekspresi MicroRNA-135b sebesar 4,9 fold pada medium kultur embrio dibandingkan dengan medium basal fertilisasi in vitro.Kesimpulan. Umur kronologis yang semakin meningkat diikuti dengan peningkatan ekspresi MicroRNA-135b di medium kultur embrio pasien fertilisasi in vitro.......Background. The lack of accuracy in embryo viability assessment methods still become a challenge to increase the in vitro fertilization (IVF) success rate. The quality of embryoinfluenced by the chronological age and gene expression regulator. One of the gene expression regulator is MicroRNA-135b. MicroRNA-135b is very potential to become anoninvasive biomarker of IVF embryo quality. MicroRNA-135b express stably in the spent embryo media. There is an increasement of MicroRNA-135b expression in aneuploidy spent embryo media than euploidy spent embryo media of IVF. Aneuploidy embryo has positive correlation with the IVF patient’s chronological age. Therefore, inthis study, we will determine whether chronological age has correlation with MicroRNA- 135b expression in spent embryo media of IVF patient. Objectives. 1) To determine the chronological age and MicroRNA-135b expressiondistribution at IVF patient. 2) To determine the correlation between chronological age and MicroRNA-135b expression in spent embryo media of IVF patient.Methods. This study is a cross-sectional study which was done to IVF patient of Yasmin Clinic in RSCM Kencana. The chronological age data were collected from the medicalrecords of the patient. The spent embryo media sample were taken at the 5th day of the spent embryo media procedure. We also collected the basal media sample as the controlgroup. The MicroRNA-135b expression were analysed using quantitative real time PCR analysis. The data analysis was using IBM SPSS Statistics 25.Results. There were 31 spent embryo media from 11 patients. The chronological age and MicroRNA-135b expression distribute abnormal. We also found that there was a positivesignificant correlation with moderate statistical power between chronological age and MicroRNA-135b expression in spent embryo media of IVF patient. We also found thatMicroRNA-135b expression increased 4,9 fold in spent embryo media than basal media of IVF. Conclusion. The increase of chronological age followed by the increase of MicroRNA-135b expression in spent embryo media of IVF patient."