Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
Latar Belakang: Pendekatan biologis rekayasa jaringan gigi bertujuan meregenerasi jaringan gigi secara histologis, morfologis dan fungsional. Keterbatasan DPSC gigi manusia, memberikan ide untuk menggunakan jaringan lemak sebagai penghasil sel odontoblas. Tujuan: Menganalisis potensi jaringan lemak sebagai sumber MSCs alternatif untuk menjadi sel odontoblas dengan teknik rekayasa jaringan. Material dan Metode : Kelompok perlakuan ADMSC+rhBMP-2, ADMSC+rhBMP-2+Proterin Pulpa, dan DPSC+rhBMP-2, kontrol ADMSC dan DPSC. Analisis: Stro-1, DMP-1 dan Col-1 untuk karakterisasi odontoblastik, Adhesion Assay, dan Col-1 setelah grafting dengan PRP, PRF, FG. Hasil: Ekspresi seluruh parameter menunjukkan potensi ADMSC dan DPSC yang sama untuk berdiferensiasi ke arah odontoblas. Kesimpulan: Jaringan lemak berpotensi sebagai sumber sel odontoblas dalam proses regenerasi jaringan pulpa. ......Background: Biological approach of dental tissue engineering aims to regenerate tooth structure in histological, morphological, and functional aspect. DPSC limitation of human teeth giving the idea of using adipose tissue to produce odontoblast. Objective: to analyze the potency of adipose tissue as an alternative source of MSCs to produce odontoblast cells by tissue engineering. Materials and Methods: Treatment groups were ADMSC+rhBMP-2, ADMSC+rhBMP- 2+Pulp Protein, and DPSC+rhBMP-2, and control groups of ADMSC and DPSC. Analyzed: Stro-1, DMP-1 and Col-1 for odontoblastic characterization, Adhession Assay and Col-1 after grafted with PRP, PRF, FG. Result: The expression of all markers showed the same potention of ADMSC and DPSC to differentiate towards odontoblast cells. Conclussion: Adipose tissues have the potency as a source of odontoblast cells in the process of pulp tissue regeneration.

Abstrak :
Experimental studies have shown that dental pulp tissue has potential to regenerate dentine in response to adverse stimuli, such as caries and associated operative procedures. However, the potential of dental pulp regeneration seems to be limited by regenerative capacity of the cell involved. In this study, we report the effect of transfection of a recombinant plasmid containing human BMP-2 gene in proliferation and differentiation of dental pulp tissue in vitro. The regenerative capacity was analyzed by ALP production and calcium content. Results showed that the transfected dental pulp cell was able to differentiate into the odontoblast phenotype, indicating the presence of odontoblast progentitor cells in dental pulp tissue.