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"Telah dilakukan penelitian tentang penambahan antioksidan glutathione ke dalam medium pembekuan lambat terhadap kualitas oosit domba garut (Ovis aries) pascakriopreservasi. Tujuan dari penelitian ini adalah untuk mengevaluasi pengaruh penambahan antioksidan glutathione dalam media pembekuan lambat dengan konsentrasi 0,5 mM; 1 mM; 1,5 mM melawan kualitas oosit domba garut. Penelitian dilakukan di Lab. Reproduksi, Pemuliaan dan Kultur Sel Hewan LIPI, Cibinong. Kriopreservasi oosit dari 136 oosit dilakukanmenggunakan krioprotektan 10% etilen glikol dan sukrosa 0,1 M. Evaluasi oosit dilakukan setelah 7 hari penyimpanan dalam nitrogen cair (-196°C), meliputi: morfologi oosit dan viabilitas oosit menggunakan pewarna Hoechst dan propidium pewarna iodida. Berdasarkan hasil penelitian didapatkan persentase oosit normal normal pascakriopreservasi yaitu 0 mM (68,40%), 0,5 mM (66,98%), 1 mM (73,05%), dan 1,5 mM (80,58%). Persentase oosit yang layak pasca-kriopreservasi adalah 0 mM (68,40%), 0,5 mM (69,76%), 1 mM (75,55%), dan 1,5 mM (83,08%). Berdasarkan uji statistik ANOVA, didapatkan hasil bahwa tidak ada perbedaan yang signifikan antar kelompok perlakuan (P > 0,05), namun grafik persentase menunjukkan pola yang cenderung meningkat dengan penambahan konsentrasi antioksidan glutathione. Kesimpulan penelitian berdasarkan hasil yang diperoleh yaitu penambahan antioksidan glutathione dalam media pembekuan lambat tidak berpengaruh terhadap kualitas oosit domba garut pasca-kriopreservasi.Research has been carried out on the addition of glutathione as an antioxidant in slow freezing medium on the quality of post-cryopreserved arrowroot sheep (Ovis aries) oocytes. The aim of this study was to evaluate the effect of adding the antioxidant glutathione in slow freezing medium with a concentration of 0.5 mM; 1 mM; 1.5 mM against arrowroot sheep oocyte quality. The research was conducted in the Lab. Animal Cell Reproduction, Breeding and Culture LIPI, Cibinong. Oocyte cryopreservation of 136 oocytes was performedusing cryoprotectant 10% ethylene glycol and 0.1 M sucrose. Evaluation of oocytes was carried out after 7 days of storage in liquid nitrogen (-196°C), including: oocyte morphology and oocyte viability using Hoechst stain and propidium iodide dye. Based on the results, the percentage of post-cryopreserved normal oocytes was 0 mM (68.40%), 0.5 mM (66.98%), 1 mM (73.05%), and 1.5 mM (80.58%). . The percentage of viable post-cryopreservation oocytes were 0 mM (68.40%), 0.5 mM (69.76%), 1 mM (75.55%), and 1.5 mM (83.08%). Based on the ANOVA statistical test, it was found that there was no significant difference between the treatment groups (P > 0.05), but the percentage graph shows a pattern that tends to increased with the addition of the antioxidant glutathione concentration. The conclusion of the study based on the results obtained was that the addition of the antioxidant glutathione in the slow freezing medium had no effect on the post-cryopreservation quality of arrowroot sheep oocytes."

"Penelitian pemberian maltosa pada visualisasi inti oosit domba garut (Ovis aries L.) setelah maturasi dan pascakriopreservasi menggunakan metode pembekuan lambat telah dilakukan. Tujuan penelitian adalah mengevaluasi kemampuan maltosa dan menentukan konsentrasi maltosa optimum yang menginduksi inti oosit swollen pascamaturasi dan pascakriopreservasi. Pada penelitian ini digunakan domba garut (Ovis aries L.) sebagai hewan model. Penelitian disusun berdasarkan Rancangan Acak Lengkap yang terdiri atas dua uji coba, empat kelompok perlakuan, dan enam kali ulangan. Pada percobaan pertama, dilakukan pematangan oosit hingga tahap Metafase II serta dilakukan pengamatan dengan penambahan berbagai konsentrasi maltosa (0%, 1%, 3%, dan 5%). Kedua, oosit M-II yang diperoleh dilanjutkan ke tahap kriopreservasi dengan metode pembekuan lambat. Selanjutnya, dilakukan pencairan (thawing) serta dilakukan pengamatan dengan penambahan berbagai konsentrasi maltosa (0%, 1%, 3%, dan 5%). Parameter yang digunakan untuk maturasi oosit adalah Metafase II yang ditandai dengan pembentukan badan polar I. Viabilitas inti oosit swollen diamati dengan menggunakan pewarna Hoechst & PI. Sedangkan, inti oosit swollen yang ditandai dengan terdapat zona bening merupakan parameter yang digunakan pascamaturasi dan pascakriopreservasi. Hasil penelitian menunjukkan terdapat perbedaan nyata antarkonsentrasi secara statistik berdasarkan uji normalitas Shapiro-Wilk, uji homogenitas Levene, dan uji sidik ragam ANOVA (P ≥ 0,05) dan konsentrasi maltosa 3% menunjukkan persentase inti oosit swollen pascamaturasi yang tertinggi (66,14%) dan persentase inti oosit swollen pascakriopreservasi yang tertinggi (70,96%). Oleh karena itu, konsentrasi maltosa 3% merupakan konsentrasi optimum yang menyebabkan inti oosit swollen pascamaturasi dan pascakriopreservasi.

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Research on maltose administration on the oocyte nuclear visualization of garut sheep (Ovis aries L.) after maturation and post-cryopreservation using the slow freezing method has been carried out. The aim of this study was to evaluate the ability of maltose and determine the optimum concentration of maltose that induces post-maturation and post-cryopreservation of swollen oocyte nuclear. In this study, garut sheep (Ovis aries L.) were used as animal models. The study was compiled based on completely randomized design consisting of two trials, four treatment groups, and six replications. In the first experiment, to ripen the oocytes in the Metaphase II stage, observations were made with the addition of various concentrations of maltose (0%, 1%, 3%, and 5%). Second, the Metaphase II oocytes obtained were continued to the cryopreservation stage with the slow freezing method. Furthermore, it was thawed and observed by adding various concentrations of maltose (0%, 1%, 3%, and 5%). The parameter used for oocyte maturation was Metaphase II which was characterized by the formation of a Polar Body I. The nuclear viability of swollen oocytes was observed using Hoechst & PI dyes. Meanwhile, swollen oocyte nuclear which is marked by the presence of a clear zone is a parameter used post-maturation and post-cryopreservation. The results showed that there were differences between concentrations statistically based on the Shapiro-Wilk normality test, the Levene homogeneity test, and ANOVA variance test (P ≥ 0.05) and the 3% maltosa concentration showed the highest percentage of post-maturation swollen oocyte nuclear (66.14%) and the highest percentage of post-cryopreservation swollen oocyte nuclear (70.96%). Therefore, the 3% maltose concentration is the optimum concentration to cause swollen post-maturation and post-cryopreservation of oocyte nuclear."

"Sebuah penelitian telah dilakukan dengan menambahkan antioksidan glutathione pada pematangan sedang oosit domba garut. Penelitian dilakukan untuk mengevaluasi penambahan glutathione antioksidan dalam medium pematangan hingga kualitas oosit hingga postavitrifikasi. Sebanyak 129 oosit A ​​dan B berkualitas matang di dalam in vitro Media TCM-199 ditambahkan dengan antioksidan glutathione dengan konsentrasi 0 mM (KK); 0,5 mM (KP1); 1 mM (KP2); dan 1,5 mM (KP3). Oosit matang kemudian di vitrifikasi menggunakan kombinasi cryoprotectant 15% etilen glikol dan 15% dimetil sulfoksida. Evaluasi oosit dilakukan setelah 7 hari penyimpanan dalam cairan nitrogen (-196 ° C), termasuk kematangan oosit berdasarkan keberadaan benda polar, morfologi oosit, dan viabilitas oosit menggunakan pewarna Hoechst dan propidium iodide. Berdasarkan hasil penelitian, persentase yang diperoleh setelah postaturasi oosit matang adalah 0 mM (53,13%); 0,5 mM (55,88%); 1 mM (59,38%); dan 1,5 mM (67,74%). Persentase normal oosit pasca-vitrifikasi yaitu 0 mM (56,25%); 0,5 mM (64,71%); 1 mM (71,88%); dan 1,5 mM (87,10%). Persentase oocytrification hidup adalah 0 mM (56,25%); 0,5 mM (67,65%); 1 mM (71,88%); dan 1,5 mM (87,10%). Hasil uji statistik ANAVA data yang diperoleh tidak berbeda secara signifikan antara kelompok (P> 0,05), namun grafik Persentase menunjukkan pola yang cenderung meningkat dengan penambahan konsentrasi antioksidan glutathione. Kesimpulan dari penelitian berdasarkan hasil yang diperoleh yaitu penambahan antioksidan glutathione ke media pematangan dapat menjaga kualitas oosit ke post-trivirifikasi walaupun tidak signifikan.

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The study has been done by adding the antioxidant glutathione to the medium maturation of arrowroot sheep oocytes. The study was conducted to evaluate the addition antioxidant glutathione in the medium of maturation to oocyte quality up to postavitrification. A total of 129 quality A and B oocytes were matured in vitro insideTCM-199 medium added with antioxidant glutathione with a concentration of 0 mM (KK); 0.5 mM (KP1); 1 mM (KP2); and 1.5 mM (KP3). A mature oocyte then vitrified using a cryoprotectant combination of 15% ethylene glycol and 15%dimethyl sulfoxide. Oocyte evaluation is carried out after 7 days of storage in nitrogen liquid (-196 ° C), including oocyte maturity based on the presence of polar bodies, morphology oocytes, and oocyte viability using Hoechst and propidium iodide dyes. Based on the results of the study, the percentage obtained after postaturation of mature oocytes is 0 mM (53.13%); 0.5 mM (55.88%); 1 mM (59.38%); and 1.5 mM (67.74%). Percentage normal post-vitrified oocytes ie 0 mM (56.25%); 0.5 mM (64.71%); 1 mM (71.88%); and 1.5 mM (87.10%). The percentage of live oocytrification was 0 mM (56.25%); 0.5 mM (67.65%); 1 mM (71.88%); and 1.5 mM (87.10%). ANAVA statistical test results the data obtained did not differ significantly between groups (P> 0.05), however Percentage graphs show patterns that tend to increase with addition concentration of antioxidant glutathione. Conclusions of the study based on the results obtained namely the addition of glutathione antioxidants to the maturation medium can maintain the quality of the oocyte to the post-trivirification although not significant."