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"Pendahuluan: Hilangnya penjangkaran dan relaps pada perawatan ortodontik menjadi hal yang dapat menyebabkan kegagalan perawatan ortodonti dalam jangka panjang. Pemberian gel emulsi berbahan dasar minyak Zoledronate Bisphosphonate (ZOL) dan campuran Virgin Coconut Oil (VCO) secara topikal memiliki potensi meningkatkan apoptosis osteoklas sehingga dapat dipertimbangkan sebagai alternatif penjangkaran dan pencegahan relaps. Tujuan penelitian ini ialah mengetahui stabilitas fisik dan kadar obat gel emulsi ZOL dengan VCO sebagai syarat suatu sediaan dan pengembangan obat baru pada penyimpanan suhu ruangan (25°C) dan suhu pengiriman (40°C). Metode: Gel emulsi disimpan selama 1 bulan pada suhu 25°C dan 40°C. Parameter pengukuran stabilitas, antara lain pH, viskositas, daya sebar, daya lekat, dan kadar obat. Evaluasi dilakukan pada hari pertama, 7, 14, dan 28. Hasil: Uji repeated measure ANOVA pada penyimpanan suhu 25°C dan 40°C menunjukkan terdapat perbedaan bermakna secara statistik pada parameter pH, viskositas, daya lekat, dan kadar obat antar waktu penyimpanan (p<0,05). Pada parameter kadar obat pada penyimpanan suhu 25°C dan 40°C tidak terdapat perbedaan bermakna antar waktu penyimpanan (p>0,05). Sementara, pada penyimpanan gel emulsi ZOL antara suhu 25°C dan 40°C dengan uji t-test independent menunjukkan bahwa nilai pH pada hari ke-7 dan 14, nilai viskositas pada hari ke-14, nilai daya lekat pada hari ke-7, dan nilai kadar pada hari ke-7 dan 14 berbeda bermakna (p>0.05). Sebaliknya, nilai viskositas pada hari ke-7, daya sebar, dan daya lekat pada hari ke- 14 tidak terdapat perbedaan bermakna secara statistik (p>0.05). Kesimpulan: Gel emulsi zoledronate dengan VCO yang disimpan pada suhu 25°C selama 28 hari relatif stabil. Namun perubahan pada nilai pada uji stabilitas relatif konstan dan dalam batas normal mukosa rongga mulut. Gel emulsi zoledronate yang disimpan pada suhu 40°C selama 28 hari disimpulkan tidak stabil.

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Introduction: Loss of anchorage and relapse during and after orthodontic treatment could be the leading causes of an unsuccessful result of orthodontic treatment. Various intra and extra oral application have been used to prevent anchorage loss and relapse in orthodontics with some risks and patient dependent compliance. Topical application of gel emulsion Zoledronate Bisphosphonate (ZOL) with Virgin Coconut Oil (VCO) has a potential to increase the apoptosis of osteoclast to prevent undesirable tooth movement. This study aims to analyze and evaluate the physical stability and drug content of gel emulsion zoledronate, VCO, and preservative agent as a new pharmaceutical drug for one month, stored in a room temperature (25°C) and distribution temperature (40°C). The parameters used for evaluation of ZOL gel emulsion are pH value, viscosity, spread ability, adhesive strength, and drug content. Methods: The ingredients of ZOL gel emulsion consisted of ZOL powder, carboxyl methyl cellulose (CMC), VCO, sodium benzoate, antioxidant butylated hydroxytoluene (BHT), and distilled water. The gel emulsions stored for one month at 25°C and 40°C. The parameters used for stability tests were pH, viscosity, spreadability, adhesive strength, and drug content. The ZOL gel emulsion was evaluated on the 1st day, 7th day, 14th day, and 28th day. Results: The result of this study showed that ZOL gel emulsion was clinically stable over 28 days of storage at 25°C. As for the ZOL gel emulsion that stored at 40°C on the 28th day the gel was not stable. Also, there was no significant difference between ZOL gel emulsion at 25°C and 40°C storage. Conclusion: According to the physical stability and drug content test of ZOL gel emulsion, this study concluded that the ZOL gel emulsion stable in the room temperature (25°C) storage. Organoleptic, pH, viscosity, spreadability, adhesive strength value was also stable and the degradation was constant. It is recommended that the storage of ZOL gel emulsion is in room temperature and also well tightly packed."

"Fase awal pergerakan gigi ortodontik diawali dengan respon inflamasi akut. Proses ini menimbulkan respon dari sel paradental dan sel pertahanan tubuh dengan mensintesis dan melepaskan berbagai biomolekul seperti sitokin. Tumor necrosis factor-a TNF-? merupakan sitokin pro-inflamasi penting yang meregulasi respon awal inflamasi pada pergerakan gigi ortodontik. Tujuan dari penelitian ini adalah untuk menganalisis ekspresi TNF-? dengan membandingkan konsentrasinya pada gingival crevicular fluid GCF antara sistem self-ligating SL dan preadjusted edgewise appliance PEA pada tahap awal perawatan. Metode: Delapan belas pasien usia 15-35 tahun yang berpartisipasi dalam penelitian ini dibagi menjadi dua kelompok eksperimental PEA dan SL dan satu kelompok kontrol tanpa perawatan ortodontik. Pasien dipilih berdasarkan kriteria inklusi: indeks iregularitas Little sebesar 4-9 mm pada anterior maksila dengan indikasi perawatan non-ekstrakasi serta tanpa karies aktif, penyakit periodontal, dan penyakit sistemik terkait kerusakan tulang. Cairan krevikular gingiva subjek diambil pada lima titik di anterior maksila sebelum perawatan dan pada: 1, 24, dan 168 jam setelah aplikasi gaya ortodontik. Konsentrasi TNF-? pada sampel GCF diperiksa menggunakan metode enzyme-linked immunoabsorbent assay ELISA. Hasil: Konsentrasi TNF-? meningkat pada 1 jam dan 24 jam setelah aplikasi gaya ortodontik pada kedua sistem baik pada kelompok SL dan PEA. Konsentrasi TNF-? menurun signifikan pada 168 jam setelah aplikasi gaya ortodontik pada kelompok PEA. Sementara itu, pada kelompok SL konsentrasi TNF-? pada 168 jam tetap meningkat walaupun secara statistik tidak signifikan. Kesimpulan: Konsentrasi TNF-? meningkat pada 1 jam dan 24 jam setelah aplikasi gaya ortodontik pada kelompok PEA dan SL. Pada kelopok PEA, konsentrasi TNF-? menurun signifikaan pada 168 jam, sedangkan pada kelompok SL konsentrasi TNF-? tetap meningkat. Perbedaan konsentrasi TNF-? antara kelompok PEA dan SL mungkin disebabkan oleh perbedaan braket, kawat, dan sistem ligasi yang digunakan antara kedua sistem tersebut.

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The early phase of orthodontic tooth movement begins with acute inflammation response. This processes engender a response on the part of paradental cells and migrating inflammatory cells from periodontal ligament capillaries via the synthesis and release of various biomolecules such as cytokines. Tumor necrosis factor a TNF is an important pro inflammatory cytokine that regulates the early phase of inflammation reaction during orthodontic tooth movement. The aim of the present study was to analyze TNF expression by comparing its concentrations in the gingival crevicular fluid GCF between self ligating SL and preadjusted edgewise appliance PEA systems during the early levelling stage of orthodontic treatment.

Methods: Eighteen patients aged 15 35 years who participated in this study were divided into two experimental groups PEA and SL and control group without orthodontic treatment. Patients were selected according to the inclusion criteria Little irregularity index on maxillary anterior teeth ranging from 4 9 mm non extraction orthodontic treatment for the experimental group no active dental caries, periodontitis, and medical history of bone disorder. The GCF was taken at five sites in the maxilla anterior teeth from each subject just before bracket bonding and at 1, 24, and 168 hours after orthodontic force application. TNF levels in GCF were determined by enzyme linked immunoabsorbent assay ELISA.

Results: The concentration of TNF was significantly higher in the experimental groups than in the control group at 24 hours after force application. TNF levels were significantly decreased at 168 hours after force application in the PEA group. Meanwhile, in the SL group, the level of TNF at 168 hours was still increased, although there was no statistically significant difference.

Conclusion: TNF concentration was increased at 1 hour and 24 hours after orthodontic force application in both the PEA and SL groups. In the PEA group, TNF concentration was significantly decreased at 168 hours, meanwhile in the SL group, this value remained increased at this time point. The differences in TNF concentration between the PEA and SL groups may be caused by their different types of brackets, wires, and ligation methods."