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"The aim of the study was to compare ex vivo the toxic effects of six root canal sealers immediately after mixing or setting on human periodontal ligament fibroblasts (HPdLF). Freshly mixed (I group) or set (allowed to dry for 24 h) (II group) specimens of AH Plus Jet (AH), Apexit Plus (AP), MTA Fillapex (FL), GuttaFlow (GF), MetaSEAL Soft (META), and Tubli-Seal (TS) were prepared. HPdLF were exposed for 24 h to the specimens. 3-(4,5-dimethylthiazolo-2-yl)-2,5-diphenyltetrazolium bromide assay was used to examine the effect of the root canal sealers on mitochondrial metabolic activity. Fluorescein isothiocyanate (FITC)-annexin V (AnV) and propidium iodide staining followed by flow cytometry was used to identify the effects of the materials on cell apoptosis/necrosis. Statistical analyses were performed by one-way ANOVA followed by post hoc tests, and significance was determined at P < 0.05. Most materials from the two groups reduced the viability of the cultured cells compared with the control group (P < 0.05). Statistical analysis showed significant differences in HPdLF viability between the individual materials in each group (P < 0.001). AH and AP induced a significant increase in the percentage of apoptotic cells, while TS, FL, and META elevated the proportion of necrotic cells compared with other materials and the controls (p < 0.05). The cytotoxic effects of the tested root canal sealers (both fresh and set) on HPdLF varied. Both forms of sealers were able to cause toxic effects by inducing apoptosis and necrosis in HPdLF. The cytotoxicity of FL, META, TS was mainly associated with necrosis, while AH and AP with apoptosis."

"Latar Belakang: Penggunaan bahan semen (siler) saluran akar dengan kandungan dan karakteristik berbeda diduga dapat memengaruhi retensi pasak fiber terhadap dinding saluran akar pasca perawatan endodontik. Tujuan : Mengetahui perbedaan kekuatan adhesi pasak fiber pada perawatan saluran akar yang menggunakan siler berbasis resin epoksi dan kalsium silikat. Metode: 30 gigi premolar bawah akar tunggal didekoronasi, dilakukan preparasi saluran akar, lalu dibagi menjadi 3 kelompok; Kelompok 1 (kontrol): gigi tanpa pengisian saluran akar, Kelompok 2: gigi yang menggunakan siler resin epoksi (AH-Plus), dan Kelompok 3: gigi yang menggunakan siler berbasis kalsium silikat (Ceraseal). Setelah pengisian saluran akar, preparasi, dan pemasangan pasak fiber. Selanjutnya gigi dipotong pada area sepertiga tengah akar setebal 2 mm kemudian dilakukan uji push-out bond strength menggunakan Universal Testing Machine. Data dianalisis dengan tes One-way Anova dan post hoc Bonferroni. Hasil: Terdapat perbedaan bermakna nilai push-out bond strength antar kelompok semen resin resin epoksi dan kalsium silikat. Kesimpulan: Gigi yang melalui perawatan saluran akar menggunakan siler resin epoksi memberikan kekuatan adhesi pasak fiber yang lebih baik dibandingkan gigi yang menggunakan siler kalsium silikat.

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Background: The use of root canal sealers with different composition and characteristics is thought to effect the retention of fiber post in root canal walls after endodontic treatment. Objective: To evaluate the difference of the fiber post adhesion strength after endodontic treatment using epoxy resin and calcium silicate based root canal sealer. Methods: 30 samples of single-rooted lower premolar were decoronated, got the root canal prepared, then divided into 3 groups; Group 1 (the controls): samples without root canal filling, Group 2 and 3, the canals were filled with gutta percha using epoxy resin (AH-Plus), and calcium silicate (Ceraseal)-based root canal sealer. After root canal obturation, the gutta percha were partly removed, prepared for post space, and then cemented with the fiber posts. Then 2 mm thick disk were cut from the middle root section and subjected to a push-out bond strength test. Data were analysed using the one-way ANOVA and post hoc Bonferroni test. Result: There was a significant difference in the push-out bond strength value between the epoxy resin and calcium silicate-based root canal sealer groups. Conclusion: The endodontic treated tooth previously using epoxy resin root canal sealer gave better fiber post adhesion strength compared to tooth that used calcium silicate based root canal sealer."