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Abstrak :
Telah dilakukan penelitian untuk mengetahui keragaman genetik udang mantis di perairan pesisir Kabupaten Tangerang selama bulan Januari ? Desember 2015. Pengambilan sampel larva Stomatopoda dilakukan sebanyak 3 kali yaitu pada bulan Februari, Juli dan Sepember pada 4 stasiun penelitian yang terdapat di antara Tanjung Kait dan Muara Sungai Cisadane. Stomatopoda dewasa juga diambil sebanyak 1 kali pengambilan sebagai data konfirmasi dengan identifikasi secara morfologi. Empat spesies udang mantis dewasa berhasil diidentifikasi dari 37 individu yang didadapat. Keempat jenis tersebut antara lain Erugosquilla woodmasoni, Odontodactylus sp., Harpiosquilla harpax, dan Miyakea nepa. Dua puluh empat larva Stomatopoda berhasil didapatkan menggunakan jaring plankton pada 3 kali pengambilan sampel. Hanya 12 larva yang berhasil diamplifikasi gen Cytochrome oxidase sub unit 1 untuk identifikasi molekular menggunakan mesin PCR SimplyAmp Biosystem. Seluruh gen yang berhasil diamplifikasi dikirim ke Macrogen Korea untuk proses sekuensing. Hasil akhir menunjukkan terdapat 4 grup kekerabatan berdasarkan sekuens yang terdapat pada bank gen yaitu Oratosquilla interrupta, Harpiosquilla harpax, Stomatopoda sp. 1 BTN-2013 yang merupakan hasil sekuens penelitian sebelumnya dari Mariana dkk. (2013), dan Oratosquilla oratoria dengan nilai identitas 85% (NCBI 2015). Sebaran Stomatopoda di pesisir Tangerang cenderung menjauhi bagian muara sungai yang ditunjukkan dengan jumlah individu yang didapat lebih banyak pada stasiun 1 dan 3. ...... The research was conducted to know mantis shrimp genetic diversity in Tangerang coastal area for periode of January--Desember 2015. The larva of Stomatopoda was sampled 3 times in February, July and September on 4 sampling stations betwen Tanjung Kait and the estuary of Cisadane river. Mature Stomatopoda was sampled once as confirmation data by morfological identification. Four species of mature Stomatopoda had been identified from 37 individuals, those are Erugosquilla woodmasoni, Odontodactylus sp., Harpiosquilla harpax, and Miyakea nepa. Twenty four larvas were found using plankton net in 3 times of sampling. Only 12 from all larvas have been successfully amplified for the sequence of Cytochrome Oxidase sub unit 1 (CO1) using PCR SimplyAmp Biosystem machine. All amplified gene were sent to macrogen for sequencing procedure. The result shown 4 group closely related to species Oratosquilla interrupta, Harpiosquilla harpax, Stomatopoda sp. 1 BTN-2013 sequence from early research by Mariana et al. (2013), and Oratosquilla oratoria with identities 85% (NCBI 2015). The Distribution of Stomatopoda in Tangerang coastal area tend to getting far from the estuary which are statiun 1 and 3 showed the larger amount of individu.

Abstrak :
Penelitian keragaman genetik udang mantis di Perairan Pelabuhan ratu dan Cirebon telah dilakukan pada bulan Februari ndash; November 2016. Penelitian ini bertujuan untuk mengetahui keragaman genetik udang mantis di perairan Pelabuhan ratu dan Cirebon. Identifikasi udang mantis menggunakan karakter morfologi dan DNA barcoding dengan menggunakan Cytochrome oksidase sub unit I COI . Analisis karakter morfologi menggunakan software PAST v.3.14 Paleontological Statistics dengan metode cluster. Rekonstruksi pohon filogenetik menggunakan software MEGA 6 dengan metode Neighbour Joining berdasarkan model Tamura-3 paramater dengan bootstrap 1000 kali. Hasil penelitian menunjukkan bahwa stomatopoda yang ditemukan dari lokasi pengambilan sampel terdiri atas Harpiosquilla harpax, Oratosquilla oratoria, Oratosquillina gravieri dan Harpiosquilla annandalei. Rata-rata kelimpahan larva Stomatopoda di perairan Cirebon pada stasiun I, II, III dan IV masing-masing 0,047; 0,018; 0,003 dan 0,003 ind/m3sedangkan larva di perairan Pelabuhan ratu hanya ditemukan di stasiun IV sebanyak 0,003 ind/m3. Hasil dendogram karakter morfometrik terdiri atas tiga kelompok, yaitu kelompok H. harpax Cirebon - H.harpax Pelabuhan ratu , kelompok O. oratoria-H. annandalei, dan kelompok O. gravieri. Kesamaan pada kelompok H. harpax dari Cirebon dan Pelabuhan ratu sebesar 94,5 sedangkan H. annandalei ndash; O.oratoria sebesar 92,5 . Hasil rekonstruksi filogenetik yang dibentuk berdasarkan sekuen yang sudah dicocokkan pada Gene bank yaitu terdiri atas 2 genus yaitu Harpiosquilla dan Oratosquilla. ...... The study of genetic diversity mantis shrimp in the Pelabuhan Ratu and Cirebon waters was conducted in February November 2016. This study aimed to determine the genetic diversity of the mantis shrimp in the Pelabuhan Ratu and Cirebon waters. Mantis shrimp was identified using morphological characters and DNA barcoding used Cytochrome Oxidase subunit I COI . Analysis character morphological were done using PAST software v.3.14 Paleontological Statistics cluster method. Reconstruction of the phylogenetic tree used MEGA software 6 with Neighbour Joining method based on the model of Tamura 3 parameters by bootstrapping 1000 times. The results showed that stomatopods found from sampling sites consist of Harpiosquilla harpax, Oratosquilla oratoria, Oratosquillina gravieri and Harpiosquilla annandalei. The average abundance of larvae stomatopoda were found in Cirebon waters at station I, II, III and IV 0,047 0,018 0.003 and 0.003 ind m3, respectively, while in the Pelabuhan ratu water fourth station were found as much as 0,003 ind m3. Dendogram of morphometric character consists of three groups, namely H. harpax Cirebon H. harpax Pelabuhan ratu , O. Oratoria H. annandalei group, and the group O. gravieri. Similarities were found H.harpax group of Cirebon and Pelabuhan Ratu as much as 94.5 while H. annandalei O. oratoria was 92.5 . The results of phylogenetic reconstruction were formed by sequences that have been matched in the Gene bank which consists of two genera, Harpiosquilla and Oratosquilla.

Abstrak :
[ABSTRAK
Telah dilakukan penelitian mengenai identifikasi spesies dan distribusi larva udang mantis di Teluk Banten selama bulan Oktober 2013--November 2013. Penelitian bertujuan untuk mengukur efektivitas aplikasi DNA barcoding dalam identifikasi larva udang mantis dan mempelajari pola distribusinya di Teluk Banten. Larva udang mantis sebanyak 138 individu dikoleksi dengan menggunakan jaring larva dengan besar mulut 30x30 cm2 dan besar jaring sebesar 500 μm dari 6 stasiun penelitian. Daerah COI sebagai penanda DNA barcoding efektif dapat digunakan untuk identifikasi larva udang mantis dengan variasi intraspesies sekuen COI berkisar antara 0,7--2,4%. Distribusi larva udang mantis berpusat di Stasiun 4 yang ditandai dengan tingginya kelimpahan larva udang mantis pada lokasi tersebut (P<0,005; ANOSIM). Ordinasi NMDS dan klusterisasi berdasarkan jarak Bray-Curtis menunjukkan distribusi larva udang mantis dipengaruhi oleh kondisi perairanTeluk Banten. Faktor lingkungan yang memengaruhi kelimpahan larva udang mantis adalah suhu, salinitas dan kecerahan dengan nilai R2 adjusted sebesar 94,5% (P<0,05). Distribusi, kelimpahan, dan komposisi larva udnag mantis di Teluk Banten juga dipengaruhi oleh pola perilaku larva (vertical migration) dan arah arus yang memengaruhi perairan Teluk Banten. Distribusi kelimpahan larva pada lokasi penelitian selama bulan Oktober--November 2013 bergerak kearah barat Teluk Banten.

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ABSTRACT
Planktonic larvae of stomatopoda were collected at six stations in Banten Bay from October 2013 to November 2013, aimed at assessing effectiveness of using COI gene for barcoding stomatopoda larvae and studying its distribution in Banten Bay. A total of 138 stomatopod larvae were obtained by deploying larval trap of 30x30 cm2 mouth diameters and 500 μm mesh size for approximately 10 minutes just beneath the surface. Five species of stomatopod successfully identified using COI gene as barcode marker. Variation of intraspecies for COI gene based on Kimura 2-Parameter (K2P) were found to be ranged from 0,7% to 2,4%. NMDS ordination and Bray-Curtis cluster shown that distribution of stomatopod larvae affected by hydrodynamic on Banten Bay. Larvae abundance at six stations in Banten Bay affected by temperature, salinity, and visibility with score of adjusted R2 is 94,5% (P<0,05). Distribution, abundance, and diversity of stomatopods larvae are affected by vertical migration and current on Teluk Banten water.;Planktonic larvae of stomatopoda were collected at six stations in Banten Bay from October 2013 to November 2013, aimed at assessing effectiveness of using COI gene for barcoding stomatopoda larvae and studying its distribution in Banten Bay. A total of 138 stomatopod larvae were obtained by deploying larval trap of 30x30 cm2 mouth diameters and 500 μm mesh size for approximately 10 minutes just beneath the surface. Five species of stomatopod successfully identified using COI gene as barcode marker. Variation of intraspecies for COI gene based on Kimura 2-Parameter (K2P) were found to be ranged from 0,7% to 2,4%. NMDS ordination and Bray-Curtis cluster shown that distribution of stomatopod larvae affected by hydrodynamic on Banten Bay. Larvae abundance at six stations in Banten Bay affected by temperature, salinity, and visibility with score of adjusted R2 is 94,5% (P<0,05). Distribution, abundance, and diversity of stomatopods larvae are affected by vertical migration and current on Teluk Banten water.;Planktonic larvae of stomatopoda were collected at six stations in Banten Bay from October 2013 to November 2013, aimed at assessing effectiveness of using COI gene for barcoding stomatopoda larvae and studying its distribution in Banten Bay. A total of 138 stomatopod larvae were obtained by deploying larval trap of 30x30 cm2 mouth diameters and 500 μm mesh size for approximately 10 minutes just beneath the surface. Five species of stomatopod successfully identified using COI gene as barcode marker. Variation of intraspecies for COI gene based on Kimura 2-Parameter (K2P) were found to be ranged from 0,7% to 2,4%. NMDS ordination and Bray-Curtis cluster shown that distribution of stomatopod larvae affected by hydrodynamic on Banten Bay. Larvae abundance at six stations in Banten Bay affected by temperature, salinity, and visibility with score of adjusted R2 is 94,5% (P<0,05). Distribution, abundance, and diversity of stomatopods larvae are affected by vertical migration and current on Teluk Banten water., Planktonic larvae of stomatopoda were collected at six stations in Banten Bay from October 2013 to November 2013, aimed at assessing effectiveness of using COI gene for barcoding stomatopoda larvae and studying its distribution in Banten Bay. A total of 138 stomatopod larvae were obtained by deploying larval trap of 30x30 cm2 mouth diameters and 500 μm mesh size for approximately 10 minutes just beneath the surface. Five species of stomatopod successfully identified using COI gene as barcode marker. Variation of intraspecies for COI gene based on Kimura 2-Parameter (K2P) were found to be ranged from 0,7% to 2,4%. NMDS ordination and Bray-Curtis cluster shown that distribution of stomatopod larvae affected by hydrodynamic on Banten Bay. Larvae abundance at six stations in Banten Bay affected by temperature, salinity, and visibility with score of adjusted R2 is 94,5% (P<0,05). Distribution, abundance, and diversity of stomatopods larvae are affected by vertical migration and current on Teluk Banten water.]