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Abstrak :
Telah dilakukan pemeriksaan hormon kortikosteron pada tikus betina (Rattus norvegicus) menggunakan FTIR. Penelitian bertujuan mengetahui bilangan gelombang dan nilai absorbansi gugus fungsi spesifik kortikosteron dalam urine selama siklus estrus, dan mengetahui keabsahan FTIR dalam mengukur konsentrasi hormon kortikosteron. Sampel urine dari sepuluh ekor tikus pada saat estrus dan diestrus yang ditentukan melalui ulas vagina dianalisis melalui FTIR. Diperoleh hasil 3 gugus fungsi spesifik dari kortikosteron pada masing-masing bilangan gelombangnya berturut-turut sebagai berikut alkohol (CH2OH) pada 3552 cm-1, hidroksil (OH) pada 3201 cm-1 dan metil (CH3) pada 1375 cm-1. Nilai absorbansi gugus fungsi spesifik kortikosteron diperoleh dan dibandingkan dengan nilai absorbansi keton (C=O), gugus fungsi spesifik dari kreatinin pada bilangan gelombang 1730 cm-1 yaitu 0,24 %. FTIR mampu mendeteksi konsentrasi kortikosteron saat estrus pada 2,00 % ± 0,50 % / % Cr dan saat non estrus pada 1,94 % ± 0,54 % / % Cr. Tidak terdapat perbedaan antara kortikosteron pada kondisi estrus dan non estrus. ......Research in determining corticosterone concentration on female rat (Rattus norvegicus) using FTIR has been conducted. The aim of this research was to determine the wavenumbers and absorbance values of corticosterone's functional groups in urine during estrous cycles, and to verify the FTIR's capability in measuring corticosterone concentration in urine. Urine samples from ten females which were taken at estrus and non estrus determined by vaginal smear, analyzed by FTIR. The results indicated three specific functional groups of corticosterone in each successive wave numbers as follows: alcohol (CH2OH) at 3552 cm-1, hydroxyl (OH) at 3201 cm-1 and methyl (CH3) at 1375 cm-1. Absorbance value of specific functional groups of corticosterone are obtained and compared with absorbance values of ketone group (C = O), specific functional groups of creatinine in the wave number 1730 cm-1 which is 0.24%. FTIR can detection corticosterone concentration at estrus was 2.00 % ± 0.50 % /% Cr and in non-estrus was 1.94 % ± 0.54 % /% Cr. There was no difference between corticosterone in estrus and non-estrus condition.

Abstrak :
Telah dilakukan pemeriksaan hormon progesteron pada tikus betina (Rattus norvegicus, Berkenhout 1769) menggunakan FTIR. Penelitian bertujuan mengetahui bilangan gelombang dan nilai absorbansi gugus fungsi spesifik progesteron dalam darah selama siklus estrus, dan mengetahui keabsahan FTIR dalam mengukur konsentrasi hormon progesteron. Sampel darah dari sepuluh ekor tikus pada fase estrus dan diestrus yang ditentukan melalui ulas vagina dianalisis melalui FTIR dan radioimmunoassay (RIA). Nilai absorbansi dari gugus fungsi spesifik progesteron, yaitu keton (1724 cm-1), metil (1375 cm-1), dan metil-keton (1354 cm-1), dibandingkan dengan nilai absorbansi asam karboksilat (1425 cm-1) pada hemoglobin. Konsentrasi progesteron saat estrus melalui RIA dan FTIR berturut-turut adalah 17,593 ± 4,246 ng/ml dan 0,853 ± 0,310 %; saat diestrus adalah 76.218 ± 4.687 ng/ml dan 1,024 ± 0.268 %.

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Research in determining progesterone concentration on female rat (Rattus norvegicus, Berkenhout 1769) using FTIR has been conducted. The aim of this research was to determine the wavenumbers and absorbance values of progesterone?s functional groups in blood during estrous cycles, and to verify the FTIR?s capability in measuring progesterone concentration in blood. Blood samples from ten females which were taken at estrus and diestrus phases determined by vaginal smear, analyzed by FTIR and Radioimmunoassay (RIA). Absorbance values of progesterone's functional groups, such as ketone (1724 cm¬1), methyl (1375 cm-1), and methyl-ketone (1354 cm-1), were measured relatively to absorbance values of hemoglobin?s carboxylic acid (1425 cm-1). Progesterone concentration at estrus by RIA and FTIR are 17,593 ± 4,246 ng/ml and 0,853 ± 0,310 % respectively; at diestrus are 76.218 ng/ml and 1,024 ± 0.268 % respectively.