Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
[ABSTRAK
Tuberkulosis penyakit infeksi yang mematikan terutama di negara berkembang, termasuk Indonesia. Upaya pencegahan dengan vaksinasi BCG yang dapat meningkatkan respon imun masih belum maksimal. Faktor yang mempengaruhi keberhasilan vaksin adalah status imun host, genetik dan kualitas/kuantitas vaksin. Indonesia sebagai negara kaya tanaman obat, misalnya pasak bumi (Eurycoma longifolia Jack) digunakan sebagai antimalaria serta meningkatkan imun tubuh. Penelitian ini menilai efek ekstrak akar pasak bumi sebagai imunomodulator terutama IFN-ɣ, TNF-α dan IL-10 pada mencit yang diberi vaksin BCG. Eksperimental in vivo dan in vitro darah mencit di kultur pada medium RPMI dengan stimulasi PHA dan BCG. Analisis tidak ada perbedaan yang bermakna (p≥0,05) diantara kelompok perlakuan, analisa dari nilai median terlihat adanya efek ekstrak pasak bumi terhadap peningkatan TNF-α, dan tidak berpengaruh terhadap produksi IFN-γ dan IL-10 pada mencit yang divaksin BCG. Ekstrak akar pasak bumi mempengaruhi respon imun tubuh mencit yang diberi vaksin BCG, walau tidak besar maknanya.

---

ABSTRACT
Tuberculosis is a deadly infectious disease that occurs mainly in developing countries, including Indonesia. Preventive efforts by BCG vaccination to improve the immune response is still not maximum. Factors that affect the success of vaccine are the host immune system, the host genetic and the quality/quantity of the vaccine. Indonesia is rich in medicinal plants, one of those is Pasak Bumi (Eurycoma longifolia Jack) that is widely used as antimalaria and to improve immunity. The research assessed the effects of extracts of Pasak Bumi roots as immunomodulator by measuring IFN-ɣ, TNF-α and IL-10 on mice that were given with BCG vaccine. In vivo and in vitro experiments of mice blood cultured in RPMI medium stimulated with PHA and BCG. The result has shown no significant difference (p≥0,05) among the treatment group, result of median values has shown the effect of Pasak Bumi extract to an increase of TNF-α, and has no effect on the production of IFN-γ and IL-10 in mice vaccinated BCG. Extract of pasak bumi roots affects the immune response of mice that have got BCG vaccine, although it has no significant meaning., Tuberculosis is a deadly infectious disease that occurs mainly in developing countries, including Indonesia. Preventive efforts by BCG vaccination to improve the immune response is still not maximum. Factors that affect the success of vaccine are the host immune system, the host genetic and the quality/quantity of the vaccine. Indonesia is rich in medicinal plants, one of those is Pasak Bumi (Eurycoma longifolia Jack) that is widely used as antimalaria and to improve immunity. The research assessed the effects of extracts of Pasak Bumi roots as immunomodulator by measuring IFN-ɣ, TNF-α and IL-10 on mice that were given with BCG vaccine. In vivo and in vitro experiments of mice blood cultured in RPMI medium stimulated with PHA and BCG. The result has shown no significant difference (p≥0,05) among the treatment group, result of median values has shown the effect of Pasak Bumi extract to an increase of TNF-α, and has no effect on the production of IFN-γ and IL-10 in mice vaccinated BCG. Extract of pasak bumi roots affects the immune response of mice that have got BCG vaccine, although it has no significant meaning.]

Abstrak :
Mitokondria merupakan organel yang memetabolisme besi secara ekstensif, sehingga menjadi target kerusakan yang diinduksi besi pada kondisi hemosiderosis. Produksi reactive oxygen species (ROS) yang tinggi di mitokondria dapat lebih meningkat saat ada besi bebas yang kemudian memicu reaksi Fenton. Produksi ROS yang tinggi dapat menyebabkan stres oksidatif, sehingga regulasi konsentrasi besi harus diatur dengan ketat. Phaleria macrocarpa diketahui mengandung senyawa aktif mangiferin yang telah terbukti memiliki aktivitas kelasi besi, namun belum diketahui apakah dapat bekerja di mitokondria. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak etanol buah Phaleria macrocarpa dalam melindungi mitokondria hati dari kerusakan akibat besi dan kaitannya dengan transporter influks dan efluks besi di hati tikus model hemosiderosis. Penelitian ini menggunakan organ hati tersimpan dari tikus Sprague-Dawleyjantan sebanyak 30 ekor yang dibagi secara acak ke dalam 6 kelompok, yaitu normal (N) dan kelompok hemosiderosis tanpa terapi (Fe), diterapi deferiprone 462,5 mg/kgBB (Fe+DFP), mangiferin 50 mg/kgBB (Fe+M), serta ekstrak etanol buah Phaleria macrocarpa dosis 100 mg/kgBB (Fe+PM100) dan 200 mg/kgBB (Fe+PM200). Dilakukan analisis kadar MnSOD, copy number mtDNA, dan analisis ekspresi mRNA DMT1, ZIP14, MFRN1, MFRN2, ABCB7, dan ABCB8 yang dilaporkan berperan dalam transpor besi ke dalam sel dan mitokondria. Hasil penelitian menunjukkan bahwa ekstrak Phaleria macrocarpa memengaruhi ekspresi gen transporter besi namun tidak dapat memperbaiki penanda kerusakan mitokondria pada organ hati hemosiderosis. ......Mitochondria are organelles that metabolize iron extensively, making them targets for iron-induced damage. The high production of reactive oxygen species (ROS) in mitochondria can be further increased when there is free iron which then triggers the Fenton reaction. High ROS production can cause oxidative stress, so iron concentration regulation must be strictly regulated. Phaleria macrocarpa is known to contain the active compound mangiferin which has been shown to have iron chelation activity, but it is not yet known whether it can work in mitochondria. This study aims to determine the effectiveness of the ethanol extract of Phaleria macrocarpa fruit in protecting liver mitochondria from iron-induced damage and its relation to iron influx and efflux transporters in the liver of hemosiderosis rat models. This study used stored liver organs from 30 male Sprague-Dawley rats which were randomly divided into 6 groups, namely normal (N) and hemosiderosis groups without therapy (Fe), treated with deferiprone 462.5 mg/kgBW (Fe+DFP), mangiferin 50 mg/kgBW (Fe+M), and Phaleria macrocarpa fruit ethanolic extract at a dose of 100 mg/kgBW (Fe+PM100) and 200 mg/kgBW (Fe+PM200). Analysis of MnSOD levels, mtDNA copy number, and analysis of relative mRNA expression of DMT1, ZIP14, MFRN1, MFRN2, ABCB7, and ABCB8 were performed which were reported to play a role in iron transport into cells and mitochondria. The results showed that Phaleria macrocarpa extract has the potential to modulate the expression of iron transporter genes but was not able to ameliorate the mitochondrial damage marker in hemosiderosis liver.

Abstrak :
Latar Belakang: Phaleria macrocarpa (PM) mengandung mangiferin yang memiliki kemampuan sebagai kelator besi dengan membentuk kompleks. Kompleks dapat menekan akumulasi besi pada pasien talasemia yang rutin transfusi. Kondisi besi berlebih dapat mempengaruhi terjadinya cedera organ ginjal. Penelitian bertujuan untuk mengetahui efektivitas ekstrak etanol buah PM sebagai agen kelator besi diamati pada organ ginjal tikus model besi berlebih. Metode: 30 tikus Sprague-Dawley dibagi acak 6 kelompok: normal (N), besi berlebih (KN), besi berlebih diobati Deferiprone dosis 462,5 mg/kgBB (D), besi berlebih diobati mangiferin dosis 50mg /kgBB (M), besi berlebih diobati ekstrak PM dosis 100mg/kgBB (PM100), besi berlebih diobati ekstrak PM dosis 200mg/kgBB (PM200). Injeksi besi diberikan 2kali/minggu selama 3 minggu dilanjutkan 8 minggu bersama pengobatan. Kadar besi ginjal diukur menggunakan AAS. Kadar urea dan kreatinin plasma serta TNF-α ginjal diukur menggunakan kit. Hasil: Mangiferin dari ekstrak terdeteksi pada ginjal tikus model besi berlebih diukur dengan HPLC. Mangiferin dan PM tidak dapat menurunkan kadar besi di organ ginjal dan kadar ureum plasma signifikan. Pengaruh mangiferin dan PM pada kadar kreatinin plasma tidak linier. Mangiferin dan PM dapat menurunkan kadar TNF-α ginjal signifikan dengan KN dan D. Kesimpulan: Mangiferin dan PM memiliki potensi kelator besi dan menurunkan respon inflamasi pada kondisi besi berlebih. ......Background: Mangiferin, active compound in Phaleria macrocarpa (PM), has been shown as an iron chelating agent by forming complexes. The complex can reduce iron accumulation in thalassemia patients receive transfusions. Renal organ failure can be impacted by the high iron. This study aims to determine the effectiveness of ethanolic extract of PM fruit as iron chelating agent observed in the kidney of iron overload rat. Methods: 30 Sprague-Dawley divided randomly six groups: normal (N), iron-overload (KN), iron-overload treated Deferiprone dosage 462,5 mg/kgBW (D), iron-overload treated mangiferin dosage 50mg/kgBW (M), iron-overload treated PM extract dosage 100mg/kgBW (PM100) and iron-overload treated with PM extract dosage 200mg/kgBW (PM200). Iron injection was administered twice/week for 3 weeks, continued 8 weeks with treatment. Kidney iron levels of rats measured using AAS. Plasma urea and creatinine levels as well as renal TNF-α measured using kit. Results: Mangiferin from extract was detected in the kidney of rat iron overload models which measured using HPLC. Mangiferin and PM cannot significantly reduce plasma urea and kidney iron levels. Effect of mangiferin and PM on plasma creatinine levels not linearly. Mangiferin and PM can reduce renal TNF-α levels significantly. Conclusion: Mangiferin and PM have ability as iron chelator and reduce inflammatory response caused iron overload.

Abstrak :
Sel punca adiposa (SPA) telah menjadi sumber pengobatan terkini yang menjanjikan. Penelitian terbaru menunjukkan bahwa mekanisme yang mendasari manfaat sel punca sebagai pengobatan berhubungan dengan efek modulasi parakrin daripada pemberian sel punca itu sendiri. Beberapa penelitian tentang SPA telah melaporkan adanya perubahan dalam jumlah, proliferasi, dan potensi diferensiasi, serta adanya penurunan sitokin dan growth factor pada SPA sehubungan dengan usia donor. Penelitian ini bertujuan untuk menyelidiki perubahan sekretom berdasarkan kelompok usia tua dan muda serta dampaknya terhadap sel fibroblas yang diinduksi UVB. Sel punca adiposa dari donor usia tua dan muda dikultur dan diisolasi untuk diambil sekretomnya. Sel fibroblas yang diinduksi UVB kemudian diinkubasi dengan sekretom sel punca adiposa tersebut. Parameter seperti viabilitas sel dan konsentrasi TGF-β1 pada sekretom sel punca adiposa serta presentase apoptosis, sel mati, ekpresi gen MMP-3 dan COL1A pada sel fibroblas yang diinduksi UVB dan diberikan sekretom usia donor berbeda dievaluasi untuk menilai efek perbedaan usia donor tersebut. Hasil penelitian menunjukan bahwa sekretom sel punca adiposa dengan usia donor berbeda memberikan dampak yang berbeda pada sel fibroblas yang diinduksi UVB. Konsentrasi TGF-β1 pada sekretom SPA asal donor usia muda lebih tinggi dibandingkan dengan SPA donor tua. Persentase apoptosis, sel mati dan ekpresi gen MMP-3 terhadap GAPDH lebih tinggi pada sel fibroblas yang diinduksi UVB dan diberi sekretom SPA donor tua dibandingkan dengan yang diberi sekretom SPA donor muda. Ekpresi gen COL1A lebih rendah pada pada sel fibroblas yang diinduksi UVB dan diberikan sekretom SPA donor tua dibandingkan diberikan sekretom SPA donor muda. Oleh sebab itu, dapat disimpulkan adanya penurunan kemampuan sekretom sel punca adiposa usia donor tua dalam memperbaiki penuaan pada sel fibroblas yang dipajan dengan UVB, berdasarkan morfologi sel, presentase sel apoptosis dan sel mati serta ekpresi MMP-3 dan COL1A terhadap GAPDH. ......Adipose stem cells (ASC) have become a promising source of current treatment. Recent research suggests that the mechanisms underlying the benefits of stem cells as a treatment relate to paracrine modulatory effects rather than the administration of stem cells themselves. Several studies on ASC have reported changes in the number, proliferation and differentiation potential, as well as a decrease in cytokines and growth factors in ASC in relation to donor age. This study aims to investigate secretome changes based on young and old age groups and their impact on UVB- induced fibroblast cells. Adipose stem cells from young and old donors were cultured and isolated for secretome collection. The UVB-induced fibroblast cells were then incubated with the secretome of the adipose stem cells. Parameters such as cell viability and TGF-β1 concentration in the secretome of adipose stem cells as well as the percentage of apoptosis, dead cells, MMP-3 and COL1A gene expression in fibroblast cells induced by UVB and given the secretome of different donor ages were evaluated to assess the effect of differences in donor age. The results showed that the secretome of adipose stem cells with different donor ages had different impacts on UVB-induced fibroblast cells. The concentration of TGF-β1 in the secretome of SPA from young donors was higher compared to ASC from old donors. The percentage of apoptosis, cell death and expression of the MMP-3 gene against GAPDH was higher in fibroblast cells induced by UVB and given the SPA secretome of old donors compared to those given the ASC secretome of young donors. COL1A gene expression was lower in fibroblast cells induced by UVB and given the ASC secretome of old donors compared to those given the ASC secretome of young donors. Therefore, it can be concluded that there is a decrease in the ability of the secretome of adipose stem cells from old donors to improve aging in fibroblast cells exposed to UVB, based on cell morphology, the percentage of apoptotic cells and dead cells as well as the expression of MMP-3 and COL1A against GAPDH.

Abstrak :
Prevalensi talasemia di Indonesia cukup tinggi. Pengobatan talasemia berupa transfusi darah menyebabkan penumpukan besi di organ-organ tubuh dan kerusakan sel. Pemberian deferoxamine sebagai kelator besi banyak menimbulkan efek samping dan mahal. Oleh karena itu, diperlukan pengobatan dengan bahan yang lebih aman dan terjangkau dengan memanfaatkan bahan alami yang memiliki efek kelasi besi. Ektrak air daun Mangifera foetida L. terbukti memiliki efek kelasi terhadap feritin serum penderita talasemia, namun belum diteliti apakah ekstrak etanol daun Mangifera foetida L. juga menunjukkan efek kelasi terhadap feritin. Penelitian ini merupakan studi eksperimental pada tujuh serum pasien talasemia yang dibagi ke dalam tujuh kelompok perlakuan secara ex vivo yaitu: serum, mangiferin, mangiferin ditambah serum, ekstrak etanol 0,5 mg dan 0,75 mg, ekstrak etanol 0,5 mg dan 0,75 mg ditambah serum, namun yang akan dianalisis hanya empat kelompok yaitu: serum, mangiferin ditambah serum, ekstrak etanol 0,5 dan 0,75 mg ditambah serum. Nilai absorbansi setiap kelompok diukur menggunakan spektrofotometer dengan panjang gelombang ()=280 nm. Hasil uji statistik One Way Anova menunjukkan bahwa terdapat perbedaan bermakna antara kelompok perlakuan (p<0,001). Uji Post Hoc didapatkan hasil bahwa ekstrak etanol daun Mangifera foetida L. dosis 0,5 mg memiliki efek kelasi yang sama dengan dosis 0,75 mg (p=0,133). Ekstrak etanol daun Mangifera foetida L. dosis 0,5 mg memiliki efek kelasi yang sama dengan mangiferin murni (p=0,52), sedangkan dosis 0,75 mg memiliki efek kelasi yang berbeda (p=0,001). Perbedaan efek kelasi ini kemungkinan disebabkan oleh perbedaan dosis ekstrak etanol.

---

Thalassemia has a high prevalence in Indonesia. Treatment of thalassemia with blood transfusion causing iron accumulation in the organs and damaging cells. Chelating agent, deferoxamine causes side effects and expensive. Therefore, it?s needed a safer and cheaper treatment by utilizing natural ingredients which have chelating effect. Water extract of Mangifera foetida L. leaf was proven to have the chelating effect on serum thalassemia patients, but there was no research the effects in the ethanol extract. The purpose of this study was to prove the effects of ethanol extract as a chelating agent. This study used an experimental study using seven serums of patients with thalassemia by ex vivo and devided into seven treatments: serum, mangiferin, mangiferin plus serum, etanol extract 0,5 mg and 0,75 mg, etanol extract 0,5 mg and 0,75 plus serum, however only four treatments will be analized: serum, mangiferin plus serum, etanol extract 0,5 mg and 0,75 mg plus serum. They were measured in a spectrophotometer with (SOH)=280 nm. The result by One Way Anova statistical test showed that there was significant difference between groups (p <0.001). Post Hoc test showed that the ethanol extract 0,5 mg has the same chelating effect with ethanol extract 0,75 mg (p = 0,133). Ethanol extract 0,5 mg has the same effect of iron chelation with the mangiferin (p=0,52), while ethanol extract 0,75 mg has different effect (p=0,001). The difference of chelating effect maybe caused by the difference of extract dose.

Abstrak :
Latar Belakang: Hipoksia adalah keadaan defisiensi suplai oksigen ke dalam sel atau jaringan akibat gagalnya sistem respirasi yang membawa oksigen sehingga mengakibatkan kerusakan jaringan. Hati merupakan organ yang sensitif terhadap hipoksia. Keadaan hipoksia dapat menyebabkan kerusakan hati yang mendasari beberapa kondisi patologis jaringan seperti ischemic hepatitis, cirrhosis hepatis. Tanaman Acalypha indica (AI) dan Centella asiatica (CA) telah terbukti memiliki efek antioksidan dan dapat melindungi banyak organ dari kondisi hipoksia. Pada penelitian ini menganalisis pengaruh pemberian kombinasi ekstrak etanol AI dan CA pada pascahipoksia sitemik terhadap fungsi hati, stres oksidatif dan aktivitas antioksidan organ hati. Metode: Dua puluh delapan tikus Spraque-Dawley dibagi secara acak menjadi 7 kelompok. Kelompok kontrol adalah perlakuan tanpa hipoksia, perlakuan enam kelompok lainnya pascahipoksia 7 hari diberikan zat uji sebagai berikut: air, kombinasi dosis 1 dan 2, dosis tunggal AI, dosis tunggal CA dan dosis tunggal vitamin C selama 7 hari. Aktivitas ALT dan AST, kadar MDA, rasio GSH/GSSG dan aktivitas SOD dianalisis dengan statistik menggunakan uji ANOVA yang dilanjutkan multiple comparisons Post Hoc dengan uji Least Significant Difference (LSD) untuk mengetahui kelompok mana yang berbeda, dimana perbedaan dianggap bermakna secara statistik bila p<0.05. Hasil: Tidak ada perbedaan aktivitas ALT dan AST yang bermakna pada semua kelompok. Kadar MDA meningkat pada kelompok pascahipoksia 7 hari dibanding kontrol. Kelompok kombinasi 1 memiliki MDA yang rendah, rasio GSH/GSSG dan aktivitas SOD yang meningkat dibanding dengan kelompok pascahipoksia 7 hari. Kesimpulan: Pemberian zat uji kombinasi 1 memiliki efek perlindungan pada hati tikus terhadap pascahipoksia 7 hari melalui mekanisme stres oksidatif dan antioksidan. ......Background: Hypoxia occurs due to the deficiency of oxygen supply to the cells or tissue caused by the failure of the respiratory system that carries oxygen result in cell or tissue damage. Liver is an organ that has sensitive reaction to hypoxia. Hypoxia may cause liver damage underlying the condition of several pathological tissues, such as; ischemic hepatic, cirrhosis hepatic. Acalypha indica (AI) and Centella asiatica (CA) have been proved to have antioxidant effects and may protect many organs from hypoxic conditions. This study analysed the effect of ethanol extract combination of AI and CA on post-hypoxia toward liver function, oxidative stress and antioxidant activity of the liver. Methode: Twenty-eight Spraque-Dawley rats divided randomly into 7 groups. Controlled group was treated without hypoxia while the six other groups on 7 days-post-hypoxia were given with such substance test as follows: water, dose combination of 1 and 2, single dose of AI, single dose of CA, and single dose of vitamin C. Activities of ALT and AST, MDA, GSH / GSSG ratio and SOD activity were analyzed statistically using ANOVA test followed by Post Hoc multiple comparison by the Least Significant Difference (LSD) to determine which groups was different, where the difference was considered statistically significant at p <0:05. Result: There is no significant difference in the activity of ALT and AST in all groups. MDA levels increased in the 7 days-posthypoxia group compared to the controlled one. The group combination 1 has lower MDA and increasing GSH/GSSG ratio and SOD activity compared with the 7 days-posthypoxia group. Conclusion: The substance of combination 1 test has a protective effect on the rats? liver on 7 days-posthypoxia through oxidative stress and antioxidant mechanisms.

Abstrak :
Peningkatan stres oksidatif pada obesitas mempercepat onset proses senescence. Biji ketumbar (Coriandrum sativum L) mengandung antioksidan alami yang dapat menurunkan stress oksidatif. Penelitian ini bertujuan menilai efek pemberian ekstrak etanol biji ketumbar terhadap stres oksidatif dan cellular senescence pada hati tikus yang diinduksi obes. Induksi obesitas pada tikus Wistar menggunakan pakan tinggi lemak dilakukan selama 12 minggu. Ekstrak biji ketumbar 100mg/kgBB diberikan selama 12 minggu pasca induksi. Berat badan, Indeks Lee, IMT dan profil lipid plasma diukur pada minggu ke 12 dan 24. Setelah nekropsi pada minggu ke 24, diperiksakan MDA hati dan plasma, uji aktvitas spesifik katalase hati, aktivitas SA-β-Gal dan p16INK4A jaringan hati serta profil lipid plasma. Pemberian ekstrak biji ketumbar tidak menurunkan parameter obesitas yaitu berat badan (p=0,44), indeks Lee (p=0,35), IMT (p=0,97) dan kolesterol (p=0,09), namun menurunkan trigliserida (p=0,04) pada tikus obes. Terjadi penurunan MDA plasma (p=0,013) dan hati (p=0,008) disertai peningkatan aktivitas spesifik enzim katalase (p=0,01) pada tikus obes yang diberikan ekstrak biji ketumbar. Peran ekstrak biji ketumbar terhadap perbaikan status stres oksidatif dapat menghambat senescence yang tampak menyebabkan penurunan p16INK4A (p=0,006) namun tidak menurunkan aktivitas SA-β-Gal (p=0,277) pada hati tikus yang diinduksi obesitas ......Increased oxidative stress in obesity accelerates the onset of the senescence. Coriander seed (Coriandrum sativum L) contains natural antioxidants that can reduce oxidative stress. This study aimed to assess the effect of coriander seed ethanolic extract on oxidative stress and cellular senescence in the liver of obese rats. Obesity induction in Wistar rats using high-fat diet was carried out for 12 weeks. Coriander seed extract 100mg/kg BW was administered 12 weeks post-induction. BW, Index Lee, BMI, and plasma lipid were measured at 12nd and 24th weeks. After necropsy at 24th week MDA, catalase-specific activity test, SA-β-Gal activity and p16INK4A of liver tissue, also plasma profile lipids and MDA were examined. Coriander seed extract did not reduce BW (p=0.44), Lee's index (p=0.35), BMI (p=0.97) and cholesterol (p=0.09), but decreased triglycerides (p=0.04) in obese rats. Plasma and liver MDA was decreased (p=0,013 and p=0.008) accompanied by an increase in specific activity of the catalase enzyme (p=0.01) in obese rats given coriander seed extract. The role of coriander seed extract in improving oxidative stress status inhibits senescence which appeared to cause a decrease in p16INK4A (p=0.006) but did not decrease SA-β-Gal activity (p=0.277) in the liver of obese induced rats.

Abstrak :
Sel Punca Mesenkim (SPM) dianggap sebagai sel yang sangat menjanjikan untuk terapi penyakit berdasar inflamasi karena potensi proliferasi multilineagenya, imunogenisitas rendah, migrasi spesifik ke jaringan yang cedera, dan efek imunomodulator potensialnya. Diperlukan data pendukung mengenai potensi imunomodulasi SPM dalam menghadapi kondisi proinflamasi sebelum digunakan dalam uji klinis. Dilakukan desain penelitian eksperimental in vitro kultur sel untuk menilai potensi imunomodulasi SPM yang berasal dari tali pusat (SPM-TP) dan asal jaringan adiposa (SPM-AD). Untuk menciptakan kondisi inflamasi, menggunakan kultur PBMC yang distimulasi dengan mitogen PHA, diikuti oleh kokultur dengan dua jenis SPM. Pengujian proliferasi dengan Ki67 dilakukan dengan qRT-PCR, pengujian sitokin proinflamasi IFN-γ, IL-1β, dan antiinflamasi IL-10 dilakukan dengan metode Luminex dan pengujian sitokin TGF-β dan IDO dilakukan mnggunakan metode ELISA. Hasil studi menunjukkan adanya perbedaan signifikan antara kelompok dengan perlakuan dan tanpa perlakuan, tetapi tidak terdapat perbedaan signifikan diantara dua kelompok perlakuan (SPM- TP dan SPM-AD). Namun, berdasarkan kemampuan untuk menekan proliferasi PBMC terlihat bahwa SPM-TP menunjukkan kemampuan yang lebih baik dibandingkan SPM-AD. ......The Mesenchymal Stem Cells (MSCs) are considered highly promising for inflammatory disease therapy due to their multilineage proliferation potential, low immunogenicity, specific migration to injured tissues, and potential immunomodulatory effects. Supporting data on the immunomodulatory potential of MSCs in facing proinflammatory conditions are required before their use in clinical trials. An experimental in vitro cell culture research design was conducted to assess the immunomodulatory potential of MSCs derived from umbilical cord (UC-MSCs) and adipose tissue (AD-MSCs). To induce inflammatory conditions, peripheral blood mononuclear cells (PBMCs) were stimulated with PHA mitogen, followed by co-culture with the two types of MSCs. Proliferation testing using Ki67 was performed with qRT-PCR, proinflammatory cytokine testing (IFN-γ, IL-1β) and anti-inflammatory cytokine (IL-10) were conducted using the Luminex method, and TGF-β and IDO cytokine testing were performed using the ELISA method. The study results indicated significant differences between the treated and untreated groups, although no significant differences were observed between the two treatment groups (UC-MSCs and AD-MSCs). However, based on the ability to suppress PBMC proliferation, it was evident that UC-MSCs exhibited superior capabilities compared to AD-MSCs.