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Abstrak :
Bakteriosin merupakan senyawa protein yang memiliki efek bakterisida terhadap mikroorganisme lain. Bakteriosin yang dihasilkan bakeri asam laktat sangat potensial untuk digunakan sebagai pengawet makanan alami. Tujuan penelitian ini untuk mengetahui pengaruh medium pertumbuhan MRS, CMG dan CM terhadapaktivitas antimikroba. Leuconostoc mesenteroides Pbac1. Penentuan zona hambatan pertumbuhan terhadap bakteri indikator. Lactobacillus acidilactici dilakukan melalui uji antagonisme dengan metode difusi sumur agar. Hasil penelitian menunjukkan bahwa bakteri indikator yang sensiftif yaitu L. plantarum dan L. acidilactici, sedangkan media terbaik untuk pertumbuhan bkateri indikator sensitif adalah MRS. Diameter zona hambatan terhadap L. plantarum adalah 1.28 cm dan terhadap L. acidilactici adalah 1.23 cm. Media terbaik untuk memproduksi supernatan Leuconostoc mesenteroides Pbac1 degnan aktivitas bakteriosin tertinggi adalah MRS dengandiameter zona hambatan terhadap L. plantarum 1.28 cm. dan terhadap L. acidilactici 1.19 cm. Titer aktivitas bakteriosin adalah 100 AU/ml. Berdasarkan hasil di atas, media MRS digunakan untuk uji aktivitas bakteriosin dari supernata kultur L. mesenteroides Pbac 1 dengansumber karbon berbeda yaitu glukosa, maltosa dan manosa. Hasil menunjukkan bahwa glukosa merupakan sumber karbon terbaik dengan diameter zona hambatan terhdap L. plantarum dan L. acidilactici adalah 1.23 cm. Titer aktivitas bakteriosin terhadap bakteri indikator pada uji tersebut adalah 100 AU/ml.

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Bacteriocin activity of Leuconostoc mesenteroides Pbac1 bacteria on several media. Bacteriocin is a proteinaceous compound that has bactericidal action against microorganisms. Bacteriocins from lactic acid bacteria are very potential as natural food biopreservatives. The aim of the research was to know the infl uence of the growth medium; MRS, CMG, LTB and CM on antimicrobial activity of Leuconostoc mesenteroides Pbac1. Growth inhibition zone determination has been carried out by antagonism assay, as well as diffusion method using Lactobacillus plantarum, Leuconostoc mesenteroides, Staphylococcus aureus, Lactobacillus pentosus and Lactobacillus acidilactici as indicator strains. The results showed that L. plantarum and L. acidilactici were the sensitive indicators. The best growth medium for antagonism assay of the two sensitive indicator bacteria was MRS, which showed inhibition zone diameter of 1.28 cm and 1.23 cm, respectively. The most active supernatant was produced by L. mesenteroides Pbac1 grown on MRS, which inhibited the growth of L. plantarum and L. acidilactici with respective zone diameter of 1.28 cm and 1.19 cm. Bacteriocin titre activity against sensitive indicator bacteria was 100 AU/ml. Based on the result, MRS was further utilized to study the effect of the different carbon sources i.e glucose, maltose and mannose on bacteriocin activity of L. mesenteroides Pbac1. The results showed that glucose was the best carbon source as indicated by the widest diameter of inhibition zone, i.e 1.23 cm, against both indicator strains. Bacteriocin titre activity of the latter study was 100 AU/ml.

Abstrak :
Penelitian ini bertujuan untuk menguji aktivitas produk beta-1,3 glukan hasil ekstraksi dari Agrobacterium sp Bro 1.2.1 isolat lokal galur tipe liar dan galur tipe mutan terhadap penyembuhan luka terbuka pada hewan coba tikus putih galur Sprague Dawley yang dibuat luka terbuka. Ekstraksi produk beta-1,3 glukan dilakukan dengan cara pengendapan dan dilanjutkan dengan pemurnian pada kromatografi kolom sebagai fraksi gradien KCl. Percobaan uji aktivitas dibagi menjadi tujuh kelompok perlakuan yaitu kontrol negatif, kontrol positif dengan Povidon iodium, dua kelompok dari dua produk beta-1,3 glukan komersil dengan dosis masing-masing 0,02 mg/4 cm2 , tiga kelompok beta-1,3 glukan uji dengan dosis masing-masing yaitu 0,02 mg/4 cm2, 0,10 mg/ 4 cm2 dan 0,5 mg/ 4 cm2. Hasil penelitian menunjukkan bahwa terdapat perbedaan yang bermakna antara kontrol negatif dengan kontrol positif dan kelompok uji pada dosis tertinggi 0,5 mg/4 cm2 dibandingkan kelompok kontrol negatif dan kontrol positif (p<0.05) menggunakan analisis statistik beda nyata terkecil.

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Production of beta-1,3 glucan from Agrobacterium and its wound healing activity on white rat. The objective of this study was to determine the activity of beta-1,3 glucan product extracted from local Agrobacterium sp Bro 1.2.1, both wild-type and mutant-type, on opened-wound healing process. Beta-1,3 glucan product was extracted by precipitation, and the purification was carried out by column chromatography as KCl gradient fractions. In this study, white Sprague Dawley rats were employed, and have been treated for opened-wound condition. Seven groups were performed in this experiment, i.e. the negative control, the positive control employing povidone iodine, the two groups of two commercial beta-1,3 glucan with 0,02 mg/4 cm2 each, and the last three groups of beta-1,3 glucan as the test group with 0,02 mg/4 cm2, 0,10 mg/4 cm2 and 0,50 mg/4 cm2, respectively. The result showed significant differences of wound-healing activity performing statistical analysis of the least significance between the negative control, the positive control, as well as the highest dose of the test group of beta-1,3 glucan, at the dose of 0,5 mg/4 cm2 (p<0.05).

Abstrak :
The purpose of this study was to get optimum medium composition and agitation to trypsin-like protease production by Lactobacillus plantarum FNCC 0270. The medium composition and agitation for enzyme production was optimized using Central Composite Design and Response Surface Method with Design Expert software version 7.1.5. Fermentation was carried out in erlenmeyer flask at initial pH 8, 37 °C, with shaker incubator at 87.5 rpm. The results of the best of enzyme activity 1.0 mU/mL, protein levels of 0.557 mg/mL and desirability value of 0.740. Numerical optimization was performed to approach the ideal state of the fermentation or desirability value of 1. The medium composition of fermentation used was: 3.64% baker's yeast, 1.21% glucose, and 0.13% skim milk. The enzyme activity reached was 1.51 mU/mL and protein levels of 0.205 mg/mL. After numerical optimization, the fermentation process was verified using 125 mL Erlenmeyer in shaking incubator at 77 rpm, initial pH 8, 37 °C, 15 h of fermentation. The verification results showed that the enzyme activity and protein levels was 1.273 ± 0.227 mU/mL and 0.248 ± 0.012 mg/mL, respectively.

Optimization of Trypsin-like Protease Production by Lactobacillus plantarum FNCC 0270 using Response Surface Methodology. Tujuan penelitian ini adalah memperoleh komposisi medium dan agitasi optimum untuk produksi protease serupa tripsin oleh Lactobacillus plantarum FNCC 0270. Protease serupa tripsin (PST) dihasilkan oleh L. plantarum FNCC 0270 melalui proses fermentasi dengan optimasi komposisi medium dan agitasi menggunakan Central Composite Design dan Response Surface Methode, dengan software Design Expert versi 7.1.5. Fermentasi dilakukan dalam erlenmeyer pH awal 8 dan suhu 37 °C menggunakan shaker inkubator pada 87,5 rpm. Hasil eksperimen terbaik menunjukkan aktivitas enzim 1 mU/mL, kadar protein 0.557 mg/mL dan diperoleh nilai desirability 0,740. Untuk mendekati keadaan ideal atau nilai desirability 1 dilakukan optimasi melalui simulasi numerik, yaitu fermentasi dengan komposisi baker yeast 3,64%, kadar glukosa 1,21%, konsentrasi skim milk 0,13%, dan agitasi 77 rpm, sehingga diperoleh aktivitas enzim 1,51 mU/mL dan kadar protein 0,205 mg/mL. Setelah optimasi numerik kemudian dilakukan verifikasi fermentasi di laboratorium, dalam erlenmeyer menggunakan shaker inkubator, agitasi 77 rpm, pH awal 8, suhu 37 °C, selama 15 jam. Hasil verifikasi menunjukkan aktivitas enzim dan kadar protein masing-masing 1,273 ± 0,227 mU/mL dan 0,248 ± 0,012 mg/mL.