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Hasil Pencarian

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Amalia Vitariyani Mukti
"Latar Belakang: Virus dengan gejala pneumonia berat teridentifikasi di Wuhan, Cina sebagai virus COVID-19. Sulitnya pemutusan rantai infeksi COVID-19 mengancam seluruh dunia dan dinyatakan sebagai pandemi pada 11 Maret 2020. Akhirnya, berbagai layanan esensial negara seperti pengobatan gigi dan mulut terpaksa dibatasi aktivitasnya sebagai upaya pengendalian infeksi. Namun, rasa sakit yang tidak dapat tertahankan seperti kegawatdaruratan endodontik tidak bisa dihindari dan membutuhkan pertolongan segera. Selama pandemi, RSKGM FKG UI sebagai pusat rujukan pelayanan kedokteran gigi tetap beroperasi dengan memperketat rangkaian pencegahan dan pengendalian infeksi. Maka, identifikasi dan evaluasi kasus kegawatdaruratan endodontik sebelum dengan selama pandemi di RSKGM FKG UI dilakukan.
Tujuan: Mengetahui distribusi dan frekuensi kegawatdaruratan endodontik di Rumah Sakit Khusus Gigi dan Mulut Fakultas Kedokteran Gigi Universitas Indonesia sebelum dan selama pandemi COVID-19.
Metode: Penelitian merupakan penelitian deskriptif retrospektif dan analitik komparatif kategorik menggunakan data sekunder rekam medik.
Hasil: Terdapat 315 kasus kegawatdaruratan endodontik dengan 111 kasus ditemukan sebelum pandemi dan 204 kasus ditemukan selama pandemi. Terdapat dominasi pada kelompok usia produktif, jenis kelampin perempuan, dan tindakan PSA sebelum dan selama pandemi yang diiringi dengan perbedaan dominasi diagnosis kasus, yaitu acute apical abscess sebelum pandemi dan symptomatic irreversible pulpitis selama pandemi. Terdapat perbedaan bermakna antara diagnosis acute apical abscess dengan symptomatic irreversible pulpitis (p-value <0,05) dan perbedaan tidak bermakna antara tindakan perawatan kegawatdaruratan endodontik sebelum dengan selama pandemi.
Kesimpulan: Terjadi peningkatan kasus kegawatdaruratan endodontik selama pandemi COVID-19 yang diiringi dengan penurunan arus kunjungan departemen konservasi.

Background: Virus with symptoms of severe pneumonia was identified in Wuhan, China as COVID-19 virus. The difficulties in controlling the COVID-19 transmission have led to a pandemic which was declared on March 11, 2020. Various essential sectors of the country been restricted at last to control virus transmission. However, intolerable pain such as endodontic emergency is unavoidable and requires immediate help. During the pandemic, RSKGM FKG UI continues to operate as referral centre for dental services by tightening the health protocol. Therefore, it is necessary to identify and evaluate endodontic emergency cases before and during pandemic era at RSKGM FKG UI.
Objective: This study aims to determine the distribution and frequency of endodontic emergency cases in RSKGM FKG UI before and during pandemic era of COVID-19.
Methods: Retrospective descriptive and comparative analytical study is done using secondary data found in patient’s medical record.
Results: There were 315 cases of endodontic emergency with 111 cases found before the pandemic and 204 cases found during the pandemic. Case was dominated by productive age, female, and root canal treatment group before and during pandemic followed by acute apical abscess domination before pandemic and symptomatic irreversible pulpitis domination during pandemic. There was significant difference between acute apical abscess group and symptomatic irreversible pulpitis group (p-value <0,05) accompanied by no significant difference between treatment group before and during pandemic.
Conclusion: Collected data showed an increased cases of endodontic emergencies accompanied by decreased flow of regular visitors at conservative department.
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Depok: Fakultas Kedokteran Gigi Universitas Indonesia, 2021
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UI - Skripsi Membership  Universitas Indonesia Library
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Marbun, Arini Fitria Jamin
"Latar belakang : Pada tahap inflamasi awal pulpa, sitokin dan mediator inflamasi akan disintesis dan disekresikan oleh sel-sel kompleks dentin pulpa. TNF-α adalah sejenis sitokin yang memiliki efek pleiotropik pada berbagai jenis sel dan sebagai pengatur utama respon inflamasi. LPS terhadap pulpa adalah sebagai stimulator poten pada pulpitis yang dapat ditemukan pada jaringan pulpa yang terinflamasi yang menyebabkan pelepasan sitokin inflamasi. Sekretom WJ memiliki potensi dalam regenerasi dan modulasi sistem imun tubuh serta melalui sekresi faktor spesifik. Tujuan : membuktikan Potensi Sekretom Wharton Jelly Terhadap Ekspresi TNF-α Pada hDPSC Yang Terekspos LPS. Metode : Sel punca pulpa (hDPSC) merupakan hasil kultur primer yang telah 80% confluent (telah melalui uji stem cell marker CD90 98%, CD105 99,7% , CD73 94% dan LinNeg 0,5%) dan mencapai P2-3 dilakukan serum starvation 24 jam. Kelompok penelitian ini terdiri dari 5 kelompok, yaitu : DMEM ; DMEM + LPS; DMEM + LPS + USEPro 12,5 % ; DMEM + LPS + USEPro 25 % ; DMEM + LPS + USEPro 50 %. Kemudian dilakukan uji ELISA untuk melihat potensi sekretom WJ dalam menurunkan ekspresi TNF-α pada observasi waktu 24 dan 72 jam. Hasil : Tidak Terdapat perbedaan ekspresi TNF-α (p > 0,05) antara kelompok uji dan kelompok kontrol. Kesimpulan : Walaupun tidak terdapat perbedaan yang bermakna, namun secara substansi sekretom WJ 25% yang paling berpotensi dalam menurunkan ekspresi TNF-α sel punca pulpa yang terekspos LPS.

Background: In the early inflammatory stages of the pulp, cytokines and inflammatory mediators will be synthesized and secreted by cells of the pulp dentin complex. TNF-α is a type of cytokine that has pleiotropic effects on various cell types and is a major regulator of inflammatory responses. LPS is a potent stimulator of pulpitis which can be found in inflamed pulp tissue which causes the release of inflammatory cytokines. The WJ secretome has the potential to regenerate and modulate the body's immune system as well as through the secretion of specific factors. Objective: to prove the potential of the Wharton Jelly Secretome on TNF-α Expression in hDPSCs Exposed to LPS. Method: : Human Dental Pulp stem cells (hDPSC) are the result of primary culture which are 80% confluent (have gone through the stem cell marker test CD90 98%, CD105 99.7%, CD73 94% and LinNeg 0.5%) and reached P2-3 by serum 24 hour starvation. This research group consists of 5 groups, namely: DMEM; DMEM + LPS; DMEM + LPS + USEPro 12.5 % ; DMEM + LPS + USEPro 25 % ; DMEM + LPS + USEPro 50 %. Then an ELISA test was carried out to see the potential of the WJ secretome in reducing TNF-α expression at 24 and 72 hours of observation. Results: There was no difference in TNF-α expression (p > 0.05) between the test group and the control group. Conclusion: Although there was no significant difference, substantially the 25% WJ secretome had the greatest potential in reducing the expression of TNF-α in pulp stem cells exposed to LPS."
Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2024
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UI - Tesis Membership  Universitas Indonesia Library
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Hana Tania Rahmaputri
"Latar Belakang: E. faecalis dan C. albicans adalah mikroorganisme di saluran akar yang mampu bertahan hidup meski sudah dilakukan perawatan saluran akar. Penggunaan larutan irigasi sintetik saat perawatan saluran akar efektif dalam mengeliminasi mikroorganisme di saluran akar namun terbukti toksik terdapat sel punca mesenkim di periapikal. Cuka apel mengandung zat aktif seperti asam organik, flavonoid, fenol, tanin yang bersifat antimikroba dan menghambat pertumbuhan mikroba, namun terbukti tidak toksik bagi sel punca. Tujuan penelitian ini adalah untuk membandingkan efek antimikroba larutan cuka apel berbagai konsentrasi terhadap biofilm E. faecalis dan C. albicans. Metode: Dilakukan paparan larutan cuka apel 0,63%, 1,25%, dan 2,5%, serta kontrol positif NaOCl 2,5% dan kontrol negatif pada biofilm E. faecalis dan C. albicans. Kemudian dilakukan evaluasi efek antimikroba dengan uji hitung koloni dan nilai viabilitas dengan metode MTT Assay. Hasil: Terdapat perbedaan bermakna jumlah koloni dan nilai viabilitas biofilm E. faecalis (p<0,05) dan biofilm C. albicans (p<0,05) antara seluruh konsentrasi larutan cuka apel, kontrol positif, dan kontrol negatif, dengan efek antimikroba larutan cuka apel tertinggi pada konsentrasi 2,5% dan terendah pada konsentrasi 0,63%. Kesimpulan: Larutan cuka apel 2,5% memiliki kemampuan antimikroba dengan nilai viabilitas dan jumlah koloni hampir sebanding dengan kontrol positif NaOCl 2,5%, sehingga larutan cuka apel dapat dipertimbangkan untuk dijadikan alternatif larutan irigasi saluran akar.

Background: E. faecalis and C. albicans are microorganisms in root canals that are able to survive even after root canal treatment. The use of synthetic irrigation solutions during root canal treatment is effective in eliminating microorganisms in the root canal but has been shown to be toxic to periapical mesenchymal stem cells. Apple vinegar contains active substances such as organic acids, flavonoids, phenols, tannins which are antimicrobial and inhibit microbial growth, but have been proven not to be toxic to stem cells. The aim of this study was to compare the antimicrobial effects of apple vinegar solutions of various concentrations on E. faecalis and C. albicans biofilms. Method: Exposure to 0.63%, 1.25% and 2.5% apple vinegar solutions, as well as 2.5% NaOCl positive control and negative control on E. faecalis and C. albicans biofilms were carried out. Then the antimicrobial effect was evaluated using the colony count test and viability value using the MTT Assay method. Result: There were significant differences in the number of colonies and viability values of E. faecalis biofilm (p<0.05) and C. albicans biofilm (p<0.05) between all concentrations of apple vinegar solution, positive control and negative control, with the antimicrobial effect of the apple vinegar solution were highest at a concentration of 2.5% and lowest at a concentration of 0.63%. Conclusion: The 2.5% apple vinegar solution has antimicrobial ability with viability values and colony numbers almost comparable to the 2.5% NaOCl as positive control, so 2,5% apple vinegar solution can be considered as an alternative root canal irrigation solution."
Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2024
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UI - Tugas Akhir  Universitas Indonesia Library
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Audrey Achmadsyah
"Latar Belakang: Ketika perawatan saluran akar gagal, perawatan ulang saluran akar nonbedah, sedapat mungkin, adalah pilihan klinis pertama. Material pengisi saluran akar sebelumnya harus dibuang untuk kemudian dilakukan desinfeksi dan pengisian ulang saluran akar. Terdapat berbagai teknik pembuangan material pengisi, termasuk metode heat carrier, instrumen manual, putar, ultrasonik dan pelarut, atau kombinasi. Penghilangan sepenuhnya material pengisi tidak dapat dilakukan dengan hampir sebagian besar material yang tersisa dari perawatan ulang adalah siler. Siler biokeramik berikatan secara kimiawi dengan membentuk hidroksiapatit dan berpenetrasi ke tubulus dentin untuk menciptakan ikatan mikromekanis. Hal ini membuat pembersihan siler biokeramik sulit dilakukan. Tujuan: Mengevaluasi kebersihan saluran akar dengan berbagai metode pembuangan siler biokeramik menggunakan instrumen putar, kombinasi instrumen putarultrasonik, dan kombinasi instrumen putar-heat carrier pasca obturasi dengan teknik hidraulik dan WVC, yang dianalisis menggunakan Micro-CT. Metode: Empat puluh dua sampel gigi premolar rahang bawah pasca ekstraksi dengan akar lurus dan saluran akar tunggal yang diobturasi dengan siler biokeramik AH Plus® Bioceramic (Dentsply, USA) meggunakan dua teknik, hidraulik dan WVC. Pembuangan material pengisi menggunakan instrumen putar, kombinasi instrumen putar-ultrasonik, dan kombinasi instrumen putar-heat carrier. Pemindaian micro-CT setelah obturasi dan setelah pembuangan material dan dianalisis menggunakan perangkat lunak CTAn. Hasil: Terdapat perbedaan persentase kebersihan saluran akar (p<0,05) yang bermakna antara metode instrumen putar, kombinasi instrumen putar-ultrasonik, dan kombinasi instrumen putar-heat carrier. Kesimpulan: Pembuangan siler biokeramik menggunakan kombinasi instrumen putar-ultrasonik lebih efektif dibandingkan metode instrumen putar dan metode kombinasi instrumen putar-heat carrier. Teknik obturasi tidak mempengaruhi kebersihan saluran akar.

Background: When root canal treatment fails, non-surgical retreatment, if possible, is the first clinical choice. Previous root canal filling materials must be removed for subsequent disinfection and obturation of the root canal. There are various techniques for removing filling materials, including heat carrier methods, manual instruments, rotary, ultrasonic and solvent, or combinations thereof. Complete removal of filling materials cannot be achieved with almost all remaining materials from retreatment being sealer. Bioceramic sealers chemically bond by forming hydroxyapatite and penetrate into dentin tubules to create micromechanical bonds. This makes cleaning of bioceramic sealer difficult. Objective: To evaluate the cleanliness of root canal walls in retreatment with various methods of removing bioceramic sealer using rotary instruments, combination of rotary-ultrasonic instruments, and combination of rotary-heat carrier instruments postobturation with hydraulic and WVC techniques, analyzed using Micro-CT. Methods: Forty-two extracted mandibular premolar samples with straight roots and single root canals obturated with AH Plus® Bioceramic sealer (Dentsply, USA) using two techniques, hydraulic and WVC. Removal of filling material using rotary instruments, combination of rotary-ultrasonic instruments, and combination of rotary-heat carrier instruments. Micro-CT scanning after obturation and after removal of material and analyzed using CTAn software. Results: There is a significant difference (p<0.05) in the percentage of root canal cleanliness between the rotary instrument, the combination with ultrasonic, and the combination with heat carrier.Conclusion: Bioceramic sealer removal methods using a combination of rotary-ultrasonic instruments are more effective than rotary instrument methods and combination of rotary-heat carrier methods. The obturation technique does not affect the cleanliness of root canal."
Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2024
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UI - Tugas Akhir  Universitas Indonesia Library
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Dahmar Luciana Jufri
"Latar belakang: Fokus desinfeksi saluran akar saat ini telah mengalami perubahan dari desinfeksi agresif menjadi proteksi selektif, yang memiliki tujuan utama untuk menciptakan lingkungan yang sesuai, oleh karena itu, bahan irigasi alami sebagai pendamping mulai banyak diteliti potensialnya karena relatif aman. Larutan irigasi dengan kualitas optimal yang dapat membersihkan saluran akar secara menyeluruh sendiri masih belum tersedia dan meskipun ada perkembangan baru di bidang penelitian yang relevan, solusi yang lebih mendekati kualitas ideal belum dikembangkan. Cuka apel dapat dijadikan sebagai bahan alternatif alami yang aman untuk mengeliminasi biofilm sekaligus smear layer, sehingga berpotensi untuk dikembangkan lebih lanjut. Tujuan: Mengetahui perbedaan efek antibakteri bahan irigasi sintetik NaOCl 1,5% dan 2,5% dibandingkan dengan larutan cuka apel terhadap E. faecalis. Metode: Biofilm E. faecalis isolat klinis dibagi menjadi tujuh kelompok sampel untuk dipaparkan dengan bahan uji larutan cuka apel konsentrasi 2,5%, 5%, 10%, kontrol positif NaOCl 1,5%, 2,5% dan kontrol negatif tanpa perlakuan, efek antibakteri dilihat menggunakan metode MTT Assay dan hitung koloni. Hasil: Didapatkan hasil dari kedua uji yang dilakukan bahwa larutan cuka apel konsentrasi 2,5% memiliki efek antibakteri terhadap biofilm E. faecalis tertinggi dibandingkan dengan konsentrasi 5% dan 10%. Kesimpulan: Efek antibakteri larutan cuka apel 2,5%, 5%, dan 10% lebih rendah dibandingkan dengan larutan NaOCl 1,5% dan 2,5% terhadap biofilm E. faecalis.

Introduction: The current focus of root canal disinfection has changed from aggressive disinfection to selective protection, which has the main goal of creating a suitable environment, therefore, natural irrigation materials as a companion have begun to be studied for their potential because they are relatively safe. Irrigation solutions of optimal quality that can thoroughly clean root canals on their own are not yet available and despite new developments in the relevant research area, solutions closer to the ideal quality have not yet been developed. Apple cider vinegar can be used as a safe natural alternative for eliminating biofilm as well as the smear layer, so it has the potential to be developed further. Objective: To determine the differences in the antibacterial effect of synthetic irrigants NaOCl 1,5% and 2,5% compared to apple cider vinegar solution against E. faecalis. Methods: Clinical isolates of E. faecalis biofilm were divided into seven sample groups to be exposed to test materials for apple cider vinegar concentrations of 2,5%, 5%, 10%, positive control NaOCl 1,5%, 2,5%, and negative control without treatment, the antibacterial effect was seen using the MTT Assay method and colony count. Results: The results of the two tests were obtained that a 2.5% concentration of apple cider vinegar had the highest antibacterial effect on E. faecalis biofilm compared to 5% and 10% concentrations. Conclusion: The antibacterial effect of 2,5%, 5%, and 10% apple cider vinegar solutions was lower than 1,5% and 2,5% NaOCl solutions on E. faecalis biofilm.
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Depok: Fakultas Kedokteran Gigi Universitas Indonesia, 2023
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UI - Tugas Akhir  Universitas Indonesia Library
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Stefani Astari Dewi Aryani
"Latar Belakang: Pengisian saluran akar dengan teknologi terbaru siler premixed kalsium silikat pozolan dengan aktivasi ultrasonik dapat mengurangi porositas pengisian saluran akar. Reaksi pozolan menghasilkan siler dengan daya alir yang lebih baik dengan waktu pengerasan yang lebih cepat, terlebih dengan aktivasi ultrasonik yang akan mengurangi terbentuknya porositas dan meningkatkan kerapatan siler terhadap dinding saluran akar. Tujuan: Menganalisis perbedaan kerapatan siler berbasis kalsium silikat dan siler berbasis kalsium silikat pozolan dengan aktivasi ultrasonik dan tanpa aktivasi ultrasonik terhadap dinding saluran akar. Metode: Evaluasi kerapatan siler berbasis kalsium silikat dan kalsium silikat pozolan dengan aktivasi ultrasonik dan tanpa aktivasi ultrasonik terhadap dinding saluran akar pada 32 gigi premolar rahang bawah yang sudah diekstraksi yang dilihat dari persentase volume porositas pengisian saluran akar menggunakan dengan teknologi 3 dimensi Mikro-CT. Hasil: Siler berbasis kalsium silkat pozolan yang diaktivasi ultrasonik menunjukkan kerapatan siler saluran akar terhadap dinding saluran akar terbaik. Kesimpulan: Kerapatan siler berbasis kalsium silikat pozolan terhadap dinding saluran akar lebih baik dibandingkan siler berbasis kalsium silikat. Kerapatan siler yang diaktivasi ultrasonik terhadap dinding saluran akar juga lebih baik dibandingkan dengan yang tidak diaktivasi ultrasonik.

The latest technology of premixed calcium silicate pozzolan sealer with ultrasonic activation can reduce the porosity of root canal filling. Pozzolan reaction allows the sealer with better flow capacity with faster hardening time, especially with ultrasonic activation which will reduce the formation of porosity and increase sealing ability of root canal filling. Objective: Analyzing differences in sealing ability of calcium silicate-based sealers and calcium silicate pozzolan-based sealers with ultrasonic activation and without ultrasonic activation with 3D Micro-CT analysis. Methods: 32 samples of single rooted lower premolar were given root canal filling using gutta percha and calcium silicate-based sealers and with or without ultrasonic activation. Sealing ability was evaluated by measuring total porosity of the root canal filling. Group 1: calcium silicate-based sealer without ultrasonic activation, group 2: calcium silicate-based sealer with ultrasonic activation, group 3: calcium silicate pozzolan-based sealer without ultrasonic activation, and group 4: calcium silicate pozzolan-based sealer with ultrasonic activation. Result: Ultrasonic activated calcium silicate pozzolan-based showed the best sealing ability with lowest porosity. Conclusion: Calcium silicate pozzolan-based sealers have better sealing ability compared to calcium silicate-based sealers. The ultrasonic activated sealer groups also showed better sealing ability compared to the one that was not activated by ultrasonic."
Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2019
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UI - Tugas Akhir  Universitas Indonesia Library
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Atria Mya Kelani
"Latar Belakang: Affected dentin merupakan lapisan yang ditinggalkan pada perawatan karies secara minimal invasif karena dapat diremineralisasi. Lapisan ini masih terdapat ikatan silang kolagen yang intak meskipun, mineral apatit telah hilang. Remineralisasi dentin diregulasi oleh protein non kolagen Dentin Matriks Protein 1 (DMP1). Remineralisasi yang dihasilkan berupa remineralisasi intrafibrilar dan ekstrafibrilar. Remineralisasi intrafibrilar meningkatkan sifat fisik dentin. Guided Tissue Remineralization (GTR) merupakan metode remineralisasi dentin secara intrafibrilar dan ekstrafibrilar menggunakan material analog protein non kolagen. Material ini memiliki fungsi menyerupai DMP1. Salah satu material analog protein non kolagen adalah Carboxymethyl Chitosan/ Amorphous Calcium Phosphate (CMC/ACP). Tujuan:  Mengevaluasi remineralisasi dentin pada permukaan demineralized dentin setelah aplikasi material analog protein non kolagen CMC/ACP. Metode: Dua kelompok dilakukan demineralisasi buatan, salah satunya diaplikasikan material CMC/ACP sedangkan, kelompok lainnya tidak diaplikasikan CMC/ACP. Evaluasi remineralisasi dengan SEM dan EDX. Hasil: Terlihat remineralisasi pada permukaan demineralized dentin dan peningkatan kadar kalsium dan fosfat setelah aplikasi CMC/ACP pada hari ke-7. Perbandingan rerata dua kelompok tidak menunjukkan perbedaan bermakna. Kesimpulan: CMC/ACP memiliki potensi untuk meremineralisasi demineralized dentin.

Background: Affected dentin is a layer has been left during non invasive caries treatment as it can be remineralized. Collagen crosslinking remains intact in this layer, however the apatite minerals have been lost. Dentin remineralization is regulated by a non collagenous protein, Dentin Matrix Protein 1 (DMP1) and resulting intra- and extrafibrillar remineralization. Intrafibrillar remineralization improves physical properties of dentin. Guided Tissue Remineralization (GTR) is a method of collagen dentin remineralization using non collagen protein analog, resulting in intra- and extrafibrillar remineralization.  This material has similar function with DMP1. Carboxymethyl Chitosan/ Amorphous Calcium Phosphate (CMC/ACP) is one of non collagen protein analog.Aim: To evaluate demineralized dentin remineralization after application non collagen protein analog CMC/ACP. Method: Two groups performed artificial demineralization, one of which applied CMC / ACP material whereas, the other group was not applied CMC / ACP. Remineralization was evalutated using SEM and EDX. Result: After 7 days CMC/ACP application, remineralization was observed on the surface of demineralized dentin, which showed a white irregularities surrounding the dentin tubuli. In addition, increasing calcium and phosphate level has been showed experimentally although, the comparison of both group is insignificant. Conclusion: CMC/ACP has a potential for demineralized dentin remineralization.
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Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2018
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UI - Tesis Membership  Universitas Indonesia Library
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Meilisa Rachmawati
"Latar Belakang: Saat ini perawatan pulpa vital mengacu pada konsep minimal intervensi, yaitu dengan meninggalkan lapisan affected dentin saat ekskavasi karena masih terdapat ikatan silang kolagen sehingga dapat dilakukan upaya remineralisasi. Remineralisasi melalui aplikasi material bioaktif Mineral Trioxide Aggregate (MTA) belum dapat menghasilkan karakteristik dentin menyerupai dentin normal karena pembentukan mineral hanya terjadi pada ruang ekstrafibrillar. Penggunaan material analog protein non kolagen dibutuhkan untuk menstabilkan nano Amorphous Calcium Phosphate (ACP) agar dapat memasuki ruang intrafibrillar, salah satunya menggunakan Carboxymethyl Chitosan (CMC).
Tujuan: mengevaluasi remineralisasi dentin pada permukaan dentin terdemineralisasi setelah aplikasi material MTA dan material modifikasi MTA-CMC dengan melihat morfologi permukaan dan komposisi ion pada dentin.
Metode: remineralisasi dilakukan melalui aplikasi material MTA, MTA-CMC 5% dan 10% selama 14 hari pada permukaan sampel dentin yang terdemineralisasi. Pengamatan morfologi dilakukan dengan Scanning Electron Microscope (SEM) dan konfirmasi komposisi ion dengan Energy Dispersive X-ray Spectroscopy (EDX).
Hasil: terdapat perbedaan morfologi pada permukaan dentin setelah dilakukan remineralisasi melalui aplikasi MTA, MTA-CMC 5%, dan MTA-CMC 10% selama 14 hari yang dievaluasi menggunakan SEM. Hasil analisis EDX mengonfirmasi adanya peningkatan kandungan kalsium dan fosfor pada permukaan dentin yang diremineralisasi dengan material MTA-CMC.
Kesimpulan: remineralisasi melalui aplikasi material modifikasi MTA-CMC dapat memengaruhi morfologi permukaan dan komposisi ion pada permukaan dentin yang terdemineralisasi.

Background: Vital pulp treatment refers to the concept of minimal intervention by preserving the affected dentin layer in the excavation process because there are still collagen cross-linked to induce remineralization. Remineralization through the application of bioactive material Mineral Trioxide Aggregate (MTA) produce different dentin characteristics to normal dentin because mineral formation only occurs in the extrafibrillar space. The use of non-collagen protein analog materials is needed to stabilize the nano Amorphous Calcium Phosphate (ACP) to get into the intrafibrillar space, one of which is Carboxymethyl Chitosan (CMC).
Objective: To evaluate dentin remineralization on the surface of demineralized dentin after the application of MTA and MTA-CMC modified material by looking at the surface morphology and ionic composition of the dentin.
Methods: The remineralization was carried out through the application of MTA,MTA-CMC 5%, MTA-CMC 10% materials for 14 days on the surface of demineralized dentin samples. Morphological observations were carried out by Scanning Electron Microscope (SEM) and confirmation of ion composition by Energy Dispersive X-ray Spectroscopy (EDX). Results: there were morphological differences on the dentin surface after remineralization through the application of MTA, MTA-CMC 5%, and MTA-CMC 10% for 14 days which were evaluated using SEM. The results of the EDX analysis confirmed an increase in the content of calcium and phosphorus on the dentin surface remineralized with MTA-CMC material.
Conclusion: remineralization through the application of MTA-CMC modified material can affect the surface morphology and ion composition on the demineralized dentin surface.
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Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2022
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UI - Tugas Akhir  Universitas Indonesia Library
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Badrul Qomar Isroi
"Latar Belakang: Perawatan pulpa vital adalah perawatan konservatif yang bertujuan menjaga pulpa tetap sehat pada gigi yang mengalami trauma, karies, prosedur restorasi dan kelainan anatomi. Mineral Trioxide Aggregate (MTA) merupakan bahan bioaktif yang sering digunakan perawatan pulpa vital. Pengembangan MTA dilakukan untuk meningkatkan karakter fisik dan biokompatibilitasnya. Tujuan: Mengetahui pengaruh aplikasi material MTA dan MTA modifikasi carboxymethyl chitosan (CMC) terhadap viabilitas sel fibroblas. Metode: Sel fibroblas yang telah mengalami serum starvation selama 24 jam, diaplikasikan media kultur berupa material bioaktif yang berbeda yaitu MTA, MTA modifikasi CMC 5% dan 10 % serta DMEM sebagai kontrol. Pengaruh viabilitas sel fibroblas 24 dan 72 jam dinilai dan dihitung menggunakan MTT Assay. Analisis data menggunakan uji statistik One-Way ANOVA, dilanjutkan Post Hoc Bonferonni. Hasil: Terdapat perbedaan bermakna viablitas sel fibroblas pada kelompok MTA, MTA-CMC 5% dan 10% pada periode waktu pengamatan 24 dan 72 jam. Nilai viabilitas terendah terdapat pada kelompok MTA 72 jam sedangkan nilai viabilitas tertinggi terdapat pada kelompok MTA-CMC 5% 24 jam. Kesimpulan: MTA, MTA modifikasi CMC 5% dan 10% memengaruhi viabilitas sel fibroblas pada periode pengamatan 24 dan 72 jam. MTA modifikasi CMC 5% pengamatan 24 jam menghasilkan nilai viabilitas tertinggi dan termasuk kategori material yang tidak toksik.

Background: Vital pulp treatment is a conservative treatment designed to keep the pulp healthy in teeth that have experienced trauma, caries, restoration procedures and anatomical abnormalities. Mineral Trioxide Aggregate (MTA) is one of the bioactive materials that is often used for vital pulp treatment. The development of MTA was carried out to improve its physical characteristics and biocompatibility.Objective: To determine the effect of the application of MTA modified carboxymethyl chitosan (CMC) and MTA materials on the viability of fibroblast cells.Methods: Fibroblast cells that had undergone 24 hours serum starvation were cultured in the different media in in every group MTA and MTA modified CMC concentrations of 5% and 10% and DMEM as a control. The effect of fibroblast cell viability at 24 hours and 72 hours was assessed based on the percentage of live cells calculated using the MTT Assay. Then the results obtained were analyzed using the SPSS statistical test. The distribution of the data was normal so that the One-Way ANOVA parametric test was carried out, followed by Post Hoc Bonferonni.Results: MTT Assay test, it was found that there was a significant difference in the viability of fibroblast cells in the MTA, MTA-CMC groups of 5% and 10% in the 24 and 72 hours observation periods. The lowest viability value was found in the MTA group with 72 hours of observation, while the highest viability value was found in the 5% MTA-CMC group with 24-hour observation. Conclusion: MTA, MTA modified CMC 5% and 10% affected the viability of fibroblast cells in the 24-hour and 72-hour observation periods. MTA modified CMC 5% at 24-hour observation resulted in the highest viability value and included in the category of non-toxic material."
Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2022
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UI - Tugas Akhir  Universitas Indonesia Library
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Meiny Faudah Amin
"Background: Infeksi Endodontik dapat menjadi faktor prediktor penyakit jantung koroner (PJK) aterosklerosis. Penyakit ini terjadi karena merespon proses inflamatori akibat infeksi bakteri Porphyromonas endodontalis. Beberapa literatur mengatakan bahwa etiologi penyakit kardiovaskular disebabkan infeksi kronik. Diduga bakteri Porphyromonas endodontalis yang ada dalam Infeksi Endodontik (IE) dapat juga menjadi faktor prediktor PJK aterosklerosis. Objective: Mengetahui peran dan potensi IE sebagai faktor predikdi PJK aterosklerosis dan juga peran sitokin inflamatori, IL-1 , IL-6, hsCRP, TNF- dalam kaitannya akan hubungan IE dan PJK aterosklerosis. Method: Dilakukan riset rancangan kasus kontrol dengan mengamati ada terpaparnya IE pada penderita PJK aterosklerosis. Dibagi menjadi kelompok subjek PJK aterosklerosis dengan IE sebagai kasus dan kelompok subjek bukan PJK aterosklerosis dan tanpa IE sebagai kontrol. Subjek dilihat intra oralnya terutama jaringan periapikal dan jaringan periodontal, dicatat faktor-faktor tradisional penyebab PJK serta diukur kadar IL-1 , IL-6, hsCRP, TNF- di dalam darah sirkulasi. Dicatat juga adanya gastritis, psoriosis, dan periodontitis. Results: Dianalisis dengan regresi logistik terlhat ada peran yang potensial (p < 0,041) Infeksi Endodontik sebagai penyebab penyakit jantung koroner aterosklerosis. IL-1, IL-6, dan CRP di dalam darah sirkulasi tidak berbeda bermakna setelah dianalisis denam tes Mann- Whitney, walaupun median setiap kelompok variabel lebih tinggi pada kelompok kasus daripada kontrol. Hanya TNF- yang berbeda bermakna (p < 0,019) setelah danalisis dengan uji-t. Conclusion: Infeksi Endodontik mempunyai peran yang potensial menjadi faktor prediktor penyakit jantung koroner aterosklerosis dan mungkin hanya TNF- yang terlibat dalam mekanisme terjadinya PJK karena IE.

Endodontic infection can be a predictive factor of atherosclerosis coronary heart disease (CHD). This disease occurs because it responds to the inflammatory process caused by infection of Porphyromonas endodontalisbacteria. Several literature state that the etiology of cardiovascular disease is caused by chronic infection. Porphyromonas endodontalis bacteria inside Endodontic Infection (EI) is also assumed to be a predictive factor of atherosclerosis CHD. The purpose of this research is to determine the role and potential of EI as a predictive factor of atherosclerosis CHD as well as the role of inflammatory cytokines, IL-1 , IL-6, hsCRP, TNF- in relation to the relationship of EI and atherosclerosis CHD. This research used a case control design research method by observing EI exposure in atherosclerosis CHD patients. Subjects were divided into atherosclerosis CHD subjects with IE as case and non-atherosclerosis CHD subjects without IE as the control. On the subjects, their intraoral was observed, specifically the periapical tissue and periodontal tissue, the traditional factors causing CHD were recorded and the level of IL-1 , IL-6, hsCRP, TNF- in circulating blood was measured. The presence of gastritis, psoriasis, and periodontitis was also recorded. The results of data analysis in this research with logistic regression showed that there was a potential role (p <0.041) of endodontic infection as a cause of atherosclerosis CHD. IL-1, IL-6, and CRP in circulating blood do not differ significantly after being analyzed by Mann- Whitney test even though the median of each group was higher in the case than the control group. It was only TNF- that was significantly different (p <0.019) after being analyzed by t-test. Therefore, the researcher concludes that endodontic infection has a potential role as a predictive factor of atherosclerosis CHD and probably it is only TNF- that is involved in the mechanism of CHD incidence due to IE."
Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2020
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UI - Disertasi Membership  Universitas Indonesia Library
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