Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
Background: Vitamin D role in immune system have been investigated due to the presence of VDR on immune cells. Based on this information deficient level of vitamin D could affect the progression of allergic rhinitis. Unfortunately the data regarding vitamin D status in the normal population and allergic rhinitis patient were very limited in Indonesia. This research was done to provide illustration regarding the status of vitamin D in healthy and allergic rhinitis patients in Jakarta and also to investigate the factor that might affect the level of vitamin D in allergie rhinitis. Methods: This research was an observational cross sectional research. There were 22 subjects used during this research all diagnosed with moderate-severe allergic rhinitis. The study used the Electrochemiluminescence Immunoassay (ECLIA) technique. The data then were analyzed with IBM® SPSS statistic version 22 Results: The difference between the mean vitamin D of patients suffering from allergic rhinitis with healthy controls (12.7±10.3 ng/mL to 15.1±8.1 ng/mL). There was no significant diference in mean vitamin D between the gender groups (Independent Sample T-test p= 0.62). There were no statistical difference between the vitamin D level in patient with different eosinophil count and IL-5 level (IL-5 group: one-way ANOVA: p= 0.897; eosinophil group: One Way ANOVA: p = 0.752). Conclusion: The mean level of vitamin D in allergic rhinitis patients compared to healthy controls showed no significant difference. Comparison studies about level of vitamin D between groups with different gender, IL-5 and eosinophil count showed no significant difference

Abstrak :
ABSTRAK
Latar Belakang. Gangguan kesuburan merupakan masalah kesehatan reproduksi di dunia yang terjadi pada 10-15% pasangan suami istri. Salah satu penyebab gangguan kesuburan atau infertilitas yang dialami pasangan suami istri adalah yang penyebabnya tidak terjelaskan (unexplained). Dikatakan infertil tidak terjelaskan karena pada semua pemeriksaan standar pasangan suami istri termasuk tes ovulasi, patensi tuba dan analisis sperma berada dalam keadaan normal. Sebagian besar masalah infertil tidak terjelaskan dikaitkan dengan gangguan imunologi yang terjadi antara suami istri, dengan adanya perubahan atau pergeseran proporsi subpopulasi limfosit sebagai indikator. Metode. Dilakukan pengambilan sampel darah tepi dan pemisahan sel mononukleus pasangan infertil tidak terjelaskan. Sebelum dilakukan MLR (Mixed Lymphocyte Reaction) sel mononukleus suami istri, dilakukan pemeriksaan typing sel T CD4+, CD8+ dan sel B CD19+ sel mononukleus istri. Sebelum kultur MLR, sel mononukleus suami diinkubasi dengan Mitomycin C. Kultur MLR sel mononukleus suami dan istri selama 72 jam. Dilakukan typing sel T CD4+, CD8+ dan sel B CD19+ sel mononukleus istri setelah kultur. Hasilnya dibandingkan dengan istri pasangan fertil. Hasil. Dari 15 pasangan infertil tidak terjelaskan dan 6 pasangan fertil, tidak terdapat perbedaan bermakna pada rentang usia istri kedua kelompok (p = 0,078). Peningkatan nilai rerata populasi sel T CD4+, CD8+ dan sel B CD19+ sesudah kultur MLR lebih tinggi pada istri pasangan infertil tidak terjelaskan dibandingkan istri pasangan fertil, meskipun secara statistik tidak terdapat perbedaan bermakna (p = 0,223, p = 0,126, p = 0,462). Proporsi peningkatan proliferasi sel T CD4+, CD8+ sesudah kultur MLR pada istri pasangan infertil tidak terjelaskan berbeda bermakna dibandingkan dengan istri pasangan fertil (p = 0,044 dan p = 0,003). Hasil penelitian ini menguatkan dugaan adanya peran imunologi pada sebagian pasangan infertil tidak terjelaskan.

---

ABSTRACT
Background. Infertility is a world reproductive health problem which is occured in 10-15% of the couples. One of the reason of this infertility problem is unexplained. Diagnosis of unexplained infertility is made when all of the basic evaluation including ovulation test, tubal patency and normal sperm analysis are established. The potential cause of unexplained infertility has been described mostly as an immunology problem, whereas there is a change or modulation of lymphocyte subpopulation proportion as an indicator. Method. Peripheral blood and lymphocyte isolation were collected from unexplained infertile couples and fertile couples. Immunotyping of CD4+, CD8+ T cells and CD19+ B cell from wife?s lymphocyte of both group were measured before the mixed lymphocyte reaction (MLR). Before MLR, the husband?s lymphocytes were incubated with Mitomycin C. The MLR between husband and wife?s lymphocytes were cultured for 72 hours. Immunotyping of CD4+, CD8+ T cells and CD19+ B cell after cultured and compared between unexplained infertile couples and controls. Results. From 15 unexplained infertile couples and 6 fertile couples, there were no statistical different in the age ranges between the wife of both group (p = 0,078). The mean number population of CD4+, CD8+ T cells and CD19+ B cell after MLR cultured were higher from the wife of unexplained infertile group compared to the wife of fertile group, but there were no statistical different between them (p = 0,223, p = 0,126, p = 0,462). Increased proportion of CD4+, CD8+ T cells proliferation after MLR cultured from the wife of unexplained infertile couple were significantly different compared to the wife of fertile group (p = 0,044 and p = 0,003). This results suggested that there is a tendency for immunological factor involvement in the pathogenesis of partially unexplained infertility couples

Abstrak :
Pada akhir tahun 2019 dilaporkan beberapa kasus pneumonia di Wuhan, Cina yang disebabkan oleh Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Penyakit yang ditimbulkan oleh virus ini disebut sebagai coronavirus disease 2019 (COVID-19). Jumlah kasus COVID-19 terus mengalami peningkatan dan penyebarannya terjadi pada seluruh kelompok usia termasuk anak-anak. Pemeriksaan real-time reverse transcription-polymerase chain reaction (rRT-PCR) telah diotorisasi oleh Food and Drug Administration (FDA) dan pada pemeriksaan ini dikenal istilah cycle threshold (Ct). Nilai Ct sering dijadikan acuan dalam menentukan tingkat keparahan penyakit pada anak, akan tetapi masih terdapat kontroversi apakah nilai Ct berhubungan dengan tingkat keparahan penyakit. Penelitian ini bermaksud mencari hubungan bermakna antara nilai Ct khususnya gen ORF1ab dan gen N dengan tingkat keparahan COVID-19 pada anak yang dibagi menjadi tingkat keparahan ringan dan sedang sampai kritis. Didapat 52 responden anak dalam penelitian ini, dengan 24 responden terdeteksi gen ORF1ab dan 49 responden terdeteksi gen N. Rerata nilai Ct gen ORF1ab kelompok ringan (33,5 ± 4,4) lebih tinggi dibandingkan dengan kelompok sedang sampai kritis (31,0 ± 6,0). Median nilai Ct gen N kelompok ringan (34,8 [21,3 – 39,4]) lebih tinggi dibandingkan dengan kelompok sedang sampai kritis (31,7 [19,4 – 38,9]). Tidak didapatkan hubungan bermakna baik antara nilai Ct gen ORF1ab (nilai p = 0,25) maupun gen N (nilai p = 0,159) dengan tingkat keparahan COVID-19 pada anak. Berdasarkan hasil penelitian ini, diperlukan berbagai pertimbangan dalam menginterpretasi nilai Ct. ......At the end of 2019, several cases of pneumonia were reported in Wuhan, China caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The disease caused by this virus is known as Coronavirus Disease 2019 (COVID-19). The number of cases of COVID-19 continues to increase and its spread occurs in all age groups including children. Real-time reverse transcription-polymerase chain reaction (rRT-PCR) has been authorized by the Food and Drug Administration (FDA) and in this method a cycle threshold (Ct) value were obtained. The Ct value is often used as a reference in determining the clinical severity in children, but there is still controversy whether the Ct value is related to the clinical severity. This study intends to find a significant relationship between the Ct values, especially the ORF1ab gene and the N gene, with the COVID-19 clinical severity in children which is divided into mild and moderate to critical severity. There were 52 children in this study, with 24 children have ORF1ab gene detected and 49 children have N gene detected. The mean of ORF1ab gene Ct value in mild group (33.5 ± 4.4) was higher than moderate to critical group (31.0 ± 6.0). The median of N gene Ct value ​​in mild group (34.8 [21.3 – 39.4]) was higher than moderate to critical group (31.7 [19.4 – 38.9]). There was no significant relationship between the Ct value of the ORF1ab gene (p value = 0.25) and the N gene (p value = 0.159) with COVID-19 clinical severity in children. Based on the results of this study, various considerations are needed in interpreting the Ct value.

Abstrak :
ABSTRAK
Sepsis merupakan penyakit umum di perawatan intensif dan hampir 1/3 pasien yang dirawat di ICU adalah pasien sepsis. Banyak penelitian dilakukan untuk mencari penanda sepsis yang handal dan jumlah apoptosis limfosit mulai banyak diteliti sebagai penanda sepsis. Apoptosis limfosit terjadi mulai 24 jam pertama setelah onset sepsis. Saat ini belum terdapat data yang menunjukkan dapat digunakannya jumlah apoptosis limfosit sebagai penanda prognostik sepsis. Tujuan penelitian ini adalah mengetahui dapat tidaknya jumlah apoptosis limfosit digunakan sebagai penanda prognostik pada pasien sepsis berat

Desain penelitian adalah uji prognosis secara prospektif, terdiri dari 30 pasien sepsis berat dibagi berdasarkan mortalitas 14 hari, yaitu 15 pasien hidup dan 15 pasien meninggal. Diagnosis sepsis berdasarkan modifikasi definisi sepsis oleh International Sepsis Definitions Conference 2001. Jumlah apoptosis limfosit dihitung menggunakan metode flowcytometry dengan reagen antibodi monoklonal CD45 berlabel PerCP, Annexin V berlabel FITC, dan Propidium Iodide. Pada kedua kelompok tersebut dicatat data karakteristik subyek dan dilakukan penghitungan jumlah apoptosis limfosit

Rerata jumlah apoptosis limfosit pada kelompok pasien hidup adalah 0,992% dengan simpang baku 0,44% dan rerata jumlah apoptosis limfosit pada kelompok pasien meninggal adalah 1,5853% dengan simpang baku 0,57%. Jumlah apoptosis limfosit pada kedua kelompok berbeda bermakna dengan nilai p 0,004. Ditentukan nilai cut-off jumlah apoptosis limfosit 0,97%untuk menentukan prognosis pasien sepsis, dengan AUC 0,791 (IK 95% 0,631 ? 0,951), sensitivitas 86,7%, dan spesifisitas 60%. Kurva Kapplan Meier berdasarkan nilai cut-off 0,97% menunjukkan gambar yang memenuhi asumsi proporsional hazard dengan rasio hazard 0,182 (IK 95% 0,041 - 0,814), p = 0,026. Kami menyimpulkan jumlah apoptosis limfosit pasien sepsis berat dapat digunakan untuk memprediksi pasien yang meninggal dilihat dari mortalitas 14 hari, dengan nilai AUC sedang. Cut-off jumlah apoptosis limfosit 0,97% dapat digunakan sebagai cut-off dalam tatalaksana pasien sepsis berat

---

ABSTRACT
Sepsis is a common illness in intensive care, almost 1/3 of patients admitted to the ICU were sepsis patients. There are plenty of researches to find a reliable marker of sepsis and the number of apoptotic lymphocytes began widely studied as a marker of sepsis. Apoptosis of lymphocytes occurred from the first 24 hours after the onset sepsis. There are currently no data on whether the number of apoptotic lymphocytes can be used as a prognostic marker of sepsis. The purpose of this study was to determine whether the number of apoptotic lymphocytes can be used as a prognostic marker in patients with severe sepsis

This was a prospective prognosis study, consisting of 30 severe sepsis patients grouped based on 14-day mortality, 15 patients are survivors and 15 patients are nonsurvivors. The diagnosis of sepsis is based on a modified definition of sepsis by the International Sepsis Definitions Conference 2001. The number of apoptotic lymphocytes was calculated using flowcytometry with PerCP-labeled anti-CD45 monoclonal antibody, FITC-labeled Annexin V, and Propidium Iodide. In both groups, characteristics of subjects were recorded and the number of apoptotic lymphocytes was calculated.

The mean of apoptotic lymphocytes in the survivor group is 0.992% with a standard deviation of 0.44%, and the mean of apoptotic lymphocytes in the nonsurvivor group is 1.5853% with a standard deviation of 0.57%. The difference between the two groups is significant with p = 0.004. This study yields an apoptotic lymphocytes cut-off value of 0.97% to determine prognosis of severe sepsis patients, with AUC of 0.791 (CI 95% from 0.631 to 0.951), 86.7% sensitivity and 60% specificity. Kapplan Meier curve based on the 0.97% cut-off demonstrates that hazard proportion is fulfilled with hazard ratio of 0.182 (95% CI 0.041 to 0.814) and p= 0.026. It is concluded that the number of apoptotic lymphocytes in severe sepsis patients can be used to predict nonsurvivors based on 14-day mortality, with moderate AUC. The apoptotic lymphocytes cut-off value of 0.97% can be used as a cut-off for severe sepsis patient management

Abstrak :
Asfiksia merupakan salah satu penyebab utama morbiditas dan mortalitas neonatus. Deteksi dini asfiksia penting untuk mencegah keluaran buruk jangka pendek. Analisis gas darah tali pusat merupakan metode objektif untuk menilai hipoksia-asidosis janin yang merupakan dasar patologi asfiksia. Penelitian ini terdiri atas dua tahap. Tahap pertama merupakan penelitian comparative cross-sectional untuk menilai hubungan pO2 vena, pCO2 arteri, ΔpO2 vena-arteri, ΔpCO2 arteri-vena tali pusat, dan fractional tissue oxygen extraction dengan keluaran sekunder, yaitu dengan skor Apgar <7 pada menit ke-5. Tahap kedua menggunakan desain nested case-control untuk menilai keluaran primer, yaitu keluaran buruk jangka pendek, meliputi perdarahan intraventrikular, ensefalopati hipoksik-iskemik, perawatan neonatal intensive care unit, serta kematian neonatal dini. Total subjek adalah 47 subjek. Tahap pertama penelitian hanya mendapatkan empat kasus sehingga tidak dapat dinilai hubungan dengan skor Apgar rendah menit ke-5. Tahap kedua penelitian mendapatkan 10 kasus dan 37 kontrol. Delta pO2 vena-arteri tali pusat lebih rendah bermakna (p=0,041), sedangkan fractional tissue oxygen extraction lebih rendah namun tidak bermakna (p=0,059) pada neonatus yang mengalami keluaran buruk jangka pendek dibanding tanpa keluaran buruk. Ketiga parameter lain tidak berhubungan dengan keluaran buruk jangka pendek. Titik potong optimal untuk memprediksi keluaran buruk jangka pendek neonatus adalah ≤3,35 mmHg (Sn=83,8%; Sp=60,0%) untuk ΔpO2 vena-arteri tali pusat dan ≤16,2% (Sn=81,1%; Sp=60,0%) untuk FTOE. Delta pO2 vena-arteri tali pusat (OR=7,75 (p=0,010; IK95% 1,66 – 36,01) maupun FTOE (OR=6,43; p=0,017; IK95% 1,42 – 29,08) prediktif terhadap keluaran buruk jangka pendek neonatus. Model prediksi dibuat menggunakan parameter FTOE. ...... Asphyxia remains one of the most common cause of morbidity and mortality in neonates. Early detection is crucial to prevent asphyxia-related short-term adverse outcomes. Umbilical cord blood gas analysis provides objective measurement of fetal hypoxia and acidosis which define asphyxia. This study aimed to evaluate association of umbilical cord venous pO2, arterial pCO2, arterio-venous ΔpCO2, veno-arterial ΔpO2, and fetal fractional tissue oxygen extraction (FTOE) ratio with neonatal short-term adverse events, including intracranial hemorrhage, hypoxic-ischemic encephalopaty, admission to neonatal intensive care unit, and early neonatal death, as primary outcomes, and low 5-minute Apgar score as secondary outcomes. We used nested case-control design to evaluate primary outcomes and comparative cross-sectional design for the latter. A total of 47 subjects were recruited. Low 5-minute Apgar scores were found in four subjects, which did not fulfill the minimum sample size requirement for analysis. Short-term adverse outcomes were found in 10 cases. Delta pO2 was significantly lower (p=0,041), while FTOE was lower albeit not statistically significant (p=0,059) in case compared to control group. The other three parameters failed to show any significant associations. Optimal cutoff value for pO2 was ≤3,35 mmHg with 83,8% sensitivity dan 60,0% specificity, and ≤16,2% for FTOE (Sn=81,1%; Sp=60,0%). Either umbilical veno-arterial ΔpO2 (OR=7,75; p=0,010; 95%CI 1,66 – 36,01) or FTOE (OR=6,43; p=0,017; IK95% 1,42 – 29,08) was predictive for neonatal short-term adverse outcomes. A prediction model was developed for FTOE.

Abstrak :
Coronavirus Disease 2019 (COVID-19) disebabkan oleh infeksi virus SARS CoV-2, dan telah dilaporkan banyak menyebabkan kematian di berbagai negara. Pada pasien COVID-19, ditemukan perubahan kadar asam amino, baik asam amino esensial maupun non esensial, yang dikaitkan dengan proses inflamasi dan infeksi. Tujuan penelitian ini adalah mengetahui profil asam amino esensial dan non esensial, serta mengetahui perbedaannya pada pasien terkonfirmasi COVID-19 severe dan non severe. Penelitian dilakukan dengan metode potong lintang di RSUPN Dr. Cipto Mangunkusumo dan Laboratorium HGRC pada Januari-Desember 2021. Total subjek adalah 128 subjek, terdiri dari 70 subjek (54,7%) kelompok severe dan 58 subjek (45,3%) kelompok non severe. Profil asam amino pada pasien terkonfirmasi COVID-19 severe dan non-severe, secara klinis ditemukan sedikit perbedaan dengan rentang effect size d 0,08-0,48. Tidak terdapat perbedaan bermakna keseluruhan profil asam amino antara kelompok severe dan non severe (p>0,05). Temuan ini diharapkan dapat memberi kontribusi dalam proses penyembuhan pasien terutama pada kondisi infeksi/inflamasi akut, serta menurunkan angka morbiditas dan mortalitas melalui penambahan asupan makanan atau terapi suplementasi potensial pada penderita dengan kadar asam amino yang lebih rendah ......Coronavirus Disease 2019 (COVID-19) is an infection, caused by SARS CoV-2 virus infection, and had been reported that cause death in many countries. Patients with COVID-19 infection, could have amino acid alteration, both in essential and non essential, which are associated in inflammation and infectious processes. The main objective of this study, was to know the essential and non essential amino acid profile, and to determine the differences in severe and non severe COVID-19 patients. This cross sectional study was conducted at Dr. Cipto Mangunkusumo National Central Public Hospital and HGRC Laboratory, from January-December 2021. There were 128 subjects, consisted of 70 subjects (54.7%) in severe group and 58 subjects (45.3%) in non severe group. The amino acid profile in severe and non-severe COVID-19 patients, clinically were found slight different, with the effect size range d 0.08-0.48. There was no significant difference in all amino acid, between severe and non severe group (p>0.05). These findings were expected to contibute in recovery process especially in infection/acute inflammation state, decreased the morbidity and mortality, through additional intake and potential supplementation therapy in lower amino acid patients.

Abstrak :
RSCM merupakan rumah sakit terbesar di Indonesia yang menangani penyakit COVID-19. Ditemukan banyak faktor yang mempengaruhi morbiditas, lama perawatan dan perubahan parameter laboratorium. Prevalensi obesitas semakin meningkat di Indonesia. Saat ini belum ada penelitian mengenai pengaruh IMT terhadap derajat keparahan COVID-19 di Indonesia. Masalah penelitian ini adalah belum adanya informasi mengenai pengaruh indeks massa tubuh (IMT) terhadap derajat keparahan COVID-19 di Indonesia dan adanya perbedaan temuan hasil laboratorium (leukosit, trombosit, rasio neutrofil limfosit (RNL), PT, APTT, D-dimer dan CRP) pada pasien COVID-19 sehingga dapat memberikan informasi derajat keparahan COVID-19 pada pasien rawat inap di RSUPNCM. Penelitian ini merupakan studi potong lintang yang melibatkan 378 data subjek yang memuat data demografi, data klinis, IMT, jumlah leukosit, RNL, trombosit, PT, APTT, D-dimer, dan CRP. Analisis statistik hasil laboratorium dilakukan dengan membandingkan antar kelompok berdasarkan IMT dan derajat penyakit. Selain itu, analisis statistik juga dilakukan antara IMT dengan derajat penyakit. Penelitian ini menunjukkan adanya peningkatan bermakna nilai CRP pada kelompok overweight dan obese (53,6 mg/L dan 63,35 mg/L) dibandingkan dengan kelompok normal (22,65 mg/L). Selain itu, terdapat perbedaan bermakna jumlah leukosit, RNL, PT, APTT, D-dimer dan CRP antara kelompok severe vs non-severe pada hari pertama perawatan. Obesitas 1,88 kali lebih tinggi untuk mengalami COVID-19 derajat penyakit severe dibandingkan kelompok normal. Berdasarkan hasil penelitian ini maka obesitas merupakan faktor risiko terjadinya COVID-19 severe dimana pada kelompok obesitas didapatkan kadar CRP yang tinggi sejak hari pertama perawatan dan hanya kadar D-dimer dan CRP yang meningkat secara bermakna pada kelompok severe ......RSCM is the largest designated hospital in Indonesia for managing COVID-19 cases. Many factors have been found which affect morbidity, length of treatment and changes in laboratory parameters. The prevalence of obesity is increasing in Indonesia. Currently, there is no research on the effect of BMI on the severity of COVID-19 in Indonesia. The problem of this research is that there is no information regarding the effect of body mass index (BMI) on the severity of COVID-19 in Indonesia and the differences in laboratory findings (leukocytes count, platelets count, neutrophil to lymphocyte ratio (NLR), PT, APTT, D-dimer and CRP) in COVID-19 patients to provide information on the severity of COVID-19 in hospitalized patients at the RSUPNCM. This study is a cross-sectional study involving 378 subject data containing demographic data, clinical data, BMI, leukocyte count, NLR, platelets, PT, APTT, D-dimer, and CRP. Statistical analysis of laboratory results was performed by comparing groups based on BMI and disease severity. In addition, statistical analysis was done between BMI and disease severity. This study showed a significant increase in CRP values ​​in the overweight and obese groups (53.6 mg/L and 63.35 mg/L) compared to the normal group (22.65 mg/L). In addition, there were significant differences in the leukocytes count, NLR, PT, APTT, D-dimer and CRP between the severe vs non-severe groups on the first admission day. Obesity was 1.88 times more likely to develop severe COVID-19. Based on the results of this study, obesity is a risk factor for severe COVID-19 where in the obese group there was high CRP level since the first admission day and only D-dimer and CRP level ​increase significantly in the severe group.