Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
ABSTRAK
Dalam rangka usaha mendapatkan bibit tanaman eboni Diospyros Celebica secara besar-besaran, dilakukan percobaan tentang mikropropagasi secara teknik kultur jaringan melalui kultur meristem serta embriogenesis somatis. Meristem yang dikultur diambil dari tunas apikal dan tunas aksilar kecambah eboni umur 3 bulan. Kalus terbentuk pada medium MS dengan penambahan (2-4) ppm 2,4D + 2 ppm kinetin dan (2-4) ppm NAA + 2 ppm kinetin. Kultur suspensi embriogenesis somatik sampai usia 3 bulan masih berbentuk embryoid yang kelak akan membentuk tunas muda pada medium yang cocok. Induksi kalus untuk morfogenesis atau plantlet sampai umur 3 bulan belum berbentuk, hanya terdapat propagul-propagul pada permukaan kalus. Kultur meristem tunas apikal tumbuh baik pada medium MS + 2 ppm NAA + 8 ppm kinetin sedangkan untuk tunas aksilar pada medium MS -r- 4 ppm NAA + 8 ppm kinetin.

---

ABSTRACT
Mikropropagation Through Meristem Culture And Somatic Embryogenesis Of Ebony Diospyros Celebica BAKHIn the framework of research to get seedlings of ebony, Diospyros celebica in a large scale, a micropropagation experiment was carried on according to the tissue culture methodes through meristem culture and somatic embryogenesis. Meristem to be cut--ured were taken from the apical and axillary buds from 3 months seedlings of ebony. Calus was formed on MS medium enriched with (2-4) ppm 2,4D + 2 ppm kinetin and (2-4) ppm NAA + 2 ppm kinetin. Suspension culture of somatic embryogenesis still in embryoid from up to 3 months which later will turn to young shoots if it were grown in the appropriate medium. Callus induction for more phogenesis and plantlets gave no result up to 3 months, there were propagules only on the callus surface. Meristem culture of apical buds grew well in MS medium enriched with 2 ppm NAA + 8 ppm kinetin while meristem culture of axillary buds in MS medium enriched with 4 ppm NAA + 8 ppm kinetin.

Abstrak :
Untuk memperoleh koleksi kekayaan hayati Nostoc Indonesia dilakukan isolasi mikroflora tanah dari beberapa lahan persawahan di wilayah di Jawa, Bali, dan Sulawesi Selatan. Isolat-isolat yang tumbuh cepat diidentifikasi secara morfologi dan molekuler mengunakan sekuen parsial dari gen 16S rRNA. Walaupun hubungan kekerabatan antar isolat belum sepenuhnya dapat dijelaskan, pohon filogeni yang dihasilkan dari analisis sekuen mendukung identifikasi secara morfologi bahwa isolat-isolat yang diteliti berbeda jenis. Uji coba 6 strain Nostoc , dalam bentuk inokulum tunggal, sebagai sumber nitrogen untuk padi dilakukan. Sebanyak 2 g biomasa basah dari masing-masing strain Nostoc diinokulasi ke dalam pot-pot yang telah berisi 3 tanaman padi. Percobaan dilakukan di rumah kaca selama 115 hari. Secara statistik (ANOVA;α= 0.05) hanya strain GIA13a yang mempengaruhi panjang akar dan jumlah bulir padi bernas.

---

Abstract
In order to collect Indonesian Nostoc, isolation of soil microflora from several paddy fields in West Java, Bali, and South Celebes was carried out. Fast-growing isolates of Nostoc were selected to describe and perform molecular identification using partial sequences of 16S rRNA. The results showed that partial sequences of 16S rRNA could not resolve the phylogeny of the isolates. However, it supported the morphological studies that recognize isolates as different species of Nostoc. Potential use of Nostoc as a nitrogen source for paddy growth was carried out using six strains as single inoculums. A total biomass of 2 g (fresh weight) for each strain was inoculated, respectively, into the pot planted with three paddy plants. This experiment was conducted in the green house for 115 days. Statistical analyses (ANOVA;α= 0.05) showed that of six strains tested in this study, only strain GIA13a had influence on the augmentation of root length and the total number of filled grains.