Hasil Pencarian  ::  Simpan CSV :: Kembali

"Anti rat amniotic protein antibody was developed by immunization of rabbits with total rat amniotic protein as antigen in Freund's adjuvant. This antibody could react with both male rat serum and amniotic proteins. To obtain the pure anti AFP antibody, rabbit anti serum was loaded on an affinity chromatography column which contain agarose, activated by carbodiimide, and male rat serum protein immobilized. Eluat containing protein that was not reacting with immobilized male rat serum was collected in 2 mL fractions. Each fraction was measured. The first 8 fraction give a peak. The range of protein concentration were about 0,35 - 4,51 mg/mL. The range of anti rat .AFP antibody titre were 40 - 1280. The conclusion is that the anti rat amniotic protein antibodies contain the antibody againts rat AFP. The anti rat AFP antibody could reacted or detected the AFP in amniotin fluid proteins that was not in the adult male rat serum. The conclusion is that the anti rat amniotic protein antibodies contain the antibody againts rat AFP. The anti rat AFP antibody can react or detect the AFP in the amniotic fluid protein that is not in the adult male rat serum."

"The purpose of this study was to get optimum medium composition and agitation to trypsin-like protease production byLactobacillus plantarum FNCC 0270. The medium composition and agitation for enzyme production was optimized usingCentral Composite Design and Response Surface Method with Design Expert software version 7.1.5. Fermentation wascarried out in erlenmeyer flask at initial pH 8, 37 °C, with shaker incubator at 87.5 rpm. The results of the best of enzymeactivity 1.0 mU/mL, protein levels of 0.557 mg/mL and desirability value of 0.740. Numerical optimization was performedto approach the ideal state of the fermentation or desirability value of 1. The medium composition of fermentation usedwas: 3.64% baker's yeast, 1.21% glucose, and 0.13% skim milk. The enzyme activity reached was 1.51 mU/mL andprotein levels of 0.205 mg/mL. After numerical optimization, the fermentation process was verified using 125 mLErlenmeyer in shaking incubator at 77 rpm, initial pH 8, 37 °C, 15 h of fermentation. The verification results showed thatthe enzyme activity and protein levels was 1.273 ± 0.227 mU/mL and 0.248 ± 0.012 mg/mL, respectively.Optimization of Trypsin-like Protease Production by Lactobacillus plantarum FNCC 0270 using Response SurfaceMethodology. Tujuan penelitian ini adalah memperoleh komposisi medium dan agitasi optimum untuk produksi proteaseserupa tripsin oleh Lactobacillus plantarum FNCC 0270. Protease serupa tripsin (PST) dihasilkan oleh L. plantarumFNCC 0270 melalui proses fermentasi dengan optimasi komposisi medium dan agitasi menggunakan Central CompositeDesign dan Response Surface Methode, dengan software Design Expert versi 7.1.5. Fermentasi dilakukan dalamerlenmeyer pH awal 8 dan suhu 37 °C menggunakan shaker inkubator pada 87,5 rpm. Hasil eksperimen terbaikmenunjukkan aktivitas enzim 1 mU/mL, kadar protein 0.557 mg/mL dan diperoleh nilai desirability 0,740. Untukmendekati keadaan ideal atau nilai desirability 1 dilakukan optimasi melalui simulasi numerik, yaitu fermentasi dengankomposisi baker yeast 3,64%, kadar glukosa 1,21%, konsentrasi skim milk 0,13%, dan agitasi 77 rpm, sehingga diperolehaktivitas enzim 1,51 mU/mL dan kadar protein 0,205 mg/mL. Setelah optimasi numerik kemudian dilakukan verifikasifermentasi di laboratorium, dalam erlenmeyer menggunakan shaker inkubator, agitasi 77 rpm, pH awal 8, suhu 37 °C,selama 15 jam. Hasil verifikasi menunjukkan aktivitas enzim dan kadar protein masing-masing 1,273 ± 0,227 mU/mL dan0,248 ± 0,012 mg/mL."

"ABSTRACTBACKGROUND: Alphacalcidol, a vitamin D analog, shows immune regulatory potency as it works on the macrophage and T cell to control inflammation and T cell dysregulation in elderly. None has been known about its effect on elderly with various states of frailty syndrome, which have different level of chronic low grade inflammation. This study aimed to determine the effect of alphacalcidol on inflammatory cytokines (IL-6, IL-10, g-IFN ) and T cell subsets (CD4/CD8 ratio and CD8+ CD28-) of elderly with various stages of frailty syndrome. METHODS: from January to July 2017, a double blind randomized controlled trial (RCT) with allocation concealment, involving 110 elderly subjects from Geriatric Outpatient Clinic Cipto Mangunkusumo Hospital Jakarta, was conducted to measure the effect of 0.5 mcg alphacalcidol administration for 90 days to inflammatory cytokines (IL-6, IL-10, g-IFN) from PBMC culture supernatant, as well as CD4/CD8 and CD8+CD28- percentage using flow cytometry. Statistical analysis using SPSS version 20 was performed with t-test to measure mean difference. RESULTS: of 110 subjects involved in the RCT consisting of 27 fit, 27 pre-frail and 56 frail elderly, 25(OH)D serum level was found to be as low as 25.59 (12.2) ng/ml in alphacalcidol group and 28.27 (10.4) ng/ml in placebo group. Alphacalcidol did not decrease IL-6 (p=0.4) and g- IFN (p=0.001), but it increased IL-10 (p=0,005) and decreased IL6/IL10 ratio (p=0.008). Alphacalcidol increased CD4/CD8 ratio from 2.68 (SD 2.45) to 3.2 (SD 2.9); p=0.001 and decreased CD8+ CD28- percentage from 5.1 (SD 3.96) to 2.5 (1.5); p<0.001. Sub group analysis showed similar patterns in all frailty states. CONCLUSION: Alphacalcidol improves immune senescence by acting as anti-inflammatory agent through increased IL-10 and decreased IL6/IL-10 ratio and also improves cellular immunity through increased CD4/CD8 ratio and decreased CD8+ CD28- subset in elderly. This effect is not influenced by frailty state."

"Tujuan penelitian ini adalah untuk menilai proses penyembuhan luka dengan menggunakan ekstrak metanol daun Jatropha multifida L. berdasarkan mekanisme penurunan jumlah leukosit PMN dan peningkatan jumlah sel fibroblas.Metode: bahan yang digunakan dalam penelitian ini adalah ekstrak metanol dari daun Jatropha multifida Subyek penelitian terdiri dari 36 ekor tikus putih jantan galur Spraque Dawlay umur 2 bulan dengan berat badan sekitar 150-200 g. Hewan coba dibagi menjadi 4 kelompok. Kelompok I (negatif kontrol merupakan kelompok hewan coba yang dilukai tanpa diobati; kelompok II (kontrol positif) merupakan kelompok hewan coba yang diobati dengan Bethasone-N; Kelompok III (kontrol pelarut) merupakan kelompok yang diobati dengan alkohol 70% sedangkan kelompok IV (kelompok perlakuan) merupakan kelompok yang diobati dengan meneteskan 10 mg ekstrak metanol daun Jatropha multifida. Setiap kelompok terdiri dari 3 ekor tikus yang masing-masing dibagi lagi menjadi kelompok waktu dekapitasi pada hari ke 3, 6, dan 13. Pada jaringan luka dibuat sediaan histologi dengan pewarnaan HE dan dilanjutkan dengan menghitung jumlah leukosit PMN dan fibroblas.Pada penelitian ini memperlihatkan bahwa penurunan jumlah leukosit PMN pada kelompok perlakuan dengan ekstrak metanol daun Jatropha multifida relatif lebih baik dibandingkan dengan kontrol negatif, kontrol positif dan kontrol pelarut. Peningkatan jumlah fibroblas terjadi pada hari ke 6 dan 13 setelah perlakuan. Simpulan: ekstrak metanol daun Jatropha multifida dapat mengobati luka sayat lebih baik dibandingkan dengan kontrol negatif, kontrol positif dan kontrol pelarut.

---

Objective: The aim of this study was to evaluate the effects of methanol extract of Jatropha multifida leaves on the wound healing process and to investigate the wound healing activity based on reduced numbers of PMN (polymorpho nuclear) leukocytes and increased numbers of fibroblasts.Method: methanol extract of dried leaves of Jatropha multifida was used in the wound healing activity studies. The study subjects were 36 white male Sprague Dawlay rats aged 2 months with 150-200 gram body weight. The subjects were divided into 4 groups and experimentally injured: Group I (negative control) underwent injury without subsequent treatment; group II (positive control) received topical treatment with Bethasone-N after injury; group III (solvent control) was treated with 70% methanol; group IV (treatment group) was treated with 10 mg methanol extract of Jatropha multifida Each group consisted of 3 rats, which were decapitated on days 3, 6, and 13 after the start of treatment. Histological preparation was stained with hematoxyline-eosin (HE) and was continuously examined by counting the numbers of PMN leukocytes and fibroblasts as indicators of wound healing on days 3, 6, and 13 of treatment.The study showed lower numbers of PMN leukocytes in subjects treated with the extract of Jatropha multifidaas compared to the other groups. The numbers of fibroblasts were significantly higher on days 6 and 13 of treatment. In conclusion, the treatment of injuries with methanol extract of leaves from Jatropha multifida provided better results compared to the other groups in our study."