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"ABSTRAKPengaruh eksudat akar dan ekstrak daun Helianlhus mmuus serta Ipomoea batala' terhadap pertumbuhan rumput gajah Petmiselum po/ystacltyon telah dilakulrnn; diamati pula pertumbuhannya bila ditanam di dalam 1 pot bersama H. annuus atau l batatas.Pada penelitian ini. eksudat akar H. mvruus mampu menurunkan persentase perkecambahan biji dan panjang kecambah rumput gajah sampai 23,87 dan 47 % terhadap kontrol dalam 90 jam; sementara eksudat akar I. halalas menunjukkan penurunan 22,37% dan 23,83 %.Eksudat akar dari tanaman bunga matahari yang disiramkan selama 5 minggu pada rumput gajah umur 10 hari menekan tinggi gulma itu 13,62%; berat segar dan berat kerlng 39,56 dan 51,24 %. Eksudat akar tanarnan ubi jalar hanya berpengaruh menekan berat segardan berat kerlng gulma itu sampai 18,58 dan 18,40%.Ekstrak daun H. anmms sertal. hatatas 4 % b.k. mampu mengbambat persentase perl:ecambahan dan tinggi kecambah rumput gajah dalam 90 jam berturut-turut sebesar 38,45 dan 15,28% serta 30,79 dan 19,45%.Ekstrak daun H. annuus sertal. halalas 2% b.k.yang disiramkan sekali seminggu Rumput gajah yang ditanam dalam 1 pot bersama H. annuus atau l halatas tidak menunjukkan perbedaan nyata dalam tinggi dan berat segar rumput gajah; namun berat kering menunjukkan penurunan 33,44 dan 39,63% terhadap kontrol.

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ABSTRACT

Pennisetum polystachyon is native of Tropical Africa. It has a high reproductive capacity and rapid seed germinationit becomes a troublesome weed when it takes over waste- and cultivated lands. It is now also found along the road sides and highways in Indonesia. The seeds are wind dispersed and have a resilient ability to survive drought and certain cultural and chemical control methods.

The concept that some crop plants may be allelopathic to certain weeds is receiving increased attention in the search for alternative weed control strategies. Helianthus ammus and Ipomoea aquatica are amongst the crop plants that may have the allelopathic effect to some weeds.

This research aims to study the inhibiting potential of H. mmuus and I. batatas on the growth of P. polystachyon. This study observed the effects of root exudates and leaf extrects of H. annuus and /. batatas on the germination and growth of P. polystachyon; and also on the growth of this weed grown together with H. a1muus or I. batatas.

Root exudates taken from the sand planted with H. mmuus or I. halalaS for 4 Root exudates liquid comes out from watering H. ammus or/. batatas (01, 2, 3 and 4 plants/pot), poured over a 10-days seedling of P. polyslachyon twice a day during 5 weeks (5 replications each) reduced the height, fresh and dry weight of the weed. The height reduced up to 13.62 %; fresh and dry weight up to 39.56% and 51.24% by the root exudate of H. annuus; while those of L batatas had no effect to the height of P. polystachyon hut did reduce the fresh and dry weight up to18.58 and 18.40%.

Leaf extracts of H. anmms or I. halalas (0--4 % dry weight), with 5 replications each, reduced also the percentage of germination and length of the germination of seed ofP. polystachyon. During 90 hours, leaf extracts of H. ammus reduced the percentage of gennination of the weed species up to 38.45 % and length of the germination of seeds up to 15.28 %; while those of L halalas up to 30.79% and 19.45% respectively.

The I0 days-old seedling of P. polystachyon with SO mlleaf extracts of H. anmtus or L halalas (0; 0.5; J.O; 1.5; and 2% dry weight) once a week, showed a little difference effect on those weed growth a week after the third treatment. Leaf extract of H. anmms almost had no effect on the weed growth both in height, fresh and dry H. annuus- P. polystachyon and /. batatas- P.polystachyon grown together in a pot (0-5; 1-4; 2-3; 3-2; 4-1; and 5-0) for 5 weeks; 4 replications each, gave another result; both had no effect on height and fresh weight of P. polystachyon. H. annuus­ P. po/ystachyon and I. halalas- P. polystachyon 4-1 reduced the dry weight significantly up to 33.44 and 39.63 %."

"Telah dilakukan penelitian mengenai pola pewarisan warna bunga Zinnia elegans pada kelompok white dan red purple. Penelitian bertujuan untuk mengetahui warna bunga Z. elegans yang muncul dari parental berwarna putih (white group) dan pink keunguan (red purple group), sekaligus mengetahui pola pewarisan warna bunga Z. elegans yang tumbuh di alam. Penelitian dilakukan selama ±6 bulan dari bulan Januari sampai Juni 2020 menggunakan lima bunga, terdiri atas 2 set dari kelompok white (F0) dan 2set dari kelompok red purple (F0), dan 1 set dari kelompok red purple (F1) yang dijadikan sebagai parental. Hasil penelitian menunjukkan bahwa sifat warna bunga dari masing-masing parental diturunkan pada keturunannya. Warna baru dihasilkan dari set 1 kelompok white red purple. Warna bunga pada Zinnia elegans dapat dikelompokkan menjadi kelompok red, red purple, orange, yellow, dan white. Berdasarkan daftar warna (colour chart), masing-masing kelompok warna bunga memiliki intensitas warna yang beragam. Warna bunga yang dihasilkan dari kelompok pink keunguan lebih beragam daripada kelompok putih. Berdasarkan perhitungan chi-square, sebagian besar pola pewarisan Z. elegans yang dibiarkan tumbuh di alam mengikuti pola pewarisan dengan Z. elegans yang disilangkan oleh manusia, dan memiliki pola pewarisan yang berbeda dengan Z. elegans yang telah dimutasi.

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Research on the inheritance pattern of Zinnia elegans from white and red-purple groups has been carried out. This research studies the color of Z. elegans flowers produced from white and red-purple flowers, while knowing the inheritance pattern of Z. elegans flowers in nature. This research was conducted for about six months from January to June 2020 and used four flowers, consisting of 2 sets of white groups (F0), two sets of red-purple groups (F0), and one set of red-purple groups (F1) to be used as parental. The results show the color nature of each parent produced in their generation. New colors are produced from set 1 of the white and red-purple group. The color of flowers in Z. elegans can be grouped into red, red-purple, orange, yellow, and white. Based on the color chart, each flower color group has a variety of color intensities. The color of the flowers produced from the red-purple group is more diverse than the white group. Based on the Chi-square calculation, most of the inheritance patterns of Z. elegans that are allowed to grow in nature follow the pattern resulting from crossing by humans and have different inheritance patterns from Z. elegansobtained from mutation."

"Penelitian yang bertujuan untuk mengetahui pengaruh logam berat timbal (Pb) terhadap perkecambahan biji kangkung (Ipomoea aquatica Forsk.) telah dilakukan. Konsentrasi timbal (Pb) yang digunakan yaitu 0, 50, 100, 150, dan 200 μM. Beberapa parameter telah diamati pada uji perkecambahan. Uji perkecambahan dilakukan dengan mengecambahkan masing-masing lima belas biji kangkung dengan enam ulangan dengan media perkecambahan berupa rockwool. Data yang diperoleh kemudian dianalisis menggunakan one-way ANOVA. Hasil uji perkecambahan menunjukkan terjadinya penurunan nilai seiring bertambahnya konsentrasi timbal (Pb) terhadap parameter berat basah tajuk dan akar, panjang tajuk dan akar, indeks vigor bibit/seedling vigor index (SVI), dan indeks toleransi (TI).

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The research study aimed to determine the effect of heavy metal lead (Pb) on the germination of water spinach (Ipomoea aquatica Forsk.). Lead (Pb) concentrations of 0, 50, 100, 150, and 200 μM were used. Several parameters were observed during the germination test, which involved germinating fifteen water spinach seeds per rockwool support with six replicates. The obtained data were analyzed using one-way ANOVA. The results of the germination test showed a decrease in shoot and root fresh weight, shoot and root length, seed vigor index (SVI), and tolerance index (TI ) as the lead (Pb) concentration increased."

"ABSTRAKEfficiency of water usage is needed for sustainable agriculture. The aim of this research is to investigate the hydroponic (NFT, DFT, Aeroponic) efficiency on water use using Amaranthus viridis L. as the planted material. Efficiency on water use was measured by comparing the water use on hydroponic with the control during the plant growing period. The result shows that the three type of hydroponics are able to increase efficiency of water use. NFT is the most efficient technique with efficiency on water use 20.43%. While DFTand Aeroponic, has the efficiency on water use 12.29% and 3.57%, respectively. Enhancing efficiency of water use on hydroponic was caused by the clossing and circulating irrigation technique on hydroponic are able to minimize the evaporation . "

"ABSTRAKSweet sorghum is a kind of sorghum that contains high content of sugar in its stem. Sweet sorghum has a big potential to be developed in Indonesia owing to its wide adaptation and the fact that it can be used as raw material for liquid sugar, syrup, ethanol, and also as animal feed. Sweet sorghum has not been developed in Indonesia because of lack of a sweet sorghum variety. Improvement of available sweet sorghum genotype can be done among others through plant breeding program. First step on the plant breeding program is to increase the plant genetic variability. This might be done by introduction of varieties or by breeding to create new varieties. Induced mutation using Gamma irradiation can be used to increase the genetic variability of sweet sorghum. Mutation breeding using Gamma irradiation in sweet sorghum was aimed at improving the yield and quality of sweet sorghum. This research was conducted to study the effect of Gamma irradiation on sweet sorghum growth in the M1 generation, and to estimate the optimal dose range suitably for the breeding program. Beside, the objective of this research was to evaluate the genetic variability for the purpose of plant selection in the M2 generation. Plant materials consisted of 2 sweet sorghum lines introduced from ICRISAT namely line No. 79 and No. 83. Non-saccharin sorghum of local variety Higari was used as a control. The doses of Gamma irradiation treatment were 0, 100, 200, 300, 400, 500, 600, 700, 800, 900, and 1000 Gy. The M1 plants were sown in greenhouse at PATIR-BATAN Jakarta, and then were transplanted in the experimental field at Balitbiogen, Bogor. The M2 plants were grown in the experimental field at Lubang Buaya, Jakarta. Important agronomic traits such as plant height, spike length, stem diameter, and grain weight/spike were observed. The results indicated that sorghum lines gave different response to Gamma irradiation, and all measured variables were significantly affected. Irradiation gave morphology and physiology damages on sorghum like abnormality, sterility, and lethality in the M1 generation. The increase of irradiation doses increased physiological damage. Effective doses of Gamma irradiation for sweet sorghum was to be around 400?500 Gy, and the lethal doses 50% of sweet sorghum was around 800?1000 Gy. Putative mutation sometimes could be observed in the M2 generation. The treatment of Gamma increased genetic variability of plant height, spike length, stem diameter, and grain weight/spike. The highest genetic variability was found in the dose treatment of 200?300 Gy. Within this interval dose, there might be high probability to find desirable mutants for further breeding purpose. A number of 38 plants had been selected from the M2 population as putative mutants.

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"Penelitian dilakukan untuk mengetahui variasi bunga Hibiscus rosa-sinensis L. bentuk crested di alam, serta hubungannya dengan ekspresi gen MADS-box. Sebanyak 200 sampel bunga crested diamati secara morfologi melalui pengukuran panjang staminal column, perhitungan perhiasan bunga, dan pengamatan visual permukaan luar ovarium. Sebanyak 137 sampel bunga crested diamati secara anatomi melalui pengamatan visual struktur internal ovarium. Hasil pengamatan morfologi dan anatomi menunjukkan tingginya variasi bunga H. rosa-sinensis bentuk crested. Salah satu karakter yang menentukan tingginya variasi bunga crested di alam adalah keberadaan petal tambahan yang diduga tumbuh di lokasi keberadaan stamen dan petal akibat gejala homeosis. Homeosis pada bunga crested diduga karena tidak terekspresikannya gen C yang merupakan salah satu kelas dari gen MADS-box. Untuk membuktikan dugaan tersebut, dilakukan pengamatan molekular melalui analisis gen MADS-box yang berperan dalam proses pembungaan. Dari kelima kelas gen MADS-box yang diamati, hanya gen C yang berhasil diamplifikasi. Hasil menunjukkan bahwa gen C terekspresi di semua bagian bunga crested. Berdasarkan hasil tersebut, homeosis pada bunga crested bukan disebabkan karena tidak terekpresikannya gen C. Dibutuhkan penelitian lebih lanjut untuk mengetahui tingkat ekspresi tiap kelas gen MADS-box terhadap variasi bunga crested di alam.

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The aim of this study is to know the variation of Hibiscus rosa-sinensis L. crested flower in nature, and to know its correlation against MADS-box gene expression. The study was conducted through morphological, anatomical, and molecular observation. Morphological sections were carried out on 200 samples of flowers by measuring the length of staminal column, counting the number of perianthium, and observing the external structure of ovaries. Anatomical sections were carried out on 137 samples of flowers by observing the internal structure of ovaries. The results showed that H. rosa-sinensis crested flower has a high variety in the shape of flower. Two main parts of crested flower that effecting its variety in nature are staminodium petaloid and stamen-petal intermediate that lied in the position of stamen and petal. This phenomenon is assumed as homeotic due to the absence of C gene expression that belong to MADS-box gene family. Based on molecular observation, AGAMOUS gene (MADS-box class C) expressed in all of crested flower parts, including staminodium petaloid and stamen-petal intermediate. This results is on contradiction with the assumption that homeosis in H. rosa-sinensis caused by the absence of gene C. Further research is needed to know the expression of others MADS-box class genes expression, including their level of expression in each parts of crested flower."

"Penelitian mengenai tingkat ekspresi gen identitas bunga (SEPALLATA) dilakukan pada tiga bagian Hibiscus rosa-sinensis l., yaitu daun, epicalyx, dan kelopak bunga. Penelitian bertujuan untuk mengetahui ekpresi gen SEPALLATA pada epicalyx. Analisis tingkat ekspresi dilakukan secara kualitatif dengan metode two-steps RT-PCR dan divisualisasikan menggunakan elektroforesis agarosa. Metode modified-CTAB digunakan untuk isolasi RNA H. rosa-sinensis dan dilanjutkan dengan pemberian perlakuan DNase untuk menghilangkan gDNA yang masih tersisa. Selanjutnya, RNA diubah menjadi cDNA dengan metode Reverse Transcription dan diamplifikasi dengan metode PCR menggunakan primer spesifik. Hasil penelitian menunjukkan adanya hasil amplifikasi SEPALLATA pada epicalyx menggunakan primer GH7SEP1, namun tidak pada epicalyx menggunakan primer GH1SEP1. Konfirmasi menggunakan primer GH7SEP1 forward dan GH1SEP1 reverse tidak menunjukkan adanya hasil amplifikasi. Hasil sekuensing menunjukkan bahwa hasil amplifikasi yang didapatkan menggunakan baik primer GH1SEP1 maupun GH7SEP1 diduga kuat teramplifikasi dari gen SEPALLATA.

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Research on floral-identity gene (SEPALLATA) expression level has been done in three parts of Hibiscus rosa-sinensis; they are leaves, epicalyx and calyx. This research was conducted to observe expression of the SEPALLATA gene in epicalyx. The expression level analysis was done qualitatively by the two-steps RT-PCR and visualized using agarose electrophoresis. Hibiscus rosa-sinensis RNA was isolated using the modified-CTAB method and continued by DNase-treatment to eliminate gDNA in mixture. Furthermore, RNA was used to make cDNA using the Reverse Transcription method and amplified using the PCR method by specific primers. The result showed the presence of SEPALLATA amplification in epicalyx using GH7SEP1 primer, yet not on epicalyx using GHSEP1 primer. Confirmation using GH7SEP1 forward primer and GH1SEP1 reverse primer did not show any amplification. Sequencing and alignment results suggested that amplifications using GH1SEP1 or GH7SEP1 were allegedly, of which amplified from SEPALLATA gene."

"Today, Indonesia has the largest palm oil plantation in the world. Palm area in Indonesia, in 2008, according Ditjenbun, Department of Agriculture, estimated to reach 7 million ha (Ditjenbun Deptan 2009). Palm fruit produced by palm oil trees collected in bunches, therefore, often called as Fresh Fruit Bunches (FFB). FFBs are then transported to the factory for further process to produce palm oil. The main product of oil mill is Crude Palm Oil (CPO) and Palm Kernel Oil (PKO). In 2008, about 90,5 ton FFB yielded 18,1 ton of CPO from the fruit (mesocarp) and 2,1 ton of PKO from the nut (palm kernel) (Naibaho 1998; Ditjenbun Deptan 2009; PPKS 2010). When the nut is processed, it yields PKO, Palm Kernel Meal (PKM), and shell. The PKM is the solid part remaining from palm kernel after the extraction of PKO by a mechanical screw pressing. From 90,5 ton of processed FFB can produce about 2,5 ton of PKM. The number of this by-product always be increased due to the growth of the oil palm industry in Indonesia. Institut de Recherche pour le Développement (IRD) has done researches about the application of palm oil industry waste (PKM) as fish feed through bioconversion process using maggot. Maggot is Hermetia illucens L. larvae that has the ability to convert organic material (PKM) into it's biomass. Maggot biomass has then been used to feed fish (Hem et al 2008b). This bioconversion process of PKM using maggot produced waste, which is called Bioconversion Fertilizer Palm Kernel Meal (BFPKM). The aim of this study is to know the effect of BFPKM, as organic fertilizer, to Vigna unguiculata L. Walp (yardlong bean) var. mutiara. The study was held in Balai Riset Ikan Hias and IRD laboratorium Depok, on July until October 2008. This research is an experimental study using a randomized complete design. The parameters observed including the growth of stems, leaves, flowers, pods and roots, between the plants cultivated with BFPKM and without BFPKM. The result shows that BFPKM as organic fertilizer tend to gave a positive effect on the growth of stem, leaf, flower and pod. BFPKM also seem to stimulate the formulation of new branches and make earlier formation of flower. Inside underground, BFPKM tend to show indication to stimulate the growth of the root faster and more branching. Well root development could give more nutritions supply to whole of plant. It was showed by this study. BFPKM supplies nitrogen, phospor, and potassium for plant's growth. That nutriens are needed by plant to grow. So, this study shows that BFPKM can be applied as organic fertilizer that seem to give effect in the growth of yardlong bean."

"Telah dilakukan penelitian terhadap tiga variasi bentuk bunga Hibiscus rosa¬sinensis L. (single, crested dan double) di kampus UI depok. Hasil penelitian menunjukkan bahwa ketiganya berbeda dalam jumlah petal, stamen dan pistillum. Bunga single memiliki 5 petal, stamen 46--101 (x=78,68). Bunga crested memiliki petal tambahan berupa staminodium petaloid (7--28, x=19,01), intermediet stamen-petal (1--21, x=9,2), dan stamen (0--44, x=12). Bunga double memiliki staminodium petaloid (5--36, x=18,6), intermediet stamen-petal (0--14, x=5,32), dan stamen (3--88, x= 38). Jumlah petal tambahan berkorelasi negatif dengan jumlah stamen. Bunga single memiliki ovarium normal, bunga crested dan double dapat memiliki ovarium yang tereduksi dan bermodifikasi menjadi sepalodi. Seluruh variasi bunga H. rosa-sinensis memiliki morfologi polen yang seragam yaitu polen soliter, berbentuk globose, prolat sferoidal hingga oblat sferoidal, apertur polypantoporate, ornamentasi eksin berupa ekinet dengan ujung tumpul, membulat, bercabang dua, dan berlekuk. Ukuran polen berbanding lurus dengan ukuran bunga. Bunga single kecil memiliki polen terkecil (dv= 152,156 µm, dh= 178,312 µm), dan single besar memiliki polen terbesar (dv=174,985 µm, dh=206,023 µm). Gen AGAMOUS terekspresi pada bunga single, crested, dan double.

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The single-, crested-, double-flowers type of Hibiscus rosa sinensis L. that grown at University of Indonesia, Depok have been studied. The three varieties of flower differ in terms of additional petal, stamen number, and pistillum. Single-flowers have 5 petals, 46--101 (x = 78,68) stamens. Crested-flowers have additional petal such as staminodium petaloid 7--28 (x = 19,01), and intermediate stamen-petal 1--21 (x = 9,2), and 0--44 (x = 12) stamens. Double-flowers have 5--36 (x = 6,18) staminodium petaloid, 0--14 (x=5,32) intermediate stamen-petal, and 3--88 (x = 38) stamens. Number of additional petal negatively correlated with the number of stamenS.Si.ngle-flowers have normal ovaries. Crested-and double-flowers can have a reduced ovaries and modified into sepalodi. All of the H. rosa sinensis varieties have similarity in pollen morphology, that is solitary, globose -, spheroid prolate-, dan spheroid oblate-shaped, with polypantoporate aperture, echinate (spine) with blunt, rounded, bifurcated, and grooved apex. Pollen size has positive correlation with the size of flowers. Small single-flowers have the smallest pollen (dv = 152,156 µm, dh = 178,312 µm), and large single-flowers have the largest pollen (dv = 174,985 µm, dh = 206,023 µm). AGAMOUS gene expressed in single-, crested-, and double-flowers. "

"Telah dilakukan penelitian untuk mengetahui pengaruh campuran ekstrak tanaman binahong (Anredera cordifolia (Ten.) Steenis) dan sambiloto (Andrographis paniculata Nees) terhadap kadar glukosa darah tikus putih (Rattus norvegicus L.) jantan. Tikus dikelompokkan menjadi delapan kelompok. Kelompok kontrol normal tidak diinduksi aloksan dan diberi larutan Carboxy Methyl Cellulose (CMC). Kelompok lainnya diinduksi aloksan dosis 100 mg/kg bb secara intraperitoneal dan masing-masing diberi larutan CMC (kelompok kontrol negatif), Glibenclamide® (kelompok kontrol positif), ekstrak binahong dosis 250 mg/kg bb (kelompok perlakuan ekstrak binahong), ekstrak sambiloto dosis 500 mg/kg bb (kelompok perlakuan ekstrak sambiloto), dan campuran ekstrak dosis 750 mg/kg bb (kelompok perlakuan campuran ekstrak dosis 1); dosis 375 mg/kg bb (kelompok perlakuan campuran ekstrak dosis 2); serta dosis 187,5 mg/kg bb (kelompok campuran ekstrak dosis 3). Pemberian bahan uji dilakukan secara oral selama 21 hari berturut-turut. Hasil uji Kruskal-Wallis dan Anava 1-faktor (P < 0,05) menunjukkan bahwa ketiga dosis campuran ekstrak berpengaruh nyata terhadap penurunan rerata kadar glukosa darah. Penurunan kadar glukosa darah terbesar dicapai oleh kelompok dosis 750 mg/kg bb dengan rerata kadar glukosa darah mendekati nilai kelompok normal, yakni pada hari ke-15 sebesar 121,36 mg/dl dan pada hari ke-22 sebesar 85,37 mg/dl.

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The research was done in order to determine the effect of a mixture of extract binahong (Anredera cordifolia (Ten.) Steenis) and sambiloto (Andrographis paniculata Nees) on blood glucose levels of male white rats (Rattus norvegicus L.). The male rats were divided into eight groups. Normal control group was not induced alloxan and given Carboxy Methyl Cellulose (CMC) solution. The others were induced alloxan at dose of 100 mg/kg body weight intraperitoneally and each of them was given CMC solution (negative control group), Glibenclamide® (positive control group), binahong extract at dose of 250 mg/kg body weight (binahong group), sambiloto extract at dose of 500 mg/kg body weight (sambiloto group), and mixture extract at dose of 750 mg/kg body weight; 375 mg/kg body weight; and 187,5 mg/kg body weight. The test materials were administrated for 21 consecutive days orally. The result of this experiment showed that statistically both single and mixture extract could decrease blood glucose levels significantly (P < 0,05). The highest decrease of blood glucose levels was achieved by the mixture extract at dose of 750 mg/kg body weight with an average value of blood glucose level 121,36 mg/dl (14 days after treatment) and 85,37 mg/dl (21 days after treatment). "