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Abstrak :
ABSTRACT
The development of male hormonal contraception is based on a decrease in sperm concentration without affecting libido and sexual potency. The combination of depot medroxy progesterone acetate (DMPA) + extract of Javanese long pepper (JLP) with dosages of 0.94 mg and 1.88 mg decreas es the concentration of spermatozoa. However, it remains unknown whether the combination influences body weight, hematology, and blood biochemistry. Therefore, it is necessary to investigate the effect of DMPA + JLP extracts on the body weight, hematology, and blood biochemistry of male rats (RattusnorvegicusL.) using Sprague-Dawley strains. The research uses a completely randomized design (CRD); one group control and two treatment groups. In the first group, the castrated rats were given oral administration extracts of JLP (CJ) with doses of 0, 0.94, 1.88, 2.82, and 3.76 mg. In the second group, the rats were injected with 1.25mg DMPA and given an oral administration extract of JLP. Injection was given in week-0 and 12. Administration was conducted every day from week 7-18. Analysis of the normality and homogeneity of data is done before the ANOVA test. Data that is abnormal and not homogeneous are tested with non-parametric statistical Kruskal-Wallis. This study shows that the combination of minimal doses of DMPA and administration variousdoses of extracts of JLP does not affect body weight and hematology (erythrocyte, hemoglobin, hematocrite), and the blood biochemistry of rats, such as the values of SGPT, SGOT, HDL, and triglycerides (p< 0.05), but rather the total cholesterol and LDL (p< 0.05). Furthermore, it is concluded that the combination of the minimal dosage of DMPA and weaned various dosages of JLP extracts affect the total value and LDL cholesterol but do not influence body weight, nor hematology and blood biochemistry. Such combinations can be drawn on for asafe male contraceptive model t by taking into account the value of the total cholesterol and LDL during its use.

Abstrak :
Penelitian ini bertujuan mengetahui frekuensi mikrodelesi kromosom Y pada pria azoospermia di Indonesia. Penelitian ini menggunakan metode PCR dengan lima STS untuk melihat delesi yang timbul pada tiga subregio (AZFa, AZFb, dan AZFc) dan satu STS untuk mengamplifikasi gen SRY yang merupakan kontrol internal. Dari 35 sampel pria dengan azoospermia terdeteksi dua orang (5,7%) yang mengalami mikrodelesi pada kromosom Y (Yq). Mikrodelesi yang terdeteksi dengan enam STS adalah satu orang mengalami delesi pada sY84 (subregio AZFa) dan RBMY1 (subregio AZFb), dan satu orang mengalami delesi pada sY254 dan sY255 (subregio AZFc). Pemeriksaan delesi kromosom Y sangat dianjurkan pada pria azoospermia yang ingin mengikuti program ICSI untuk menghindarkan kelainan genetik pada keturunannya.

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Analysis of Y Chromosome Microdeletion in Indonesian Males. The aim of this study is to find out Y chromosome microdeletion in Indonesian azoospermic men. This study used the PCR method with five STS to locate deletion on three different subregions (AZFa, AZFb, and AZFc) of azoospermic men and one STS to amplify SRY gen which act as an internal control. In this study we detected two of 35 (5,7%) azoospermic men had microdeletion Yq. One had microdeletion on subregion AZFa (sY84) and AZFb (RBMY1) and the other one on subregion AZFc (sY254 and sY255). Therefore microdeletion of the Y chromosome in Indonesian azoospermic men excist. Examination of microdeletion of Y chromosomes in azoospermic men is important if they are going to participate in the Intra Cytoplasmic Infection Program to avoid genetic disorders of their descendants.