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"Pisang Cavendish (Musa acuminata Colla) merupakan salah satu kultivar pisang yang menghadapi ancaman serius berupa serangan penyakit layu Fusarium yang disebabkan oleh infeksi kapang patogen Fusarium oxysporum f. sp. cubense (Foc). Penyakit layu Fusarium menyebabkan penurunan penurunan kaualitas dan produksi tanaman pisang. Penggunaan mikroorganisme sebagai biokontrol dapat dikembangkan untuk alternatif fungisida dalam pengendalian penyakit layu Fusarium di tanaman pisang. Streptomyces spp. diketahui mampu memproduksi senyawa metabolit yang bersifat antifungi dan menekan pertumbuhan patogen. Tujuan dari penelitian ini adalah untuk memperoleh, mengetahui aktivitas biokontrol, dan mengidentifikasi isolat yang diduga sebagai Streptomyces spp. yang diisolasi dari PT Green Giant Pineapple (GGP), Lampung Timur. Metode yang dilakukan dalam penelitian ini adalah pengujian secara in vitro yang meliputi uji aktivitas enzim protease, kitinase, selulase, peroksidase, siderofor, amonia, HCN, dan katalase. Pengujian dilanjutkan dengan identifikasi secara molekuler dan uji kompatibilitas antarisolat. Isolat 1H31, 2H44, 3H32, 3H42, dan 5H5 berhasil diisolasi dari tanah Perkebunan pisang. Isolat terseleksi mampu menghambat kapang Foc dan saling kompatibel. Isolat 3H42 dan 5H5 menunjukkan aktivitas biokontrol yang paling baik yang diketahui dari hasil positif yang ditunjukkan pada semua uji aktivitas biokontrol yang dilakukan. Kelima isolat terseleksi diketahui memiliki kemiripan secara morfologis dan filogenetik dengan S. malaysiensis, S. scabei, S. cameroonensis, S. abikoensis, dan S. katrae.

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Cavendish banana (Musa acuminata Colla) is one of the banana cultivars that faces a serious threat in the form of Fusarium wilt disease caused by infection with the pathogenic fungus Fusarium oxysporum f. sp. cubense (Foc). Fusarium wilt disease causes a decrease in the quality and production of banana plants. The use of microorganisms as biocontrol can be developed as an alternative to fungicides to control Fusarium wilt disease in banana plants. Streptomyces spp. is known to produce metabolite compounds that are antifungal and suppress pathogen growth. The purpose of this study was to obtain, determine the biocontrol activity, and identify isolates suspected as Streptomyces spp. isolated from PT Green Giant Pineapple (GGP), East Lampung. The method used in this research is in vitro testing which includes enzyme activity tests of protease, chitinase, cellulase, peroxidase, siderophore, ammonia, HCN, and catalase. The test was continued with molecular identification and compatibility test between isolates. Isolates 1H31, 2H44, 3H32, 3H42, and 5H5 were successfully isolated from banana plantation soil. The selected isolates were able to inhibit Foc mold and were mutually compatible. Isolates 3H42 and 5H5 showed the best biocontrol activity known from the positive results shown in all biocontrol activity tests conducted. The five selected isolates are known to have morphological and phylogenetic similarities with S. malaysiensis, S. scabei, S. cameroonensis, S. abikoensis, and S. katrae."

"Bawang merah (Allium ascalonicum L.) adalah komoditas sayuran dengan ancaman penyakit hawar daun bakteri yang disebabkan oleh Pantoea sp. dalam produksinya. Pemanfaatan agen biokontrol dari bakteri yang berasosiasi dengan akar tanaman berpotensi tinggi menghasilkan mekanisme penghambatan patogen pada tanaman. Penelitian ini bertujuan untuk memperoleh isolat bakteri agen biokontrol yang mampu menghambat pertumbuhan patogen Pantoea sp., mempelajari karakterisasi dan mekanisme aktivitas agen biokontrol dalam penghambatan patogen Pantoea sp., serta mengidentifikasi isolat bakteri agen biokontrol. Kultur bakteri kandidat agen biokontrol diisolasi dari tanah perakaran tanaman bawang merah di lahan pertanian bawang merah di Cirebon dan Majalengka, Jawa Barat, serta Brebes, Jawa Tengah. Pengujian aktivitas meliputi uji antagonisme dan karakterisasi aktivitas biokontrol (produksi enzim protease, kitinase, selulase, siderofor, hidrogen sianida, ammonia, peroksidase dan aktivitas katalase) secara in vitro. Identifikasi isolat bakteri agen biokontrol dengan dilakukan gen 16S rRNA dan dilanjutkan dengan pembuatan rekonstruksi pohon filogenetik. Berdasarkan hasil penelitian, diperoleh 5 isolat rizobakteri (I-8, L1-9, L1-12, BM-L3(2), dan BM-L3(3)) yang memiliki kemampuan antagonis terhadap patogen Pantoea sp. penyebab penyakit hawar daun bakteri pada bawang merah. Kelima isolat tersebut menunjukkan hasil positif pada uji aktivitas enzim protease, kitinase, selulase, siderofor, hidrogen sianida, ammonia, peroksidase dan aktivitas katalase. Hanya isolat BM-L3(2) yang tidak memiliki kemampuan dalam produksi enzim kitinase. Isolat BM-L3(2) dan BM-L3(3) teridentifikasi sebagai Brevibacillus schisleri dan Bacillus sp., sedangkan isolat I-8, L1-9, dan L1-12 teridentifikasi sebagai Streptomyces rochei berdasarkan sifat morfologi, analisis sekuens 16S rRNA, dan kekerabatan pada hasil rekonstruksi pohon filogeni.

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Shallot (Allium ascalonicum L.) is vegetable commodity that has the threat of bacterial leaf blight caused by Pantoea sp. in its production. Utilization of biocontrol agents from bacteria associated with plant roots has high potential to produce pathogen inhibition mechanisms in plants. This research aims to obtain bacterial isolates of biocontrol agents that are able to inhibit the growth of Pantoea sp., study the characterization and mechanism activity of biocontrol agents, and identify bacterial of biocontrol agents. Bacterial of biocontrol agent candidates were isolated from root soil of shallot plants in Cirebon, Majalengka, and Brebes. The activity of the antagonism and biocontrol charaterization (enzyme production of protease, chitinase, cellulase, siderophore, HCN, ammonia, peroxidase and catalase) was tested by in vitro. Identification of biocontrol agent bacterial with the 16S rRNA gene was carried out and continued with phylogenetic tree reconstruction. Based on the results of the study, 5 rhizobacterial isolates (I-8, L1-9, L1-12, BM-L3(2), and BM-L3(3)) were obtained that have antagonism activity as biocontrol agent bacteria against the pathogen Pantoea sp. causing bacterial leaf blight in shallots. The five isolates showed positive results in the enzyme activity tests of protease, chitinase, cellulase, siderophore, hydrogen cyanide, ammonia, peroxidase, and catalase. Only isolate BM-L3 (2) does not have the ability to produce chitinase enzyme. Isolates BM-L3(2) and BM-L3(3) were identified as Brevibacillus schisleri and Bacillus sp., while isolates I-8, L1-9, and L1-12 were identified as Streptomyces rochei based on morphological characters, 16S rRNA sequence analysis, and kinship on phylogeny tree reconstruction results."